... on behalf of the Biochemical Society 2021 exercise glucose uptake insulin insulin sensitivity muscle metabolism physical activity Insulin is the primary anabolic hormone in the body that increases the deposition of energy substrates from food in the form of glycogen and lipid. In contrast...

..., but also inhibited insulin signalling, insulin-mediated/AMPK-independent glucose uptake, and AICA-riboside uptake. We performed an extended screen of SBI-0206965 against a panel of 140 human protein kinases in vitro , which showed SBI-0206965 inhibits several kinases, including members of AMPK-related...

... of the allosteric AMPK activators A-769662 and 991 on glucose uptake in adipocytes. For this purpose, primary rat or human adipocytes, and cultured 3T3-L1 adipocytes, were treated with either of the allosteric activators, or AICAR, and basal and insulin-stimulated glucose uptake was assessed. Additionally...

... by Portland Press Limited on behalf of the Biochemical Society 2016 glucose uptake hydrogen peroxide mitochondria oxidative stress Krebs-Ringer bicarbonate solution (KRB), used for all perifusion/imaging analysis, contained 98.5 mM NaCl, 4.9 mM KCl, 1.2 mM potassium phosphate, 1.2 mM...

...Nobuyuki Takenaka; Yukio Sumi; Keiko Matsuda; Junko Fujita; Tetsuya Hosooka; Tetsuya Noguchi; Atsu Aiba; Takaya Satoh Insulin-stimulated glucose uptake in skeletal muscle is mediated by the translocation of the glucose transporter GLUT4 from intracellular storage sites to the plasma membrane...

...James S. V. Lally; Gregory R. Steinberg Skeletal muscle is critical for whole-body glucose homoeostasis. Insulin and muscle contractions induced by exercise can increase glucose uptake through distinct intracellular signalling pathways involving PKB (protein kinase B)/Akt and AMPK (AMP-activated...

...-containing phosphatidylinositol 3-phosphate 5-kinase), the lipid kinase that phosphorylates PtdIns3 P to PtdIns(3,5) P 2 , has been implicated in insulin-stimulated glucose uptake. We investigated whether PIKfyve could also be involved in contraction/AMPK (AMP-activated protein kinase)-stimulated glucose...

... assessed the effects of the compounds on insulin-stimulated glucose uptake, glycogen and protein synthesis. MK-2206 dose-dependently inhibited insulin-stimulated PKB phosphorylation, PKBβ activity and phosphorylation of PKB downstream targets (including glycogen synthase kinase-3α/β, proline-rich Akt...

...-type littermates. BACE1 −/− mice are also protected from diet-induced obesity. BACE1-deficient skeletal muscle and liver exhibit improved insulin sensitivity. In a skeletal muscle cell line, BACE1 inhibition increased glucose uptake and enhanced insulin sensitivity. The loss of BACE1 is associated...

... on surface-exposed GLUT4–Myc in basal or insulin-stimulated cells, but markedly and selectively diminished insulin-stimulated 3- O -methyl glucose uptake and GLUT4–Myc photolabelling with ATB-BMPA {2- N -[4-(1-azitrifluoroethyl)benzoyl]-1,3-bis-( D -mannos-4-yloxy)-2-propylamine}, suggesting...

... Biochemical Society 2008 fluorescence correlation spectroscopy (FCS) glucose transporter-4 (GLUT4) glucose uptake insulin munc18c vesicle-associated membrane protein (VAMP) CHO (Chinese-hamster ovary; CHO-K1) cells were purchased from A.T.C.C. (A.T.C.C. number CCL-61; Manassas, VA, U.S.A...

... that insulin triggers interactions in vivo between PKCζ, 80K-H and munc18c. Overexpression of 80K-H constructs mimicked the action of insulin in stimulating both glucose uptake and translocation of Myc-tagged GLUT4 in Cho cells, with the level of effect proportional to the ability of the constructs...

...) [Almeida, Moncada and Bolaños (2004) Nat. Cell Biol. 6 , 45–51]. To determine whether glucose transport contributes specifically to this effect, we have now investigated the possible role of NO in modulating glucose uptake through GLUT3, a facilitative high-affinity glucose carrier that has been suggested...

... PKC isotypes are involved in the regulation of glucose transport is still poorly defined. PMA is a potent activator of conventional and novel PKCs and PMA increases the rate of glucose uptake in many different cell systems. In the present study, we show that PMA treatment increases glucose uptake...

... of the hormones. As a consequence, exposure of brown adipocytes to dexamethasone and insulin results in a dramatic increase of glucose uptake (12-fold). Dexamethasone induces the expression of CCAAT/enhancer-binding protein (C/EBP) α, insulin promotes myocyte enhancer factor-2 DNA-binding activity and both...

... of the ability of GS to bind UDP-glucose. 1 To whom correspondence should be addressed (e-mail [email protected] ). 21 8 2002 27 11 2002 2 12 2002 15 3 2003 The Biochemical Society, London ©2003 2003 glucose uptake glycogen synthesis insulin lactate phosphorylase...

... was reported to be activated by G i -coupled receptors via Gβγ subunits. We asked whether the stimulation of G i -coupled receptors would trigger GLUT4 translocation and glucose uptake by the activation of Gβγ-dependent p110γ PI-3K. We find that this translocation and glucose uptake can be induced...

...-fold the basal glucose uptake. Inhibition of phosphoinositide (PI) 3-kinase activity with chemical agents such as wortmannin or LY294002 partially blocked insulin-induced GLUT4 mRNA accumulation, insulin-induced GLUT4 protein content, GLUT4-CAT transactivation and glucose uptake. Furthermore, co...