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Keywords: glycolysis
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Articles

Cancer-associated somatic mutations in human phosphofructokinase-1 reveal a critical electrostatic interaction for allosteric regulation of enzyme activity

Maria A. Voronkova, Heather L. Hansen, Madison P. Cooper, Jacob Miller, Narayanasami Sukumar, Werner J. Geldenhuys, Aaron R. Robart, Bradley A. Webb
Journal: Biochemical Journal
Biochem J (2023) 480 (17): 1411–1427.
https://doi.org/10.1042/BCJ20230207
Published: 04 September 2023
... regulation. We showed that PFKP-D564N had a decreased total system energy and changes in the electrostatic surface potential of the effector site. Cells expressing PFKP-D564N demonstrated a decreased rate of glycolysis, while their ability to induce glycolytic flux under conditions of low cellular energy...
View articletitled, Cancer-associated somatic mutations in human phosphofructokinase-1 reveal a critical electrostatic interaction for allosteric regulation of enzyme activity
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Includes: Supplementary data
Articles

Depletion of mitochondrial inorganic polyphosphate (polyP) in mammalian cells causes metabolic shift from oxidative phosphorylation to glycolysis

Maria E. Solesio, Lihan Xie, Brendan McIntyre, Mathew Ellenberger, Erna Mitaishvili, Siddharth Bhadra-Lobo, Lisa F. Bettcher, Jason N. Bazil, Daniel Raftery, Ursula Jakob, Evgeny V. Pavlov
Journal: Biochemical Journal
Biochem J (2021) 478 (8): 1631–1646.
https://doi.org/10.1042/BCJ20200975
Published: 30 April 2021
... expression of the polyP hydrolyzing enzyme (scPPX). We found that these cells have significantly reduced rates of oxidative phosphorylation (OXPHOS), while their rates of glycolysis were elevated. Consistent with this, metabolomics assays confirmed increased levels of metabolites involved in glycolysis...
View articletitled, Depletion of mitochondrial inorganic polyphosphate (polyP) in mammalian cells causes metabolic shift from oxidative phosphorylation to glycolysis
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Includes: Supplementary data
Articles

Glut1 expression is increased by p53 reduction to switch metabolism to glycolysis during osteoblast differentiation

Tomokazu Ohnishi, Joji Kusuyama, Kenjiro Bandow, Tetsuya Matsuguchi
Journal: Biochemical Journal
Biochem J (2020) 477 (10): 1795–1811.
https://doi.org/10.1042/BCJ20190888
Published: 21 May 2020
...Tomokazu Ohnishi; Joji Kusuyama; Kenjiro Bandow; Tetsuya Matsuguchi The glycolytic system is selected for ATP synthesis not only in tumor cells but also in differentiated cells. Differentiated osteoblasts also switch the dominant metabolic pathway to aerobic glycolysis. We found that primary...
View articletitled, Glut1 expression is increased by p53 reduction to switch metabolism to glycolysis during osteoblast differentiation
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Includes: Supplementary data
Articles

Effect of lysine acetylation on the regulation of Trypanosoma brucei glycosomal aldolase activity

Ariely Barbosa Leite, Antoniel Augusto Severo Gomes, Ana Caroline de Castro Nascimento Sousa, Marcos Roberto de Mattos Fontes, Sergio Schenkman, Nilmar Silvio Moretti
Journal: Biochemical Journal
Biochem J (2020) 477 (9): 1733–1744.
https://doi.org/10.1042/BCJ20200142
Published: 15 May 2020
.... Interestingly, glycolytic enzymes were more acetylated in the procyclic, which develops in insects and uses oxidative phosphorylation to obtain energy, compared with the bloodstream form, whose main source of energy is glycolysis. Here, we investigated whether acetylation regulates the T. brucei fructose 1,6...
View articletitled, Effect of lysine acetylation on the regulation of Trypanosoma brucei glycosomal aldolase activity
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Includes: Supplementary data
Articles

Loss of GCN5L1 in cardiac cells disrupts glucose metabolism and promotes cell death via reduced Akt/mTORC2 signaling

Janet R. Manning, Dharendra Thapa, Manling Zhang, Michael W. Stoner, Javier Traba, Catherine Corey, Sruti Shiva, Michael N. Sack, Iain Scott
Journal: Biochemical Journal
Biochem J (2019) 476 (12): 1713–1724.
https://doi.org/10.1042/BCJ20190302
Published: 19 June 2019
... glucose utilization and impaired fatty acid use. We demonstrate that glycolysis is uncoupled from glucose oxidation under normoxic conditions in GCN5L1-depleted cells. We show that GCN5L1 directly binds to the Akt-activating mTORC2 component Rictor, and that loss of Rictor acetylation is evident in GCN5L1...
View articletitled, Loss of GCN5L1 in cardiac cells disrupts glucose metabolism and promotes cell death via reduced Akt/mTORC2 signaling
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Includes: Supplementary data
Articles

A non-catalytic function of carbonic anhydrase IX contributes to the glycolytic phenotype and pH regulation in human breast cancer cells

In Collection
Editors' pick of research highlights published in the Biochemical Journal in 2018 & 2019
Mam Y. Mboge, Zhijuan Chen, Daniel Khokhar, Alyssa Wolff, Lingbao Ai, Coy D. Heldermon, Murat Bozdag, Fabrizio Carta, Claudiu T. Supuran, Kevin D. Brown, Robert McKenna, Christopher J. Frost, Susan C. Frost
Journal: Biochemical Journal
Biochem J (2019) 476 (10): 1497–1513.
https://doi.org/10.1042/BCJ20190177
Published: 28 May 2019
... and rely heavily on rapid anaerobic glycolysis for energy production. This switch from oxidative phosphorylation to glycolysis, along with up-regulation of the glucose transport system, significantly increases the release of lactic acid from cells into the tumor microenvironment. Excess lactate and proton...
View articletitled, A non-catalytic function of carbonic anhydrase IX contributes to the glycolytic phenotype and pH regulation in human breast cancer cells
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Includes: Supplementary data
Articles

Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1

Roza Szatkowska, Manuel Garcia-Albornoz, Katarzyna Roszkowska, Stephen W. Holman, Emil Furmanek, Simon J. Hubbard, Robert J. Beynon, Malgorzata Adamczyk
Journal: Biochemical Journal
Biochem J (2019) 476 (7): 1053–1082.
https://doi.org/10.1042/BCJ20180701
Published: 04 April 2019
...) with an ion selection window of 3  m/z and a normalized collision energy of 30. To avoid repeated selection of peptides for MS/MS the program used a 30 s dynamic exclusion window. amino acid metabolism comparative proteomics glycolysis maf1 RNA polymerase III Regulation of glycolytic flux...
View articletitled, Glycolytic flux in Saccharomyces cerevisiae is dependent on RNA polymerase III and its negative regulator Maf1
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Includes: Supplementary data
Articles

Phosphoglycolate has profound metabolic effects but most likely no role in a metabolic DNA response in cancer cell lines

Isabelle Gerin, Marina Bury, Francesca Baldin, Julie Graff, Emile Van Schaftingen, Guido T. Bommer
Journal: Biochemical Journal
Biochem J (2019) 476 (4): 629–643.
https://doi.org/10.1042/BCJ20180435
Published: 19 February 2019
... in cells, what metabolic changes its accumulation can provoke and whether phosphoglycolate released during DNA repair might lead to noticeable metabolic changes. DNA damage response glycolysis metabolic regulation Phosphoglycolate can be formed in all organisms during the repair of DNA...
View articletitled, Phosphoglycolate has profound metabolic effects but most likely no role in a metabolic DNA response in cancer cell lines
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Includes: Supplementary data
Articles

An appeal to magic? The discovery of a non-enzymatic metabolism and its role in the origins of life

In Collection
Awards , Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019
Markus Ralser
Journal: Biochemical Journal
Biochem J (2018) 475 (16): 2577–2592.
https://doi.org/10.1042/BCJ20160866
Published: 29 August 2018
... evolution. As a consequence, it was frequently assumed that the topological organisation of the metabolic pathway has formed in a Darwinian process. The situation changed with the discovery of a non-enzymatic glycolysis and pentose phosphate pathway. The suite of metabolism-like reactions is promoted...
View articletitled, An appeal to magic? The discovery of a non-enzymatic metabolism and its role in the origins of life
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Articles

Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism

Albert G. Remacle, Swathi K. Hullugundi, Jennifer Dolkas, Mila Angert, Piotr Cieplak, David Scott, Andrei V. Chernov, Veronica I. Shubayev, Alex Y. Strongin
Journal: Biochemical Journal
Biochem J (2018) 475 (14): 2355–2376.
https://doi.org/10.1042/BCJ20180137
Published: 31 July 2018
... glycolysis (lactic acid fermentation) and decreased the l -lactate/ d -glucose ratio (also termed as the Warburg effect) in normal and cancer cells. Overall, our findings implied that because of its interactions with VDAC-1, the cryptic MBP84-104 peptide invoked reprogramming of the cellular energy...
View articletitled, Interaction of the cryptic fragment of myelin basic protein with mitochondrial voltage-dependent anion-selective channel-1 affects cell energy metabolism
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Includes: Supplementary data
Articles

Holistic bioengineering: rewiring central metabolism for enhanced bioproduction

Selçuk Aslan, Elad Noor, Arren Bar-Even
Journal: Biochemical Journal
Biochem J (2017) 474 (23): 3935–3950.
https://doi.org/10.1042/BCJ20170377
Published: 16 November 2017
... of energetic resources such as ATP and NADPH. In this review, we explore these strategies, focusing on key metabolic pathways and processes, such as glycolysis, anaplerosis, the TCA (tricarboxylic acid) cycle, and NADPH production. We show that only a holistic approach for bioengineering — considering...
View articletitled, Holistic bioengineering: rewiring central metabolism for enhanced bioproduction
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Articles

Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes

Andrew A. Gibb, Pawel K. Lorkiewicz, Yu-Ting Zheng, Xiang Zhang, Aruni Bhatnagar, Steven P. Jones, Bradford G. Hill
Journal: Biochemical Journal
Biochem J (2017) 474 (16): 2785–2801.
https://doi.org/10.1042/BCJ20170474
Published: 07 August 2017
.... In the present study, we used radiometric glycolysis assays, [ 13 C 6 ]-glucose isotope tracing, and extracellular flux analysis to understand how phosphofructokinase (PFK)-mediated changes in glycolysis regulate glucose carbon partitioning into catabolic and anabolic pathways. Expression of kinase-deficient...
View articletitled, Integration of flux measurements to resolve changes in anabolic and catabolic metabolism in cardiac myocytes
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Articles

Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation

Marco Kloos, Antje Brüser, Jürgen Kirchberger, Torsten Schöneberg, Norbert Sträter
Journal: Biochemical Journal
Biochem J (2015) 469 (3): 421–432.
https://doi.org/10.1042/BJ20150251
Published: 23 July 2015
...Marco Kloos; Antje Brüser; Jürgen Kirchberger; Torsten Schöneberg; Norbert Sträter Phosphofructokinase-1 (Pfk) acts as the main control point of flux through glycolysis. It is involved in complex allosteric regulation and Pfk mutations have been linked to cancer development. Whereas the 3D...
View articletitled, Crystal structure of human platelet phosphofructokinase-1 locked in an activated conformation
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Articles

DJ1 represses glycolysis and cell proliferation by transcriptionally up-regulating pink1

Raquel Requejo-Aguilar, Irene Lopez-Fabuel, Daniel Jimenez-Blasco, Emilio Fernandez, Angeles Almeida, Juan P. Bolaños
Journal: Biochemical Journal
Biochem J (2015) 467 (2): 303–310.
https://doi.org/10.1042/BJ20141025
Published: 02 April 2015
... (phosphatase and tensin homologue deleted on chromosome 10-induced protein kinase-1) gene. Moreover, we demonstrate that, by promoting pink1 expression, DJ1 represses the rate of glycolysis and cell proliferation. 1 These authors equally contributed to this work. 2 To whom correspondence should...
View articletitled, DJ1 represses glycolysis and cell proliferation by transcriptionally up-regulating pink1
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Articles

Bioenergetic differences between MCF-7 and T47D breast cancer cells and their regulation by oestradiol and tamoxifen

Brandie N. Radde, Margarita M. Ivanova, Huy Xuan Mai, Joshua K. Salabei, Bradford G. Hill, Carolyn M. Klinge
Journal: Biochemical Journal
Biochem J (2015) 465 (1): 49–61.
https://doi.org/10.1042/BJ20131608
Published: 12 December 2014
... cancer cells show differences in basal bioenergetics profiles and bioenergetics responses to serum depletion, oestradiol and tamoxifen as measured in real time by extracellular flux analysis in intact cells. breast cancer glycolysis extracellular flux mitochondrial function oxygen consumption...
View articletitled, Bioenergetic differences between MCF-7 and T47D breast cancer cells and their regulation by oestradiol and tamoxifen
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Includes: Supplementary data
Articles

MondoA deficiency enhances sprint performance in mice

Minako Imamura, Benny Hung-Junn Chang, Motoyuki Kohjima, Ming Li, Byounghoon Hwang, Heinrich Taegtmeyer, Robert A. Harris, Lawrence Chan
Journal: Biochemical Journal
Biochem J (2014) 464 (1): 35–48.
https://doi.org/10.1042/BJ20140530
Published: 23 October 2014
... to acetyl-CoA for entry into the Krebs cycle; in the absence of MondoA, pyruvate is diverted towards lactate and alanine, both products of glycolysis. Dynamic testing revealed that MondoA −/− mice excel in sprinting as their skeletal muscles display an enhanced glycolytic capacity. Our studies uncover...
View articletitled, MondoA deficiency enhances sprint performance in mice
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Articles

TIGAR's promiscuity

Juan P. Bolaños
Journal: Biochemical Journal
Biochem J (2014) 458 (3): e5–e7.
https://doi.org/10.1042/BJ20140087
Published: 28 February 2014
...Juan P. Bolaños TIGAR [TP53 (tumour protein 53)-induced glycolysis and apoptosis regulator] protein is known for its ability to inhibit glycolysis, shifting glucose consumption towards the pentose phosphate pathway to promote antioxidant protection of cancer cells. According to sequence homology...
View articletitled, TIGAR's promiscuity
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Articles

Identification of TP53-induced glycolysis and apoptosis regulator (TIGAR) as the phosphoglycolate-independent 2,3-bisphosphoglycerate phosphatase

Isabelle Gerin, Gaëtane Noël, Jennifer Bolsée, Olivier Haumont, Emile Van Schaftingen, Guido T. Bommer
Journal: Biochemical Journal
Biochem J (2014) 458 (3): 439–448.
https://doi.org/10.1042/BJ20130841
Published: 28 February 2014
...Isabelle Gerin; Gaëtane Noël; Jennifer Bolsée; Olivier Haumont; Emile Van Schaftingen; Guido T. Bommer The p53-induced protein TIGAR [TP53 (tumour protein 53)-induced glycolysis and apoptosis regulator] is considered to be a F26BPase (fructose-2,6-bisphosphatase) with an important role in cancer...
View articletitled, Identification of TP53-induced glycolysis and apoptosis regulator (TIGAR) as the phosphoglycolate-independent 2,3-bisphosphoglycerate phosphatase
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Articles

Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics

Thomas Walther, Narjes Mtimet, Ceren Alkim, Amélie Vax, Marie-Odile Loret, Azmat Ullah, Carlos Gancedo, Gertien J. Smits, Jean Marie François
Journal: Biochemical Journal
Biochem J (2013) 454 (2): 227–237.
https://doi.org/10.1042/BJ20130587
Published: 09 August 2013
... to be completely unrelated to glucose metabolism and the function of hexokinase. Taken together, these results indicate that our understanding of Tps1/T6P function is incomplete and requires further analysis. fermentation glycolysis hexokinase metabolic regulation trehalose yeast The yeast...
View articletitled, Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics
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Includes: Supplementary data
Articles

Adapting glycolysis to cancer cell proliferation: the MAPK pathway focuses on PFKFB3

Juan P. Bolaños
Journal: Biochemical Journal
Biochem J (2013) 452 (3): e7–e9.
https://doi.org/10.1042/BJ20130560
Published: 31 May 2013
...Juan P. Bolaños Besides the necessary changes in the expression of cell cycle-related proteins, cancer cells undergo a profound series of metabolic adaptations focused to satisfy their excessive demand for biomass. An essential metabolic transformation of these cells is increased glycolysis, which...
View articletitled, Adapting glycolysis to cancer cell proliferation: the MAPK pathway focuses on PFKFB3
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Articles

PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress stimuli

Laura Novellasdemunt, Laurent Bultot, Anna Manzano, Francesc Ventura, Jose Luis Rosa, Didier Vertommen, Mark H. Rider, Àurea Navarro-Sabate, Ramon Bartrons
Journal: Biochemical Journal
Biochem J (2013) 452 (3): 531–543.
https://doi.org/10.1042/BJ20121886
Published: 31 May 2013
...-bisphosphate), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is involved in cell proliferation owing to its role in carbohydrate metabolism. In the present study we analysed the mechanism of regulation of PFKFB3 as an immediate early gene controlled by stress stimuli that activates the p38...
View articletitled, PFKFB3 activation in cancer cells by the p38/MK2 pathway in response to stress stimuli
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Articles

Protein kinase Cβ is critical for the metabolic switch to glycolysis following B-cell antigen receptor engagement

Derek Blair, Fay J. Dufort, Thomas C. Chiles
Journal: Biochemical Journal
Biochem J (2012) 448 (1): 165–169.
https://doi.org/10.1042/BJ20121225
Published: 18 October 2012
...-cells increase following BCR engagement and are characterized by a metabolic switch to aerobic glycolysis; however, the signalling pathways involved in this metabolic reprogramming are poorly defined. The PKC (protein kinase C) family plays an integral role in B-cell survival and antigenic responses...
View articletitled, Protein kinase Cβ is critical for the metabolic switch to glycolysis following B-cell antigen receptor engagement
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Articles

Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro

Mariette Bedhomme, Mattia Adamo, Christophe H. Marchand, Jérémy Couturier, Nicolas Rouhier, Stéphane D. Lemaire, Mirko Zaffagnini, Paolo Trost
Journal: Biochemical Journal
Biochem J (2012) 445 (3): 337–347.
https://doi.org/10.1042/BJ20120505
Published: 13 July 2012
... glutaredoxin, via a GSH-dependent monothiol mechanism, or, less efficiently, by cytosolic thioredoxins physiologically reduced by NADPH:thioredoxin reductase. The potential relevance of these findings is discussed in the light of the multiple functions of GAPDH in eukaryotic cells (e.g. glycolysis, control...
View articletitled, Glutathionylation of cytosolic glyceraldehyde-3-phosphate dehydrogenase from the model plant Arabidopsis thaliana is reversed by both glutaredoxins and thioredoxins in vitro
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Articles

Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase

Oscar H. Martínez-Costa, Valentina Sánchez, Antonio Lázaro, Eloy D. Hernández, Keith Tornheim, Juan J. Aragón
Journal: Biochemical Journal
Biochem J (2012) 445 (2): 213–218.
https://doi.org/10.1042/BJ20120173
Published: 27 June 2012
...Oscar H. Martínez-Costa; Valentina Sánchez; Antonio Lázaro; Eloy D. Hernández; Keith Tornheim; Juan J. Aragón Eukaryotic PFK (phosphofructokinase), a key regulatory enzyme in glycolysis, has homologous N- and C-terminal domains thought to result from duplication, fusion and divergence...
View articletitled, Distinct functional roles of the two terminal halves of eukaryotic phosphofructokinase
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Articles

Effects of a glucokinase activator on hepatic intermediary metabolism: study with 13 C-isotopomer-based metabolomics

Itzhak Nissim, Oksana Horyn, Ilana Nissim, Yevgeny Daikhin, Suzanne L. Wehrli, Marc Yudkoff, Franz M. Matschinsky
Journal: Biochemical Journal
Biochem J (2012) 444 (3): 537–551.
https://doi.org/10.1042/BJ20120163
Published: 29 May 2012
... of 13 C-labelled glucose in both a liver perfusion system and isolated hepatocytes. MS and NMR spectroscopy were deployed to measure isotopic enrichment. The results demonstrate that the stimulation of glycolysis by GKA led to numerous changes in hepatic metabolism: (i) augmented flux through the TCA...
View articletitled, Effects of a glucokinase activator on hepatic intermediary metabolism: study with 13 C-isotopomer-based metabolomics
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Includes: Supplementary data
Articles

AMPK and GCN2–ATF4 signal the repression of mitochondria in colon cancer cells

Inmaculada Martínez-Reyes, María Sánchez-Aragó, José M. Cuezva
Journal: Biochemical Journal
Biochem J (2012) 444 (2): 249–259.
https://doi.org/10.1042/BJ20111829
Published: 11 May 2012
... by the activation of AMPK and the silencing of ATF4 (activating transcription factor 4). These findings emphasize the relevance of translational control for normal mitochondrial function and for the progression of cancer. Moreover, they demonstrate that glycolysis and oxidative phosphorylation are controlled...
View articletitled, AMPK and GCN2–ATF4 signal the repression of mitochondria in colon cancer cells
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Articles

Antioxidant and bioenergetic coupling between neurons and astrocytes

Seila Fernandez-Fernandez, Angeles Almeida, Juan P. Bolaños
Journal: Biochemical Journal
Biochem J (2012) 443 (1): 3–11.
https://doi.org/10.1042/BJ20111943
Published: 14 March 2012
..., which maintains the antioxidant glutathione in its reduced state, hence exerting neuroprotection. This process is tightly controlled by a key glycolysis-promoting enzyme and is dependent on an appropriate supply of energy substrates from astrocytes. Thus brain bioenergetic and antioxidant defence...
View articletitled, Antioxidant and bioenergetic coupling between neurons and astrocytes
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Articles

Progestins activate 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) in breast cancer cells

Laura Novellasdemunt, Mercè Obach, Lluís Millán-Ariño, Anna Manzano, Francesc Ventura, Jose Luis Rosa, Albert Jordan, Àurea Navarro-Sabate, Ramon Bartrons
Journal: Biochemical Journal
Biochem J (2012) 442 (2): 345–356.
https://doi.org/10.1042/BJ20111418
Published: 13 February 2012
...), a key modulator of glycolysis and gluconeogenesis. The PFKFB3 gene is extensively involved in cell proliferation owing to its key role in carbohydrate metabolism. In the present study we analyse its mechanism of regulation by progestins in breast cancer cells. We report that exposure of T47D cells...
View articletitled, Progestins activate 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) in breast cancer cells
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Articles

Overexpression of a cytosolic pyrophosphatase (TgPPase) reveals a regulatory role of PP i in glycolysis for Toxoplasma gondii

Douglas A. Pace, Jianmin Fang, Roxana Cintron, Melissa D. Docampo, Silvia N. J. Moreno
Journal: Biochemical Journal
Biochem J (2011) 440 (2): 229–240.
https://doi.org/10.1042/BJ20110641
Published: 14 November 2011
.... These results implicate PP i as having a significant regulatory role in glycolysis and, potentially, other downstream processes that regulate growth and cell division. © The Authors Journal compilation © 2011 Biochemical Society 2011 Apicomplexa cellular metabolism glycolysis parasite PP i...
View articletitled, Overexpression of a cytosolic pyrophosphatase (TgPPase) reveals a regulatory role of PP i in glycolysis for Toxoplasma gondii
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Articles

Responses of hypertrophied myocytes to reactive species: implications for glycolysis and electrophile metabolism

Brian E. Sansbury, Daniel W. Riggs, Robert E. Brainard, Joshua K. Salabei, Steven P. Jones, Bradford G. Hill
Journal: Biochemical Journal
Biochem J (2011) 435 (2): 519–528.
https://doi.org/10.1042/BJ20101390
Published: 29 March 2011
... of oxygen consumption and a 2-fold increase in glycolysis upon HNE exposure. This augmentation of glycolytic flux was not due to increased glucose uptake, suggesting that electrophile stress results in utilization of intracellular glycogen stores to support the increased energy demand. Hypertrophied...
View articletitled, Responses of hypertrophied myocytes to reactive species: implications for glycolysis and electrophile metabolism
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Articles

Androgen stimulates glycolysis for de novo lipid synthesis by increasing the activities of hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 in prostate cancer cells

Jong-Seok Moon, Won-Ji Jin, Jin-Hye Kwak, Hyo-Jeong Kim, Mi-Jin Yun, Jae-Woo KIM, Sahng Wook Park, Kyung-Sup Kim
Journal: Biochemical Journal
Biochem J (2011) 433 (1): 225–233.
https://doi.org/10.1042/BJ20101104
Published: 15 December 2010
... glycolysis hexokinase 2 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 (PFKFB2) prostate cancer Prostate cancer is the most common cancer in males, where androgen is a critical factor in regulating cell proliferation and growth. The most characteristic metabolic change by androgen...
View articletitled, Androgen stimulates glycolysis for de novo lipid synthesis by increasing the activities of hexokinase 2 and 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 2 in prostate cancer cells
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Includes: Supplementary data
Articles

Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3)

Véronique Mouton, Louise Toussaint, Didier Vertommen, Marie-Agnès Gueuning, Liliane Maisin, Xavier Havaux, Cossette Sanchez-Canedo, Luc Bertrand, Franck Dequiedt, Brian A. Hemmings, Louis Hue, Mark H. Rider
Journal: Biochemical Journal
Biochem J (2010) 431 (2): 267–275.
https://doi.org/10.1042/BJ20101089
Published: 28 September 2010
[email protected] ). 19 7 2010 5 8 2010 5 8 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 glycolysis protein kinase B (PKB) 6-phosphofructo-2-kinase (PFK-2) serum- and glucocorticoid-induced protein kinase 3 (SGK3) small interfering RNA (siRNA...
View articletitled, Heart 6-phosphofructo-2-kinase activation by insulin requires PKB (protein kinase B), but not SGK3 (serum- and glucocorticoid-induced protein kinase 3)
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Includes: Supplementary data
Articles

Importance of the bioenergetic reserve capacity in response to cardiomyocyte stress induced by 4-hydroxynonenal

Bradford G. Hill, Brian P. Dranka, Luyun Zou, John C. Chatham, Victor M. Darley-Usmar
Journal: Biochemical Journal
Biochem J (2009) 424 (1): 99–107.
https://doi.org/10.1042/BJ20090934
Published: 23 October 2009
... or ischaemia [ 1 , 2 ]. However, this concept has proved technically difficult to examine using direct indices of mitochondrial function in intact myocytes. cardiomyocyte extracellular flux glycolysis heart lipid peroxidation mitochondrion oxidative stress Secondary products of oxidative stress...
View articletitled, Importance of the bioenergetic reserve capacity in response to cardiomyocyte stress induced by 4-hydroxynonenal
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Articles

Glycolysis in contracting rat skeletal muscle is controlled by factors related to energy state

Niels Ørtenblad, Will A. Macdonald, Kent Sahlin
Journal: Biochemical Journal
Biochem J (2009) 420 (2): 161–168.
https://doi.org/10.1042/BJ20082135
Published: 13 May 2009
...Niels Ørtenblad; Will A. Macdonald; Kent Sahlin The control of glycolysis in contracting muscle is not fully understood. The aim of the present study was to examine whether activation of glycolysis is mediated by factors related to the energy state or by a direct effect of Ca 2+ on the regulating...
View articletitled, Glycolysis in contracting rat skeletal muscle is controlled by factors related to energy state
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Articles

Inhibition of energy-producing pathways of HepG2 cells by 3-bromopyruvate 1

Ana Paula Pereira Da Silva, Tatiana El-Bacha, Nattascha Kyaw, Reinaldo Sousa Dos Santos, Wagner Seixas Da-Silva, Fabio C. L. Almeida, Andrea T. Da Poian, Antonio Galina
Journal: Biochemical Journal
Biochem J (2009) 417 (3): 717–726.
https://doi.org/10.1042/BJ20080805
Published: 16 January 2009
... cellular ATP production owing to its action on glycolysis and oxidative phosphorylation; however, the specific metabolic steps and mechanisms of 3-BrPA action in human hepatocellular carcinomas, particularly its effects on mitochondrial energetics, are poorly understood. In the present study it was found...
View articletitled, Inhibition of energy-producing pathways of HepG2 cells by 3-bromopyruvate 1
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Articles

Lactate favours the dissociation of skeletal muscle 6-phosphofructo-1-kinase tetramers down-regulating the enzyme and muscle glycolysis

Tiago Costa Leite, Daniel Da Silva, Raquel Guimarães Coelho, Patricia Zancan, Mauro Sola-Penna
Journal: Biochemical Journal
Biochem J (2007) 408 (1): 123–130.
https://doi.org/10.1042/BJ20070687
Published: 29 October 2007
...Tiago Costa Leite; Daniel Da Silva; Raquel Guimarães Coelho; Patricia Zancan; Mauro Sola-Penna For a long period lactate was considered as a dead-end product of glycolysis in many cells and its accumulation correlated with acidosis and cellular and tissue damage. At present, the role of lactate...
View articletitled, Lactate favours the dissociation of skeletal muscle 6-phosphofructo-1-kinase tetramers down-regulating the enzyme and muscle glycolysis
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Articles

Mapping of glycolytic enzyme-binding sites on human erythrocyte band 3

Haiyan Chu, Philip S. Low
Journal: Biochemical Journal
Biochem J (2006) 400 (1): 143–151.
https://doi.org/10.1042/BJ20060792
Published: 27 October 2006
... 5 2006 28 6 2006 12 7 2006 12 7 2006 The Biochemical Society, London 2006 band 3–peripheral protein interaction cytoplasmic domain of band 3 erythrocyte cytoskeleton erythrocyte membrane structure glycolysis glycolytic enzyme complex The GEs (glycolytic...
View articletitled, Mapping of glycolytic enzyme-binding sites on human erythrocyte band 3
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Articles

Simplified modelling of metabolic pathways for flux prediction and optimization: lessons from an in vitro reconstruction of the upper part of glycolysis

Julie B. Fiévet, Christine Dillmann, Gilles Curien, Dominique de vienne
Journal: Biochemical Journal
Biochem J (2006) 396 (2): 317–326.
https://doi.org/10.1042/BJ20051520
Published: 15 May 2006
... of glycolysis reconstituted in vitro , we showed how to derive, from titration experiments, values of effective enzyme activity parameters that do not include explicitly any of the classical kinetic constants. With a maximum of only two parameters per enzyme, this approach produced very good estimates...
View articletitled, Simplified modelling of metabolic pathways for flux prediction and optimization: lessons from an in vitro reconstruction of the upper part of glycolysis
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Articles

Expression of UCP3 in CHO cells does not cause uncoupling, but controls mitochondrial activity in the presence of glucose

Julien Mozo, Gilles Ferry, Aurélie Studeny, Claire Pecqueur, Marianne Rodriguez, Jean A. Boutin, Frédéric Bouillaud
Journal: Biochemical Journal
Biochem J (2006) 393 (1): 431–439.
https://doi.org/10.1042/BJ20050494
Published: 12 December 2005
... with the variable values observed with the 15 controls. Our observations suggest that, when glycolysis and mitochondria generate ATP, and in the absence of appropriate activators of proton transport, UCPs do not transport protons (uncoupling), but rather other ions of physiological relevance that control...
View articletitled, Expression of UCP3 in CHO cells does not cause uncoupling, but controls mitochondrial activity in the presence of glucose
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Articles

Triosephosphate isomerase deficiency: consequences of an inherited mutation at mRNA, protein and metabolic levels

Judit Oláh, Ferenc Orosz, László G. Puskás, László Hackler, Jr, Margit Horányi, László Polgár, Susan Hollán, Judit Ovádi
Journal: Biochemical Journal
Biochem J (2005) 392 (3): 675–683.
https://doi.org/10.1042/BJ20050993
Published: 06 December 2005
... mutations (F240L and E145Stop), but only the younger one suffers from neurodegeneration. In the present study, we determined the kinetic parameters of key glycolytic enzymes including the mutant TPI for rational modelling of erythrocyte glycolysis. We found that a low TPI activity in the mutant cells (lower...
View articletitled, Triosephosphate isomerase deficiency: consequences of an inherited mutation at mRNA, protein and metabolic levels
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Includes: Supplementary data
Articles

Inhibition of mitochondrial respiration by nitric oxide rapidly stimulates cytoprotective GLUT3-mediated glucose uptake through 5′-AMP-activated protein kinase

Pilar CIDAD, Angeles ALMEIDA, Juan P. BOLAÑOS
Journal: Biochemical Journal
Biochem J (2004) 384 (3): 629–636.
https://doi.org/10.1042/BJ20040886
Published: 07 December 2004
...Pilar CIDAD; Angeles ALMEIDA; Juan P. BOLAÑOS Recently, we have reported that the inhibition of mitochondrial respiration by nitric oxide (NO) leads to an up-regulation of glycolysis and affords cytoprotection against energy failure through the stimulation of AMPK (5′-AMP-activated protein kinase...
View articletitled, Inhibition of mitochondrial respiration by nitric oxide rapidly stimulates cytoprotective GLUT3-mediated glucose uptake through 5′-AMP-activated protein kinase
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Articles

6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis

Mark H. RIDER, Luc BERTRAND, Didier VERTOMMEN, Paul A. MICHELS, Guy G. ROUSSEAU, Louis HUE
Journal: Biochemical Journal
Biochem J (2004) 381 (3): 561–579.
https://doi.org/10.1042/BJ20040752
Published: 27 July 2004
...Mark H. RIDER; Luc BERTRAND; Didier VERTOMMEN; Paul A. MICHELS; Guy G. ROUSSEAU; Louis HUE Fru-2,6-P 2 (fructose 2,6-bisphosphate) is a signal molecule that controls glycolysis. Since its discovery more than 20 years ago, inroads have been made towards the understanding of the structure–function...
View articletitled, 6-Phosphofructo-2-kinase/fructose-2,6-bisphosphatase: head-to-head with a bifunctional enzyme that controls glycolysis
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Articles

Alteration of the bioenergetic phenotype of mitochondria is a hallmark of breast, gastric, lung and oesophageal cancer

Antonio ISIDORO, Marta MARTÍNEZ, Pedro L. FERNÁNDEZ, Álvaro D. ORTEGA, Gema SANTAMARÍA, Margarita CHAMORRO, John C. REED, José M. CUEZVA
Journal: Biochemical Journal
Biochem J (2004) 378 (1): 17–20.
https://doi.org/10.1042/bj20031541
Published: 15 February 2004
...: cancer, glycolysis, H+-ATP synthase, mitochondria. INTRODUCTION The H+-ATP synthase is the mitochondrial protein complex responsible for harnessing cellular ATP [1]. Efficient execution of programmed cell death also requires the activity and molecular components of the H+-ATP synthase [2 4]. Recently...
View articletitled, Alteration of the bioenergetic phenotype of mitochondria is a hallmark of breast, gastric, lung and oesophageal cancer
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Articles

Identification of C-terminal motifs responsible for transmission of inhibition by ATP of mammalian phosphofructokinase, and their contribution to other allosteric effects

Oscar H. MARTÍNEZ-COSTA, Carmen HERMIDA, Cristina SÁNCHEZ-MARTÍNEZ, Belén SANTAMARÍA, Juan J. ARAGÓN
Journal: Biochemical Journal
Biochem J (2004) 377 (1): 77–84.
https://doi.org/10.1042/bj20031032
Published: 01 January 2004
... correspondence should be addressed (e-mail [email protected] ). 9 7 2003 2 9 2003 16 9 2003 16 9 2003 The Biochemical Society, London ©2004 2004 allosteric regulation allosteric transition enzyme engineering enzyme regulation glycolysis phosphofructokinase...
View articletitled, Identification of C-terminal motifs responsible for transmission of inhibition by ATP of mammalian phosphofructokinase, and their contribution to other allosteric effects
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Articles

Metabolic analysis of senescent human fibroblasts reveals a role for AMP in cellular senescence

Werner ZWERSCHKE, Sybille MAZUREK, Petra STÖCKL, Eveline HÜTTER, Erich EIGENBRODT, Pidder JANSEN-DÜRR
Journal: Biochemical Journal
Biochem J (2003) 376 (2): 403–411.
https://doi.org/10.1042/bj20030816
Published: 01 December 2003
... premature senescence, reactive oxygen species are considered important intermediates contributing to the phenotype. Moreover, distinct alterations of the cellular carbohydrate metabolism are known to contribute to oncogenic transformation, as is best documented for the phenomenon of aerobic glycolysis...
View articletitled, Metabolic analysis of senescent human fibroblasts reveals a role for AMP in cellular senescence
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Articles

The unique features of glycolytic pathways in Archaea

Corné H. VERHEES, Servé W. M. KENGEN, Judith E. TUININGA, Gerrit J. SCHUT, Michael W. W. ADAMS, Willem M. de VOS, John van der OOST
Journal: Biochemical Journal
Biochem J (2003) 375 (2): 231–246.
https://doi.org/10.1042/bj20021472
Published: 15 October 2003
[email protected] ). 20 9 2002 16 5 2003 18 8 2003 18 8 2003 The Biochemical Society, London ©2003 2003 Archaea Embden—Meyerhof pathway Entner—Douderoff pathway glycolysis glycolytic enzyme polysaccharide Abbreviations used: AOR, aldehyde oxidoreductase; ED, Entner...
View articletitled, The unique features of glycolytic pathways in Archaea
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Articles

Reactive oxygen species mediate the down-regulation of mitochondrial transcripts and proteins by tumour necrosis factor-alpha in L929 cells

José A. SÁNCHEZ-ALCÁZAR, Erasmus SCHNEIDER, Inmaculada HERNÁNDEZ-MUÑOZ, Jesús RUIZ-CABELLO, Eva SILES-RIVAS, Paz de la TORRE, Belen BORNSTEIN, Gloria BREA, Joaquín ARENAS, Rafael GARESSE, José A. SOLÍS-HERRUZO, Alan J. KNOX, Plácido NAVAS
Journal: Biochemical Journal
Biochem J (2003) 370 (2): 609–619.
https://doi.org/10.1042/bj20021623
Published: 01 March 2003
... The Biochemical Society, London ©2003 2003 ATPase 6-8 free radical glycolysis mitochondrial transcript necrosis tumour necrosis factor α (TNF-α) Abbreviations used: BHA, butylated hydroxianisole; BHT, butylated hydroxytoluene; COX II, cytochrome c oxidase, subunit II; GAPDH, glyceraldehyde-3...
View articletitled, Reactive oxygen species mediate the down-regulation of mitochondrial transcripts and proteins by tumour necrosis factor-alpha in L929 cells
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Articles

An E2F-binding site mediates the activation of the proliferative isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by phosphatidylinositol 3-kinase

Silvia FERNÁNDEZ de MATTOS, Eric W.-F. LAM, Albert TAULER
Journal: Biochemical Journal
Biochem J (2002) 368 (1): 283–291.
https://doi.org/10.1042/bj20020622
Published: 15 November 2002
...-mail [email protected] ). 19 4 2002 16 7 2002 24 7 2002 24 7 2002 The Biochemical Society, London ©2002 2002 cell cycle E2F glycolysis Abbreviations used: BrdUrd, bromodeoxyuridine; cdk2, cyclin-dependent kinase 2; DCS, donor calf serum; DMEM, Dulbecco's...
View articletitled, An E2F-binding site mediates the activation of the proliferative isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase by phosphatidylinositol 3-kinase
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Articles

Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeast

Patrick Van DIJCK, José O. MASCORRO-GALLARDO, Martien De BUS, Katrien ROYACKERS, Gabriel ITURRIAGA, Johan M. THEVELEIN
Journal: Biochemical Journal
Biochem J (2002) 366 (1): 63–71.
https://doi.org/10.1042/bj20020517
Published: 15 August 2002
... 62210, Mexico. 4 To whom correspondence should be addressed (e-mail [email protected] ). 3 4 2002 23 4 2002 29 4 2002 The Biochemical Society, London ©2002 2002 glycolysis Saccharomyces cerevisiae sugar influx Abbreviations used...
View articletitled, Truncation of Arabidopsis thaliana and Selaginella lepidophylla trehalose-6-phosphate synthase unlocks high catalytic activity and supports high trehalose levels on expression in yeast
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Articles

Activation of glucokinase gene expression by hepatic nuclear factor 4α in primary hepatocytes

Ulrike ROTH, Kurt JUNGERMANN, Thomas KIETZMANN
Journal: Biochemical Journal
Biochem J (2002) 365 (1): 223–228.
https://doi.org/10.1042/bj20020340
Published: 01 July 2002
... 2002 12 4 2002 The Biochemical Society, London ©2002 2002 glycolysis hypoxia insulin maturity-onset diabetes of the young metabolic zonation nuclear receptor Abbreviations used: DIG, digoxigenin; EMSA, electrophoretic mobility-shift assay; EPO, erythropoietin; GK...
View articletitled, Activation of glucokinase gene expression by hepatic nuclear factor 4α in primary hepatocytes
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