... equivocal. By using diverse substrates and inhibitors and a kinase-deficient bisphosphatase-active variant of the bifunctional enzyme PFK2/FBP2 (6-phosphofructo-2-kinase–fructose-2,6-bisphosphatase), we demonstrate an essential role for fructose 2,6-bisphosphate in the induction of G6pc and other ChREBP...

... relative to the 18S RNA. The amplification of the correct PCR fragments was confirmed by DNA sequencing. β-cell fructose 2,6-bisphosphate glucokinase insulin islets 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase (PFK-2/FBPase-2) PFK-2/FBPase-2 (6-phosphofructo-2-kinase...

...Mark H. RIDER; Luc BERTRAND; Didier VERTOMMEN; Paul A. MICHELS; Guy G. ROUSSEAU; Louis HUE Fru-2,6-P 2 (fructose 2,6-bisphosphate) is a signal molecule that controls glycolysis. Since its discovery more than 20 years ago, inroads have been made towards the understanding of the structure–function...

...-aminoisobutyrate, ATP, glucose 6-phosphate (Glc6 P ) and fructose 2,6-bisphosphate [Fru(2,6) P 2 ], but maintain cell integrity in the presence of ATP as judged by the retention of cytoplasmic enzymes. During incubation with 25mM glucose, an ATP-generating system and saturating concentrations of Fru(2,6) P 2...

..., a single 57000- M r band was labelled on incubation of the dephosphorylated WISK preparation with PDK1 and [γ- 32 P]ATP. These findings provide evidence for the existence of a new protein kinase in the insulin signalling pathway, probably downstream of PDK1. fructose 2,6-bisphosphate glycolysis...