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Keywords: enzyme kinetics
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Articles
Biochem J (2020) 477 (22): 4425–4441.
Published: 27 November 2020
... properties with increased affinity for F6P [ 28 ]. Similar effects are seen in the presence of PEG [ 29 ]. enzyme kinetics human liver metabolism muscle metabolism phosphofructokinase platelet Table 2 Human PFK isoforms display distinct kinetic properties PFK-M PFK-L PFK...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (16): 2999–3018.
Published: 21 August 2020
... 7 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 buried ionizable residues cancer enzyme kinetics pH regulation post-translational modification tumor metabolism Isocitrate dehydrogenase 1 (IDH1) is a highly conserved...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (1): 99–110.
Published: 08 January 2020
... enzymes . J. Biol. Chem. 290 , 11678 – 11691 10.1074/jbc.M114.635631 35 Cornish-Bowden , A. ( 2012 ) Fundamentals of Enzyme Kinetics , 4th edn , John Wiley and Sons 36 Segel , I.H. ( 1975 ) Enzyme Kinetics: Behavior and Analysis of Rapid Equilibrium and Steady-State Enzyme...
Articles
Biochem J (2019) 476 (21): 3161–3182.
Published: 05 November 2019
... 2019 9 10 2019 10 10 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 enzyme kinetics GTPase guanylate binding protein homology modeling molecular dynamics protein purification The 65–73 kDa guanylate-binding...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (15): 2157–2172.
Published: 05 August 2019
... 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 Cel6A cellobiohydrolases cellulose enzyme kinetics GH6 hydrolysis Fungal degradation of cellulosic biomass plays a major role in the natural carbon cycle. The degradation...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (10): 1553–1570.
Published: 31 May 2019
... ], and schematic Dimplot binding interactions are displayed using LigPlot + [ 44 ]. bradykinin crystallography enzyme kinetics zinc metalloprotease Angiotensin-converting enzyme (ACE, EC 3.4.15.1) is a zinc metalloprotease with two catalytically active domains, the N- and C-domain...
Articles
Biochem J (2019) 476 (10): 1445–1463.
Published: 21 May 2019
... © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 allostery apoptosis enzyme kinetics HtrA4 serine protease XIAP High-temperature requirement protease A (HtrA) is a family of oligomeric serine proteases that are conserved from...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (4): 719–732.
Published: 28 February 2019
...@imbg.org.ua ) * These authors contributed equally to this work. 30 11 2018 28 1 2019 1 2 2019 1 2 2019 density functional theory enzyme kinetics enzyme–substrate interactions molecular dynamics thermodynamics translation factors Chiral discrimination of amino...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (23): 3779–3795.
Published: 06 December 2018
... ) 30 8 2018 26 10 2018 1 11 2018 2 11 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics methionine sulfoxide reductase molybdoenzyme oxidative stress protein oxidation proteomics Aerobic...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (20): 3221–3238.
Published: 23 October 2018
... 24 9 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 cancer metabolism enzyme inhibitor enzyme kinetics enzyme mutation IDH1 molecular dynamics Although alterations in tumor metabolism have been first described nearly...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (16): 2611–2620.
Published: 29 August 2018
... spectroscopy enzyme kinetics single-molecule intermediate trapping Objective-based total internal reflection fluorescence microscopy (TIRFM) (Olympus) equipped with 3 lasers (488 nm, 532 nm, and 640 nm diode laser system, 100 mW power), excitations and an EMCCD camera (Q-Imaging Roller Thunder) with...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2018) 475 (6): 1141–1158.
Published: 26 March 2018
...-PPase* G. sulfurreducens PCA NP954331 K + ,Na + -independent/H + No Lys460 Cl(2)-PPase C. limicola DSM245 ACD90242 Na + -regulated/H + No Lys553 * This mPPase was produced for the first time in this work. allosteric regulation cation transporter enzyme kinetics...
Articles
Biochem J (2018) 475 (4): 733–748.
Published: 16 February 2018
... against 10 mM Tris–HCl (pH 7.6) containing 10 mM NaCl and 10 µM PLP, and concentrated. crystallography enzyme kinetics enzyme structure hydrogen sulfide periodontal disease pyridoxal phosphate Periodontal diseases are associated with oral malodor [ 1 , 2 ]. Hydrogen sulfide (H 2 S) is...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (1): 137–150.
Published: 05 January 2018
... © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics lysine biosynthesis oligomerisation protein evolution Enzymes of the diaminopimelate (DAP) pathway of lysine biosynthesis are of interest as metabolic engineering targets...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (1): 225–245.
Published: 05 January 2018
.... Correspondence: A. Rod Merrill ( rmerrill@uoguelph.ca ) 23 10 2017 30 11 2017 4 12 2017 5 12 2017 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 bacterial toxins crystallography DNA modification enzyme kinetics...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (14): 2417–2433.
Published: 06 July 2017
...)] were subjected to further validation. Taken together, our data delineate some functional and biochemical properties of KLK9 for future elucidation of the role of this enzyme in health and disease. biomarkers enzyme kinetics kallikreins proteomics serine protease inhibitors substrate specificity...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (7): 1259–1272.
Published: 23 March 2017
... 2017 14 2 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme kinetics guanine nucleotide exchange factors membranes post-translational lipid modification small GTPases A vast majority of small GTPases couple their...
Articles
Biochem J (2017) 474 (2): 247–258.
Published: 06 January 2017
... 11 2016 2 11 2016 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme kinetics metal transport nitrile hydratase protein biosynthesis Nitrile hydratases (NHases, EC 4.2.1.84) are metalloenzymes that catalyze the...
Articles
Biochem J (2016) 473 (19): 3253–3267.
Published: 27 September 2016
... enzyme kinetics methylation toxicology Nicotinamide N -methyltransferase (NNMT, E.C. 2.1.1.1) N-methylates nicotinamide to 1-methylnicotinamide (MeN) using S -adenosyl methionine (SAM) as a cofactor [ 1 ]. It is widely expressed in human tissues, most strongly in the liver [ 2 ], and is up...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (16): 2471–2483.
Published: 11 August 2016
... enzyme kinetics haemolysin protein translocation RTX type I secretion system Type 1 secretion systems (T1SS) are membrane-embedded transport machineries involved in the secretion of a wide range of substrates in Gram-negative bacteria. Substrate transport occurs in one step from the cytosol...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (15): 2395–2411.
Published: 28 July 2016
.... © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 coagulation Factor IX conformational changes crystal structure enzyme kinetics substrate-binding site thermal shift assay Blood coagulation serves to react on vessel injuries and prevent...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (10): 1423–1431.
Published: 11 May 2016
...@ekmd.huji.ac.il ). 23 3 2015 17 3 2016 21 3 2016 21 3 2016 © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 ALDH1A2 enzyme kinetics retinoic acid signalling retinaldehyde dehydrogenase vitamin A metabolism Retinoic...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (9): 1267–1280.
Published: 26 April 2016
... greater than 95% purity as determined using SDS-PAGE were pooled and concentrated to ∼20 mg/ml. For MurI Msm and its mutants, all expression and purification methods were the same as MurI Mtb , except that the E. coli host was BL21(DE3). analytical ultracentrifugation enzyme kinetics...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (5): 593–603.
Published: 24 February 2016
... ceramide synthase isoforms are found in Arabidopsis thaliana each with specific substrate preferences and sensitivity to inhibitors and activators. Arabidopsis thaliana ceramide enzyme kinetics plant biochemistry sphingolipid Synthetic, codon optimized, gene constructs were custom...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (5): 559–570.
Published: 24 February 2016
... through DNA shuffling showed allosteric kinetics and enhanced detoxifying potential towards the herbicide fluorodifen. Its structure was determined. New engineered GSTs could be useful in biotechnology as efficient bioscavengers. allosteric regulation enzyme catalysis enzyme kinetics enzyme...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (3): 525–537.
Published: 22 August 2014
...–Changeux equation in which covalent modification alters the T/R (taut/relaxed) equilibrium constant, L 0 . Only L 0 depends on the phosphorylation state. enzyme kinetics glycogen synthase Monod–Wyman–Changeux rate equation parameter optimization weight estimation To date a number of...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (2): 279–289.
Published: 07 August 2014
... contributes to the ability of Legionella pneumophila to replicate within an intracellular compartment in eukaryotic cells. ATP hydrolysis bacterial infection enzyme kinetics Legionnaire’s disease nucleoside triphosphate diphosphohydrolase (NTPDase) Legionella pneumophila is the causative...
Articles
Biochem J (2014) 461 (3): 453–459.
Published: 10 July 2014
... spermine oxidase with acetylpolyamine oxidase activity. Glu 216 and Ser 218 are major determinants of spermine oxidase substrate specificity. This finding will allow for the design of specific inhibitors for these enzymes. acetylpolyamine oxidase (APAO) enzyme catalysis enzyme kinetics polyamine...
Articles
Biochem J (2014) 461 (2): 323–334.
Published: 26 June 2014
... demonstrated its chemical tractability in a compound library screen. Significantly, our data indicate that PRDM9 has broader activities than previously recognized. biochemical characterization chemical biology enzyme kinetics epigenetics high-throughput screen histone methyltransferase PRDM protein...
Includes: Supplementary data
Articles
Biochem J (2014) 459 (1): 37–45.
Published: 14 March 2014
... the interplay between the three native substrates and show how harmful oxidase activity is avoided in aerobic environments. enzyme kinetics flavin molecular evolution stopped flow thymidylate synthase time-resolved spectroscopy We exploited this spectral change to study the binding...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (2): 281–290.
Published: 28 June 2013
... addressed to either of these authors (email Giedrius.Vilkaitis@bti.vu.lt or saulius.klimasauskas@bti.vu.lt ). enzyme kinetics methyltransferase miRNA 2′- O -ribose methylation RNA modification siRNA 12 11 2012 23 4 2013 29 4 2013 29 4 2013 © The Authors Journal...
Includes: Supplementary data
Articles
Biochem J (2012) 446 (3): 489–497.
Published: 28 August 2012
... 28 6 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 bioluminescence resonance energy transfer (BRET) cytochrome P450 enzyme kinetics homomeric P450–P450 complexes protein cross-linking protein–protein interactions Cytochrome P450 plays a major role...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (3): 529–535.
Published: 29 May 2012
... mutagenesis and enzyme kinetics to analyse the binding mode of these α-isoform-selective inhibitors. The three p110α isoform-selective inhibitors have been shown to bind through three unique and different structural mechanisms, but all exhibit competitive inhibition with respect to the lipid substrate. As...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (2): 269–277.
Published: 11 May 2012
... acetylcholinesterase (AChE) Alzheimer's disease (AD) bisnorcymserine bisnoreseroline carbamate enzyme kinetics X-ray crystal structure The frequency of age-related neurodegenerative disorders and, in particular, of AD (Alzheimers's disease) is expected to greatly rise with the increasing average age of...
Includes: Supplementary data
Articles
Biochem J (2012) 443 (1): 49–56.
Published: 14 March 2012
... molecular function unlike that of its yeast and human orthologues. Arabidopsis enzyme kinetics SMAD/forkheadassociated domain tyrosyl-DNA phosphodiesterase 1 (TDP1) tyrosyl-DNA phosphodiesterase (TDP) domain vanadate analogue TDP1 (tyrosyl-DNA phosphodiesterase 1) specifically...
Includes: Supplementary data
Articles
Biochem J (2012) 442 (3): 621–629.
Published: 24 February 2012
... prenyl donors in vitro [ 2 , 4 ]. This observation is in line with the occurrence of plastoquinone species with shorter prenyl side chains in plants [ 5 , 6 ]. aromatic prenyltransferase enzyme kinetics 4-hydroxybenzoate solanesyltransferase solanesyl diphosphate (SDP) plastoquinone-9...
Includes: Supplementary data
Articles
Biochem J (2011) 440 (2): 203–215.
Published: 14 November 2011
... inhibit normal GK, whereas its TF was doubled by glucose saturation. However, the enzyme kinetics, GKRP inhibition, TF enhancement by glucose and responsiveness to GKA of the selected mutants varied greatly. Two predominant response patterns were identified accounting for nearly all mutants: (i) GK...
Includes: Supplementary data
Articles
Biochem J (2010) 430 (3): 539–549.
Published: 27 August 2010
...@imtech.res.in ). 4 1 2010 10 6 2010 24 6 2010 24 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis...
Includes: Supplementary data
Articles
Biochem J (2010) 425 (3): 585–593.
Published: 15 January 2010
... Correspondence may be addressed to either of these authors (email mmedina@unizar.es or atmartinez@cib.csic.es ) 29 9 2009 29 10 2009 5 11 2009 5 11 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 aromatic aldehyde aryl-alcohol oxidase (AAO) enzyme...
Includes: Supplementary data
Articles
Biochem J (2009) 424 (2): 169–177.
Published: 11 November 2009
...-Ara to UDP-Xyl as part of the salvage pathway for Ara. Arabidopsis bifunctionality enzyme kinetics nucleotide sugar pea ( Pisum sativum ) UDP-glucose 4-epimerase (UGE) The flux through nucleotide sugar interconversion reactions is one of the main steps controlling the amount of cell...
Includes: Supplementary data
Articles
Biochem J (2009) 422 (3): 455–462.
Published: 27 August 2009
..., NM_005420; ST2A3/hSULT2A1, NM_003167. 1 To whom correspondence should be addressed (email kyoshina@mail.pharm.tohoku.ac.jp ). 2 2 2009 4 6 2009 23 6 2009 23 6 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 cytosolic sulfotransferase enzyme...
Includes: Supplementary data
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Biochem J (2009) 421 (2): 253–261.
Published: 26 June 2009
... © The Authors Journal compilation © 2009 Biochemical Society 2009 co-activator enzyme kinetics histone H4 protein arginine N-methyltransferase 1 (PRMT1) protein arginine N-methyltransferase 2 (PRMT2) PRMT2 was discovered because of its 50% sequence similarity to the conserved...
Includes: Supplementary data
Articles
Biochem J (2007) 408 (2): 149–159.
Published: 14 November 2007
... compilation © 2007 Biochemical Society 2007 cDNA cloning enzyme kinetics protein overexpression site-directed mutagenesis structural modelling thiamine phosphate diphosphorylase In the present study, we cloned and sequenced the cDNA of a Z. mays gene designated thi3 which is...
Articles
Biochem J (2007) 402 (3): 575–580.
Published: 26 February 2007
... defects. 1 To whom correspondence should be addressed (email d.jahn@tu-bs.de ). 30 8 2006 22 11 2006 30 11 2006 30 11 2006 The Biochemical Society, London 2007 enzyme kinetics haem biosynthesis protoporphyrin protoporphyrinogen IX oxidase stopped-flow...
Articles
Biochem J (2006) 397 (1): 69–76.
Published: 14 June 2006
... as those that form a catalytic triad (Ser 10 -Asp 154 -His 157 ) and those involved in forming an oxyanion hole (Ser 10 -Gly 44 -Asn 73 ). To gain an insight into the biochemical roles of each residue, site-directed mutagenesis was employed to mutate these residues to alanine, and enzyme kinetic...
Articles
Biochem J (2006) 396 (2): 277–285.
Published: 15 May 2006
... ]). No other proteins were detected by anti-LmNMT in T. brucei lysates. Thus TbNMT is constitutively expressed and, most importantly, present in those parasites that cause disease in mammals. The Biochemical Society, London 2006 drug target enzyme kinetics Leishmania major myristoyl...
Articles
Biochem J (2006) 395 (3): 509–515.
Published: 11 April 2006
... Thr 220 . In order to remove the effect of these hydrogen bonds, four mutants (Ser 33 →Ala 33 , Asp 60 →Ala 60 , Ser 62 →Ala 62 , and Thr 220 →Ala 220 ) were constructed by site-directed mutagenesis. The results of enzyme kinetics indicated that removal of these hydrogen bonds increases the free...
Articles
Biochem J (2006) 393 (1): 389–396.
Published: 12 December 2005
... usually treated with diet alone and rarely develop diabetic-associated complications [ 12 ]. 15 7 2005 26 8 2005 21 9 2005 21 9 2005 The Biochemical Society, London 2006 diabetes mellitus enzyme kinetics GCK gene glucokinase inactivating mutation maturity-onset...
Articles
Biochem J (2005) 389 (2): 259–268.
Published: 05 July 2005
... activity. cell proliferation DNA polymerase DNA replication enzyme kinetics eukaryote resveratrol 1 To whom correspondence should be addressed (email maga@igm.cnr.it ). 13 1 2005 15 3 2005 18 3 2005 18 3 2005 The Biochemical Society, London 2005 The...
Articles
Biochem J (2004) 383 (3): 475–482.
Published: 26 October 2004
... ). 1 6 2004 7 7 2004 18 8 2004 18 8 2004 The Biochemical Society, London 2004 abasic site AIDS HIV enzyme kinetics reverse transcription Vif The vif gene of HIV-1 was initially shown to be dispensable for virus infection of transformed T-cell lines. However...