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Keywords: glutathione
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Articles
Biochem J (2020) 477 (10): 1865–1878.
Published: 28 May 2020
..., including mixed disulfide formation by glutathionylation. The adjustment of the proper thiol redox state is a fundamental property of all cellular compartments. The glutathione redox potential of the cytosol, stroma, matrix and nucleoplasm usually ranges between −300 and −320 mV. Thiol reduction proceeds...
Articles
Biochem J (2019) 476 (13): 1857–1873.
Published: 02 July 2019
...Shambhu Yadav; Bindia Chawla; Mohammad Anwar Khursheed; Rajesh Ramachandran; Anand Kumar Bachhawat Calcium signaling is essential for embryonic development but the signals upstream of calcium are only partially understood. Here, we investigate the role of the intracellular glutathione redox...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2019) 476 (6): 965–974.
Published: 22 March 2019
... to a similar loss in germination speed in both genotypes that was lost faster under elevated O 2 . In both genotypes, an equal oxidative shift in the glutathione redox state and a minor loss of α-tocopherol progressively occurred before seed viability was lost. In contrast, ABA levels were not affected...
Includes: Supplementary data
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Biochem J (2019) 476 (4): 683–697.
Published: 19 February 2019
...Thomas D. Niehaus; Jenelle A. Patterson; Danny C. Alexander; Jakob S. Folz; Michal Pyc; Brian S. MacTavish; Steven D. Bruner; Robert T. Mullen; Oliver Fiehn; Andrew D. Hanson The tripeptide glutathione (GSH) is implicated in various crucial physiological processes including redox buffering...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (23): 3725–3743.
Published: 06 December 2018
... in the half-cell reduction potentials of low-molecular-weight (LMW) thiol-disulfide redox couples [i.e. glutathione disulfide (GSSG)/glutathione (GSH) and cystine/cysteine], alongside the activities of the reactive oxygen species (ROS)-processing enzyme superoxide dismutase, catalase, ascorbate peroxidase...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (7): 1175–1193.
Published: 15 March 2017
...Rachel Gergondey; Camille Garcia; Christophe H. Marchand; Stephane D. Lemaire; Jean-Michel Camadro; Françoise Auchère The potential biological consequences of oxidative stress and changes in glutathione levels include the oxidation of susceptible protein thiols and reversible covalent binding...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (6): 717–731.
Published: 10 March 2016
...), showing that DHAR active site is very selective for DHA recognition and providing further insights into the catalytic mechanism and the roles of the additional cysteines found in some DHARs. ascorbate recycling catalytic cysteine residue dehydroascorbate reductases glutathione nuclear magnetic...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (6): 693–701.
Published: 10 March 2016
...Louise V. Holyoake; Stuart Hunt; Guido Sanguinetti; Gregory M. Cook; Mark J. Howard; Michelle L. Rowe; Robert K. Poole; Mark Shepherd The glutathione/cysteine exporter CydDC maintains redox balance in Escherichia coli . A cydD mutant strain was used to probe the influence of CydDC upon reduced...
Includes: Supplementary data
Articles
Biochem J (2015) 472 (2): 217–223.
Published: 13 November 2015
... , 2667–2669]. Kinetic data indicate that Cox17 has reactivity similar to glutathione (GSH), the most abundant thiol-rich molecule in the cytoplasm. In the present study, we found that GSH significantly modulates the reaction of platinum complexes with Cox17. GSH enhances the reactivity of three anti...
Includes: Supplementary data
Articles
Biochem J (2014) 463 (1): 53–63.
Published: 08 September 2014
... profiling and affinity purification, together with the subsequent biochemical assays, we have elucidated the mechanism of action underlying NPD926-induced cell death: conjugation with glutathione mediated by GST, depletion of cellular glutathione and subsequent ROS generation. NPD926 preferentially induced...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (1): 39–52.
Published: 24 July 2014
.... Instead of promoting the conjugation of glutathione to acceptor molecules as do most GSTs, members of the Lambda class (GSTLs) catalyse deglutathionylation reactions via a catalytic cysteine residue. Three GSTL genes ( Pt-GSTL1 , Pt-GSTL2 and Pt-GSTL3 ) are present in Populus trichocarpa , but two...
Articles
Biochem J (2014) 460 (3): 425–435.
Published: 29 May 2014
... activity. The increased sensitivity towards H 2 O 2 -induced cytotoxicity in CSE-siRNA-transfected cells was associated with a decreased glutathione concentration (GSH) and glutathione ratio (GSH/GSSG). Incubation of cells with exogenous H 2 S increased the GSH concentration and GSH/GSSG ratio. Moreover...
Articles
Biochem J (2013) 450 (3): 547–557.
Published: 28 February 2013
...Stephanie Wickham; Nicholas Regan; Matthew B. West; Justin Thai; Paul F. Cook; Simon S. Terzyan; Pui Kai Li; Marie H. Hanigan GGT (γ-glutamyl transpeptidase) is an essential enzyme for maintaining cysteine homoeostasis, leukotriene synthesis, metabolism of glutathione conjugates and catabolism...
Articles
Biochem J (2013) 450 (3): 595–605.
Published: 28 February 2013
...-phosphate levels and production of reduced glutathione, as well as insulin secretion in INS-1 832/13 β-cells and rat islets without affecting ATP production. Metabolite profiling of rat islets confirmed the glucose-induced rise in ribose 5-phosphate, which was prevented by DHEA. These findings implicate...
Includes: Supplementary data
Articles
Biochem J (2013) 450 (1): 63–72.
Published: 24 January 2013
...William B. Musgrave; Hankuil Yi; Dustin Kline; Jeffrey C. Cameron; Jonathan Wignes; Sanghamitra Dey; Himadri B. Pakrasi; Joseph M. Jez Glutathione biosynthesis catalysed by GCL (glutamate-cysteine ligase) and GS (glutathione synthetase) is essential for maintaining redox homoeostasis and protection...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (3): 783–794.
Published: 09 January 2013
...Shailesh Kumar; Neha Kasturia; Amit Sharma; Manish Datt; Anand K. Bachhawat Glutathione is a thiol-containing tripeptide that plays important roles in redox-related processes. The first step in glutathione biosynthesis is catalysed by γ-GCS (γ-glutamylcysteine synthetase). The crystal structure...
Includes: Supplementary data
Articles
Biochem J (2012) 448 (2): 243–251.
Published: 07 November 2012
... measuring V-ATPase activity. © The Authors Journal compilation © 2012 Biochemical Society 2012 Arabidopsis thaliana glutathione nitrosoglutathione (GSNO) redox regulation vacuolar proton-translocating ATPase (V-ATPase) vacuole The catalytic V-ATPase subunit A is highly conserved...
Includes: Supplementary data
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Biochem J (2012) 446 (3): 333–348.
Published: 28 August 2012
...Elke Ströher; A. Harvey Millar Grxs (glutaredoxins) are small ubiquitous redox enzymes. They are generally involved in the reduction of oxidative modifications using glutathione. Grxs are not only able to reduce protein disulfides and the low-molecular-mass antioxidant dehydroascorbate, but also...
Includes: Supplementary data
Articles
Biochem J (2012) 445 (3): 423–430.
Published: 13 July 2012
... to oxidative stress caused by this compound. Further analyses revealed that only TR1-deficient cells manifested strongly enhanced production and secretion of glutathione, which was associated with increased sensitivity of the cells to selenite. The results suggest a new role for TR1 in cancer...
Includes: Supplementary data
Articles
Biochem J (2012) 442 (1): 191–197.
Published: 27 January 2012
..., and no efficient protein-mediated process has been identified. Recently, we observed that ferric cytochrome c can promote S -nitrosoglutathione formation from NO and glutathione by acting as an electron acceptor under anaerobic conditions. In the present study, we show that this mechanism is also robust under...
Articles
Biochem J (2012) 441 (1): 359–366.
Published: 14 December 2011
... correspondence should be addressed (email andersonet@ecu.edu ). 5 4 2011 19 8 2011 31 8 2011 31 8 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 aldehyde glutathione lipid peroxidation mitochondrion n −3 polyunsaturated fatty acid permeability...
Includes: Supplementary data
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Biochem J (2011) 440 (2): 175–183.
Published: 14 November 2011
... of the histone family; this is, to our knowledge, the first time that histone glutathionylation has been reported. Formation of the potential NO donor dinitrosyl–diglutathionyl–iron complex, bound to GSTP1-1 (glutathione transferase P1-1), was observed in both MCF7/Dx cells and drug-sensitive MCF7 cells...
Includes: Supplementary data
Articles
Biochem J (2011) 433 (2): 303–311.
Published: 22 December 2010
...-GLRX5, whereas the apoprotein is monomeric. MS analyses revealed glutathionylation of the cysteine residues in the absence of the [2Fe–2S] cluster, which would protect them from further oxidation and possibly facilitate cluster transfer/acceptance. Apo-GLRX5 reduced glutathione mixed disulfides...
Includes: Supplementary data
Articles
Biochem J (2010) 431 (2): 169–178.
Published: 28 September 2010
... Biochemical Society 2010 antioxidant cell cycle epigenome gene expression glutathione nucleus oxidative signalling poly(ADP-ribose) polymerase (PARP) redox state The paradigm concerning ROS (reactive oxygen species) that traditionally viewed these metabolites as potentially lethal...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2010) 430 (3): 439–451.
Published: 27 August 2010
...@bio.uniroma2.it ). 6 4 2010 17 6 2010 30 6 2010 30 6 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 apoptosis glucose glutathione mitogen-activated protein kinase (MAPK) p53 reactive oxygen species (ROS) Oxidative stress is considered...
Includes: Supplementary data
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Biochem J (2010) 429 (3): 593–602.
Published: 14 July 2010
...Anil Thakur; Anand K. Bachhawat Hgt1p, a high-affinity glutathione transporter from Saccharomyces cerevisiae belongs to the recently described family of OPTs (oligopeptide transporters), the majority of whose members still have unknown substrate specificity. To obtain insights into substrate...
Includes: Supplementary data
Articles
Biochem J (2009) 424 (2): 243–252.
Published: 11 November 2009
... of the genes up-regulated in Hfe −/− RPE cells was Slc7a11 (where Slc is solute carrier) which codes for the ‘transporter proper’ xCT in the heterodimeric cystine/glutamate exchanger (xCT/4F2hc). This transporter plays a critical role in cellular glutathione status and cell-cycle progression. We confirmed...
Includes: Supplementary data
Articles
Biochem J (2009) 421 (2): 293–299.
Published: 26 June 2009
..., such as the liver, brain, lung, adrenal grand and skeletal muscles. PEG–AOase slightly, but significantly, decreased glutathione (GSH) levels in the liver without affecting those in other tissues. Suppression of hepatic synthesis of GSH by administration of BSO [ L -buthionin-( S , R )-sulfoximine] enhanced the PEG...
Articles
Biochem J (2009) 417 (1): 269–277.
Published: 12 December 2008
... (email jr@sun.ac.za ). 28 5 2008 6 8 2008 12 8 2008 12 8 2008 © The Authors Journal compilation © 2009 Biochemical Society 2009 glutathione kinetic modelling NADPH redoxin reductase thiol Thioredoxins, glutaredoxins and peroxiredoxins (which we collectively...
Includes: Supplementary data
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Biochem J (2008) 414 (1): 93–102.
Published: 29 July 2008
... Biochemical Society 2008 anticancer drug toxicity cisplatin glutathione mitochondrial DNA (mtDNA) metabolism mitochondrial RNA (mtRNA) stability Wistar rats weighing 200–250 g were fed laboratory chow and water ad libitum and used for the experiments. In cisplatin in vivo studies, groups...
Articles
Biochem J (2008) 411 (2): 297–306.
Published: 27 March 2008
... cyclo-oxygenase (COX) 15-deoxy-Δ 12,14 -prostaglandin J 2 (15d-PGJ 2 ) glutathione haem (heme) oxygenase Kelch-like erythroid cell-derived protein with cap‘n’collar homology-associated protein 1 (Keap1) It has long been recognized that enzymes such as COX (cyclo-oxygenase) generate...
Articles
Biochem J (2008) 411 (1): 115–122.
Published: 13 March 2008
... that Tim10 can be oxidized by glutathione under cytosolic concentrations. However, it was unknown whether oxidative folding of other small Tims can occur under similar conditions and whether oxidative folding competes kinetically with mitochondrial import. In the present study, the effect of glutathione...
Articles
Biochem J (2007) 405 (3): 583–589.
Published: 13 July 2007
...Dikran Toroser; Rajindar S. Sohal The nature of the mechanisms underlying the age-related decline in glutathione (GSH) synthetic capacity is at present unclear. Steady-state kinetic parameters of mouse liver GCL (glutamate–cysteine ligase), the rate-limiting enzyme in GSH synthesis, and levels...
Articles
Biochem J (2006) 399 (1): 161–168.
Published: 13 September 2006
...D. Tim Harwood; Anthony J. Kettle; Christine C. Winterbourn GSH is rapidly oxidized by HOCl (hypochlorous acid), which is produced physiologically by the neutrophil enzyme myeloperoxidase. It is converted into, mainly, oxidized glutathione. Glutathione sulfonamide is an additional product...
Articles
Biochem J (2006) 396 (1): 61–69.
Published: 26 April 2006
...Arun Kumar; Lijo John; Md. Mahmood Alam; Ankit Gupta; Gayatri Sharma; Beena Pillai; Shantanu Sengupta Intracellular thiols like cysteine, homocysteine and glutathione play a critical role in the regulation of important cellular processes. Alteration of intracellular thiol concentration results...
Includes: Supplementary data
Articles
Biochem J (2006) 393 (1): 227–234.
Published: 12 December 2005
.... donovani GLOII showed a marked substrate specificity for trypanothione hemithioacetal over glutathione hemithioacetal. Antiserum against recombinant LdGLOII protein could detect a band of anticipated size ∼32 kDa in promastigote extracts. By overexpressing the GLOII gene in Leishmania donovani using...
Articles
Biochem J (2005) 392 (3): 693–701.
Published: 06 December 2005
... Society, London 2005 flash photolysis glutathione peroxyl radicals protein radicals pulse radiolysis rate constant Living organisms are constantly exposed to ROS (reactive oxygen species) more correctly designated here as PROS (partly reduced oxygen species) formed as by-products...
Articles
Biochem J (2005) 391 (2): 425–432.
Published: 10 October 2005
... is synthesized from glutathione and spermidine by a single enzyme, TryS (trypanothione synthetase), with glutathionylspermidine as an intermediate. To examine the physiological roles of trypanothione, tetracycline-inducible RNA interference was used to reduce expression of TRYS . Following induction, TryS...
Articles
Biochem J (2005) 389 (3): 785–795.
Published: 26 July 2005
... (glyceraldehyde-3-phosphate dehydrogenase) was striking, and other affected proteins included glutathione S-transferase P1-1, enolase, a regulatory subunit of protein kinase A, annexin VI, the mitotic checkpoint serine/threonine-protein kinase BUB1β, HSP90β (heat-shock protein 90β) and proteosome components...
Articles
Biochem J (2005) 388 (1): 93–101.
Published: 10 May 2005
...-sensitive mutants. Although many cellular components are potential targets, our studies indicate that mitochondrial glutathione is particularly vulnerable to hyperoxia damage. During hyperoxia stress, mitochondrial glutathione is more susceptible to oxidation than cytosolic glutathione. Furthermore, two...
Includes: Supplementary data
Articles
Biochem J (2005) 386 (2): 215–219.
Published: 22 February 2005
... production induced by EGF (epidermal growth factor) signalling. The glutathione redox state was measured by HPLC. Results showed that only cytosolic Trx1 undergoes significant oxidation. Thus EGF signalling involves subcellular compartmental oxidation of Trx1 in the absence of a generalized cellular...
Articles
Biochem J (2004) 383 (2): 335–341.
Published: 08 October 2004
... toxicity. 1 To whom correspondence should be addressed, at Liver Research Center (email ctliver@csf.units.it ). 13 4 2004 5 7 2004 8 7 2004 8 7 2004 The Biochemical Society, London 2004 ATP-binding-cassette protein (ABC) protein cytotoxicity glutathione...
Articles
Biochem J (2004) 383 (1): 139–147.
Published: 24 September 2004
... of 16 genes coding for thioredoxin- and glutathione-dependent redox system components. Quantifications were performed to examine the response to oxidants, to sudden temperature upshifts and in association with metabolic changes accompanying culture growth and to explore the contribution of mRNA decay...
Articles
Biochem J (2004) 382 (1): 131–136.
Published: 10 August 2004
... (glutathione, cysteine, Cys-Gly and methionine) and protein mixed disulphides were measured at different ages and ambient temperatures in Drosophila melanogaster . GSH/GSSG ratios decreased significantly with increasing age of the flies, due to an increase in GSSG content. Concentrations of Cys-Gly increased...
Articles
Biochem J (2004) 381 (3): e1.
Published: 27 July 2004
.... As an antioxidant, glutathione serves in maintaining the reduced state of cellular protein thiol groups. The paper by Cross and Templeton appearing in this issue of the Biochemical Journal describes a mechanism by which glutathionylation plays a key role in the regulation of the kinase activity of MEKK1 [MAP...
Articles
Biochem J (2004) 379 (3): 595–600.
Published: 01 May 2004
...-crystallin, with the γS-crystallin having glutathione bound at Cys-82 and at Cys-22, Cys-24 or Cys-26. We conclude that when glutathione becomes bound to γS-crystallin, it causes it to bind in turn to the β-crystallin polypeptides to form a dimer. 1 To whom correspondence should be addressed (e-mail...
Articles
Biochem J (2003) 376 (3): 725–732.
Published: 15 December 2003
... of the sphingolipid ceramide and reactive oxygen species are among these pathways. Crossregulation of these two pathways is possible owing to the demonstrated inhibition of neutral sphingomyelinase by glutathione, the predominant cellular antioxidant, and has been observed in some cytokine-dependent cell-death models...
Articles
Biochem J (2003) 376 (3): 807–812.
Published: 15 December 2003
...Gábor BÁNHEGYI; Miklós CSALA; Gábor NAGY; Vincenzo SORRENTINO; Rosella FULCERI; Angelo BENEDETTI In the present study, we have investigated the role of RyR1 (ryanodine receptor calcium channel type 1) in glutathione (GSH) transport through the sarcoplasmic reticulum (SR) membrane of skeletal...
Articles
Biochem J (2003) 374 (2): 513–519.
Published: 01 September 2003
...Daniel SHENTON; Chris M. GRANT The irreversible oxidation of cysteine residues can be prevented by protein S-thiolation, a process by which protein SH groups form mixed disulphides with low-molecular-mass thiols such as glutathione. We report here the target proteins which are modified in yeast...
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Biochem J (2003) 373 (3): 793–803.
Published: 01 August 2003
... To whom correspondence should be addressed (e-mail ysaito@bio.titech.ac.jp ). 10 7 2002 2 5 2003 9 5 2003 9 5 2003 The Biochemical Society, London ©2003 2003 cytosol disulphide glutathione nucleotide ribonuclease A Abbreviations used: tTG, tissue-type...