..., including calcium regulation, cell survival, cell polarity, phase separation-mediated actin reorganization, and SD–focal adhesion dynamics. Collectively, our emerging understanding of the broader cellular context of nephrin signaling provides important insight for clinical strategies to mitigate podocyte...

... potential, and low numbers. The key transcription regulators of mitochondrial biogenesis also decreased. These results indicate that mitochondrial dysfunction leads to an inability to support the pluripotency maintenance. Moreover, down-regulated glutathione metabolism and up-regulated focal adhesion...

... adhesion, a critical step in the second phase of insulin secretion, we examined the effect of silencing SCGN on focal adhesion molecules, including FAK (focal adhesion kinase) and paxillin, and the cell survival molecules ERK1/2 (extracellular-signal-regulated kinase 1/2) and Akt. We found that glucose...

.... Cells expressing a mutant vinculin deficient in actin binding and bundling display migration and traction force defects. Vinculin binding to actin is critical for cell migration and force generation. actin adhesion focal adhesion force vinculin Cell migration is a complex process...

... cell attachment to fibronectin recruits MEKK2 to focal adhesion complexes, and that MEKK2 knockdown is associated with stabilized focal adhesions and significant inhibition of tumour cell migration. In the present study we investigate MEKK2 function in focal adhesions and we report that MEKK2...

... as part of a regulated, but continuous, process. A more dynamic situation may occur at FAs (focal adhesions), where a scaffolding AC could play a key regulatory and dynamic structural role. FAs are large macromolecular assemblies through which both mechanical force and regulatory signals are transmitted...

...Kentaro Kajiwara; Takayuki Yamada; Takeshi Bamba; Eiichiro Fukusaki; Fumio Imamoto; Masato Okada; Chitose Oneyama The proto-oncogenic tyrosine kinase c-Src is up-regulated in various human cancers, implicating its role in tumour progression. Upon activation, c-Src translocates to focal adhesions...

... as with focal adhesions and cell junctions, and are probably involved in cell migration, polarity and proliferation. Finally, we consider the association of GCKIIIs with a number of human diseases, particularly cerebral cavernous malformations. 1 Correspondence may be addressed to either of these authors...

..., although it is unclear as to the nature of the protrusions, or whether they involved active protein synthesis [ 18 ]. © The Authors Journal compilation © 2013 Biochemical Society 2013 cell division cycle 42 (Cdc42) focal adhesion mRNA Rac1 RhoA translation SV40 (simian virus 40...

... investigate the effect of the electrophilic prostaglandin 15d-PGJ 2 (15-deoxy-Δ 12,14 -prostaglandin J 2 ) on metastatic properties of breast cancer cells. 15d-PGJ 2 was shown to decrease migration, stimulate focal-adhesion disassembly and cause extensive F-actin (filamentous actin) reorganization at low...

... with vinculin in vivo , and this association is promoted by PtdIns(4,5) P 2 . SNTIII also specifically co-localizes with vinculin within focal adhesions when transiently expressed in mammalian cells. In contrast, other regions of synemin show distinct localization patterns in comparison with those of SNTIII...

... of the type IV intermediate filament protein synemin interacts directly with the focal adhesion protein vinculin in its active state. Interaction of adhesion components with intermediate filaments could serve as a general mechanism to regulate cell- and tissue-specific cytoskeleton-membrane attachment...

...: the potential tumour suppressor Tes, an actin-binding protein mainly located at focal adhesions; and EVL (Ena/vasodilator-stimulated phosphoprotein-like protein), another actin-binding protein, equally recruited at focal adhesions. Interactions between spectrin and overexpressed Tes and EVL were confirmed by co...

... 12 12 2003 The Biochemical Society, London ©2004 2004 α-actinin actin bundling cytoskeleton focal adhesion phosphoinositide protein flexibility Abbreviations used: CH, calponin homology; GST, glutathione S-transferase. Biochem. J. (2004) 378, 1067 1072 (Printed in Great...

... myogenic cells identified by bromodeoxyuridine and desmin double labelling in knockout mice on the first 2 days after injury. Expression of INTRODUCTION Focal adhesions are specialized cell-substratum junctions that are present in cells attaching to extracellular matrix. They are formed around...

...Huaiyang CHEN; Akiko ISHII; Wai-Keung WONG; Lan Bo CHEN; Su Hao LO Tensin is a focal-adhesion molecule that binds to actin filaments and interacts with phosphotyrosine-containing proteins. To analyse tensin's function in mammals, we have cloned tensin cDNAs from human and cow. The isolated approx...