...Macarena Rodriguez-Walker; Aldo A. Vilcaes; Eduardo Garbarino-Pico; José L. Daniotti Gangliosides are sialic acid-containing glycosphingolipids mainly expressed at the outer leaflet of the plasma membrane. Sialidase NEU3 is a key enzyme in the catabolism of gangliosides with its up-regulation...

...Fernando M. Ruggiero; Aldo A. Vilcaes; Ramiro Iglesias-Bartolomé; José L. Daniotti ST3Gal-II, a type II transmembrane protein, is the main mammalian sialyltransferase responsible for GD1a and GT1b ganglioside biosynthesis in brain. It contains two putative N-glycosylation sites (Asn 92 and Asn 211...

...Kazunori Yamaguchi; Koichi Koseki; Momo Shiozaki; Yukiko Shimada; Tadashi Wada; Taeko Miyagi Gene expression of the human plasma membrane-associated sialidase (NEU3), a key enzyme for ganglioside degradation, is relatively high in brain and is modulated in response to many cellular processes...

... substrate specificity towards gangliosides. In this respect, sialidase NEU3 modulates cell-surface biological events and plays a pivotal role in different cellular processes, including cell adhesion, recognition and differentiation. At the moment, no detailed studies concerning the subcellular localization...

... of PC12 (pheochromocytoma) cells. Among the 22 hybrid clones, mAb PR#1 specifically detected a fucoganglioside Fuc(Gal)-G M1 [α-fucosyl(α-galactosyl)-G M1 ], a ganglioside homologous with G M1a (II 3 NeuAc,GgOse 4 Cer), as a novel member of microdomain components with biological functions. In the presence...

...Christiane Barbat; Maylis Trucy; Maurizio Sorice; Tina Garofalo; Valeria Manganelli; Alain Fischer; Fabienne Mazerolles We previously showed that the association of CD4 and G M3 ganglioside induced by CD4 ligand binding was required for the down-regulation of adhesion and that aggregation...

...Mike P. Williamson; Yu Suzuki; Nathan T. Bourne; Tetsuo Asakura Amyloid β-peptide (Aβ) is a major component of plaques in Alzheimer's disease, and formation of senile plaques has been suggested to originate from regions of neuronal membrane rich in gangliosides. Here we demonstrate using NMR on 15...

... in complex with a sialylated ligand, the ganglioside analogue DSLc4 [α(2,3)/α(2,6) disialyl lactotetraosyl 2-(trimethylsilyl)ethyl], which allows for a detailed description of the binding site, required for structure-guided inhibitor design. Mutagenesis and binding assays were used to demonstrate a key...

...-sensitive cell line. The ganglioside pattern in CABA I cells is more complex compared with that of CABA-PTX cells. Specifically, we found that the total ganglioside content in CABA-PTX cells was approximately half of that in CABA I cells, and GM3 ganglioside content was remarkably higher in the drug...

...Kengo Kato; Kiyoto Shiga; Kazunori Yamaguchi; Keiko Hata; Toshimitsu Kobayashi; Kaoru Miyazaki; Shigeru Saijo; Taeko Miyagi We have found previously that human plasma-membrane-associated sialidase (NEU3), a key glycosidase for ganglioside degradation, was markedly up-regulated in human colon...

...-sulphate proteoglycans, as well as generation of a negatively charged sialidase-sensitive intercellular environment between the stroma and the myeloid progenitors. In the present study, we have investigated the production, distribution and role of gangliosides in an experimental model of in vitro...

..., muscle and kidney contained both isoforms; liver showed the highest expression, and the short form was predominant in this organ. In transiently transfected COS-1 cells, enzyme activity was markedly increased with gangliosides as well as with glycoproteins and oligosaccharides as substrates compared...

... to diabetic nephropathy; however, involvement of HP in the regulation of mesangial cell growth or hypertrophy has been poorly studied. Although gangliosides are known to regulate cell proliferation, their potential role in mesangial cell-growth perturbations has hardly been explored. In the present study, we...

...Pilar M. CRESPO; Adolfo R. ZURITA; Claudio G. GIRAUDO; Hugo J. F. MACCIONI; Jose L. DANIOTTI GEM (glycosphingolipid-enriched microdomains) are specialized detergent-resistant domains of the plasma membrane in which some gangliosides concentrate. Although genesis of GEM is considered to occur...

... activities could not be ascribed to cross-contamination of MAM by Golgi membranes. Cer-Glc-T was found to have a marked preference for ceramide bearing phytosphingosine as sphingoid base. SAT activities in MAM led to the synthesis of G M3 ganglioside and small amounts of G D3 . G M1 was also synthesized...