... R184G failed to form functional GJs in DKO HEK293 cells with impaired localizations. These genetically engineered HEK293 cells are well suited for patch clamp study of human GJ channels. Cx43 gap junction channel HEK-293 cells patch clamp single channel analysis Gap junction (GJ) channels...

... ) 28 3 2023 29 6 2023 30 6 2023 © 2023 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2023 connexin mutants gap junction channel heterotypic gap junction patch clamp We conducted similar experiments on cell pairs with one...

... for comparison. ( C ) The average slope γ j s of the GJs of G8G46V53 as well as three individual variants are plotted as a bar graph. The statistical differences of each variant in comparison with γ j Cx50 GJ channel are shown (*** P  < 0.001). connexin50 gap junction channel [Mg 2+ ] i -dependent...

... to test heterotypic docking compatibility. Reproduced from [ 10 ]: Bai, D. and Wang, A.H. (2014) Extracellular domains play different roles in gap junction formation and docking compatibility. Biochem. J. 458 , 1–10. connexin26 connexin40 connexin43 docking mechanism gap junction channel...

...Donglin Bai; Ao Hong Wang GJ (gap junction) channels mediate direct intercellular communication and play an important role in many physiological processes. Six connexins oligomerize to form a hemichannel and two hemichannels dock together end-to-end to form a GJ channel. Connexin extracellular...