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1-8 of 8
Keywords: fluorescence resonance energy transfer
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Articles
Quantitative live-cell imaging of GPCR downstream signaling dynamics
Available to PurchaseIn Collection
Towards systems biochemistry
Journal:
Biochemical Journal
Biochem J (2022) 479 (8): 883–900.
Published: 21 April 2022
... on behalf of the Biochemical Society 2022 dopamine fluorescence resonance energy transfer G-protein-coupled receptors serotonin G-protein-coupled receptors (GPCRs), the largest family of membrane proteins, are triggered by various types of ligands, such as neurotransmitters (e.g. dopamine...
Includes: Supplementary data
Articles
Excitation energy transfer kinetics of trimeric, monomeric and subunit-depleted Photosystem I from Synechocystis PCC 6803
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2021) 478 (7): 1333–1346.
Published: 06 April 2021
... 3 2021 9 3 2021 9 3 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 circular dichroism cyanobacteria fluorescence resonance energy transfer photosynthesis time-resolved spectroscopy Photosystem I (PSI...
Includes: Supplementary data
Articles
Kringles of substrate plasminogen provide a ‘catalytic switch' in plasminogen to plasmin turnover by Streptokinase
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2020) 477 (5): 953–970.
Published: 06 March 2020
...Vandna Sharma; Shekhar Kumar; Girish Sahni To understand the role of substrate plasminogen kringles in its differential catalytic processing by the streptokinase — human plasmin (SK-HPN) activator enzyme, Fluorescence Resonance Energy Transfer (FRET) model was generated between the donor labeled...
Articles
The antifungal peptide CGA-N12 inhibits cell wall synthesis of Candida tropicalis by interacting with KRE9
Open Access
Journal:
Biochemical Journal
Biochem J (2020) 477 (3): 747–762.
Published: 14 February 2020
... forces. The results of the fluorescence resonance energy transfer (FRET) assay showed that the distance between CGA-N12 and KRE9 was 7 ∼ 10 nm during their interaction. Therefore, we concluded that the target of CGA-N12 in the C. tropicalis cell membrane is KRE9, and that CGA-N12 weakly binds to KRE9...
Includes: Supplementary data
Articles
Quaternary structures of opsin in live cells revealed by FRET spectrometry
Available to PurchaseAshish K. Mishra, Megan Gragg, Michael R. Stoneman, Gabriel Biener, Julie A. Oliver, Przemyslaw Miszta, Slawomir Filipek, Valerică Raicu, Paul S.-H. Park
Journal:
Biochemical Journal
Biochem J (2016) 473 (21): 3819–3836.
Published: 27 October 2016
... ( [email protected] ) or Paul S.-H. Park ( [email protected] ) 5 5 2016 25 8 2016 12 9 2016 13 9 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 fluorescence resonance energy transfer G-protein-coupled receptors...
Includes: Supplementary data
Articles
Enhancement of correct protein folding in vivo by a non-lytic baculovirus
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2004) 382 (2): 695–702.
Published: 24 August 2004
... further using a novel FRET (fluorescence resonance energy transfer)-based assay. To achieve this, we constructed a concatenated fusion protein comprising LUC (luciferase) sandwiched between EYFP (enhanced yellow fluorescent protein) and ECFP (enhanced cyan fluorescent protein). The distance separating...
Articles
Secondary-structure characterization by far-UV CD of highly purified uncoupling protein 1 expressed in yeast
Available to PurchasePierre DOUETTE, Rachel NAVET, Fabrice BOUILLENNE, Alain BRANS, Claudine SLUSE-GOFFART, André MATAGNE, Francis E. SLUSE
Journal:
Biochemical Journal
Biochem J (2004) 380 (1): 139–145.
Published: 15 May 2004
... C-terminus in Saccharomyces cerevisiae mitochondria. The recombinant-tagged UCP1 was purified by immobilized metal-ion affinity chromatography to homogeneity (>95%). This made it suitable for subsequent biophysical characterization. Fluorescence resonance energy transfer experiments showed that n...
Articles
Conformational changes in activated protein C caused by binding of the first epidermal growth factor-like module of protein S
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2000) 349 (3): 757–764.
Published: 25 July 2000
..., electrospray ionization MS; Fl-FPR-APC, fluorescein-acetamido-D-phenylalanyl-prolyl- arginyl-APC; FRET, fluorescence resonance energy transfer ; OR, octadecylrhodamine ; PC, dioleoylphosphatidylcholine ; PS, dioleoylphosphatidyl- serine. 1 To whom correspondence should be addressed at University Maastricht (e...
