... have investigated the interaction of four different RGD-binding integrins (α5β1, αVβ1, αVβ3 and αVβ6) with fibronectin (FN) using surface plasmon resonance. The ability of inhibitors to reverse ligand binding was assessed by their capacity to increase the dissociation rate of pre-formed integrin–FN...

... cell attachment to fibronectin recruits MEKK2 to focal adhesion complexes, and that MEKK2 knockdown is associated with stabilized focal adhesions and significant inhibition of tumour cell migration. In the present study we investigate MEKK2 function in focal adhesions and we report that MEKK2...

...Christine Y. Chuang; Georg Degendorfer; Astrid Hammer; John M. Whitelock; Ernst Malle; Michael J. Davies ECM (extracellular matrix) materials, such as laminin, perlecan, type IV collagen and fibronectin, play a key role in determining the structure of the arterial wall and the properties of cells...

... into microfibrils and their homoeostasis is poorly understood and is often compromised in connective tissue disorders such as Marfan syndrome and other fibrillinopathies. Using interaction mapping studies, we demonstrate that fibrillins require the complete gelatin-binding region of fibronectin for interaction...

...Montserrat Porta-de-la-Riva; Jelena Stanisavljevic; Josue Curto; Clara Francí; Víctor Manuel Díaz; Antonio García de Herreros; Josep Baulida Fibronectins are cell-secreted glycoproteins that modulate cell attachment, spreading, migration, morphology, differentiation and oncogenic transformation...

... (fibronectin type III) domains or one REJ (receptor of egg jelly protein) module in the same portion of polypeptide. Stimulated by recent atomic force microscopy work, we re-examined the similarity of these four domains with a FNIII sequence profile showing the evolutionary relationship. Two of the predicted...

...A. Paul Mould; Ewa J. Koper; Adam Byron; Grit Zahn; Martin J. Humphries Integrin α5β1 is a key receptor for the extracellular matrix protein fibronectin. Antagonists of human integrin α5β1 have therapeutic potential as anti-angiogenic agents in cancer and diseases of the eye. However, the structure...

... nephropathy fibronectin G-protein-coupled receptor (GPCR) phosphoinositide 3-kinase (PI3K) type IV collagen The kidney is a major target organ of diabetic complications. Mesangial expansion with matrix accumulation is the pathological hallmark of diabetic nephropathy [ 1 ]. Hyperglycaemia...

... and consequent cell proliferation, but decreased cell adhesion to fibronectin and collagens I and IV, compared with control cells. When triggered by laminins, NEU3 clearly stimulated phosphorylation of FAK (focal adhesion kinase) and ERK (extracellular-signal-regulated kinase), whereas there was no activation...

... in vitro with a number of extracellular matrix proteins, e.g. Fn (fibronectin). In view of its strong binding to Fn, we examined in the present study whether the released soluble ectodomain can bind to the fibrillar Fn matrix under cell-culture conditions and, if so, influence its assembly. In this study...

...Baljit WALIA; Florencia E. CASTANEDA; Lixin WANG; Vasantha L. KOLACHALA; Rahul BAJAJ; Jesse ROMAN; Didier MERLIN; Andrew T. GEWIRTZ; Shanthi V. SITARAMAN Fibronectin (FN) is a multifunctional protein that plays important roles in many biological processes including cell adhesion and migration...

... compatible with tryptase monomers in complex with heparin. The monomers were, in contrast to tryptase in the tetrameric form, inhibited by bovine pancreatic trypsin inhibitor. Further, the monomers, but not the tetramers, degraded fibronectin. Formation of active monomers was more pronounced at pH7.5 than...

... b $ and TGFb, which may have implications in cancer and a number of inflammatory and fibrotic diseases where expression of both proteins has been documented. Key words: adhesion, binding, cell, fibronectin, vitronectin. (MMP-2 and MMP-9) [9] and cathepsin [10], which elicit effects by proteolytic...

... and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K D of about 3×10 -8 M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1—nidogen-1...

... of triglyceride accumulation. Inhibitor treatment prevented the fibronectin-network degradation, as well as the induction of the genes for peroxisome-proliferator-activated receptor γ and adipsin, two adipocyte phenotype markers. Inhibitor treatment was effective when applied during the early stages of adipocytic...

...Jungyean KIM; Innoc HAN; Yeonhee KIM; Seungin KIM; Eok-Soo OH Fibronectin (FN) stimulates multiple signalling events including mitogen-activated protein kinase (MAPK) activation. During cell spreading, both the cell-binding domain and the C-terminal heparin-binding domain (HepII) of FN co...

... days. CTGF protein is also present in glomeruli of human patients with diabetic nephropathy. No CTGF was detected in either normal murine or human glomeruli. Transient transfection of a transformed human mesangial cell line with a CTGF–V5 epitope fusion protein markedly increased fibronectin...

...Mariko NAITO; Tomohiko FUKUDA; Kiyotoshi SEKIGUCHI; Takeshi YAMADA We have recently shown that α antigen (α-Ag), the immunodominant antigen of mycobacteria, has a novel fibronectin (FN)-binding motif that is unique among mycobacteria [Naito, Ohara, Matsumoto and Yamada (1998) J. Biol. Chem. 273...