..., we identified a restricted set of residues, N262, N332 and N448, all localized in a cluster on the outer domain of gp120, as the essential epitope for MVN binding. By generating these mutations in the corresponding HIV-1 virus, we established that the engagement of this glycan cluster with the lectin...

... from recognition by the human immune system [ 4 ]. The conserved high mannose-binding epitopes of Env, especially those in gp120, have themselves been used as target sites for infection inhibition. Glycan-binding lectin proteins, such as the cyanovirin-N (CVN) family [ 5 – 7 ] and antibodies...

... and that a significant amount of this material is PK-sensitive. 1 To whom correspondence should be addressed (email [email protected] ). 18 8 2006 19 9 2006 3 10 2006 3 10 2006 The Biochemical Society, London 2007 conformation-dependent immunoassay epitope prion protein...

... by capture–detector immunoassay. FACS showed similar levels of cell-surface PrP C on homozygous ARR (Ala 136 -Arg 154 -Arg 171 ), ARQ (Ala 136 -Arg 154 -Gln 171 ) and VRQ (Val 136 -Arg 154 -Gln 171 ) PBMCs. Cell-surface ovine PrP C showed modulation of N-terminal epitopes, which was more evident...

... of differentially sulphated saccharide epitopes. Whereas specific HS structures are commonly believed to modulate FGF-binding and activity, selective binding of defined HS epitopes to FGFs has generally not been demonstrated. In the present paper, we have identified a series of sulphated HS octasaccharide epitopes...

... cell-surface PrP C may adopt. In the present study, we have used monoclonal antibodies that recognize epitopes in either the N- or C-terminal portions of PrP to probe the conformations of PrP C on ovine PBMCs by flow cytometry. Although PBMCs from scrapie-susceptible and -resistant genotypes of sheep...

... The Biochemical Society, London ©2003 2003 conformational epitope metal ion neurodegenerative Abbreviations used: ARR, disease-resistant allelic form A 136 R 154 R 171 ; BSE, bovine spongiform encephalopathy; CJD, Creutzfeldt—Jakob disease; ECL, enhanced chemiluminescence; PBS-T20, PBS...

... was isolated and epitope mapped. Similar to its human counterpart, murine 5T4 antigen is a 72kDa glycoprotein (immunoprecipitation and Western blot analysis) and exhibits punctate cell surface expression, dependent upon the integrity of the actin cytoskeleton. Likewise, overexpression of autologous murine 5T4...