..., resulting in enzyme inactivation. In analysis of inactivated AlkB by LC–MS/MS, several residues exhibited a mass increase of 126.1 Da, corresponding to the octanoyl moiety derived from oxidative activation of 1-octyne. Mutagenesis studies of conserved acylated residues showed that Lys 18 plays a critical...

... enzyme inactivation observed at that temperature. Hydrostatic pressure greater than 1 GPa was necessary to induce changes in the enzyme's secondary structure. No enzyme inactivation was observed at up to 700 MPa. Pressure increased PME stability towards thermal denaturation. At 200 MPa, temperatures...

... for multi-substrate enzyme reactions was developed with provision for irreversible enzyme inactivation. This model showed that two enzyme intermediates must undergo inactivation during the catalytic cycle. These forms are proposed to be PGHS compound I and a compound I–adrenaline complex. 1 To whom...

... The Biochemical Society, London ©2002 2002 α-crystallin enzyme inactivation fructation ghost cell membrane Biochem. J. (2002) 368, 865 874 (Printed in Great Britain) 865 Enzyme activity after resealing within ghost erythrocyte cells, and protection by a-crystallin against fructose-induced...