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Keywords: glycolysis
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Articles
Biochem J (2021) BCJ20200975.
Published: 12 April 2021
... expression of the polyP hydrolyzing enzyme (scPPX). We found that these cells have significantly reduced rates of oxidative phosphorylation (OXPHOS), while their rates of glycolysis were elevated. Consistent with this, metabolomics assays confirmed increased levels of metabolites involved in glycolysis in...
Articles
Biochem J (2020) 477 (10): 1795–1811.
Published: 21 May 2020
...Tomokazu Ohnishi; Joji Kusuyama; Kenjiro Bandow; Tetsuya Matsuguchi The glycolytic system is selected for ATP synthesis not only in tumor cells but also in differentiated cells. Differentiated osteoblasts also switch the dominant metabolic pathway to aerobic glycolysis. We found that primary...
Includes: Supplementary data
Articles
Biochem J (2020) 477 (9): 1733–1744.
Published: 15 May 2020
.... Interestingly, glycolytic enzymes were more acetylated in the procyclic, which develops in insects and uses oxidative phosphorylation to obtain energy, compared with the bloodstream form, whose main source of energy is glycolysis. Here, we investigated whether acetylation regulates the T. brucei fructose 1,6...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (12): 1713–1724.
Published: 19 June 2019
... glucose utilization and impaired fatty acid use. We demonstrate that glycolysis is uncoupled from glucose oxidation under normoxic conditions in GCN5L1-depleted cells. We show that GCN5L1 directly binds to the Akt-activating mTORC2 component Rictor, and that loss of Rictor acetylation is evident in GCN5L1...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (10): 1497–1513.
Published: 28 May 2019
... rely heavily on rapid anaerobic glycolysis for energy production. This switch from oxidative phosphorylation to glycolysis, along with up-regulation of the glucose transport system, significantly increases the release of lactic acid from cells into the tumor microenvironment. Excess lactate and proton...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (7): 1053–1082.
Published: 04 April 2019
... © 2019 The Author(s) 2019 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . amino acid metabolism comparative proteomics glycolysis maf1 RNA polymerase...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (4): 629–643.
Published: 19 February 2019
... Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . DNA damage response glycolysis metabolic regulation Phosphoglycolate can be formed in all organisms during the repair of DNA damage. Oxidative stress or treatment with...
Includes: Supplementary data
Articles
Biochem J (2018) 475 (16): 2577–2592.
Published: 29 August 2018
... evolution. As a consequence, it was frequently assumed that the topological organisation of the metabolic pathway has formed in a Darwinian process. The situation changed with the discovery of a non-enzymatic glycolysis and pentose phosphate pathway. The suite of metabolism-like reactions is promoted by a...
Articles
Biochem J (2018) 475 (14): 2355–2376.
Published: 31 July 2018
... glycolysis (lactic acid fermentation) and decreased the l -lactate/ d -glucose ratio (also termed as the Warburg effect) in normal and cancer cells. Overall, our findings implied that because of its interactions with VDAC-1, the cryptic MBP84-104 peptide invoked reprogramming of the cellular energy...
Includes: Supplementary data
Articles
Biochem J (2017) 474 (23): 3935–3950.
Published: 16 November 2017
... energetic resources such as ATP and NADPH. In this review, we explore these strategies, focusing on key metabolic pathways and processes, such as glycolysis, anaplerosis, the TCA (tricarboxylic acid) cycle, and NADPH production. We show that only a holistic approach for bioengineering — considering the...
Articles
Biochem J (2017) 474 (16): 2785–2801.
Published: 07 August 2017
... the present study, we used radiometric glycolysis assays, [ 13 C 6 ]-glucose isotope tracing, and extracellular flux analysis to understand how phosphofructokinase (PFK)-mediated changes in glycolysis regulate glucose carbon partitioning into catabolic and anabolic pathways. Expression of kinase...
Articles
Biochem J (2015) 469 (3): 421–432.
Published: 23 July 2015
...Marco Kloos; Antje Brüser; Jürgen Kirchberger; Torsten Schöneberg; Norbert Sträter Phosphofructokinase-1 (Pfk) acts as the main control point of flux through glycolysis. It is involved in complex allosteric regulation and Pfk mutations have been linked to cancer development. Whereas the 3D...
Includes: Supplementary data
Articles
Biochem J (2015) 467 (2): 303–310.
Published: 02 April 2015
... (phosphatase and tensin homologue deleted on chromosome 10-induced protein kinase-1) gene. Moreover, we demonstrate that, by promoting pink1 expression, DJ1 represses the rate of glycolysis and cell proliferation. 1 These authors equally contributed to this work. 2 To whom correspondence should...
Articles
Biochem J (2015) 465 (1): 49–61.
Published: 12 December 2014
... differences in basal bioenergetics profiles and bioenergetics responses to serum depletion, oestradiol and tamoxifen as measured in real time by extracellular flux analysis in intact cells. breast cancer glycolysis extracellular flux mitochondrial function oxygen consumption rate respiration...
Includes: Supplementary data
Articles
Biochem J (2014) 464 (1): 35–48.
Published: 23 October 2014
... pyruvate to acetyl-CoA for entry into the Krebs cycle; in the absence of MondoA, pyruvate is diverted towards lactate and alanine, both products of glycolysis. Dynamic testing revealed that MondoA −/− mice excel in sprinting as their skeletal muscles display an enhanced glycolytic capacity. Our studies...
Includes: Supplementary data
Articles
Biochem J (2014) 458 (3): 439–448.
Published: 28 February 2014
...Isabelle Gerin; Gaëtane Noël; Jennifer Bolsée; Olivier Haumont; Emile Van Schaftingen; Guido T. Bommer The p53-induced protein TIGAR [TP53 (tumour protein 53)-induced glycolysis and apoptosis regulator] is considered to be a F26BPase (fructose-2,6-bisphosphatase) with an important role in cancer...
Articles
Biochem J (2014) 458 (3): e5–e7.
Published: 28 February 2014
...Juan P. Bolaños TIGAR [TP53 (tumour protein 53)-induced glycolysis and apoptosis regulator] protein is known for its ability to inhibit glycolysis, shifting glucose consumption towards the pentose phosphate pathway to promote antioxidant protection of cancer cells. According to sequence homology...
Articles
Biochem J (2013) 454 (2): 227–237.
Published: 09 August 2013
... Authors Journal compilation © 2013 Biochemical Society 2013 fermentation glycolysis hexokinase metabolic regulation trehalose yeast The yeast Saccharomyces cerevisiae is currently the most frequently used organism for the production of bioethanol; however, despite its wide use as an...
Includes: Supplementary data
Articles
Biochem J (2013) 452 (3): e7–e9.
Published: 31 May 2013
...Juan P. Bolaños Besides the necessary changes in the expression of cell cycle-related proteins, cancer cells undergo a profound series of metabolic adaptations focused to satisfy their excessive demand for biomass. An essential metabolic transformation of these cells is increased glycolysis, which...
Articles
Biochem J (2013) 452 (3): 531–543.
Published: 31 May 2013
...-bisphosphate), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is involved in cell proliferation owing to its role in carbohydrate metabolism. In the present study we analysed the mechanism of regulation of PFKFB3 as an immediate early gene controlled by stress stimuli that activates the p38...
Includes: Supplementary data
Articles
Biochem J (2012) 448 (1): 165–169.
Published: 18 October 2012
...-cells increase following BCR engagement and are characterized by a metabolic switch to aerobic glycolysis; however, the signalling pathways involved in this metabolic reprogramming are poorly defined. The PKC (protein kinase C) family plays an integral role in B-cell survival and antigenic responses...
Includes: Supplementary data
Articles
Biochem J (2012) 445 (3): 337–347.
Published: 13 July 2012
... cytosolic glutaredoxin, via a GSH-dependent monothiol mechanism, or, less efficiently, by cytosolic thioredoxins physiologically reduced by NADPH:thioredoxin reductase. The potential relevance of these findings is discussed in the light of the multiple functions of GAPDH in eukaryotic cells (e.g. glycolysis...
Includes: Supplementary data
Articles
Biochem J (2012) 445 (2): 213–218.
Published: 27 June 2012
...Oscar H. Martínez-Costa; Valentina Sánchez; Antonio Lázaro; Eloy D. Hernández; Keith Tornheim; Juan J. Aragón Eukaryotic PFK (phosphofructokinase), a key regulatory enzyme in glycolysis, has homologous N- and C-terminal domains thought to result from duplication, fusion and divergence of an...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (3): 537–551.
Published: 29 May 2012
... of 13 C-labelled glucose in both a liver perfusion system and isolated hepatocytes. MS and NMR spectroscopy were deployed to measure isotopic enrichment. The results demonstrate that the stimulation of glycolysis by GKA led to numerous changes in hepatic metabolism: (i) augmented flux through the TCA...
Includes: Supplementary data
Articles
Biochem J (2012) 444 (2): 249–259.
Published: 11 May 2012
... mimicked by the activation of AMPK and the silencing of ATF4 (activating transcription factor 4). These findings emphasize the relevance of translational control for normal mitochondrial function and for the progression of cancer. Moreover, they demonstrate that glycolysis and oxidative phosphorylation are...
Articles
Biochem J (2012) 443 (1): 3–11.
Published: 14 March 2012
..., which maintains the antioxidant glutathione in its reduced state, hence exerting neuroprotection. This process is tightly controlled by a key glycolysis-promoting enzyme and is dependent on an appropriate supply of energy substrates from astrocytes. Thus brain bioenergetic and antioxidant defence is...
Includes: Multimedia, Supplementary data
Articles
Biochem J (2012) 442 (2): 345–356.
Published: 13 February 2012
...), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is extensively involved in cell proliferation owing to its key role in carbohydrate metabolism. In the present study we analyse its mechanism of regulation by progestins in breast cancer cells. We report that exposure of T47D cells...
Articles
Biochem J (2011) 440 (2): 229–240.
Published: 14 November 2011
.... These results implicate PP i as having a significant regulatory role in glycolysis and, potentially, other downstream processes that regulate growth and cell division. PP i is a byproduct of many biosynthetic reactions (the synthesis of nucleic acids, coenzymes, proteins and isoprenoids, and the...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): 519–528.
Published: 29 March 2011
... of oxygen consumption and a 2-fold increase in glycolysis upon HNE exposure. This augmentation of glycolytic flux was not due to increased glucose uptake, suggesting that electrophile stress results in utilization of intracellular glycogen stores to support the increased energy demand. Hypertrophied...
Includes: Supplementary data
Articles
Biochem J (2011) 433 (1): 225–233.
Published: 15 December 2010
... compilation © 2011 Biochemical Society 2011 androgen glycolysis hexokinase 2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (PFKFB2) prostate cancer Prostate cancer is the most common cancer in males, where androgen is a critical factor in regulating cell proliferation and growth...
Includes: Supplementary data
Articles
Biochem J (2010) 431 (2): 267–275.
Published: 28 September 2010
...@uclouvain.be ). 19 7 2010 5 8 2010 5 8 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 glycolysis protein kinase B (PKB) 6-phosphofructo-2-kinase (PFK-2) serum- and glucocorticoid-induced protein kinase 3 (SGK3) small interfering RNA (siRNA...
Includes: Supplementary data
Articles
Biochem J (2009) 424 (1): 99–107.
Published: 23 October 2009
... cardiomyocyte extracellular flux glycolysis heart lipid peroxidation mitochondrion oxidative stress Defining the role of mitochondria in various cardiovascular pathologies is currently an area of great interest, with many studies focusing on the properties of the organelle isolated from diseased...
Articles
Biochem J (2009) 420 (2): 161–168.
Published: 13 May 2009
...Niels Ørtenblad; Will A. Macdonald; Kent Sahlin The control of glycolysis in contracting muscle is not fully understood. The aim of the present study was to examine whether activation of glycolysis is mediated by factors related to the energy state or by a direct effect of Ca 2+ on the regulating...
Articles
Biochem J (2009) 417 (3): 717–726.
Published: 16 January 2009
... cellular ATP production owing to its action on glycolysis and oxidative phosphorylation; however, the specific metabolic steps and mechanisms of 3-BrPA action in human hepatocellular carcinomas, particularly its effects on mitochondrial energetics, are poorly understood. In the present study it was found...
Articles
Biochem J (2007) 408 (1): 123–130.
Published: 29 October 2007
...Tiago Costa Leite; Daniel Da Silva; Raquel Guimarães Coelho; Patricia Zancan; Mauro Sola-Penna For a long period lactate was considered as a dead-end product of glycolysis in many cells and its accumulation correlated with acidosis and cellular and tissue damage. At present, the role of lactate in...
Articles
Biochem J (2006) 400 (1): 143–151.
Published: 27 October 2006
... protein interaction cytoplasmic domain of band 3 erythrocyte cytoskeleton erythrocyte membrane structure glycolysis glycolytic enzyme complex The GEs (glycolytic enzymes) GAPDH (glyceraldehyde-3-phosphate dehydrogenase), aldolase, PFK (phosphofructokinase), LDH (lactate dehydrogenase) and PK...
Articles
Biochem J (2006) 396 (2): 317–326.
Published: 15 May 2006
... of glycolysis reconstituted in vitro , we showed how to derive, from titration experiments, values of effective enzyme activity parameters that do not include explicitly any of the classical kinetic constants. With a maximum of only two parameters per enzyme, this approach produced very good...
Articles
Biochem J (2006) 393 (1): 431–439.
Published: 12 December 2005
... the variable values observed with the 15 controls. Our observations suggest that, when glycolysis and mitochondria generate ATP, and in the absence of appropriate activators of proton transport, UCPs do not transport protons (uncoupling), but rather other ions of physiological relevance that control...
Articles
Biochem J (2005) 392 (3): 675–683.
Published: 06 December 2005
... mutations (F240L and E145Stop), but only the younger one suffers from neurodegeneration. In the present study, we determined the kinetic parameters of key glycolytic enzymes including the mutant TPI for rational modelling of erythrocyte glycolysis. We found that a low TPI activity in the mutant cells (lower...
Includes: Supplementary data
Articles
Biochem J (2004) 384 (3): 629–636.
Published: 07 December 2004
...Pilar CIDAD; Angeles ALMEIDA; Juan P. BOLAÑOS Recently, we have reported that the inhibition of mitochondrial respiration by nitric oxide (NO) leads to an up-regulation of glycolysis and affords cytoprotection against energy failure through the stimulation of AMPK (5′-AMP-activated protein kinase...
Articles
Biochem J (2004) 381 (3): 561–579.
Published: 27 July 2004
...Mark H. RIDER; Luc BERTRAND; Didier VERTOMMEN; Paul A. MICHELS; Guy G. ROUSSEAU; Louis HUE Fru-2,6-P 2 (fructose 2,6-bisphosphate) is a signal molecule that controls glycolysis. Since its discovery more than 20 years ago, inroads have been made towards the understanding of the structure–function...
Articles
Biochem J (2004) 378 (1): 17–20.
Published: 15 February 2004
... 19 12 2003 The Biochemical Society, London ©2004 2004 cancer glycolysis H + -ATP synthase mitochondria Abbreviations used: β-F 1 -ATPase, β-subunit of the mitochondrial H + -ATP synthase; BEC index, bioenergetic cellular index; GAPDH, glyceraldehyde-3-phosphate dehydrogenase...
Articles
Biochem J (2004) 377 (1): 77–84.
Published: 01 January 2004
... whom correspondence should be addressed (e-mail juanjose.aragon@uam.es ). 9 7 2003 2 9 2003 16 9 2003 16 9 2003 The Biochemical Society, London ©2004 2004 allosteric regulation allosteric transition enzyme engineering enzyme regulation glycolysis...
Articles
Biochem J (2003) 376 (2): 403–411.
Published: 01 December 2003
... premature senescence, reactive oxygen species are considered important intermediates contributing to the phenotype. Moreover, distinct alterations of the cellular carbohydrate metabolism are known to contribute to oncogenic transformation, as is best documented for the phenomenon of aerobic glycolysis...
Articles
Biochem J (2003) 375 (2): 231–246.
Published: 15 October 2003
... john.vanderoost@wur.nl ). 20 9 2002 16 5 2003 18 8 2003 18 8 2003 The Biochemical Society, London ©2003 2003 Archaea Embden—Meyerhof pathway Entner—Douderoff pathway glycolysis glycolytic enzyme polysaccharide Abbreviations used: AOR, aldehyde oxidoreductase; ED...
Articles
Biochem J (2003) 370 (2): 609–619.
Published: 01 March 2003
... 12 2002 The Biochemical Society, London ©2003 2003 ATPase 6-8 free radical glycolysis mitochondrial transcript necrosis tumour necrosis factor α (TNF-α) Abbreviations used: BHA, butylated hydroxianisole; BHT, butylated hydroxytoluene; COX II, cytochrome c oxidase, subunit...
Articles
Biochem J (2002) 368 (1): 283–291.
Published: 15 November 2002
... addressed (e-mail tauler@farmacia.far.ub.es ). 19 4 2002 16 7 2002 24 7 2002 24 7 2002 The Biochemical Society, London ©2002 2002 cell cycle E2F glycolysis Abbreviations used: BrdUrd, bromodeoxyuridine; cdk2, cyclin-dependent kinase 2; DCS, donor calf serum...
Articles
Biochem J (2002) 366 (1): 63–71.
Published: 15 August 2002
..., Morelos 62210, Mexico. 4 To whom correspondence should be addressed (e-mail johan.thevelein@bio.kuleuven.ac.be ). 3 4 2002 23 4 2002 29 4 2002 The Biochemical Society, London ©2002 2002 glycolysis Saccharomyces cerevisiae sugar influx Abbreviations used...
Articles
Biochem J (2002) 365 (1): 223–228.
Published: 01 July 2002
... 2002 12 4 2002 The Biochemical Society, London ©2002 2002 glycolysis hypoxia insulin maturity-onset diabetes of the young metabolic zonation nuclear receptor Abbreviations used: DIG, digoxigenin; EMSA, electrophoretic mobility-shift assay; EPO, erythropoietin; GK...
Articles
Biochem J (2001) 358 (2): 481–487.
Published: 24 August 2001
... and is accompanied by metabolic acidosis. Moreover, hypoxic cell death is inhibited by Hepes buffer as well as by 2-deoxyglucose, an inhibitor of glycolysis, indicating that metabolic acidosis should play an essential role in hypoxic injury. The involvement of phosphoinositide 3-kinase (PI 3-kinase...