... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 enzyme catalysis fluorescence anisotropy decays molecular dynamics simulations mutational studies The analysis of the time-resolved anisotropy decay was done using the following model: Time...

... ( myn@iastate.edu ) 17 6 2018 6 11 2018 7 11 2018 8 11 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 acyl-ACP thioesterase enzyme catalysis enzyme mechanism fatty acid synthase site-directed mutagenesis...

... shuffling showed allosteric kinetics and enhanced detoxifying potential towards the herbicide fluorodifen. Its structure was determined. New engineered GSTs could be useful in biotechnology as efficient bioscavengers. allosteric regulation enzyme catalysis enzyme kinetics enzyme structure...

... sulfur insertions. We report the crystal structures of a lipoyl synthase complexed with S- adenosylhomocysteine or 5′-methylthioadenosine. Models based on these structures identify likely substrate-binding sites. cofactor crystal structure enzyme catalysis lipoyl synthase radical S...

... with acetylpolyamine oxidase activity. Glu 216 and Ser 218 are major determinants of spermine oxidase substrate specificity. This finding will allow for the design of specific inhibitors for these enzymes. acetylpolyamine oxidase (APAO) enzyme catalysis enzyme kinetics polyamine spermine oxidase (SMO...

... the catalytic cycle, which may exemplify a trait of E1o enzymes. bacterial metabolism decarboxylase dehydrogenase enzyme catalysis Mycobacterium protein dynamics X-ray crystallography Acyl-CoAs are the major metabolic carriers of carbon in living organisms. The most conserved mechanisms...

...Nyssa Drinkwater; Christine L. Gee; Munish Puri; Kevin R. Criscione; Michael J. McLeish; Gary L. Grunewald; Jennifer L. Martin Substrate specificity is critically important for enzyme catalysis. In the adrenaline-synthesizing enzyme PNMT (phenylethanolamine N -methyltransferase), minor changes...

... network. In the GSTD (Delta class GST) studied, adGSTD4-4, the network consisted of His 38 , Met 39 , Asn 47 , Gln 49 , His 50 and Cys 51 . In addition to contributing to glutathione binding, this region also had major effects on enzyme catalysis, as shown by changes in kinetic parameters and substrate...

... that of the selenoenzymes. 1 To whom correspondence should be addressed (email luise.krauth-siegel@bzh.uni-heidelberg.de ). 19 2 2007 13 4 2007 25 4 2007 25 4 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 enzyme catalysis glutathione peroxidase-type...

... behaviours for the two forms), the calcu- lated reaction rate can exhibit a bell-shaped dependence on surfactant concentration. The effect of the partition of enzyme and substrate is described, as is that of enzyme efficiency in the various pseudo-phases. Key words: enzyme catalysis, kinetics models...