..., biosynthesis of proline and polyamines, and regulation of intracellular signaling pathways. Arginine biosynthesis and catabolism have been linked to TGF-β-induced activation of fibroblasts in the context of pulmonary fibrosis; however, a thorough study on the metabolic and signaling roles of arginine...

.... We examined the spatial restriction of Ca 2+ release through the inositol 1,4,5-triphosphate receptor 1 (IP 3 R1) in response to interleukin-1 (IL-1) and the functions of the adhesion-associated proteins, focal adhesion kinase (FAK) and protein tyrosine phosphatase-α (PTPα). In cultured fibroblasts...

... the complexity of the human context. To further overcome these limitations, we have acquired dermal fibroblasts from two ODDD-affected individuals harbouring D3N and V216L mutations in Cx43, along with familial controls. Using these ODDD patient dermal fibroblasts, which naturally produce less GJA1 gene product...

... to their functions as Ras-exchange factors, Ras-GRF1 and -GRF2 may act as adaptors that bind PLCγ1 and restrict Ca 2+ signalling to the vicinity of focal adhesions, indicating a new role for these GRFs that is required for IL-1 induction of the Ras→ERK pathway and MMP-3 expression. fibroblast inositol 1,4,5...

...) which activate intracellular signalling pathways. In the present paper we report that uPA-mediated cell migration requires an interaction with fibulin-5. uPA stimulates migration of wild-type MEFs (mouse embryonic fibroblasts) ( Fbln5 +/+ MEFs), but has no effect on fibulin-5-deficient ( Fbln5...

...Hiroaki Nakajima; Yoshiyuki Ezaki; Tomoyashu Nagai; Ryosuke Yoshioka; Genji Imokawa We recently reported that overexpression of the elastase NEP (neutral endopeptidase) by fibroblasts plays a pivotal role in the mechanism of UVB-induced skin wrinkling by degrading dermal elastic fibres. Since UVB...

... in a progressive accumulation of scarring proteins (fibrosis) that, with increasing severity, alters tissue structure and function, leading to cirrhosis and liver failure. Efforts to modulate the fibrogenesis process have focused on understanding the biology of the heterogeneous liver fibroblast populations...

...Xia Liu; Ping Li; Ping Liu; Renping Xiong; En Zhang; Xingyun Chen; Dayong Gu; Yan Zhao; Zhengguo Wang; Yuanguo Zhou The bi-directional regulation of TGF-β1 (transforming growth factor-β1) on fibroblast proliferation with stimulation at low concentration, but inhibition at high concentration, has...

...Adrien Rossary; Khelifa Arab; Jean-Paul Steghens The strong ROS (reactive oxygen species) production, part of an antioxidant response of human fibroblasts triggered by DHA (docosahexaenoic acid; C 22:6 , n −3 ), served as a model for deciphering the relative contribution of NOX (NADPH oxidase...

...Jennifer R. Mackley; Joji Ando; Pawel Herzyk; Steven J. Winder Primary fibroblasts isolated from foetal mouse cornea, skin and tendon were subjected to linear shear stress and analysed for morphological parameters and by microarray, as compared with unstimulated controls. Approx. 350 genes were...

... pathways and production of MMPs (matrix metalloproteases) and chemokines. Stimulation of human skin fibroblasts or articular chondrocytes with IL-1 or TNF-α in the presence of HC-gp39 resulted in a marked reduction of both p38 mitogen-activated protein kinase and stress-activated protein kinase/Jun N...

...Daniela KESSLER-BECKER; Thomas KRIEG; Beate ECKES In vivo , fibroblasts reside in connective tissues, with which they communicate in a reciprocal way. Such cell–extracellular matrix interactions can be studied in vitro by seeding fibroblasts in collagen lattices. Depending upon the mechanical...

... in hepatocytes, macrophages and astrocytes. In the present study, we identify nuclear and cytosolic pools of apoE in human fibroblasts. Fibroblast apoE mRNA and protein levels were up-regulated during staurosporine-induced apoptosis and this was correlated with increased caspase-3 activity and apoptotic...

.... These findings suggest that metabolic alterations are involved in tumourigenesis and tumour suppression; however, little is known about the metabolic pathways that contribute to these processes. Using the human fibroblast model of in vitro senescence, we analysed age-dependent changes in the cellular...

... bp exon 3, which results in a premature stop codon. Quantification of the two mRNA species in different cell types (blood-derived cells, fibroblasts and endothelial cells) and cell lines showed that the amount of exon-3-containing mRNA is correlated closely to PTPS activity. The ratio of exon-3...

...Jeremy P. E. SPENCER; Gunter G. C. KUHNLE; Robert J. WILLIAMS; Catherine RICE-EVANS Understanding the cellular effects of flavonoid metabolites is important for predicting which dietary flavonoids might be most beneficial in vivo . Here we investigate the bioactivity in dermal fibroblasts...

.... By cell fractionation, we demonstrate that, in addition to a previously reported minute amount of acidic sphingomyelinase activity, a substantial amount of neutral sphingomyelinase activity resides in caveolae of human skin fibroblasts. This caveolar neutral sphingomyelinase activity was also detected...

... to activate latent TGF-β1. Any TSP-mediated activation of TGF-β1 must depend on additional factor(s) not present in our systems. cytokine fibroblast platelet wound healing Biochem. J. (2000) 350, 291 298 (Printed in Great Britain) 291 Thrombospondin 1 does not activate transforming growth factor b1...