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Articles

Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome

Eleanor Barker, Amy E. Milburn, Nordine Helassa, Dean E. Hammond, Natalia Sanchez-Soriano, Alan Morgan, Jeff W. Barclay

Biochem J (2024) 481 (3): 141–160.

https://doi.org/10.1042/BCJ20230319

Published: 29 January 2024

... a transformative agreement with JISC. exocytosis neurodegeneration neuronal ceroid lipofuscinosis PC12 cell SNARE proteins STXBP1 Cysteine string protein (CSP) was initially discovered in a search for Drosophila brain-enriched proteins, and named after a cluster of cysteine residues within...

View articletitled, Proximity labelling reveals effects of disease-causing mutation on the DNAJC5/cysteine string protein α interactome

Includes: Supplementary data

Articles

Munc18a clusters SNARE-bearing liposomes prior to trans -SNARE zippering

Matthew Grant Arnold, Pratikshya Adhikari, Baobin Kang, Hao Xu (徐昊)

Biochem J (2017) 474 (19): 3339–3354.

https://doi.org/10.1042/BCJ20170494

Published: 25 September 2017

... in reconstituted exocytic reactions mediated by neuronal and non-neuronal SNAREs. We show that Munc18a plays a direct role in promoting proteoliposome clustering, underlying vesicle docking during exocytosis. In the three different fusion reactions examined, Munc18a-dependent clustering requires an intact N...

View articletitled, Munc18a clusters SNARE-bearing liposomes prior to trans -SNARE zippering

Includes: Supplementary data

Articles

α-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking

Xiaochu Lou, Jaewook Kim, Brenden J. Hawk, Yeon-Kyun Shin

Biochem J (2017) 474 (12): 2039–2049.

https://doi.org/10.1042/BCJ20170200

Published: 06 June 2017

.... Correspondence: Yeon-Kyun Shin ( [email protected] ) 12 3 2017 8 5 2017 10 5 2017 11 5 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 α-synuclein exocytosis membrane fusion single molecule SNARE proteins α...

View articletitled, α-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking

Includes: Supplementary data

Articles

Role of Munc13-4 as a Ca 2+ -dependent tether during platelet secretion

Michael C. Chicka, Qiansheng Ren, David Richards, Lance M. Hellman, Jinchao Zhang, Michael G. Fried, Sidney W. Whiteheart

Biochem J (2016) 473 (5): 627–639.

https://doi.org/10.1042/BJ20151150

Published: 24 February 2016

...Michael C. Chicka; Qiansheng Ren; David Richards; Lance M. Hellman; Jinchao Zhang; Michael G. Fried; Sidney W. Whiteheart The Munc13 family of exocytosis regulators has multiple Ca 2+ -binding, C2 domains. Here, we probed the mechanism by which Munc13-4 regulates in vitro membrane fusion...

View articletitled, Role of Munc13-4 as a Ca 2+ -dependent tether during platelet secretion

Articles

Adiponectin is released via a unique regulated exocytosis pathway from a pre-formed vesicle pool on insulin stimulation

Chun-Yan Lim, Wanjin Hong, Weiping Han

Biochem J (2015) 471 (3): 381–389.

https://doi.org/10.1042/BJ20150301

Published: 16 October 2015

... of adiponectin. Taken together, our results show that adiponectin is stored in a unique vesicular compartment, and released through a regulated exocytosis pathway that is dependent on insulin signalling. The 3T3-L1 adipocytes in 24-well plates were starved for 2 h in serum-free DMEM containing 20 mM Hepes...

View articletitled, Adiponectin is released via a unique regulated exocytosis pathway from a pre-formed vesicle pool on insulin stimulation

Includes: Multimedia, Supplementary data

Articles

Calcium modulation of exocytosis-linked plasma membrane potential oscillations in INS-1 832/13 cells

Akos A. Gerencser, Hindrik Mulder, David G. Nicholls

Biochem J (2015) 471 (1): 111–122.

https://doi.org/10.1042/BJ20150616

Published: 21 September 2015

... are not driven by upstream metabolic fluctuations, but rather by autonomous ionic mechanisms, the details of which are unclear. We have investigated the nature of the oscillator, with simultaneous fluorescence monitoring of Δψ p , [Ca 2+ ] c and exocytosis at single-cell resolution, combined with analysis...

View articletitled, Calcium modulation of exocytosis-linked plasma membrane potential oscillations in INS-1 832/13 cells

Articles

The proteins of exocytosis: lessons from the sperm model

Claudia Nora Tomes

Biochem J (2015) 465 (3): 359–370.

https://doi.org/10.1042/BJ20141169

Published: 22 January 2015

...Claudia Nora Tomes Exocytosis is a highly regulated process that consists of multiple functionally, kinetically and/or morphologically definable stages such as recruitment, targeting, tethering and docking of secretory vesicles with the plasma membrane, priming of the fusion machinery and calcium...

View articletitled, The proteins of exocytosis: lessons from the sperm model

Articles

Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Tetsuya Kitaguchi, Manami Oya, Yoshiko Wada, Takashi Tsuboi, Atsushi Miyawaki

Biochem J (2013) 450 (2): 365–373.

https://doi.org/10.1042/BJ20121022

Published: 15 February 2013

...Tetsuya Kitaguchi; Manami Oya; Yoshiko Wada; Takashi Tsuboi; Atsushi Miyawaki Intracellular cAMP and Ca 2+ are important second messengers that regulate insulin secretion in pancreatic β-cells; however, the molecular mechanism underlying their mutual interaction for exocytosis is not fully...

View articletitled, Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Includes: Supplementary data

Articles

Munc18b is an essential gene in mice whose expression is limiting for secretion by airway epithelial and mast cells

Kyubo Kim, Youlia M. Petrova, Brenton L. Scott, Rupesh Nigam, Anurag Agrawal, Christopher M. Evans, Zoulikha Azzegagh, Alejandra Gomez, Elsa M. Rodarte, Vesa M. Olkkonen, Rustam Bagirzadeh, Lucia Piccotti, Binhui Ren, Joo-Heon Yoon, James A. McNew, Roberto Adachi, Michael J. Tuvim, Burton F. Dickey

Biochem J (2012) 446 (3): 383–394.

https://doi.org/10.1042/BJ20120057

Published: 28 August 2012

..., distribution and reproduction in any medium, provided the original work is properly cited. exocytosis mast cell mucin mucus Munc18 secretion SNARE [SNAP (soluble N -ethylmaleimide-sensitive factor-attachment protein) receptor] proteins and SM (Sec1/Munc18) proteins collaborate to mediate...

View articletitled, Munc18b is an essential gene in mice whose expression is limiting for secretion by airway epithelial and mast cells

Includes: Supplementary data

Articles

Longer-acting and highly potent chimaeric inhibitors of excessive exocytosis created with domains from botulinum neurotoxin A and B

Jiafu Wang, Tomas H. Zurawski, MacDara O. Bodeker, Jianghui Meng, Sanjay Boddul, K. Roger Aoki, J. Oliver Dolly

Biochem J (2012) 444 (1): 59–67.

https://doi.org/10.1042/BJ20120100

Published: 26 April 2012

... ability to reduce exocytosis from non-neuronal cells expressing the BoNT/A-acceptor and utilising VAMP, but not SNAP-25, in exocytosis. Hyper-excitability disorders of cholinergically innervated skeletal and smooth muscles are treatable with BoNTs (botulinum neurotoxins) [ 1 ]. There are seven serotypes...

View articletitled, Longer-acting and highly potent chimaeric inhibitors of excessive exocytosis created with domains from botulinum neurotoxin A and B

Articles

Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells

Kyota Aoyagi, Mica Ohara-Imaizumi, Chiyono Nishiwaki, Yoko Nakamichi, Shinya Nagamatsu

Biochem J (2010) 432 (2): 375–386.

https://doi.org/10.1042/BJ20100864

Published: 12 November 2010

... remains unclear. In the present study, we investigated the physiological role of insulin and PI3K in glucose-induced biphasic insulin exocytosis in primary cultured β-cells and insulinoma Min6 cells using total internal reflection fluorescent microscopy. The pretreatment of β-cells with insulin induced...

View articletitled, Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells

Articles

Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis

Ryo Aoki, Tetsuya Kitaguchi, Manami Oya, Yu Yanagihara, Mai Sato, Atsushi Miyawaki, Takashi Tsuboi

Biochem J (2010) 429 (3): 497–504.

https://doi.org/10.1042/BJ20091839

Published: 14 July 2010

...Ryo Aoki; Tetsuya Kitaguchi; Manami Oya; Yu Yanagihara; Mai Sato; Atsushi Miyawaki; Takashi Tsuboi Since the fusion pore of the secretory vesicle is resealed before complete dilation during ‘kiss-and-run’ exocytosis, their cargoes are not completely released. Although the transient fusion pore...

View articletitled, Duration of fusion pore opening and the amount of hormone released are regulated by myosin II during kiss-and-run exocytosis

Includes: Supplementary data

Articles

The highly conserved synapsin domain E mediates synapsin dimerization and phospholipid vesicle clustering

Ilaria Monaldi, Massimo Vassalli, Angela Bachi, Silvia Giovedì, Enrico Millo, Flavia Valtorta, Roberto Raiteri, Fabio Benfenati, Anna Fassio

Biochem J (2010) 426 (1): 55–64.

https://doi.org/10.1042/BJ20090762

Published: 27 January 2010

... was shown to contribute to several synapsin functions, ranging from formation of the SV reserve pool to regulation of the kinetics of exocytosis and SV cycling, although the molecular mechanisms underlying these effects are unknown. In the present study, we used a synthetic 25-mer peptide encompassing...

View articletitled, The highly conserved synapsin domain E mediates synapsin dimerization and phospholipid vesicle clustering

Articles

Cholesterol, regulated exocytosis and the physiological fusion machine

Matthew A. Churchward, Jens R. Coorssen

Biochem J (2009) 423 (1): 1–14.

https://doi.org/10.1042/BJ20090969

Published: 14 September 2009

...Matthew A. Churchward; Jens R. Coorssen Exocytosis is a highly conserved and essential process. Although numerous proteins are involved throughout the exocytotic process, the defining membrane fusion step appears to occur through a lipid-dominated mechanism. Here we review and integrate the current...

View articletitled, Cholesterol, regulated exocytosis and the physiological fusion machine

Articles

Specific effects of KChIP3/calsenilin/DREAM, but not KChIPs 1, 2 and 4, on calcium signalling and regulated secretion in PC12 cells

Neil Venn, Lee P. Haynes, Robert D. Burgoyne

Biochem J (2008) 413 (1): 71–80.

https://doi.org/10.1042/BJ20080441

Published: 12 June 2008

... of Kv4.2 channels to the plasma membrane. KChIP3 expression in PC12 cells resulted in an increase in exocytosis evoked by activation of purinergic receptors. In contrast, KChIPs 1, 2 and 4, although expressed to the same extent, had no effect on secretion. In addition, KChIP3 but not KChIPs 1, 2 and 4...

View articletitled, Specific effects of KChIP3/calsenilin/DREAM, but not KChIPs 1, 2 and 4, on calcium signalling and regulated secretion in PC12 cells

Articles

Insulin exocytosis in Goto-Kakizaki rat β-cells subjected to long-term glinide or sulfonylurea treatment

Junko Kawai, Mica Ohara-Imaizumi, Yoko Nakamichi, Tadashi Okamura, Yoshihiro Akimoto, Satsuki Matsushima, Kyota Aoyagi, Hayato Kawakami, Takashi Watanabe, Hirotaka Watada, Ryuzo Kawamori, Shinya Nagamatsu

Biochem J (2008) 412 (1): 93–101.

https://doi.org/10.1042/BJ20071282

Published: 25 April 2008

... release occurred with glibenclamide treatment. In accord with the perifusion results, TIRF (total internal reflection fluorescence) imaging of insulin exocytosis showed restoration of the decreased number of docked insulin granules and the fusion events from them during first-phase release for nateglinide...

View articletitled, Insulin exocytosis in Goto-Kakizaki rat β-cells subjected to long-term glinide or sulfonylurea treatment

Includes: Multimedia, Supplementary data

Articles

A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3

Margaret E. Graham, Mark T. W. Handley, Jeff W. Barclay, Leo F. Ciufo, Stephanie L. Barrow, Alan Morgan, Robert D. Burgoyne

Biochem J (2008) 409 (2): 407–416.

https://doi.org/10.1042/BJ20071094

Published: 21 December 2007

...Margaret E. Graham; Mark T. W. Handley; Jeff W. Barclay; Leo F. Ciufo; Stephanie L. Barrow; Alan Morgan; Robert D. Burgoyne Munc18-1 plays a crucial role in regulated exocytosis in neurons and neuroendocrine cells through modulation of vesicle docking and membrane fusion. The molecular basis...

View articletitled, A gain-of-function mutant of Munc18-1 stimulates secretory granule recruitment and exocytosis and reveals a direct interaction of Munc18-1 with Rab3

Articles

Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2

Dario Bonanomi, Laura Rusconi, Chiara Agnese Colombo, Fabio Benfenati, Flavia Valtorta

Biochem J (2007) 404 (3): 525–534.

https://doi.org/10.1042/BJ20061907

Published: 29 May 2007

...°C in the presence of FITC-conjugated transferrin (30 μg/ml). After incubation, cells were subjected to acid-stripping at 4°C as described above to remove surface-associated transferrin before fixation. endocytosis exocytosis synaptobrevin 2 synaptophysin I trafficking vesicle-associated...

View articletitled, Synaptophysin I selectively specifies the exocytic pathway of synaptobrevin 2/VAMP2

Includes: Supplementary data

Articles

Distinct roles of the C 2 A and the C 2 B domain of the vesicular Ca 2+ sensor synaptotagmin 9 in endocrine β-cells

Florence Grise, Nada Taib, Carole Monterrat, Valérie Lagrée, Jochen Lang

Biochem J (2007) 403 (3): 483–492.

https://doi.org/10.1042/BJ20061182

Published: 12 April 2007

...Florence Grise; Nada Taib; Carole Monterrat; Valérie Lagrée; Jochen Lang Synaptotagmins form a family of calcium-sensor proteins implicated in exocytosis, and these vesicular transmembrane proteins are endowed with two cytosolic calcium-binding C 2 domains, C 2 A and C 2 B. Whereas the isoforms...

View articletitled, Distinct roles of the C 2 A and the C 2 B domain of the vesicular Ca 2+ sensor synaptotagmin 9 in endocrine β-cells

Articles

Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes

Daniela B. Munafó, Jennifer L. Johnson, Beverly A. Ellis, Sophie Rutschmann, Bruce Beutler, Sergio D. Catz

Biochem J (2007) 402 (2): 229–239.

https://doi.org/10.1042/BJ20060950

Published: 12 February 2007

... a set of membrane proteins that are translocated to the plasma membrane during activation thus replenishing the plasma membrane with diverse receptor molecules that are essential for neutrophil function [ 7 ]. It is generally accepted that granule exocytosis during neutrophil activation is hierarchical...

View articletitled, Rab27a is a key component of the secretory machinery of azurophilic granules in granulocytes

Articles

Physiological stimulation regulates the exocytic mode through calcium activation of protein kinase C in mouse chromaffin cells

Tiberiu Fulop, Corey Smith

Biochem J (2006) 399 (1): 111–119.

https://doi.org/10.1042/BJ20060654

Published: 13 September 2006

... kinase C, which then promotes fusion pore dilation until full granule collapse occurs. Our results demonstrate that the transition between ‘kiss and run’ and ‘full collapse’ exocytosis serves a vital physiological regulation in neuroendocrine chromaffin cells and help effect a proper acute stress...

View articletitled, Physiological stimulation regulates the exocytic mode through calcium activation of protein kinase C in mouse chromaffin cells

Articles

The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase

Jennifer L. Johnson, Beverly A. Ellis, Deborah Noack, Miguel C. Seabra, Sergio D. Catz

Biochem J (2005) 391 (3): 699–710.

https://doi.org/10.1042/BJ20050380

Published: 25 October 2005

... (phosphoinositide 3-kinase) inhibitor. This supports the view that PI3K plays a differential role in the secretion of prostate secretory markers. In conclusion, we present evidence that JFC1 differentially regulates the secretion of PSAP and PSA, and that Rab27a and PI3K play a central role in the exocytosis...

View articletitled, The Rab27a-binding protein, JFC1, regulates androgen-dependent secretion of prostate-specific antigen and prostatic-specific acid phosphatase

Articles

Open form of syntaxin-1A is a more potent inhibitor than wild-type syntaxin-1A of Kv2.1 channels

Yuk M. LEUNG, Youhou KANG, Fuzhen XIA, Laura SHEU, Xiaodong GAO, Huanli XIE, Robert G. TSUSHIMA, Herbert Y. GAISANO

Biochem J (2005) 387 (1): 195–202.

https://doi.org/10.1042/BJ20041625

Published: 22 March 2005

...Yuk M. LEUNG; Youhou KANG; Fuzhen XIA; Laura SHEU; Xiaodong GAO; Huanli XIE; Robert G. TSUSHIMA; Herbert Y. GAISANO We have shown that SNARE (soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor) proteins not only participate directly in exocytosis, but also regulate...

View articletitled, Open form of syntaxin-1A is a more potent inhibitor than wild-type syntaxin-1A of Kv2.1 channels

Articles

Syncollin is required for efficient zymogen granule exocytosis

Barbara WÄSLE, Matthew TURVEY, Olga LARINA, Peter THORN, Jeremy SKEPPER, A. Jennifer MORTON, J. Michael EDWARDSON

Biochem J (2005) 385 (3): 721–727.

https://doi.org/10.1042/BJ20041064

Published: 24 January 2005

... syncollin KO mice was reduced by 50%. This reduction was accounted for predominantly by a loss of later, ‘secondary’ fusion events between zymogen granules and other granules that had already fused with the plasma membrane. We conclude that syncollin is required for efficient exocytosis in the pancreatic...

View articletitled, Syncollin is required for efficient zymogen granule exocytosis

Includes: Multimedia, Supplementary data

Articles

TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Mica OHARA-IMAIZUMI, Chiyono NISHIWAKI, Toshiteru KIKUTA, Shintaro NAGAI, Yoko NAKAMICHI, Shinya NAGAMATSU

Biochem J (2004) 381 (1): 13–18.

https://doi.org/10.1042/BJ20040434

Published: 22 June 2004

... exocytosis insulin release membrane fusion pancreatic β-cell total internal reflection fluorescence microscopy (TIRFM) Imaging techniques are powerful tools for detecting vesicle trafficking in live cells and they have provided significant advances in understanding the mechanism of exocytosis [ 1...

View articletitled, TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Includes: Multimedia, Supplementary data

Articles

Syncollin inhibits regulated corticotropin secretion from AtT-20 cells through a reduction in the secretory vesicle population

Barbara WÄSLE, Lori B. HAYS, Christopher J. RHODES, J. Michael EDWARDSON

Biochem J (2004) 380 (3): 897–905.

https://doi.org/10.1042/bj20031726

Published: 15 June 2004

... and appeared to exist as homooligomers. Hence, the lack of effect of syncollin–GFP on regulated exocytosis suggests that the GFP tag interferes in a subtler manner with the properties of the assembled protein. 1 To whom correspondence should be addressed (e-mail [email protected] ). 13 11 2003...

View articletitled, Syncollin inhibits regulated corticotropin secretion from AtT-20 cells through a reduction in the secretory vesicle population

Articles

RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells

Mitsunori FUKUDA

Biochem J (2004) 380 (3): 875–879.

https://doi.org/10.1042/bj20040096

Published: 15 June 2004

...Mitsunori FUKUDA Although PC12 cells express three synaptotagmin isoforms (Syts I, IV and IX), all of which have been proposed to regulate dense-core vesicle exocytosis, it remains unknown which of the Sytisoforms acts as the major Ca 2+ sensor for dense-core vesicle exocytosis. In the present...

View articletitled, RNA interference-mediated silencing of synaptotagmin IX, but not synaptotagmin I, inhibits dense-core vesicle exocytosis in PC12 cells

Articles

Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells

Penelope BUXTON, Xiang-Ming ZHANG, Bong WALSH, Absorn SRIRATANA, Irina SCHENBERG, Elizabeth MANICKAM, Tony ROWE

Biochem J (2003) 375 (2): 433–440.

https://doi.org/10.1042/bj20030427

Published: 15 October 2003

... vesicle exocytosis. Several accessory factors have been described that associate with the synaptic SNAREs and modulate core complex assembly or mediate Ca 2+ regulation. One such factor, Snapin, has been reported to be a brain-specific protein that interacts with SNAP25, and regulates association...

View articletitled, Identification and characterization of Snapin as a ubiquitously expressed SNARE-binding protein that interacts with SNAP23 in non-neuronal cells

Articles

The role of serine/threonine protein phosphatases in exocytosis

Alistair T. R. SIM, Monique L. BALDWIN, John A. P. ROSTAS, Jeff HOLST, Russell I. LUDOWYKE

Biochem J (2003) 373 (3): 641–659.

https://doi.org/10.1042/bj20030484

Published: 01 August 2003

...Alistair T. R. SIM; Monique L. BALDWIN; John A. P. ROSTAS; Jeff HOLST; Russell I. LUDOWYKE Modulation of exocytosis is integral to the regulation of cellular signalling, and a variety of disorders (such as epilepsy, hypertension, diabetes and asthma) are closely associated with pathological...

View articletitled, The role of serine/threonine protein phosphatases in exocytosis

Includes: Multimedia, Supplementary data

Articles

Endogenous phospholipase D2 localizes to the plasma membrane of RBL-2H3 mast cells and can be distinguished from ADP ribosylation factor-stimulated phospholipase D1 activity by its specific sensitivity to oleic acid

Elisabeth SARRI, Raul PARDO, Amanda FENSOME-GREEN, Shamshad COCKCROFT

Biochem J (2003) 369 (2): 319–329.

https://doi.org/10.1042/bj20021347

Published: 15 January 2003

... stimulated by many cell surface receptors that catalyses the hydrolysis of phosphatidylcholine (PC) to generate the second messenger phosphatidate and choline (Cho) [1 3]. Phosphatidate has been implicated in many cellular functions, including exocytosis, endocytosis, remodelling of the actin cytoskeleton...

View articletitled, Endogenous phospholipase D2 localizes to the plasma membrane of RBL-2H3 mast cells and can be distinguished from ADP ribosylation factor-stimulated phospholipase D1 activity by its specific sensitivity to oleic acid

Articles

The role of myosin in vesicle transport during bovine chromaffin cell secretion

Patricia ÑECO, Anabel GIL, María del MAR FRANCÉS, Salvador VINIEGRA, Luis M. GUTIÉRREZ

Biochem J (2002) 368 (2): 405–413.

https://doi.org/10.1042/bj20021090

Published: 01 December 2002

...Patricia ÑECO; Anabel GIL; María del MAR FRANCÉS; Salvador VINIEGRA; Luis M. GUTIÉRREZ Bovine adrenomedullary cells in culture have been used to study the role of myosin in vesicle transport during exocytosis. Amperometric determination of calcium-dependent catecholamine release from individual...

View articletitled, The role of myosin in vesicle transport during bovine chromaffin cell secretion

Includes: Multimedia, Supplementary data

Articles

Phosphorylation of SNAP-25 on serine-187 is induced by secretagogues in insulin-secreting cells, but is not correlated with insulin secretion

Carmen GONELLE-GISPERT, Maria COSTA, Masami TAKAHASHI, Karin SADOUL, Philippe HALBAN

Biochem J (2002) 368 (1): 223–232.

https://doi.org/10.1042/bj20020896

Published: 15 November 2002

... The Biochemical Society, London ©2002 2002 exocytosis islets phosphorylation protein kinase C soluble NSF ( N -ethylmaleimide-sensitive fusion protein)-attachment protein receptor (SNARE) Abbreviations used: BIS, bisindolylmaleimide I; BoNT, botulinum neurotoxin; CaMKII, Ca 2+ /calmodulin...

View articletitled, Phosphorylation of SNAP-25 on serine-187 is induced by secretagogues in insulin-secreting cells, but is not correlated with insulin secretion

Articles

Role of synaptotagmin in Ca2+-triggered exocytosis

Ward C. TUCKER, Edwin R. CHAPMAN

Biochem J (2002) 366 (1): 1–13.

https://doi.org/10.1042/bj20020776

Published: 15 August 2002

...Ward C. TUCKER; Edwin R. CHAPMAN The Ca 2+ -binding synaptic-vesicle protein synaptotagmin I has attracted considerable interest as a potential Ca 2+ sensor that regulates exocytosis from neurons and neuroendocrine cells. Recent studies have shed new light on the structure, biochemical/biophysical...

View articletitled, Role of synaptotagmin in Ca2+-triggered exocytosis

Articles

Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human

Mitsunori FUKUDA, Yukie OGATA, Chika SAEGUSA, Eiko KANNO, Katsuhiko MIKOSHIBA

Biochem J (2002) 365 (1): 173–180.

https://doi.org/10.1042/bj20011877

Published: 01 July 2002

...Mitsunori FUKUDA; Yukie OGATA; Chika SAEGUSA; Eiko KANNO; Katsuhiko MIKOSHIBA Synaptotagmin VII (Syt VII) has been proposed to regulate several different types of Ca 2+ -dependent exocytosis, but its subcellular localization (lysosome or plasma membrane) and the number of alternative splicing...

View articletitled, Alternative splicing isoforms of synaptotagmin VII in the mouse, rat and human

Articles

Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I

Gareth J.O. EVANS, Alan MORGAN

Biochem J (2002) 364 (2): 343–347.

https://doi.org/10.1042/bj20020123

Published: 01 June 2002

...Gareth J.O. EVANS; Alan MORGAN The secretory vesicle cysteine string proteins (CSPs) are members of the DnaJ family of chaperones, and function at late stages of Ca 2+ -regulated exocytosis by an unknown mechanism. To determine novel binding partners of CSPs, we employed a pull-down strategy from...

View articletitled, Phosphorylation-dependent interaction of the synaptic vesicle proteins cysteine string protein and synaptotagmin I

Articles

The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells

Thierry COPPOLA, Véronique PERRET-MENOUD, Sonia GATTESCO, Sarah MAGNIN, Isabel POMBO, Ulrich BLANK, Romano REGAZZI

Biochem J (2002) 362 (2): 273–279.

https://doi.org/10.1042/bj3620273

Published: 22 February 2002

...Thierry COPPOLA; Véronique PERRET-MENOUD; Sonia GATTESCO; Sarah MAGNIN; Isabel POMBO; Ulrich BLANK; Romano REGAZZI Rab3 GTPases regulate exocytosis of neurons, endocrine and exocrine cells. In the present paper, we report a system to measure the guanine nucleotide status of Rab3 proteins in living...

View articletitled, The death domain of Rab3 guanine nucleotide exchange protein in GDP/GTP exchange activity in living cells

Articles

Cholesterol-dependent interaction of syncollin with the membrane of the pancreatic zymogen granule

Alois HODEL, Seong J. AN, Neal J. HANSEN, Jared LAWRENCE, Barbara WÄSLE, Michael SCHRADER, J. Michael EDWARDSON

Biochem J (2001) 356 (3): 843–850.

https://doi.org/10.1042/bj3560843

Published: 08 June 2001

... subunits, and its association with the membrane may be stabilized by electrostatic interactions with either other proteins or phospholipids. 12 12 2000 23 3 2001 10 4 2001 The Biochemical Society, London ©2001 2001 exocrine pancreas exocytosis secretion secretory granule...

View articletitled, Cholesterol-dependent interaction of syncollin with the membrane of the pancreatic zymogen granule

Articles

Phosphatidylinositol transfer proteins and protein kinase C make separate but non-interacting contributions to the phosphorylation state necessary for secretory competence in rat mast cells

Jef A. PINXTEREN, Bastien D. GOMPERTS, Danise ROGERS, Scott E. PHILLIPS, Peter E. R. TATHAM, Geraint M. H. THOMAS

Biochem J (2001) 356 (1): 287–296.

https://doi.org/10.1042/bj3560287

Published: 08 May 2001

...Jef A. PINXTEREN; Bastien D. GOMPERTS; Danise ROGERS; Scott E. PHILLIPS; Peter E. R. TATHAM; Geraint M. H. THOMAS Mast cells permeabilized by streptolysin O undergo exocytosis when stimulated with Ca 2+ and guanosine 5′-[γ-thio]triphosphate but become progressively refractory to this stimulus...

View articletitled, Phosphatidylinositol transfer proteins and protein kinase C make separate but non-interacting contributions to the phosphorylation state necessary for secretory competence in rat mast cells

Articles

GLUT4 vesicle trafficking in rat adipocytes after ethanol feeding: regulation by heterotrimeric G-proteins

Laurie A. POIRIER, Nadia RACHDAOUI, Laura E. NAGY

Biochem J (2001) 354 (2): 323–330.

https://doi.org/10.1042/bj3540323

Published: 22 February 2001

... correspondence should be addressed (e-mail [email protected] ). 28 6 2000 6 11 2000 12 12 2000 The Biochemical Society, London © 2001 2001 adenosine exocytosis glucose transport insulin Biochem. J. (2001) 354, 323 330 (Printed in Great Britain) 323 GLUT4 vesicle trafficking...

View articletitled, GLUT4 vesicle trafficking in rat adipocytes after ethanol feeding: regulation by heterotrimeric G-proteins

Articles

Role of cytosolic phospholipase A2 in the production of lipid mediators and histamine release in mouse bone-marrow-derived mast cells

Noriaki NAKATANI, Naonori UOZUMI, Kazuhiko KUME, Makoto MURAKAMI, Ichiro KUDO, Takao SHIMIZU

Biochem J (2000) 352 (2): 311–317.

https://doi.org/10.1042/bj3520311

Published: 24 November 2000

... mediators, explaining the in vivo findings, the present study proposes a novel link between cPLA 2 and the degranulation machinery. anaphylaxis exocytosis leukotrienes prostaglandins Biochem. J. (2000) 352, 311 317 (Printed in Great Britain) 311 Role of cytosolic phospholipase A2 in the production...

View articletitled, Role of cytosolic phospholipase A2 in the production of lipid mediators and histamine release in mouse bone-marrow-derived mast cells

Articles

Changes in cytoplasmic calcium determine the secretory response to extracellular cations in human parathyroid cells: a confocal microscopy study using FM1-43 dye

Radu MIHAI, Teresa LAI, George J. SCHOFIELD, John R. FARNDON

Biochem J (2000) 352 (2): 353–361.

https://doi.org/10.1042/bj3520353

Published: 24 November 2000

[email protected] ). 9 5 2000 8 8 2000 15 9 2000 The Biochemical Society, London © 2000 2000 Calcium receptor (CaR) exocytosis endocytosis hyperparathyroidism rMTC6-23 cells Biochem. J. (2000) 352, 353 361 (Printed in Great Britain) 353 Changes in cytoplasmic calcium determine...

View articletitled, Changes in cytoplasmic calcium determine the secretory response to extracellular cations in human parathyroid cells: a confocal microscopy study using FM1-43 dye

Articles

Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Rory R. DUNCAN, David K. APPS, Michele P. LEARMONTH, Michael J. SHIPSTON, Robert H. CHOW

Biochem J (2000) 351 (1): 33–37.

https://doi.org/10.1042/bj3510033

Published: 26 September 2000

... of regulated exocytosis. Extensive reverse transcription–PCR, Northern blot analyses and in vitro translation reactions have been combined with immunological studies to provide data to suggest that neither DOC2α nor DOC2β is expressed at detectable levels in bovine adrenal chromaffin cells, and that a widely...

View articletitled, Is double C2 protein (DOC2) expressed in bovine adrenal medulla? A commercial anti-DOC2 monoclonal antibody recognizes a major bovine mitochondrial antigen

Articles

Sac phosphatase domain proteins

William E. HUGHES, Frank T. COOKE, Peter J. PARKER

Biochem J (2000) 350 (2): 337–352.

https://doi.org/10.1042/bj3500337

Published: 23 August 2000

...William E. HUGHES; Frank T. COOKE; Peter J. PARKER Advances in our understanding of the roles of phosphatidylinositol phosphates in controlling cellular functions such as endocytosis, exocytosis and the actin cytoskeleton have included new insights into the phosphatases that are responsible...

View articletitled, Sac phosphatase domain proteins

Articles

GLUT4 trafficking in insulin-stimulated rat adipose cells: evidence that heterotrimeric GTP-binding proteins regulate the fusion of docked GLUT4-containing vesicles

Cynthia M. FERRARA, Samuel W. CUSHMAN

Biochem J (1999) 343 (3): 571–577.

https://doi.org/10.1042/bj3430571

Published: 25 October 1999

... to photolabel and the increase in glucose transport activity. These results suggest that R s G s and R i G i modulate insulin-stimulated glucose transport by influencing the extent to which GLUT4 is associated with occluded vesicles attached to the plasma membrane during exocytosis, perhaps by regulating...

View articletitled, GLUT4 trafficking in insulin-stimulated rat adipose cells: evidence that heterotrimeric GTP-binding proteins regulate the fusion of docked GLUT4-containing vesicles

Articles

Inhibition of insulin-stimulated phosphorylation of the intracellular domain of phospholemman decreases insulin-dependent GLUT4 translocation in streptolysin-O-permeabilized adipocytes

Otto WALAAS, Robert S. HORN, S. Ivar WALAAS

Biochem J (1999) 343 (1): 151–157.

https://doi.org/10.1042/bj3430151

Published: 24 September 1999

... translocation of GLUT4 to the plasma membrane. 1 Deceased. 2 To whom correspondence should be addressed (e-mail [email protected] ). 22 1 1999 28 6 1999 15 7 1999 The Biochemical Society, London © 1999 1999 exocytosis fat cells glucose transport protein...

View articletitled, Inhibition of insulin-stimulated phosphorylation of the intracellular domain of phospholemman decreases insulin-dependent GLUT4 translocation in streptolysin-O-permeabilized adipocytes

Articles

High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Rory R. DUNCAN, Andrew C. DON-WAUCHOPE, Sompol TAPECHUM, Michael J. SHIPSTON, Robert H. CHOW, Peter ESTIBEIRO

Biochem J (1999) 342 (3): 497–501.

https://doi.org/10.1042/bj3420497

Published: 05 September 1999

...Rory R. DUNCAN; Andrew C. DON-WAUCHOPE; Sompol TAPECHUM; Michael J. SHIPSTON; Robert H. CHOW; Peter ESTIBEIRO Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique...

View articletitled, High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

Articles

nSec-1 (munc-18) interacts with both primed and unprimed syntaxin 1A and associates in a dimeric complex on adrenal chromaffin granules

Lee P. HAYNES, Alan MORGAN, Robert D. BURGOYNE

Biochem J (1999) 342 (3): 707–714.

https://doi.org/10.1042/bj3420707

Published: 05 September 1999

...Lee P. HAYNES; Alan MORGAN; Robert D. BURGOYNE The target-SNARE syntaxin 1A is an essential component of the core machinery required for regulated exocytosis (where SNARE is the soluble N -ethylmaleimide-sensitive fusion protein-attachment protein receptor). Syntaxin 1A interacts with a variety...

View articletitled, nSec-1 (munc-18) interacts with both primed and unprimed syntaxin 1A and associates in a dimeric complex on adrenal chromaffin granules

Articles

Rab6 is phosphorylated in thrombin-activated platelets by a protein kinase C-dependent mechanism: effects on GTP/GDP binding and cellular distribution

Michael L. FITZGERALD, Guy L. REED

Biochem J (1999) 342 (2): 353–360.

https://doi.org/10.1042/bj3420353

Published: 24 August 1999

...Michael L. FITZGERALD; Guy L. REED In platelets and other secretory cells, protein kinase C (PKC) plays a role in exocytosis stimulated by physiological extracellular signals, although its linkage to the secretory machinery is poorly understood. We investigated whether Rab6, a GTP-binding protein...

View articletitled, Rab6 is phosphorylated in thrombin-activated platelets by a protein kinase C-dependent mechanism: effects on GTP/GDP binding and cellular distribution

Articles

Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells

Nathalie CHARVIN, Geoff WILLIAMS, Robert D. BURGOYNE

Biochem J (1999) 341 (1): 179–183.

https://doi.org/10.1042/bj3410179

Published: 24 June 1999

...Nathalie CHARVIN; Geoff WILLIAMS; Robert D. BURGOYNE Doc2 is a C2-domain-containing protein that is highly expressed in the nervous system and has a constitutively expressed isoform. It has been implicated as a potential Ca 2+ sensor in regulated exocytosis, and has been suggested to be associated...

View articletitled, Doc2 is not associated with known regulated exocytotic or endosomal compartments in adrenal chromaffin cells

Articles

SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion

Carmen GONELLE-GISPERT, Philippe A. HALBAN, Heiner NIEMANN, Michael PALMER, Stefan CATSICAS, Karin SADOUL

Biochem J (1999) 339 (1): 159–165.

https://doi.org/10.1042/bj3390159

Published: 25 March 1999

... 1999 exocytosis islet neurotoxin regulated release SNARE Biochem. J. (1999) 339, 159 165 (Printed in Great Britain) 159 SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion Carmen GONELLE-GISPERT*, Philippe A. HALBAN*, Heiner NIEMANN...

View articletitled, SNAP-25a and -25b isoforms are both expressed in insulin-secreting cells and can function in insulin secretion

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