...Sebastian Brings; Walter Mier; Barbro Beijer; Elisabeth Kliemank; Stephan Herzig; Julia Szendroedi; Peter P. Nawroth; Thomas Fleming Advanced glycation end products (AGEs) are non-enzymatic post-translational modifications of amino acids and are associated with diabetic complications. One proposed...

... and the Biochemical Society under a transformative agreement with Individual. acetylation/deacetylation acylation glycation mass spectrometry methylglyoxal post-translational modification The ability of cells to rapidly detect and respond to stimuli is critical to maintaining homeostasis [ 1 , 2...

... photoproducts are predicted to include four- and five-carbon sugars linked to ADP. These FAD photoproducts were shown to be potent glycating agents, more so than ADP-ribose. Such toxic compounds would require disposal via an ADP-sugar diphosphatase or other route. Comparative analysis of bacterial genomes...

... are glycated by methylglyoxal. Even though no consensus has been reached about the actual role of methylglyoxal-derived advanced glycation end-products in amyloid diseases, evidence collected so far points to a role for protein glycation in conformational abnormalities, being ubiquitously found in amyloid...

... with protein amino groups, known as glycation, has been shown to compromise the action of Tf (transferrin), the systemic iron transporter. In order to understand the structural alterations that impair its function, Tf was glycated in vitro and the modification sites were determined by MS. Iron binding...

... designation prevailed. However, in 1951 it was shown that two enzymes were needed and that glutathione was the required catalytic co-factor. The concept of a metabolic pathway defined by two enzymes emerged at this time. Its association to detoxification and anti-glycation defence are its presently accepted...

... glycation glyoxalase methylglyoxal Nrf2 protein damage Modification of proteins and DNA by the dicarbonyl metabolite MG (methylglyoxal) has emerged as an important endogenous threat to the functional integrity of the proteome and genome. MG is formed by the spontaneous degradation of triose...

...Leo S. Payne; Peter M. Brown; Martin Middleditch; Edward Baker; Garth J. S. Cooper; Kerry M. Loomes The modification of proteins by reducing sugars through the process of non-enzymatic glycation is one of the principal mechanisms by which hyperglycaemia may precipitate the development of diabetic...

...Juliette Fortpied; Rita Gemayel; Didier Vertommen; Emile Van Schaftingen Ribulosamines, which are substrates for the deglycating enzyme fructosamine-3-kinase-related protein, are presumably formed intracellularly through glycation of proteins with ribose 5-phosphate followed by dephosphorylation...

... should be addressed (email [email protected] ). 2 11 2006 31 1 2007 21 2 2007 21 2 2007 © The Authors Journal compilation © 2007 Biochemical Society 2007 glycation lysine methylglyoxal myeloperoxidase redox-active metals semicarbazide-sensitive amine oxidase...

... by fructose. 1 email [email protected] 5 10 2005 10 10 2005 The Biochemical Society, London 2005 advanced glycation end-product (AGE) diabetes fructoselysine glucoselysine glycation Maillard reaction Glycation is the process by which reducing sugars react non...

...Elsa Wiame; Pedro Lamosa; Helena Santos; Emile Van Schaftingen The metabolism of the glycation product fructose-ϵ-lysine in Escherichia coli involves its ATP-dependent phosphorylation by a specific kinase (FrlD), followed by the conversion of fructoselysine 6-phosphate into glucose 6-phosphate...

... (senile systemic amyloidosis), suggesting that mutations may only accelerate this process, but are not the determinant factor in amyloid fibril formation and cell toxicity. We propose that glycation is involved in amyloidogenesis, since amyloid fibrils present several properties common to glycated...

... of FN3K), penetrated erythrocytes and was converted into the corresponding 3-phospho-derivative. Incubation of erythrocytes with 50 mM allose, 200 mM glucose or 10 mM ribose for 24 h resulted in the accumulation of glycated haemoglobin, and this accumulation was approx. 1.9–2.6-fold higher if DMP...

... fructosamine fructoselysine glycation osamine psicosamine Abbreviations used: ORF, open reading frame; N -α-t-Boc-lysine, N -α-t-butoxycarbonyl-lysine. Biochem. J. (2004) 378, 1047 1052 (Printed in Great Britain) 1047 Fructoselysine 3-epimerase, an enzyme involved in the metabolism...

...Hong YAN; Antony C. WILLIS; John J. HARDING Several mechanisms have been proposed for the way in which glucose and its metabolites cause cataract, retinopathy and other complications of diabetes, the most convincing being glycation. Glycation, the reaction of sugars with free amino groups...

... of the Maillard reaction is the formation of covalently cross-linked [2 6] and non-cross- linked [7 10] amino acids, many of which are chromophores or fluorophores. The reaction is initiated by non-enzymic glycosyl- ation, also called early-glycation, between primary amino groups in proteins and reducing sugars...

... removing fructosamine residues from proteins. In the present study, we show that incubation of human erythrocytes with 200mM glucose not only caused the progressive formation of glycated haemoglobin, but also increased the level of an anionic form of haemoglobin containing alkali-labile phosphate...

...Naila AHMED; Paul J. THORNALLEY Glycation of proteins leads to the formation of advanced glycation endproducts (AGEs) of diverse molecular structure and biological function. Serum albumin derivatives modified to minimal and high extents by methylglyoxal and glucose in vitro have been used in many...

...-phosphate glycation NMR phosphate esters Biochem. J. (2000) 352, 835 839 (Printed in Great Britain) 835 Conversion of a synthetic fructosamine into its 3-phospho derivative in human erythrocytes Ghislain DELPIERRE*, Florent VANSTAPEL‹, Vincent STROOBANTŒ and Emile VAN SCHAFTINGEN*1 *Laboratory...

... of reaction with reducing sugars or their oxidation products [3,6,7]. The reaction with reducing sugars can generate protein carbonyls as ketoamines derived from early glycation reactions and by more complex glycoxidation reactions that lead to the generation of fluorescent advanced glycation end products...