Page 1 | Search Results | Biochemical Journal

1-50 of 81

Sort by

Sort Order Select

Articles

The uncharacterized Pseudomonas aeruginosa PA4189 is a novel and efficient aminoacetaldehyde dehydrogenase

Arline Fernández-Silva, Ana L. Juárez-Vázquez, Lilian González-Segura, Javier Andrés Juárez-Díaz, Rosario A. Muñoz-Clares

Biochem J (2023) 480 (4): 259–281.

https://doi.org/10.1042/BCJ20220567

Published: 23 February 2023

... 2 2 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 bacterial metabolism enzyme kinetics molecular docking protein crystallography site-directed mutagenesis structure–function relationships In agreement with the well...

View articletitled, The uncharacterized Pseudomonas aeruginosa PA4189 is a novel and efficient aminoacetaldehyde dehydrogenase

Includes: Supplementary data

Articles

Conserved L464 in p97 D1–D2 linker is critical for p97 cofactor regulated ATPase activity

Xiaoyi Zhang, Lin Gui, Shan Li, Purbasha Nandi, Rod Carlo Columbres, Daniel E. Wong, Derek R. Moen, Henry J. Lin, Po-Lin Chiu, Tsui-Fen Chou

Biochem J (2021) 478 (17): 3185–3204.

https://doi.org/10.1042/BCJ20210288

Published: 07 September 2021

... enzyme kinetics p97/VCP p97, also known as VCP (valosin-containing protein), TER (translational endoplasmic reticulum ATPase, in mammals), VAT (valosin-containing protein-like ATPase of Thermoplasma acidophilum , in archaea) and Cdc48p (in yeast), is a ubiquitous type II ATPase protein...

View articletitled, Conserved L464 in p97 D1–D2 linker is critical for p97 cofactor regulated ATPase activity

Includes: Supplementary data

Articles

Biochemical and transcript level differences between the three human phosphofructokinases show optimisation of each isoform for specific metabolic niches

Peter M. Fernandes, James Kinkead, Iain McNae, Paul A.M. Michels, Malcolm D. Walkinshaw

Biochem J (2020) 477 (22): 4425–4441.

https://doi.org/10.1042/BCJ20200656

Published: 27 November 2020

... by the participation of University of Edinburgh in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society under a transformative agreement with JISC. enzyme kinetics human liver metabolism muscle metabolism phosphofructokinase platelet 6-Phosphofructokinase-1...

View articletitled, Biochemical and transcript level differences between the three human phosphofructokinases show optimisation of each isoform for specific metabolic niches

Includes: Supplementary data

Articles

An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity

Lucas A. Luna, Zachary Lesecq, Katharine A. White, An Hoang, David A. Scott, Olga Zagnitko, Andrey A. Bobkov, Diane L. Barber, Jamie M. Schiffer, Daniel G. Isom, Christal D. Sohl

Biochem J (2020) 477 (16): 2999–3018.

https://doi.org/10.1042/BCJ20200311

Published: 21 August 2020

... 7 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 buried ionizable residues cancer enzyme kinetics pH regulation post-translational modification tumor metabolism Isocitrate dehydrogenase 1 (IDH1) is a highly conserved...

View articletitled, An acidic residue buried in the dimer interface of isocitrate dehydrogenase 1 (IDH1) helps regulate catalysis and pH sensitivity

Includes: Supplementary data

Articles

Molecular recognition in the product site of cellobiohydrolase Cel7A regulates processive step length

Johan Pelck Olsen, Jeppe Kari, Michael Skovbo Windahl, Kim Borch, Peter Westh

Biochem J (2020) 477 (1): 99–110.

https://doi.org/10.1042/BCJ20190770

Published: 08 January 2020

.... cellobiohydrolase cellulose enzyme kinetics inhibition processivity proofreading Cellobiohydrolases (CBHs) are an industrially important group of glycoside hydrolase (GH) enzymes, which catalyze the hydrolysis of cellulose with cellobiose as the primary product. They work predominantly in a processive...

View articletitled, Molecular recognition in the product site of cellobiohydrolase Cel7A regulates processive step length

Articles

Biochemical and structural characterization of murine GBP7, a guanylate binding protein with an elongated C-terminal tail

Larissa Legewie, Jennifer Loschwitz, Nora Steffens, Martin Prescher, Xue Wang, Sander H. J. Smits, Lutz Schmitt, Birgit Strodel, Daniel Degrandi, Klaus Pfeffer

Biochem J (2019) 476 (21): 3161–3182.

https://doi.org/10.1042/BCJ20190364

Published: 05 November 2019

... 10 10 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 enzyme kinetics GTPase guanylate binding protein homology modeling molecular dynamics protein purification The 65–73 kDa guanylate-binding proteins (p65 GBPs...

View articletitled, Biochemical and structural characterization of murine GBP7, a guanylate binding protein with an elongated C-terminal tail

Includes: Supplementary data

Articles

A biochemical comparison of fungal GH6 cellobiohydrolases

Stefan Jarl Christensen, Kristian Bertel Rømer Mørkeberg Krogh, Nikolaj Spodsberg, Kim Borch, Peter Westh

Biochem J (2019) 476 (15): 2157–2172.

https://doi.org/10.1042/BCJ20190185

Published: 05 August 2019

.... Cel6A cellobiohydrolases cellulose enzyme kinetics GH6 hydrolysis Fungal degradation of cellulosic biomass plays a major role in the natural carbon cycle. The degradation process involves secretion of enzymes (cellulases) that cleave the β-1,4-glycosidic bonds in cellulose and hence...

View articletitled, A biochemical comparison of fungal GH6 cellobiohydrolases

Includes: Supplementary data

Articles

Structural basis for the C-domain-selective angiotensin-converting enzyme inhibition by bradykinin-potentiating peptide b (BPPb)

Edward D. Sturrock, Lizelle Lubbe, Gyles E. Cozier, Sylva L.U. Schwager, Afolake T. Arowolo, Lauren B. Arendse, Emma Belcher, K. Ravi Acharya

Biochem J (2019) 476 (10): 1553–1570.

https://doi.org/10.1042/BCJ20190290

Published: 31 May 2019

...) [ 32 ] (where [S] is the final substrate concentration of 0.5 mM Z-FHL). bradykinin crystallography enzyme kinetics zinc metalloprotease The mutants were expressed in CHO-K1 cells and purified to homogeneity by lisinopril affinity chromatography. Since the active site was mutated...

View articletitled, Structural basis for the C-domain-selective angiotensin-converting enzyme inhibition by bradykinin-potentiating peptide b (BPPb)

Articles

Discerning the mechanism of action of HtrA4: a serine protease implicated in the cell death pathway

Raghupathi Kummari, Shubhankar Dutta, Lalith K. Chaganti, Kakoli Bose

Biochem J (2019) 476 (10): 1445–1463.

https://doi.org/10.1042/BCJ20190224

Published: 21 May 2019

... followed by boiling for 5 min. All reaction products were analyzed on 12% Tris–tricine gel and bands were quantified using ImageJ software (1.47V, NIH, U.S.A.). For all quantitative enzyme kinetic studies, fluorescein isothiocyanate (FITC)-labelled casein (Sigma, St. Louis, MO, U.S.A.) was used...

View articletitled, Discerning the mechanism of action of HtrA4: a serine protease implicated in the cell death pathway

Includes: Supplementary data

Articles

Substrate-assisted mechanism of catalytic hydrolysis of misaminoacylated tRNA required for protein synthesis fidelity

Mykola M. Ilchenko, Mariia Yu. Rybak, Alex V. Rayevsky, Oksana P. Kovalenko, Igor Ya. Dubey, Michael A. Tukalo

Biochem J (2019) 476 (4): 719–732.

https://doi.org/10.1042/BCJ20180910

Published: 28 February 2019

... ); Michael A. Tukalo ( [email protected] ) * These authors contributed equally to this work. 30 11 2018 28 1 2019 1 2 2019 1 2 2019 © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 density functional theory enzyme...

View articletitled, Substrate-assisted mechanism of catalytic hydrolysis of misaminoacylated tRNA required for protein synthesis fidelity

Includes: Supplementary data

Articles

Rhodobacter sphaeroides methionine sulfoxide reductase P reduces R - and S -diastereomers of methionine sulfoxide from a broad-spectrum of protein substrates

Lionel Tarrago, Sandrine Grosse, Marina I. Siponen, David Lemaire, Béatrice Alonso, Guylaine Miotello, Jean Armengaud, Pascal Arnoux, David Pignol, Monique Sabaty

Biochem J (2018) 475 (23): 3779–3795.

https://doi.org/10.1042/BCJ20180706

Published: 06 December 2018

... ) or Monique Sabaty ( [email protected] ) 30 8 2018 26 10 2018 1 11 2018 2 11 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics methionine sulfoxide reductase molybdoenzyme oxidative stress protein...

View articletitled, Rhodobacter sphaeroides methionine sulfoxide reductase P reduces R - and S -diastereomers of methionine sulfoxide from a broad-spectrum of protein substrates

Includes: Supplementary data

Articles

Inhibitor potency varies widely among tumor-relevant human isocitrate dehydrogenase 1 mutants

Diego Avellaneda Matteo, Grace A. Wells, Lucas A. Luna, Adam J. Grunseth, Olga Zagnitko, David A. Scott, An Hoang, Amit Luthra, Manal A. Swairjo, Jamie M. Schiffer, Christal D. Sohl

Biochem J (2018) 475 (20): 3221–3238.

https://doi.org/10.1042/BCJ20180424

Published: 23 October 2018

... ( [email protected] ) or Christal D. Sohl ( [email protected] ) 6 6 2018 14 9 2018 18 9 2018 24 9 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 cancer metabolism enzyme inhibitor enzyme kinetics enzyme...

View articletitled, Inhibitor potency varies widely among tumor-relevant human isocitrate dehydrogenase 1 mutants

Includes: Supplementary data

Articles

Force-activated catalytic pathway accelerates bacterial adhesion against flow

Jagadish P. Hazra, Nisha Arora, Amin Sagar, Shwetha Srinivasan, Abhishek Chaudhuri, Sabyasachi Rakshit

Biochem J (2018) 475 (16): 2611–2620.

https://doi.org/10.1042/BCJ20180358

Published: 29 August 2018

... 2018 2 7 2018 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 bacterial adhesion dynamic force spectroscopy enzyme kinetics single-molecule intermediate trapping Force as a determinant of specific enzyme conformations...

View articletitled, Force-activated catalytic pathway accelerates bacterial adhesion against flow

Includes: Multimedia, Supplementary data

Articles

Role of the potassium/lysine cationic center in catalysis and functional asymmetry in membrane-bound pyrophosphatases

Erika Artukka, Heidi H. Luoto, Alexander A. Baykov, Reijo Lahti, Anssi M. Malinen

Biochem J (2018) 475 (6): 1141–1158.

https://doi.org/10.1042/BCJ20180071

Published: 26 March 2018

... Press Limited on behalf of the Biochemical Society 2018 allosteric regulation cation transporter enzyme kinetics membrane-bound pyrophosphatase protein evolution pyrophosphate Scheme 1. K + and Na + binding to the enzyme–substrate complex. Scheme 2. Substrate binding...

View articletitled, Role of the potassium/lysine cationic center in catalysis and functional asymmetry in membrane-bound pyrophosphatases

Articles

Structural insights into the catalytic mechanism of cysteine (hydroxyl) lyase from the hydrogen sulfide-producing oral pathogen, Fusobacterium nucleatum

Yuichiro Kezuka, Tetsuo Ishida, Yasuo Yoshida, Takamasa Nonaka

Biochem J (2018) 475 (4): 733–748.

https://doi.org/10.1042/BCJ20170838

Published: 16 February 2018

... of the Biochemical Society 2018 crystallography enzyme kinetics enzyme structure hydrogen sulfide periodontal disease pyridoxal phosphate Periodontal diseases are associated with oral malodor [ 1 , 2 ]. Hydrogen sulfide (H 2 S) is one of the volatile sulfur compounds that causes such malodor [ 3...

View articletitled, Structural insights into the catalytic mechanism of cysteine (hydroxyl) lyase from the hydrogen sulfide-producing oral pathogen, Fusobacterium nucleatum

Includes: Supplementary data

Articles

Characterization of the catalytic signature of Scabin toxin, a DNA-targeting ADP-ribosyltransferase

Bronwyn Lyons, Miguel R. Lugo, Stephanie Carlin, Taylor Lidster, A. Rod Merrill

Biochem J (2018) 475 (1): 225–245.

https://doi.org/10.1042/BCJ20170818

Published: 05 January 2018

... Merrill ( [email protected] ) 23 10 2017 30 11 2017 4 12 2017 5 12 2017 © 2018 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 bacterial toxins crystallography DNA modification enzyme kinetics Synthetic double...

View articletitled, Characterization of the catalytic signature of Scabin toxin, a DNA-targeting ADP-ribosyltransferase

Includes: Supplementary data

Articles

Plant DHDPR forms a dimer with unique secondary structure features that preclude higher-order assembly

Serena A.J. Watkin, Jeremy R. Keown, Eric Richards, David C. Goldstone, Sean R.A. Devenish, F. Grant Pearce

Biochem J (2018) 475 (1): 137–150.

https://doi.org/10.1042/BCJ20170709

Published: 05 January 2018

... The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2018 enzyme kinetics lysine biosynthesis oligomerisation protein evolution Enzymes of the diaminopimelate (DAP) pathway of lysine biosynthesis are of interest as metabolic engineering targets...

View articletitled, Plant DHDPR forms a dimer with unique secondary structure features that preclude higher-order assembly

Includes: Supplementary data

Articles

Biochemical and functional characterization of the human tissue kallikrein 9

Panagiota S. Filippou, Sofia Farkona, Davor Brinc, Yijing Yu, Ioannis Prassas, Eleftherios P. Diamandis

Biochem J (2017) 474 (14): 2417–2433.

https://doi.org/10.1042/BCJ20170174

Published: 06 July 2017

... biomarkers enzyme kinetics kallikreins proteomics serine protease inhibitors substrate specificity Kallikrein-related peptidases (KLKs) belong to a family of 15 closely related serine proteases with chymo(trypsin)-like specificity, encoded by the largest cluster of protease genes on chromosome...

View articletitled, Biochemical and functional characterization of the human tissue kallikrein 9

Includes: Supplementary data

Articles

Characterization of the activation of small GTPases by their GEFs on membranes using artificial membrane tethering

François Peurois, Simon Veyron, Yann Ferrandez, Ilham Ladid, Sarah Benabdi, Mahel Zeghouf, Gérald Peyroche, Jacqueline Cherfils

Biochem J (2017) 474 (7): 1259–1272.

https://doi.org/10.1042/BCJ20170015

Published: 23 March 2017

... Cherfils ( [email protected] ) 9 1 2017 3 2 2017 13 2 2017 14 2 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme kinetics guanine nucleotide exchange factors membranes post...

View articletitled, Characterization of the activation of small GTPases by their GEFs on membranes using artificial membrane tethering

Articles

The iron-type nitrile hydratase activator protein is a GTPase

Natalie Gumataotao, K.P. Wasantha Lankathilaka, Brian Bennett, Richard C. Holz

Biochem J (2017) 474 (2): 247–258.

https://doi.org/10.1042/BCJ20160884

Published: 06 January 2017

... 11 2016 2 11 2016 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 enzyme kinetics metal transport nitrile hydratase protein biosynthesis Nitrile hydratases (NHases, EC 4.2.1.84) are metalloenzymes that catalyze...

View articletitled, The iron-type nitrile hydratase activator protein is a GTPase

Articles

Nicotinamide N -methyltransferase catalyses the N-methylation of the endogenous β-carboline norharman: evidence for a novel detoxification pathway

Martin G. Thomas, Davide Sartini, Monica Emanuelli, Matthijs J. van Haren, Nathaniel I. Martin, David M. Mountford, David J. Barlow, Fábio Klamt, David B. Ramsden, Madeehah Reza, Richard B. Parsons

Biochem J (2016) 473 (19): 3253–3267.

https://doi.org/10.1042/BCJ20160219

Published: 27 September 2016

... cat / K m that was expressed as s −1 M −1 ± SEM. β-carbolines biotransformation detoxification enzyme kinetics methylation toxicology Nicotinamide N -methyltransferase (NNMT, E.C. 2.1.1.1) N-methylates nicotinamide to 1-methylnicotinamide (MeN) using S -adenosyl methionine...

View articletitled, Nicotinamide N -methyltransferase catalyses the N-methylation of the endogenous β-carboline norharman: evidence for a novel detoxification pathway

Includes: Supplementary data

Articles

Interdomain regulation of the ATPase activity of the ABC transporter haemolysin B from Escherichia coli

Sven Reimann, Gereon Poschmann, Kerstin Kanonenberg, Kai Stühler, Sander H.J. Smits, Lutz Schmitt

Biochem J (2016) 473 (16): 2471–2483.

https://doi.org/10.1042/BCJ20160154

Published: 11 August 2016

... enzyme kinetics haemolysin protein translocation RTX type I secretion system Type 1 secretion systems (T1SS) are membrane-embedded transport machineries involved in the secretion of a wide range of substrates in Gram-negative bacteria. Substrate transport occurs in one step from the cytosol...

View articletitled, Interdomain regulation of the ATPase activity of the ABC transporter haemolysin B from Escherichia coli

Includes: Supplementary data

Articles

Releasing the brakes in coagulation Factor IXa by co-operative maturation of the substrate-binding site

Line Hyltoft Kristensen, Ole H. Olsen, Grant E. Blouse, Hans Brandstetter

Biochem J (2016) 473 (15): 2395–2411.

https://doi.org/10.1042/BCJ20160336

Published: 28 July 2016

... or [email protected] ). 13 4 2016 25 4 2016 19 5 2016 19 5 2016 © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 coagulation Factor IX conformational changes crystal structure enzyme kinetics substrate-binding site...

View articletitled, Releasing the brakes in coagulation Factor IXa by co-operative maturation of the substrate-binding site

Includes: Supplementary data

Articles

Kinetic characterization and regulation of the human retinaldehyde dehydrogenase 2 enzyme during production of retinoic acid

Yehuda Shabtai, Halim Jubran, Taher Nassar, Joseph Hirschberg, Abraham Fainsod

Biochem J (2016) 473 (10): 1423–1431.

https://doi.org/10.1042/BCJ20160101

Published: 11 May 2016

[email protected] ). 23 3 2015 17 3 2016 21 3 2016 21 3 2016 © 2016 The Author(s). published by Portland Press Limited on behalf of the Biochemical Society 2016 ALDH1A2 enzyme kinetics retinoic acid signalling retinaldehyde dehydrogenase vitamin A metabolism Retinoic...

View articletitled, Kinetic characterization and regulation of the human retinaldehyde dehydrogenase 2 enzyme during production of retinoic acid

Includes: Supplementary data

Articles

Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery

Sinothai Poen, Yoshio Nakatani, Helen K. Opel-Reading, Moritz Lassé, Renwick C.J. Dobson, Kurt L. Krause

Biochem J (2016) 473 (9): 1267–1280.

https://doi.org/10.1042/BCJ20160186

Published: 26 April 2016

...). analytical ultracentrifugation enzyme kinetics glutamate racemase monomer engineering Mycobacterium tuberculosis thermofluor Mycobacterium tuberculosis is an important pathogenic bacterium that causes tuberculosis (TB). Up to one-third of the world's population is latently infected...

View articletitled, Exploring the structure of glutamate racemase from Mycobacterium tuberculosis as a template for anti-mycobacterial drug discovery

Includes: Supplementary data

Articles

Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Kyle D. Luttgeharm, Edgar B. Cahoon, Jonathan E. Markham

Biochem J (2016) 473 (5): 593–603.

https://doi.org/10.1042/BJ20150824

Published: 24 February 2016

... synthesis of ceramides acting to promote programmed cell death [ 13 – 15 ]. Arabidopsis thaliana ceramide enzyme kinetics plant biochemistry sphingolipid Ceramides are organizing components of sphingolipids in the eukaryotic cell. Three ceramide synthase isoforms are found...

View articletitled, Substrate specificity, kinetic properties and inhibition by fumonisin B 1 of ceramide synthase isoforms from Arabidopsis

Includes: Supplementary data

Articles

Directed evolution of Tau class glutathione transferases reveals a site that regulates catalytic efficiency and masks co-operativity

Irine Axarli, Abdi W. Muleta, Dimitrios Vlachakis, Sophia Kossida, Georgia Kotzia, Anastasios Maltezos, Prathusha Dhavala, Anastassios C. Papageorgiou, Nikolaos E. Labrou

Biochem J (2016) 473 (5): 559–570.

https://doi.org/10.1042/BJ20150930

Published: 24 February 2016

... shuffling showed allosteric kinetics and enhanced detoxifying potential towards the herbicide fluorodifen. Its structure was determined. New engineered GSTs could be useful in biotechnology as efficient bioscavengers. allosteric regulation enzyme catalysis enzyme kinetics enzyme structure...

View articletitled, Directed evolution of Tau class glutathione transferases reveals a site that regulates catalytic efficiency and masks co-operativity

Includes: Supplementary data

Articles

Incorporating covalent and allosteric effects into rate equations: the case of muscle glycogen synthase

Daniel C. Palm, Johann M. Rohwer, Jan-Hendrik S. Hofmeyr

Biochem J (2014) 462 (3): 525–537.

https://doi.org/10.1042/BJ20140196

Published: 22 August 2014

... for the irreversible case in the absence of modifiers is given by [ 16 ]: (2) enzyme kinetics glycogen synthase Monod–Wyman–Changeux rate equation parameter optimization weight estimation To date a number of enzymes in pathways such as glycolysis (phosphofructokinase, EC 2.7.1.11 [ 1...

View articletitled, Incorporating covalent and allosteric effects into rate equations: the case of muscle glycogen synthase

Includes: Supplementary data

Articles

Multiple ecto-nucleoside triphosphate diphosphohydrolases facilitate intracellular replication of Legionella pneumophila

Patrice Riedmaier, Fiona M. Sansom, Trifina Sofian, Travis Beddoe, Ralf Schuelein, Hayley J. Newton, Elizabeth L. Hartland

Biochem J (2014) 462 (2): 279–289.

https://doi.org/10.1042/BJ20130923

Published: 07 August 2014

... to the ability of Legionella pneumophila to replicate within an intracellular compartment in eukaryotic cells. ATP hydrolysis bacterial infection enzyme kinetics Legionnaire’s disease nucleoside triphosphate diphosphohydrolase (NTPDase) Legionella pneumophila is the causative agent...

View articletitled, Multiple ecto-nucleoside triphosphate diphosphohydrolases facilitate intracellular replication of Legionella pneumophila

Articles

The Glu 216 /Ser 218 pocket is a major determinant of spermine oxidase substrate specificity

Manuela Cervelli, Emanuela Angelucci, Pasquale Stano, Loris Leboffe, Rodolfo Federico, Giovanni Antonini, Paolo Mariottini, Fabio Polticelli

Biochem J (2014) 461 (3): 453–459.

https://doi.org/10.1042/BJ20140305

Published: 10 July 2014

... inhibitors for these enzymes. acetylpolyamine oxidase (APAO) enzyme catalysis enzyme kinetics polyamine spermine oxidase (SMO) substrate specificity The natural PAs (polyamines) Put (putrescine), Spd (spermidine) and Spm (spermine) are regulators of cell growth and proliferation...

View articletitled, The Glu 216 /Ser 218 pocket is a major determinant of spermine oxidase substrate specificity

Articles

Characterization of the histone methyltransferase PRDM9 using biochemical, biophysical and chemical biology techniques

Xiaoying Koh-Stenta, Joma Joy, Anders Poulsen, Rong Li, Yvonne Tan, Yoonjung Shim, Jung-Hyun Min, Liling Wu, Anna Ngo, Jianhe Peng, Wei Guang Seetoh, Jing Cao, John Liang Kuan Wee, Perlyn Zekui Kwek, Alvin Hung, Umayal Lakshmanan, Horst Flotow, Ernesto Guccione, Jeffrey Hill

Biochem J (2014) 461 (2): 323–334.

https://doi.org/10.1042/BJ20140374

Published: 26 June 2014

..., and demonstrated its chemical tractability in a compound library screen. Significantly, our data indicate that PRDM9 has broader activities than previously recognized. biochemical characterization chemical biology enzyme kinetics epigenetics high-throughput screen histone methyltransferase PRDM protein...

View articletitled, Characterization of the histone methyltransferase PRDM9 using biochemical, biophysical and chemical biology techniques

Includes: Supplementary data

Articles

Substrate interaction dynamics and oxygen control in the active site of thymidylate synthase ThyX

Hubert F. Becker, Kamel Djaout, Isabelle Lamarre, Jonathan E. Ulmer, Delphine Schaming, Véronique Balland, Ursula Liebl, Hannu Myllykallio, Marten H. Vos

Biochem J (2014) 459 (1): 37–45.

https://doi.org/10.1042/BJ20131567

Published: 14 March 2014

... active sites was ~50 μM. For the CH 2 H 4 folate mixing experiments, the intensity of the measuring light was lowered to avoid light-induced effects on the timescale of the measurements; therefore, the S/N (signal/noise) ratio was somewhat lower for these experiments. enzyme kinetics flavin...

View articletitled, Substrate interaction dynamics and oxygen control in the active site of thymidylate synthase ThyX

Includes: Supplementary data

Articles

Mechanistic insights into small RNA recognition and modification by the HEN1 methyltransferase

Alexandra Plotnikova, Simona Baranauskė, Aleksandr Osipenko, Saulius Klimašauskas, Giedrius Vilkaitis

Biochem J (2013) 453 (2): 281–290.

https://doi.org/10.1042/BJ20121699

Published: 28 June 2013

... recognition and modification by a representative small RNA 2′- O -methyltransferase. enzyme kinetics methyltransferase miRNA 2′- O -ribose methylation RNA modification siRNA 1 These authors made an equal contribution to this work. 2 Present address: Gregor Mendel Institute...

View articletitled, Mechanistic insights into small RNA recognition and modification by the HEN1 methyltransferase

Includes: Supplementary data

Articles

Effect of homomeric P450–P450 complexes on P450 function

James R. Reed, J. Patrick Connick, Dongmei Cheng, George F. Cawley, Wayne L. Backes

Biochem J (2012) 446 (3): 489–497.

https://doi.org/10.1042/BJ20120636

Published: 28 August 2012

... [email protected] ). 17 4 2012 26 6 2012 28 6 2012 28 6 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 bioluminescence resonance energy transfer (BRET) cytochrome P450 enzyme kinetics homomeric P450–P450 complexes protein cross-linking...

View articletitled, Effect of homomeric P450–P450 complexes on P450 function

Includes: Supplementary data

Articles

Definition of the binding mode of a new class of phosphoinositide 3-kinase α-selective inhibitors using in vitro mutagenesis of non-conserved amino acids and kinetic analysis

Zhaohua Zheng, Syazwani I. Amran, Jiuxiang Zhu, Oleg Schmidt-Kittler, Kenneth W. Kinzler, Bert Vogelstein, Peter R. Shepherd, Philip E. Thompson, Ian G. Jennings

Biochem J (2012) 444 (3): 529–535.

https://doi.org/10.1042/BJ20120499

Published: 29 May 2012

..., in the case of p110α, this has not been possible due to the fact that the only structure of a p110α–inhibitor complex determined thus far is that of the covalently bound pan-PI3K inhibitor wortmannin [ 12 ]. In the present study we have used in vitro mutagenesis and enzyme kinetics to analyse the binding...

View articletitled, Definition of the binding mode of a new class of phosphoinositide 3-kinase α-selective inhibitors using in vitro mutagenesis of non-conserved amino acids and kinetic analysis

Includes: Supplementary data

Articles

Kinetics of Torpedo californica acetylcholinesterase inhibition by bisnorcymserine and crystal structure of the complex with its leaving group

Cecilia Bartolucci, Jure Stojan, Qian-sheng Yu, Nigel H. Greig, Doriano Lamba

Biochem J (2012) 444 (2): 269–277.

https://doi.org/10.1042/BJ20111675

Published: 11 May 2012

... acetylcholinesterase (AChE) Alzheimer's disease (AD) bisnorcymserine bisnoreseroline carbamate enzyme kinetics X-ray crystal structure The frequency of age-related neurodegenerative disorders and, in particular, of AD (Alzheimers's disease) is expected to greatly rise with the increasing average age...

View articletitled, Kinetics of Torpedo californica acetylcholinesterase inhibition by bisnorcymserine and crystal structure of the complex with its leaving group

Includes: Supplementary data

Articles

Structure–function studies of a plant tyrosyl-DNA phosphodiesterase provide novel insights into DNA repair mechanisms of Arabidopsis thaliana

Hoyeun Kim, Sang Hyeon Na, So-Young Lee, Young-Min Jeong, Hyun-Ju Hwang, Jae Young Hur, Sang-Hyun Park, Je-Chang Woo, Sang-Gu Kim

Biochem J (2012) 443 (1): 49–56.

https://doi.org/10.1042/BJ20111308

Published: 14 March 2012

... glycol)] method [ 21 ]. Protoplasts were then incubated in the dark at 24°C for 24 h. Images were obtained using a confocal microscope (Radiance 2000/MP, Bio-Rad Laboratories). Arabidopsis enzyme kinetics SMAD/forkheadassociated domain tyrosyl-DNA phosphodiesterase 1 (TDP1) tyrosyl-DNA...

View articletitled, Structure–function studies of a plant tyrosyl-DNA phosphodiesterase provide novel insights into DNA repair mechanisms of Arabidopsis thaliana

Includes: Supplementary data

Articles

Plastoquinone-9 biosynthesis in cyanobacteria differs from that in plants and involves a novel 4-hydroxybenzoate solanesyltransferase

Radin Sadre, Christian Pfaff, Stephan Buchkremer

Biochem J (2012) 442 (3): 621–629.

https://doi.org/10.1042/BJ20111796

Published: 24 February 2012

... was monitored by measuring the D 600 (attenuance at 600 nm) for E. coli and the D 730 for Synechocystis sp. aromatic prenyltransferase enzyme kinetics 4-hydroxybenzoate solanesyltransferase solanesyl diphosphate (SDP) plastoquinone-9 Synechocystis sp. PCC6803 PQ-9 (plastoquinone...

View articletitled, Plastoquinone-9 biosynthesis in cyanobacteria differs from that in plants and involves a novel 4-hydroxybenzoate solanesyltransferase

Includes: Supplementary data

Articles

Mutational analysis of allosteric activation and inhibition of glucokinase

Bogumil Zelent, Stella Odili, Carol Buettger, Dorothy K. Zelent, Pan Chen, Deborah Fenner, Joseph Bass, Charles Stanley, Monique Laberge, Jane M. Vanderkooi, Ramakanth Sarabu, Joseph Grimsby, Franz M. Matschinsky

Biochem J (2011) 440 (2): 203–215.

https://doi.org/10.1042/BJ20110440

Published: 14 November 2011

... normal GK, whereas its TF was doubled by glucose saturation. However, the enzyme kinetics, GKRP inhibition, TF enhancement by glucose and responsiveness to GKA of the selected mutants varied greatly. Two predominant response patterns were identified accounting for nearly all mutants: (i) GK mutants...

View articletitled, Mutational analysis of allosteric activation and inhibition of glucokinase

Includes: Supplementary data

Articles

Intermolecular phosphotransfer is crucial for efficient catalytic activity of nucleoside diphosphate kinase

Haider Hussain Dar, Pradip K. Chakraborti

Biochem J (2010) 430 (3): 539–549.

https://doi.org/10.1042/BJ20100026

Published: 27 August 2010

... ]. © The Authors Journal compilation © 2010 Biochemical Society 2010 autophosphorylation bacterium enzyme kinetics NTP synthesis nucleoside diphosphate kinase phosphotransfer activity site-directed mutagenesis NDK is well-known as a phosphoprotein and autophosphorylation...

View articletitled, Intermolecular phosphotransfer is crucial for efficient catalytic activity of nucleoside diphosphate kinase

Includes: Supplementary data

Articles

Kinetic and chemical characterization of aldehyde oxidation by fungal aryl-alcohol oxidase

Patricia Ferreira, Aitor Hernández-Ortega, Beatriz Herguedas, Jorge Rencoret, Ana Gutiérrez, María Jesús Martínez, Jesús Jiménez-Barbero, Milagros Medina, Ángel T. Martínez

Biochem J (2010) 425 (3): 585–593.

https://doi.org/10.1042/BJ20091499

Published: 15 January 2010

... [email protected] or [email protected] ) 29 9 2009 29 10 2009 5 11 2009 5 11 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 aromatic aldehyde aryl-alcohol oxidase (AAO) enzyme kinetics gem -diol GC-MS 1 H-NMR Lignin removal...

View articletitled, Kinetic and chemical characterization of aldehyde oxidation by fungal aryl-alcohol oxidase

Includes: Supplementary data

Articles

Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants

Toshihisa Kotake, Ryohei Takata, Rajeev Verma, Masato Takaba, Daisuke Yamaguchi, Takahiro Orita, Satoshi Kaneko, Koji Matsuoka, Tetsuo Koyama, Wolf-Dieter Reiter, Yoichi Tsumuraya

Biochem J (2009) 424 (2): 169–177.

https://doi.org/10.1042/BJ20091025

Published: 11 November 2009

... Sequence Databases under accession number AB381885. 6 7 2009 3 9 2009 15 9 2009 15 9 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 Arabidopsis bifunctionality enzyme kinetics nucleotide sugar pea ( Pisum sativum ) UDP-glucose 4-epimerase...

View articletitled, Bifunctional cytosolic UDP-glucose 4-epimerases catalyse the interconversion between UDP-D-xylose and UDP-L-arabinose in plants

Includes: Supplementary data

Articles

Inhibitory effects of kynurenic acid, a tryptophan metabolite, and its derivatives on cytosolic sulfotransferases

Laddawan Senggunprai, Kouichi Yoshinari, Yasushi Yamazoe

Biochem J (2009) 422 (3): 455–462.

https://doi.org/10.1042/BJ20090168

Published: 27 August 2009

..., NM_005420; ST2A3/hSULT2A1, NM_003167. 1 To whom correspondence should be addressed (email [email protected] ). 2 2 2009 4 6 2009 23 6 2009 23 6 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 cytosolic sulfotransferase enzyme...

View articletitled, Inhibitory effects of kynurenic acid, a tryptophan metabolite, and its derivatives on cytosolic sulfotransferases

Includes: Supplementary data

Articles

Kinetic analysis of human protein arginine N-methyltransferase 2: formation of monomethyl- and asymmetric dimethyl-arginine residues on histone H4

Ted M. Lakowski, Adam Frankel

Biochem J (2009) 421 (2): 253–261.

https://doi.org/10.1042/BJ20090268

Published: 26 June 2009

... © The Authors Journal compilation © 2009 Biochemical Society 2009 co-activator enzyme kinetics histone H4 protein arginine N-methyltransferase 1 (PRMT1) protein arginine N-methyltransferase 2 (PRMT2) PRMT2 was discovered because of its 50% sequence similarity to the conserved...

View articletitled, Kinetic analysis of human protein arginine N-methyltransferase 2: formation of monomethyl- and asymmetric dimethyl-arginine residues on histone H4

Includes: Supplementary data

Articles

Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays : cDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities

Maria Rapala-Kozik, Mariusz Olczak, Katarzyna Ostrowska, Agata Starosta, Andrzej Kozik

Biochem J (2007) 408 (2): 149–159.

https://doi.org/10.1042/BJ20070677

Published: 14 November 2007

... compilation © 2007 Biochemical Society 2007 cDNA cloning enzyme kinetics protein overexpression site-directed mutagenesis structural modelling thiamine phosphate diphosphorylase The pathways of bacterial thiamine biosynthesis are well recognized (reviewed in [ 4 ]). Many of the enzymes...

View articletitled, Molecular characterization of the thi3 gene involved in thiamine biosynthesis in Zea mays : cDNA sequence and enzymatic and structural properties of the recombinant bifunctional protein with 4-amino-5-hydroxymethyl-2-methylpyrimidine (phosphate) kinase and thiamine monophosphate synthase activities

Articles

Functional definition of the tobacco protoporphyrinogen IX oxidase substrate-binding site

Ilka U. Heinemann, Nina Diekmann, Ava Masoumi, Michael Koch, Albrecht Messerschmidt, Martina Jahn, Dieter Jahn

Biochem J (2007) 402 (3): 575–580.

https://doi.org/10.1042/BJ20061321

Published: 26 February 2007

... of hydrogen peroxide. Hydrogen atoms eliminated during the reaction are written in bold letters. enzyme kinetics haem biosynthesis protoporphyrin protoporphyrinogen IX oxidase stopped-flow fluorescence variegate porphyria PPO (protoporphyrinogen IX oxidase; EC 1.3.3.4) catalyses...

View articletitled, Functional definition of the tobacco protoporphyrinogen IX oxidase substrate-binding site

Articles

Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L 1

Li-Chiun Lee, Ya-Lin Lee, Ruey-Jyh Leu, Jei-Fu Shaw

Biochem J (2006) 397 (1): 69–76.

https://doi.org/10.1042/BJ20051645

Published: 14 June 2006

... as those that form a catalytic triad (Ser 10 -Asp 154 -His 157 ) and those involved in forming an oxyanion hole (Ser 10 -Gly 44 -Asn 73 ). To gain an insight into the biochemical roles of each residue, site-directed mutagenesis was employed to mutate these residues to alanine, and enzyme kinetic studies...

View articletitled, Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L 1

Articles

Characterization and selective inhibition of myristoyl-CoA:protein N -myristoyltransferase from Trypanosoma brucei and Leishmania major

Chrysoula Panethymitaki, Paul W. Bowyer, Helen P. Price, Robin J. Leatherbarrow, Katherine A. Brown, Deborah F. Smith

Biochem J (2006) 396 (2): 277–285.

https://doi.org/10.1042/BJ20051886

Published: 15 May 2006

... extensively to 50 mM Tris/HCl (pH 7.4), prior to Resource™ Q anion-exchange chromatography (Amersham Biosciences). Following gradient elution with 0–1 M NaCl, TbNMT protein was visualized using SDS/PAGE. drug target enzyme kinetics Leishmania major myristoyl-CoA:protein N -myristoyltransferase N...

View articletitled, Characterization and selective inhibition of myristoyl-CoA:protein N -myristoyltransferase from Trypanosoma brucei and Leishmania major

Articles

Probing the importance of hydrogen bonds in the active site of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulation

Zhong-liang Zheng, Mao-qing Ye, Zhen-yu Zuo, Zhi-gang Liu, Keng-chang Tai, Guo-lin Zou

Biochem J (2006) 395 (3): 509–515.

https://doi.org/10.1042/BJ20050772

Published: 11 April 2006

... and Thr 220 . In order to remove the effect of these hydrogen bonds, four mutants (Ser 33 →Ala 33 , Asp 60 →Ala 60 , Ser 62 →Ala 62 , and Thr 220 →Ala 220 ) were constructed by site-directed mutagenesis. The results of enzyme kinetics indicated that removal of these hydrogen bonds increases the free...

View articletitled, Probing the importance of hydrogen bonds in the active site of the subtilisin nattokinase by site-directed mutagenesis and molecular dynamics simulation

Articles

Effects of novel maturity-onset diabetes of the young (MODY)-associated mutations on glucokinase activity and protein stability

María Galán, Olivier Vincent, Isabel Roncero, Sharona Azriel, Pedro Boix-Pallares, Elías Delgado-Alvarez, Francisco Díaz-Cadórniga, Enrique Blázquez, María-Angeles Navas

Biochem J (2006) 393 (1): 389–396.

https://doi.org/10.1042/BJ20051137

Published: 12 December 2005

... enzyme kinetics GCK gene glucokinase inactivating mutation maturity-onset diabetes of the young (MODY) Nearly 200 mutations spanning the complete GCK gene have been described as being involved in GK-associated pathologies (for a review, see [ 6 ]). These mutations, which are located along...

View articletitled, Effects of novel maturity-onset diabetes of the young (MODY)-associated mutations on glucokinase activity and protein stability

Update search

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only