Page 1 | Search Results | Biochemical Journal

1-48 of 48

Sort by

Sort Order Select

Articles

PT-1 selectively activates AMPK-γ1 complexes in mouse skeletal muscle, but activates all three γ subunit complexes in cultured human cells by inhibiting the respiratory chain

Thomas E. Jensen, Fiona A. Ross, Maximilian Kleinert, Lykke Sylow, Jonas R. Knudsen, Graeme J. Gowans, D. Grahame Hardie, Erik A. Richter

Biochem J (2015) 467 (3): 461–472.

https://doi.org/10.1042/BJ20141142

Published: 17 April 2015

... 2 (ACC2), nor did it stimulate glucose transport. Surprisingly, however, in human embryonic kidney (HEK) 293 cells expressing human γ1, γ2 or γ3, PT-1 activated all three complexes equally. We were unable to reproduce previous findings suggesting that PT-1 activates AMPK by direct binding between...

View article titled, PT-1 selectively activates AMPK-γ1 complexes in mouse skeletal muscle, but activates all three γ subunit complexes in cultured human cells by inhibiting the respiratory chain

Articles

Pancreatic β-cell identity, glucose sensing and the control of insulin secretion

Guy A. Rutter, Timothy J. Pullen, David J. Hodson, Aida Martinez-Sanchez

Biochem J (2015) 466 (2): 203–218.

https://doi.org/10.1042/BJ20141384

Published: 20 February 2015

...Guy A. Rutter; Timothy J. Pullen; David J. Hodson; Aida Martinez-Sanchez Insulin release from pancreatic β-cells is required to maintain normal glucose homoeostasis in man and many other animals. Defective insulin secretion underlies all forms of diabetes mellitus, a disease currently reaching...

View article titled, Pancreatic β-cell identity, glucose sensing and the control of insulin secretion

Articles

Hepatic serum- and glucocorticoid-regulated protein kinase 1 (SGK1) regulates insulin sensitivity in mice via extracellular-signal-regulated kinase 1/2 (ERK1/2)

Hao Liu, Junjie Yu, Tingting Xia, Yuzhong Xiao, Qian Zhang, Bin Liu, Yajie Guo, Jiali Deng, Yalan Deng, Shanghai Chen, Aniko Naray-Fejes-Toth, Geza Fejes-Toth, Feifan Guo

Biochem J (2014) 464 (2): 281–289.

https://doi.org/10.1042/BJ20141005

Published: 14 November 2014

... (serum- and glucocorticoid-regulated kinase 1) have been identified, a direct effect of SGK1 on insulin sensitivity has not been previously reported. In the present study, we generated liver-specific SGK1-knockout mice and found that these mice developed glucose intolerance and insulin resistance. We...

View article titled, Hepatic serum- and glucocorticoid-regulated protein kinase 1 (SGK1) regulates insulin sensitivity in mice via extracellular-signal-regulated kinase 1/2 (ERK1/2)

Includes: Supplementary data

Articles

A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration

Nordine Helassa, James P. Garnett, Matthew Farrant, Faaizah Khan, John C. Pickup, Klaus M. Hahn, Christopher J. MacNevin, Robert Tarran, Deborah L. Baines

Biochem J (2014) 464 (2): 213–220.

https://doi.org/10.1042/BJ20141041

Published: 14 November 2014

...Nordine Helassa; James P. Garnett; Matthew Farrant; Faaizah Khan; John C. Pickup; Klaus M. Hahn; Christopher J. MacNevin; Robert Tarran; Deborah L. Baines Both lung disease and elevation of blood glucose are associated with increased glucose concentration (from 0.4 to ~4.0 mM) in the airway surface...

View article titled, A novel fluorescent sensor protein for detecting changes in airway surface liquid glucose concentration

Includes: Supplementary data

Articles

Non-preferential fuelling of the Na + /K + -ATPase pump

Ignacio Fernández-Moncada, L. Felipe Barros

Biochem J (2014) 460 (3): 353–361.

https://doi.org/10.1042/BJ20140003

Published: 29 May 2014

... advantage of the high temporal resolution of recently available FRET nanosensors for glucose, lactate and ATP. The activity of the Na + pump was assessed in parallel with the Na + -sensitive dye SBFI AM (Na + -binding benzofuran isophthalate acetoxymethyl ester). OXPHOS (oxidative phosphorylation...

View article titled, Non-preferential fuelling of the Na + /K + -ATPase pump

Includes: Supplementary data

Articles

Differential regulation of glucose transport activity in yeast by specific cAMP signatures

Clara Bermejo, Farzad Haerizadeh, Mayuri S. C. Sadoine, Diane Chermak, Wolf B. Frommer

Biochem J (2013) 452 (3): 489–497.

https://doi.org/10.1042/BJ20121736

Published: 31 May 2013

... to glucose by activating the Hxt5p (hexose transporter 5) glucose transporter, which provides an advantage during early phases after glucose resupply. cAMP and glucose FRET (fluorescence resonance energy transfer) sensors were used to identify three signalling pathways that co-operate in the anticipatory...

View article titled, Differential regulation of glucose transport activity in yeast by specific cAMP signatures

Includes: Supplementary data

Articles

Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models

Emmanuelle Cognard, Coralie G. Dargaville, Deborah L. Hay, Peter R. Shepherd

Biochem J (2013) 449 (3): 803–811.

https://doi.org/10.1042/BJ20121454

Published: 09 January 2013

...Emmanuelle Cognard; Coralie G. Dargaville; Deborah L. Hay; Peter R. Shepherd Pancreatic β-cells are highly responsive to changes in glucose, but the mechanisms involved are only partially understood. There is increasing evidence that the β-catenin signalling pathway plays an important role...

View article titled, Identification of a pathway by which glucose regulates β-catenin signalling via the cAMP/protein kinase A pathway in β-cell models

Includes: Supplementary data

Articles

Glucose induces autophagy under starvation conditions by a p38 MAPK-dependent pathway

Jose Félix Moruno-Manchón, Eva Pérez-Jiménez, Erwin Knecht

Biochem J (2013) 449 (2): 497–506.

https://doi.org/10.1042/BJ20121122

Published: 14 December 2012

... or organelles. In response to nutrient deprivation, e.g. depletion of amino acids or serum, autophagy is induced and most of these signals converge on the kinase mTOR (mammalian target of rapamycin). It is commonly accepted that glucose inhibits autophagy, since its deprivation from cells cultured in full...

View article titled, Glucose induces autophagy under starvation conditions by a p38 MAPK-dependent pathway

Includes: Supplementary data

Articles

Fasting induces ketoacidosis and hypothermia in PDHK2/PDHK4-double-knockout mice

Nam Ho Jeoung, Yasmeen Rahimi, Pengfei Wu, W. N. Paul Lee, Robert A. Harris

Biochem J (2012) 443 (3): 829–839.

https://doi.org/10.1042/BJ20112197

Published: 16 April 2012

... activity and lower blood glucose levels in the fed, but not the fasted, state. PDHK4 deficiency caused similar effects, but only after fasting. Double deficiency intensified these effects in both the fed and fasted states. PDHK2 deficiency had no effect on glucose tolerance, PDHK4 deficiency produced only...

View article titled, Fasting induces ketoacidosis and hypothermia in PDHK2/PDHK4-double-knockout mice

Articles

Dynamic measurements of mitochondrial hydrogen peroxide concentration and glutathione redox state in rat pancreatic β-cells using ratiometric fluorescent proteins: confounding effects of pH with HyPer but not roGFP1

Leticia P. Roma, Jessica Duprez, Hilton K. Takahashi, Patrick Gilon, Andreas Wiederkehr, Jean-Christophe Jonas

Biochem J (2012) 441 (3): 971–978.

https://doi.org/10.1042/BJ20111770

Published: 16 January 2012

...Leticia P. Roma; Jessica Duprez; Hilton K. Takahashi; Patrick Gilon; Andreas Wiederkehr; Jean-Christophe Jonas Using the ROS (reactive oxygen species)-sensitive fluorescent dyes dichlorodihydrofluorescein and dihydroethidine, previous studies yielded opposite results about the glucose regulation...

View article titled, Dynamic measurements of mitochondrial hydrogen peroxide concentration and glutathione redox state in rat pancreatic β-cells using ratiometric fluorescent proteins: confounding effects of pH with HyPer but not roGFP1

Articles

Mutational analysis of allosteric activation and inhibition of glucokinase

Bogumil Zelent, Stella Odili, Carol Buettger, Dorothy K. Zelent, Pan Chen, Deborah Fenner, Joseph Bass, Charles Stanley, Monique Laberge, Jane M. Vanderkooi, Ramakanth Sarabu, Joseph Grimsby, Franz M. Matschinsky

Biochem J (2011) 440 (2): 203–215.

https://doi.org/10.1042/BJ20110440

Published: 14 November 2011

...Bogumil Zelent; Stella Odili; Carol Buettger; Dorothy K. Zelent; Pan Chen; Deborah Fenner; Joseph Bass; Charles Stanley; Monique Laberge; Jane M. Vanderkooi; Ramakanth Sarabu; Joseph Grimsby; Franz M. Matschinsky GK (glucokinase) is activated by glucose binding to its substrate site, is inhibited...

View article titled, Mutational analysis of allosteric activation and inhibition of glucokinase

Includes: Supplementary data

Articles

ER stress modulates cellular metabolism

Xiaoli Wang, Colins O. Eno, Brian J. Altman, Yanglong Zhu, Guoping Zhao, Kristen E. Olberding, Jeffrey C. Rathmell, Chi Li

Biochem J (2011) 435 (1): 285–296.

https://doi.org/10.1042/BJ20101864

Published: 15 March 2011

...-resolved metabolomics) to determine the metabolic effects of tunicamycin. Glucose was found to be the major carbon source for energy production and anabolic metabolism. Following tunicamycin exposure, glucose uptake and lactate production are greatly reduced. Decreased 13 C labelling in several cellular...

View article titled, ER stress modulates cellular metabolism

Includes: Supplementary data

Articles

Glucose promotion of GABA metabolism contributes to the stimulation of insulin secretion in β-cells

Javier Pizarro-Delgado, Matthias Braun, Inés Hernández-Fisac, Rafael Martín-Del-Río, Jorge Tamarit-Rodriguez

Biochem J (2010) 431 (3): 381–390.

https://doi.org/10.1042/BJ20100714

Published: 11 October 2010

... semialdehyde is increased by 2-oxoglutarate, generated in α-keto acid transamination to its corresponding α-amino acid. The present work was aimed at investigating whether glucose also promotes islet GABA metabolism and whether the latter contributes to the stimulation of insulin secretion. Glucose (20 mM...

View article titled, Glucose promotion of GABA metabolism contributes to the stimulation of insulin secretion in β-cells

Includes: Supplementary data

Articles

p38 MAPK /p53 signalling axis mediates neuronal apoptosis in response to tetrahydrobiopterin-induced oxidative stress and glucose uptake inhibition: implication for neurodegeneration

Simone Cardaci, Giuseppe Filomeni, Giuseppe Rotilio, Maria R. Ciriolo

Biochem J (2010) 430 (3): 439–451.

https://doi.org/10.1042/BJ20100503

Published: 27 August 2010

... (buthionine sulfoximine). We also demonstrated that BH4 impairs glucose uptake and utilization, which was prevented by catalase administration. This effect contributes to the neuronal demise, exacerbating BH4-induced nuclear damage and the activation of the pro-apoptotic p38 MAPK /p53 axis. Inhibition...

View article titled, p38 MAPK /p53 signalling axis mediates neuronal apoptosis in response to tetrahydrobiopterin-induced oxidative stress and glucose uptake inhibition: implication for neurodegeneration

Includes: Supplementary data

Articles

Silencing of the mitochondrial NADH shuttle component aspartate–glutamate carrier AGC1/Aralar1 in INS-1E cells and rat islets

Marina Casimir, Blanca Rubi, Francesca Frigerio, Gaelle Chaffard, Pierre Maechler

Biochem J (2009) 424 (3): 459–466.

https://doi.org/10.1042/BJ20090729

Published: 10 December 2009

... for the control of glucose-stimulated insulin secretion. AGC1 or Aralar1 (aspartate–glutamate carrier 1) is a key component of the malate–aspartate shuttle. Overexpression of AGC1 increases the capacity of the malate–aspartate shuttle, resulting in enhanced metabolism–secretion coupling, both in INS-1E cells...

View article titled, Silencing of the mitochondrial NADH shuttle component aspartate–glutamate carrier AGC1/Aralar1 in INS-1E cells and rat islets

Articles

Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion

Lynley D. Pound, Suparna A. Sarkar, Richard K. P. Benninger, Yingda Wang, Adisak Suwanichkul, Melanie K. Shadoan, Richard L. Printz, James K. Oeser, Catherine E. Lee, David W. Piston, Owen P. McGuinness, John C. Hutton, David R. Powell, Richard M. O'Brien

Biochem J (2009) 421 (3): 371–376.

https://doi.org/10.1042/BJ20090530

Published: 15 July 2009

... glucose levels were unchanged in 16-week-old, 6 h fasted animals of either gender; however, plasma insulin concentrations were reduced in both female (∼31%) and male (∼47%) ZnT-8 −/− mice. Intraperitoneal glucose tolerance tests demonstrated no impairment in glucose clearance in male ZnT-8 −/− mice...

View article titled, Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion

Includes: Supplementary data

Articles

Glucose regulation of insulin gene expression in pancreatic β-cells

Sreenath S. Andrali, Megan L. Sampley, Nathan L. Vanderford, Sabire Özcan

Biochem J (2008) 415 (1): 1–10.

https://doi.org/10.1042/BJ20081029

Published: 12 September 2008

... to changes in blood glucose levels. The synthesis of insulin is regulated by blood glucose levels at the transcriptional and post-transcriptional levels. Although many transcription factors have been implicated in the regulation of insulin gene transcription, three β-cell-specific transcriptional regulators...

View article titled, Glucose regulation of insulin gene expression in pancreatic β-cells

Includes: Multimedia, Supplementary data

Articles

Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae

Robert N. Campbell, Michael K. Leverentz, Louise A. Ryan, Richard J. Reece

Biochem J (2008) 414 (2): 177–187.

https://doi.org/10.1042/BJ20080923

Published: 12 August 2008

... © The Authors Journal compilation © 2008 Biochemical Society 2008 galactose glucose haem meiosis oxygen The Zn(II) 2 Cys 6 family of proteins are defined by a conserved motif. This motif is composed of six cysteine residues in the form of: -Cys-X 2 -Cys-X 6 -Cys-X 5-12 -Cys-X 2...

View article titled, Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae

Articles

Identification and function of phosphorylation in the glucose-regulated transcription factor ChREBP

Nikolas G. Tsatsos, Michael N. Davies, Brennon L. O'callaghan, Howard C. Towle

Biochem J (2008) 411 (2): 261–270.

https://doi.org/10.1042/BJ20071156

Published: 27 March 2008

...Nikolas G. Tsatsos; Michael N. Davies; Brennon L. O'callaghan; Howard C. Towle In the liver, induction of genes encoding enzymes involved in de novo lipogenesis occurs in response to increased glucose metabolism. ChREBP (carbohydrate-response-element-binding protein) is a basic helix–loop–helix...

View article titled, Identification and function of phosphorylation in the glucose-regulated transcription factor ChREBP

Includes: Supplementary data

Articles

A decrease in cellular energy status stimulates PERK-dependent eIF2α phosphorylation and regulates protein synthesis in pancreatic β-cells

Edith Gomez, Mike L. Powell, Alan Bevington, Terence P. Herbert

Biochem J (2008) 410 (3): 485–493.

https://doi.org/10.1042/BJ20071367

Published: 27 February 2008

...Edith Gomez; Mike L. Powell; Alan Bevington; Terence P. Herbert In the present study, we demonstrate that, in pancreatic β-cells, eIF2α (eukaryotic initiation factor 2α) phosphorylation in response to a decrease in glucose concentration is primarily mediated by the activation of PERK [PKR (protein...

View article titled, A decrease in cellular energy status stimulates PERK-dependent eIF2α phosphorylation and regulates protein synthesis in pancreatic β-cells

Articles

Effect of glycation on α-crystallin structure and chaperone-like function

P. Anil Kumar, M. Satish Kumar, G. Bhanuprakash Reddy

Biochem J (2007) 408 (2): 251–258.

https://doi.org/10.1042/BJ20070989

Published: 14 November 2007

... glycation end-products) in various tissues, including the lens. Nevertheless, glycation of α-crystallin with various sugars has resulted in divergent results. In the present in vitro study, we have investigated the effect of glucose, fructose, G6P (glucose 6-phosphate) and MGO (methylglyoxal), which...

View article titled, Effect of glycation on α-crystallin structure and chaperone-like function

Articles

Oxo-4-methylpentanoic acid directs the metabolism of GABA into the Krebs cycle in rat pancreatic islets

Inés Hernández-Fisac, Sergio Fernández-Pascual, Henrik Ortsäter, Javier Pizarro-Delgado, Rafael Martín Del Río, Peter Bergsten, Jorge Tamarit-Rodriguez

Biochem J (2006) 400 (1): 81–89.

https://doi.org/10.1042/BJ20060173

Published: 27 October 2006

.... The L -[U- 14 C]-Glutamine (at 0.5 and 10.0 mM) conversion to 14 CO 2 was enhanced by 10 mM OMP within 30% and 70% respectively. Gabaculine (250 μM), a GABA transaminase inhibitor, suppressed OMP-induced oxygen consumption but not L -leucine- or glucose-stimulated respiration. It also suppressed the OMP...

View article titled, Oxo-4-methylpentanoic acid directs the metabolism of GABA into the Krebs cycle in rat pancreatic islets

Articles

The fructosamine 3-kinase knockout mouse: a tool for testing the glycation hypothesis of intracellular protein damage in diabetes and aging

Vincent M. Monnier

Biochem J (2006) 399 (2): e11.

https://doi.org/10.1042/BJ20061232

Published: 27 September 2006

... phosphorylation of glucose-derived Amadori products suggests that intracellular glycation could be deleterious under certain circumstances. In order to approach the question of the biological relevance of intracellular glycation, in this issue of the Biochemical Journal , Veiga-da-Cunha and colleagues generated...

View article titled, The fructosamine 3-kinase knockout mouse: a tool for testing the glycation hypothesis of intracellular protein damage in diabetes and aging

Articles

Analysis of hepatic gene transcription in mice expressing insulin-insensitive GSK3

Christopher Lipina, Xu Huang, David Finlay, Edward J McManus, Dario R. Alessi, Calum Sutherland

Biochem J (2005) 392 (3): 633–639.

https://doi.org/10.1042/BJ20051046

Published: 06 December 2005

... of blood glucose levels, while high GSK3 activity has been reported in Type II diabetes. Insulin inhibits GSK3 by promoting phosphorylation of a serine residue (Ser-21 in GSK3α, Ser-9 in GSK3β), thereby relieving GSK3 inhibition of glycogen synthesis in muscle. GSK3 inhibition in liver reduces expression...

View article titled, Analysis of hepatic gene transcription in mice expressing insulin-insensitive GSK3

Articles

Deficiency of PDK1 in liver results in glucose intolerance, impairment of insulin-regulated gene expression and liver failure

Alfonso MORA, Christopher LIPINA, François TRONCHE, Calum SUTHERLAND, Dario R. ALESSI

Biochem J (2005) 385 (3): 639–648.

https://doi.org/10.1042/BJ20041782

Published: 24 January 2005

...Alfonso MORA; Christopher LIPINA; François TRONCHE; Calum SUTHERLAND; Dario R. ALESSI The liver plays an important role in insulin-regulated glucose homoeostasis. To study the function of the PDK1 (3-phosphoinositide-dependent protein kinase-1) signalling pathway in mediating insulin's actions...

View article titled, Deficiency of PDK1 in liver results in glucose intolerance, impairment of insulin-regulated gene expression and liver failure

Articles

5-Aminoimidazole-4-carboxamide riboside (AICAR) enhances GLUT2-dependent jejunal glucose transport: a possible role for AMPK

John WALKER, Humberto B. JIJON, Hugo DIAZ, Payam SALEHI, Thomas CHURCHILL, Karen L. MADSEN

Biochem J (2005) 385 (2): 485–491.

https://doi.org/10.1042/BJ20040694

Published: 07 January 2005

... ATP-dependent processes. The purpose of the present study was to determine the role of AMPK in regulating intestinal glucose transport. [ 3 H]3- O -methyl glucose fluxes were measured in murine jejunum in the presence and absence of the AMPK activators AICAR (5-aminoimidazole-4-carboxamide riboside...

View article titled, 5-Aminoimidazole-4-carboxamide riboside (AICAR) enhances GLUT2-dependent jejunal glucose transport: a possible role for AMPK

Articles

Analysis of Plasmodium vivax hexose transporters and effects of a parasitocidal inhibitor

Thierry JOËT, Kesinee CHOTIVANICH, Kamolrat SILAMUT, Asha P. PATEL, Christophe MORIN, Sanjeev KRISHNA

Biochem J (2004) 381 (3): 905–909.

https://doi.org/10.1042/BJ20040433

Published: 27 July 2004

... resistance to current treatments. To study new potential targets, we have functionally characterized two natural variants of the hexose transporter of P. vivax (PvHT) after heterologous expression in Xenopus oocytes. We show that PvHT transports both glucose and fructose. Differences in the affinity...

View article titled, Analysis of Plasmodium vivax hexose transporters and effects of a parasitocidal inhibitor

Articles

Distinct roles for insulin and insulin-like growth factor-1 receptors in pancreatic beta-cell glucose sensing revealed by RNA silencing

Gabriela da SILVA XAVIER, Qingwen QIAN, Peter J. CULLEN, Guy A. RUTTER

Biochem J (2004) 377 (1): 149–158.

https://doi.org/10.1042/bj20031260

Published: 01 January 2004

...Gabriela da SILVA XAVIER; Qingwen QIAN; Peter J. CULLEN; Guy A. RUTTER The importance of the insulin receptor (IR) and the insulin-like growth factor-1 receptor (IGF-1R) for glucose-regulated insulin secretion and gene expression in pancreatic islet β-cells is at present unresolved. Here, we have...

View article titled, Distinct roles for insulin and insulin-like growth factor-1 receptors in pancreatic beta-cell glucose sensing revealed by RNA silencing

Articles

Regulation of type II transforming-growth-factor-β receptors by protein kinase C ι

Lea-Yea CHUANG, Jinn-Yuh GUH, Shu-Fen LIU, Min-Yuan HUNG, Tung-Nan LIAO, Tai-An CHIANG, Jau-Shyang HUANG, Yu-Lun HUANG, Chi-Fong LIN, Yu-Lin YANG

Biochem J (2003) 375 (2): 385–393.

https://doi.org/10.1042/bj20030522

Published: 15 October 2003

... TGF-β receptor (TβRII) induced by high glucose might contribute to distal tubular hypertrophy [Yang, Guh, Yang, Lai, Tsai, Hung, Chang and Chuang (1998) J. Am. Soc. Nephrol. 9 , 182–193]. We have elucidated the mechanism by using cultured Madin–Darby canine kidney cells. Enhancer assay...

View article titled, Regulation of type II transforming-growth-factor-β receptors by protein kinase C ι

Articles

Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2

Florence GOUYON, Cercina ONESTO, Veronique DALET, Gilles PAGES, Armelle LETURQUE, Edith BROT-LAROCHE

Biochem J (2003) 375 (1): 167–174.

https://doi.org/10.1042/bj20030661

Published: 01 October 2003

...Florence GOUYON; Cercina ONESTO; Veronique DALET; Gilles PAGES; Armelle LETURQUE; Edith BROT-LAROCHE In intestinal cells, levels of the fructose transporter GLUT5 are increased by glucose and to a greater extent by fructose. We investigated the mechanism by which fructose increases GLUT5 expression...

View article titled, Fructose modulates GLUT5 mRNA stability in differentiated Caco-2 cells: role of cAMP-signalling pathway and PABP (polyadenylated-binding protein)-interacting protein (Paip) 2

Articles

γIII-Crystallin is the primary target of glycation in the bovine lens incubated under physiological conditions

Hong YAN, Antony C. WILLIS, John J. HARDING

Biochem J (2003) 374 (3): 677–685.

https://doi.org/10.1042/bj20030542

Published: 15 September 2003

...Hong YAN; Antony C. WILLIS; John J. HARDING Several mechanisms have been proposed for the way in which glucose and its metabolites cause cataract, retinopathy and other complications of diabetes, the most convincing being glycation. Glycation, the reaction of sugars with free amino groups...

View article titled, γIII-Crystallin is the primary target of glycation in the bovine lens incubated under physiological conditions

Articles

Role for AMP-activated protein kinase in glucose-stimulated insulin secretion and preproinsulin gene expression

Gabriela da SILVA XAVIER, Isabelle LECLERC, Aniko VARADI, Takashi TSUBOI, S. Kelly MOULE, Guy A. RUTTER

Biochem J (2003) 371 (3): 761–774.

https://doi.org/10.1042/bj20021812

Published: 01 May 2003

... achieved pharmacologically with 5-amino-4-imidazolecarboxamide riboside (AICAR), or by adenoviral overexpression of a truncated, constitutively active form of the enzyme (AMPKα1.T 172 D), blocked glucose-stimulated insulin secretion. In MIN6 cells, activation of AMPK suppressed glucose metabolism...

View article titled, Role for AMP-activated protein kinase in glucose-stimulated insulin secretion and preproinsulin gene expression

Articles

Proteomic response to physiological fermentation stresses in a wild-type wine strain of Saccharomyces cerevisiae

Lorenza TRABALZINI, Alessandro PAFFETTI, Andrea SCALONI, Fabio TALAMO, Elisa FERRO, Grazietta CORATZA, Lucia BOVALINI, Paola LUSINI, Paola MARTELLI, Annalisa SANTUCCI

Biochem J (2003) 370 (1): 35–46.

https://doi.org/10.1042/bj20020140

Published: 15 February 2003

... grape must, to mild and progressive physiological stresses due to fermentation. We observed by two-dimensional electrophoresis how the yeast proteome changes during glucose exhaustion, before the cell enters its complete stationary phase. On the basis of their identification, the proteins representing...

View article titled, Proteomic response to physiological fermentation stresses in a wild-type wine strain of Saccharomyces cerevisiae

Articles

Comparative characterization of hexose transporters of Plasmodium knowlesi, Plasmodium yoelii and Toxoplasma gondii highlights functional differences within the apicomplexan family

Thierry JOËT, Lennart HOLTERMAN, Timothy T. STEDMAN, Clemens H.M. KOCKEN, Annemarie van der WEL, Alan W. THOMAS, Sanjeev KRISHNA

Biochem J (2002) 368 (3): 923–929.

https://doi.org/10.1042/bj20021189

Published: 15 December 2002

..., is a promising new drug target because asexual-stage malarial parasites depend wholly on glucose for energy. We have performed a comparative functional characterization of PfHT1 and hexose transporters of the simian malarial parasite P. knowlesi (PkHT1), the rodent parasite P. yoelii (PyHT1) and the human...

View article titled, Comparative characterization of hexose transporters of Plasmodium knowlesi, Plasmodium yoelii and Toxoplasma gondii highlights functional differences within the apicomplexan family

Articles

Protein kinase A translocation and insulin secretion in pancreatic β-cells: studies with adenylate cyclase toxin from Bordetella pertussis

Zhiyong GAO, Robert A. YOUNG, Matteo M. TRUCCO, Scott R. GREENE, Erik L. HEWLETT, Franz M. MATSCHINSKY, Bryan A. WOLF

Biochem J (2002) 368 (2): 397–404.

https://doi.org/10.1042/bj20020999

Published: 01 December 2002

... cyclase activity, and forskolin, an activator of adenylate cyclase, both dose-dependently increased insulin secretion in the presence, but not the absence, of glucose in insulin-secreting βTC3 cells. The stimulation of cAMP release by either agent was dose-dependent but glucose-independent. Omission...

View article titled, Protein kinase A translocation and insulin secretion in pancreatic β-cells: studies with adenylate cyclase toxin from Bordetella pertussis

Articles

Chromatographic assay of glycation adducts in human serum albumin glycated in vitro by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and intrinsic fluorescence

Naila AHMED, Paul J. THORNALLEY

Biochem J (2002) 364 (1): 15–24.

https://doi.org/10.1042/bj3640015

Published: 08 May 2002

...Naila AHMED; Paul J. THORNALLEY Glycation of proteins leads to the formation of advanced glycation endproducts (AGEs) of diverse molecular structure and biological function. Serum albumin derivatives modified to minimal and high extents by methylglyoxal and glucose in vitro have been used in many...

View article titled, Chromatographic assay of glycation adducts in human serum albumin glycated in vitro by derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and intrinsic fluorescence

Articles

Contribution by different fuels and metabolic pathways to the total ATP turnover of proliferating MCF-7 breast cancer cells

Michael GUPPY, Peter LEEDMAN, XinLin ZU, Victoria RUSSELL

Biochem J (2002) 364 (1): 309–315.

https://doi.org/10.1042/bj3640309

Published: 08 May 2002

...Michael GUPPY; Peter LEEDMAN; XinLin ZU; Victoria RUSSELL For the past 70 years the dominant perception of cancer metabolism has been that it is fuelled mainly by glucose (via aerobic glycolysis) and glutamine. Consequently, investigations into the diagnosis, treatment and the basic metabolism...

View article titled, Contribution by different fuels and metabolic pathways to the total ATP turnover of proliferating MCF-7 breast cancer cells

Articles

Assay of advanced glycation endproducts (AGEs): surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to N∊-carboxymethyl-lysine- and N∊-(1-carboxyethyl)lysine-modified albumin

Naila AHMED, Ognian K. ARGIROV, Harjit S. MINHAS, Carlos A.A. CORDEIRO, Paul J. THORNALLEY

Biochem J (2002) 364 (1): 1–14.

https://doi.org/10.1042/bj3640001

Published: 08 May 2002

...-lysine and N ∊ -(1-carboxyethyl)-lysine. 3-deoxyglucosone fructosamine glucose glyoxal methylglyoxal Abbreviations used: AGE, advanced glycation endproduct; AQC, 6-aminoquinolyl- N -hydroxysuccinimidyl-carbamate; CEL, N ∊ -(1-carboxyethyl)lysine; CML, N ∊ -carboxymethyl-lysine...

View article titled, Assay of advanced glycation endproducts (AGEs): surveying AGEs by chromatographic assay with derivatization by 6-aminoquinolyl-N-hydroxysuccinimidyl-carbamate and application to N∊-carboxymethyl-lysine- and N∊-(1-carboxyethyl)lysine-modified albumin

Articles

Dietary and developmental regulation of intestinal sugar transport

Ronaldo P. FERRARIS

Biochem J (2001) 360 (2): 265–276.

https://doi.org/10.1042/bj3600265

Published: 26 November 2001

...Ronaldo P. FERRARIS The Na + -dependent glucose transporter SGLT1 and the facilitated fructose transporter GLUT5 absorb sugars from the intestinal lumen across the brush-border membrane into the cells. The activity of these transport systems is known to be regulated primarily by diet...

View article titled, Dietary and developmental regulation of intestinal sugar transport

Articles

Spot 14 protein interacts and co-operates with chicken ovalbumin upstream promoter-transcription factor 1 in the transcription of the L-type pyruvate kinase gene through a specificity protein 1 (Sp1) binding site

Emmanuel COMPE, Georges de SOUSA, Kamel FRANCÇOIS, Régis ROCHE, Roger RAHMANI, Janine TORRESANI, Michel RAYMONDJEAN, Richard PLANELLS

Biochem J (2001) 358 (1): 175–183.

https://doi.org/10.1042/bj3580175

Published: 08 August 2001

...-transcription factor 1 (COUP-TF1) and induces the switch of this factor from a repressor to an activator. However, the enhancing activity of S14 and COUP-TF1 depends on the presence of a proximal GC-rich box (the L0 element) that specifically binds nuclear proteins from the livers of rats fed a glucose-rich...

View article titled, Spot 14 protein interacts and co-operates with chicken ovalbumin upstream promoter-transcription factor 1 in the transcription of the L-type pyruvate kinase gene through a specificity protein 1 (Sp1) binding site

Articles

Angiotensin II-responsive element is the insulin-responsive element in the adipocyte fatty acid synthase gene: role of adipocyte determination and differentiation factor 1/sterol-regulatory-element-binding protein 1c

Suyeon KIM, Isabelle DUGAIL, Melissa STANDRIDGE, Kate CLAYCOMBE, Joseph CHUN, Naïma MOUSTAÏD-MOUSSA

Biochem J (2001) 357 (3): 899–904.

https://doi.org/10.1042/bj3570899

Published: 25 July 2001

...-regulated by Ang II. With the use of transfections as well as glucose transport assays, we further demonstrated that Ang II stimulation of the FAS gene was dependent on glucose. In conclusion, this is the first report that Ang II regulates adipocyte FAS gene transcription via insulin response sequences...

View article titled, Angiotensin II-responsive element is the insulin-responsive element in the adipocyte fatty acid synthase gene: role of adipocyte determination and differentiation factor 1/sterol-regulatory-element-binding protein 1c

Articles

Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK

Diana L. LEFEBVRE, Yahong BAI, Nazanin SHAHMOLKY, Monika SHARMA, Raymond POON, Daniel J. DRUCKER, Cheryl F. ROSEN

Biochem J (2001) 355 (2): 297–305.

https://doi.org/10.1042/bj3550297

Published: 06 April 2001

...-amino-4-imidazolecarboxamide riboside (AICAriboside) in rat keratinocyte cells, implying that SNARK might be activated by an AMPK kinase-dependent pathway. Furthermore, glucose deprivation increased SNARK activity 3-fold in BHK fibroblasts. These findings identify SNARK as a glucose- and AICAriboside...

View article titled, Identification and characterization of a novel sucrose-non-fermenting protein kinase/AMP-activated protein kinase-related protein kinase, SNARK

Articles

Shared control of hepatic glycogen synthesis by glycogen synthase and glucokinase

Roger R. GOMIS, Juan C. FERRER, Joan J. GUINOVART

Biochem J (2000) 351 (3): 811–816.

https://doi.org/10.1042/bj3510811

Published: 24 October 2000

...Roger R. GOMIS; Juan C. FERRER; Joan J. GUINOVART We have used recombinant adenoviruses (AdCMV-RLGS and AdCMV-GK) to overexpress the liver isoforms of glycogen synthase (GS) and glucokinase (GK) in primary cultured rat hepatocytes. Glucose activated overexpressed GS in a dose-dependent manner...

View article titled, Shared control of hepatic glycogen synthesis by glycogen synthase and glucokinase

Articles

Role of plasma membrane transporters in muscle metabolism

Antonio ZORZANO, César FANDOS, Manuel PALACÍN

Biochem J (2000) 349 (3): 667–688.

https://doi.org/10.1042/bj3490667

Published: 25 July 2000

...Antonio ZORZANO; César FANDOS; Manuel PALACÍN Muscle plays a major role in metabolism. Thus it is a major glucose-utilizing tissue in the absorptive state, and changes in muscle insulin-stimulated glucose uptake alter whole-body glucose disposal. In some conditions, muscle preferentially uses lipid...

View article titled, Role of plasma membrane transporters in muscle metabolism

Articles

Identification of Nω-carboxymethylarginine as a novel acid-labile advanced glycation end product in collagen

Katsumasa IIJIMA, Michio MURATA, Hidenari TAKAHARA, Shinkichi IRIE, Daisaburou FUJIMOTO

Biochem J (2000) 347 (1): 23–27.

https://doi.org/10.1042/bj3470023

Published: 27 March 2000

...-assisted laster-desorption ionization-MS and NMR. CMA gradually increased in collagen during incubation with glucose and the yield reached about 8 mol/mol of collagen, which is 100 times higher than that of pentosidine. This result suggests that CMA is a major AGE in collagen. Biochem. J. (2000) 347, 23 27...

View article titled, Identification of Nω-carboxymethylarginine as a novel acid-labile advanced glycation end product in collagen

Articles

Nutrients differentially regulate multiple translation factors and their control by insulin

Linda E. CAMPBELL, Xuemin WANG, Christopher G. PROUD

Biochem J (1999) 344 (2): 433–441.

https://doi.org/10.1042/bj3440433

Published: 24 November 1999

... that their activation by insulin requires the presence, in the medium in which the cells are maintained, of both amino acids and glucose: insulin only induced activation of eIF2B and the dephosphorylation of eEF2 when cells were exposed to both types of nutrient. Other translational regulators, e.g. the 70 kDa...

View article titled, Nutrients differentially regulate multiple translation factors and their control by insulin

Articles

Protection against hydrogen peroxide cytotoxicity in Rat-1 fibroblasts provided by the oncoprotein Bcl-2: maintenance of calcium homoeostasis is secondary to the effect of Bcl-2 on cellular glutathione

Matthäus M. RIMPLER, Ursula RAUEN, Thorsten SCHMIDT, Tarik MÖRÖY, Herbert DE GROOT

Biochem J (1999) 340 (1): 291–297.

https://doi.org/10.1042/bj3400291

Published: 10 May 1999

... concentration. Stabilization of the glutathione pool by Bcl-2, N -acetylcysteine or glucose delayed the cytosolic calcium increase and subsequent cell death, whereas depletion of glutathione by D L -buthionine-( S , R )-sulphoximine, sensitized Bcl-2-transfected cells towards cytosolic calcium increase and cell...

View article titled, Protection against hydrogen peroxide cytotoxicity in Rat-1 fibroblasts provided by the oncoprotein Bcl-2: maintenance of calcium homoeostasis is secondary to the effect of Bcl-2 on cellular glutathione

Articles

Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein

Arthur L. KRUCKEBERG, Ling YE, Jan A. BERDEN, Karel van DAM

Biochem J (1999) 339 (2): 299–307.

https://doi.org/10.1042/bj3390299

Published: 08 April 1999

...Arthur L. KRUCKEBERG; Ling YE; Jan A. BERDEN; Karel van DAM The Hxt2 glucose transport protein of Saccharomyces cerevisiae was genetically fused at its C-terminus with the green fluorescent protein (GFP). The Hxt2-GFP fusion protein is a functional hexose transporter: it restored growth on glucose...

View article titled, Functional expression, quantification and cellular localization of the Hxt2 hexose transporter of Saccharomyces cerevisiae tagged with the green fluorescent protein

Email alerts

CONNECT

EXPLORE

This Feature Is Available To Subscribers Only