... understood. In the present study, we investigated the interplay between intracellular cAMP and Ca 2+ concentrations ([cAMP] i and [Ca 2+ ] i respectively) in the pancreatic β-cell line MIN6 using total internal reflection fluorescence microscopy. For measuring [cAMP] i , we developed a genetically encoded...

..., fluorescent probes may display different organizing behaviour from their corresponding endogenous lipids. A full characterization of these probes is therefore required for proper interpretation of fluorescence microscopy data in complex membrane systems. Model membrane studies provide essential clues...

... (CPP) cholesterol endocytosis fluorescence microscopy methyl-β-cyclodextrin (MβCD) octa-arginine CPPs (cell-penetrating peptides) or protein-transduction domains have now been described for two decades [ 1 , 2 ]. During this period, several different CPP classes have been described...

... compilation, The Biochemical Society, London 2007 cell-penetrating peptide endocytosis fluorescence microscopy KG1a cell line leukaemia octa-arginine The scientific literature describes an ever-increasing library of peptides that mediate the lysis of biological membranes or have capacities...

... and iron chelation in single, intact cells was recorded on-line by using digital fluorescence microscopy. Intracellular concentrations of various fluorescent iron chelators were determined by using a spectrofluorophotometer subsequent to lysis of probe-loaded cells and were found to depend...

... fluorescence, confocal and electron microscopy. The peptide previously isolated from skin secretions of the frog Rana temporaria has the sequence FVQWFSKFLGRIL-NH 2 . With regard to fluorescence microscopy, we applied, for the first time, a triple-staining method based on the fluorochromes 5-cyano-2,3-ditolyl...

... 2004 confocal laser-scanning microscopy fluorescence microscopy enhanced green fluorescent protein (EGFP) self-assembly S-layer fusion protein S-liposome Abbreviations used: DMEM, Dulbecco's modified Eagle's medium; DPPC, dipalmitylphosphatidylcholine; DTT, dithiothreitol; EGFP...

.... Both the activity and expression of MnSOD mRNA were enhanced in affected fibroblasts. Confocal fluorescence microscopy and immunohistochemical studies revealed the presence of MnSOD in mitochondria of CLN6 fibroblasts and in CLN6 brain sections within both neurons and hypertrophic astrocytes...