... and the Biochemical Society under a transformative agreement with JISC. antibodies edge-shift fluorescence spectroscopy protein dynamics protein stability Maintenance of function and hence efficacy is an important consideration for the developability of biomolecules. This is driven in part...

... To whom correspondence should be addressed (email [email protected] ). 22 4 2010 8 7 2010 15 7 2010 15 7 2010 fibrillation fluorescence spectroscopy insulin temperature dependence thioflavin T (ThT) zinc Insulin, a peptide hormone crucial for glucose metabolism...

... [email protected] ). 20 7 2009 15 9 2009 21 9 2009 21 9 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 ethylene insensitive 2 (EIN2) ethylene signalling fluorescence spectroscopy membrane protein plant hormone protein–protein...

... alcohol-binding site alcoholism fluorescence spectroscopy mass spectrometry (MS) photoaffinity protein kinase Cϵ (PKCϵ) Alcoholism and alcohol abuse causes major health problems worldwide. Alcohol is casually related to more than 60 different medical conditions. Overall 4% of the global...

... fluorescence spectroscopy thermodynamics The human cytomegalovirus is a DNA virus that can cause a variety of important diseases in immunocompromised patients and in newborns [ 1 ]. These include gastrointestinal diseases, pneumonia and retinitis, whereas newborns born with cytomegalic inclusion...

.... Fluorescence spectroscopy and CD studies were used to directly monitor the binding of decavanadate to the enzyme. Moreover, competition assays show that decavanadate is a potent non-competitive inhibitor of the phosphohydrolase activity, and mutagenesis studies indicate that the binding of decavanadate does...

... p110α/p85α PI3K in a GTP-dependent manner. Activated Gα q binds to the p110α/p85α PI3K with an apparent affinity that is seven times stronger than that for Gα q ·GDP as measured by fluorescence spectroscopy. In contrast, Gα q did not bind to the p110γ PI3K. Fluorescence spectroscopy experiments also...

... 7 4 2005 The Biochemical Society, London 2005 annexin A13 calcium binding CD spectroscopy fluorescence spectroscopy phospholipid binding thermal stability The annexin superfamily comprises a group of calcium- and phospholipid-binding proteins characterized by a common...

... in nucleotide excision repair remains elusive. In the present study, we have systematically examined the binding of RPA to a battery of well-defined ssDNA (single-stranded DNA) substrates using fluorescence spectroscopy. These substrates contain adducts of (6-4) photoproducts, N -acetyl-2-aminofluorene-, 1...

... binding to RNA is clearly dominated by a favourable entropic effect ( T Δ S =20.5 kJ/mol), with a minor contribution from a favourable enthalpy change (Δ H =−5.3 kJ/mol). Fluorescence spectroscopy also allowed us to evaluate the initial binding of GTP to such an enzyme, thereby separating the GTP binding...

... , and AB-R105A (Arg 105 →Ala) activates it prematurely. We used techniques of enzymology, surface plasmon resonance and fluorescence spectroscopy to analyse the defects in each mutant. AB-E104K was deficient in binding to AA, AB-T49K was deficient in binding to ADP and AB-R105A bound ADP exceptionally...

[email protected] ). 4 11 2003 2 12 2003 5 12 2003 5 12 2003 The Biochemical Society, London ©2004 2004 fluorescence spectroscopy GoLoco motif G-protein regulatory motif (GPR motif) guanine nucleotide dissociation inhibitor heterotrimeric G-protein surface plasmon resonance...

... reveals two main secondary-structure contributions, α-helix and random coil (approx. 30% each), corresponding mainly to the annexin C-terminal tetrad and the N-terminus respectively. On calcium binding, an increase in α-helix and a decrease in random coil are detected. Fluorescence spectroscopy reveals...

..., the sedimentation coefficient was increased from 9.0S to 9.8S. CD and fluorescence spectroscopy of HIR-MAED revealed only minor insulin-induced changes in the secondary structure. Similarity with wild-type receptors has also been demonstrated by the differential insertion of insulin-bound and free HIR-MAED...

... be addressed (e-mail [email protected] ). 8 12 1999 3 7 2000 24 7 2000 The Biochemical Society, London © 2000 2000 Ca2+ transport fluorescence spectroscopy membrane reconstitution proteinprotein interactions spin-label EPR Biochem. J. (2000) 351, 195 205 (Printed in Great...

... 27 8 1999 18 10 1999 The Biochemical Society, London © 2000 2000 fluorescence spectroscopy gel-filtration chromatography membrane protein folding membrane transport stilbene disulphonates Biochem. J. (2000) 345, 33 41 (Printed in Great Britain) 33 Mechanism of band 3 dimer...

... ). 18 9 1998 7 12 1998 20 1 1999 The Biochemical Society, London © 1999 1999 beta-oxidation fluorescence spectroscopy pyrenedodecanoic acid Biochem. J. (1999) 339, 193 199 (Printed in Great Britain) 193 High-affinity binding of very-long-chain fatty acyl-CoA esters...