... (10 nM) under serum-free conditions. binding CD109 glycoprotein inhibitor TGF-β isoforms trap Transforming growth factor-β (TGF-β) is a multifunctional growth factor involved in cell growth and differentiation, extracellular matrix deposition, cell adhesion and immunomodulation [ 1...

...Silvia Hüttner; Christiane Veit; Ulrike Vavra; Jennifer Schoberer; Martina Dicker; Daniel Maresch; Friedrich Altmann; Richard Strasser N -glycosylation of proteins plays an important role in the determination of the fate of newly synthesized glycoproteins in the endoplasmic reticulum (ER). Specific...

... in the endosome leads to the activation of their membrane fusion function. The HCV (hepatitis C virus) glycoprotein E1–E2 heterodimer mediates membrane fusion within the endosome, but the roles of conserved histidine residues in the formation of a functional heterodimer and in sensing pH changes is unknown. We...

... human SCUBE2 manifests as a secreted surface-anchored glycoprotein. Deletion mapping analysis defines the critical role of the spacer region and/or cysteine-rich repeats for membrane association. Further biochemical analyses and functional reporter assays demonstrated that human SCUBE2 can specifically...

.... Therefore, normal glycoprotein trafficking and processing through the glycosylation pathway is abrogated and glycoproteins are directed to undergo ERAD (ER-associated degradation), a consequence of which is the production of cytosolic FOS (free oligosaccharides). Following treatment with N B-DNJ, FOS were...

... the body by the serum glycoprotein, transferrin, and in mammals a related glycoprotein, lactoferrin, is produced to sequester iron on mucosal surfaces and at sites of infection. The presence of transferrin or lactoferrin in most ecological niches within the vertebrate or mammalian host restricts...

...Emmanuel Collec; Wassim El nemer; Emilie Gauthier; Pierre Gane; Marie-Christine Lecomte; Didier Dhermy; Jean Pierre Cartron; Yves Colin; Caroline Le van kim; Cécile Rahuel Lu (Lutheran) blood group and BCAM (basal cell adhesion molecule) antigens both reside on two gp (glycoprotein) isoforms, Lu...

... for Structural Bioinformatics) Protein Data Bank under the accession code 2AGJ. 27 10 2005 13 1 2006 20 1 2006 20 1 2006 The Biochemical Society, London 2006 antibody crystallography combinatorial peptide chemistry cryoglobulin glycoprotein IgM natural proteolytic...

..., muscle and kidney contained both isoforms; liver showed the highest expression, and the short form was predominant in this organ. In transiently transfected COS-1 cells, enzyme activity was markedly increased with gangliosides as well as with glycoproteins and oligosaccharides as substrates compared...

...Raffaella FRANCA; Emilija VELJKOVIC; Stefan WALTER; Carsten A. WAGNER; François VERREY The heteromeric amino acid transporter glycoprotein subunits rBAT and 4F2 hc (heavy chains) form, with different catalytic subunits (light chains), functional heterodimers that are covalently stabilized...

... (zona pellucida) glycoproteins. We have identified two glycoproteins, ZP1/gp97 and ZPC/gp42, as the major components of the chicken egg envelope. In the present study, another 42 kDa protein, designated ZPD, has been found as a new major component of the chicken egg envelope. ZPD was specifically...

... and characterized. Specific N-glycans are known to induce strong IgE responses of uncertain clinical relevance, but so far the allergenic potential of glycoproteins from hazelnut has not been investigated. The aim of the study was the molecular characterization of the glycosylated vicilin Cor a 11 from hazelnut...

... endogenous glycoprotein ligands via sugar-specific interactions, thus providing new insights into the occurrence and significance of the intravacuolar interaction shown by a legume lectin. 1 Present address: Department of Botany, Graduate School of Science, Kyoto University, Sakyo-ku, Kyoto 606-8502...

... line, MCF-7, showed a more complex pattern with both core 1- and core 2-based O-glycans. This is the first reported production of large quantities of recombinant MUC1 with a breast cancer-like O-glycosylation that could be used for the immunotherapy of breast cancer. Key words: glycoprotein...

...Andelko HRZENJAK; Sasa FRANK; Xingde WO; Yonggang ZHOU; Theo van BERKEL; Gert M. KOSTNER Lp(a) [lipoprotein (a)] is a highly atherogenic plasma lipoprotein assembled from low-density lipoprotein and the glycoprotein apolipoprotein (a). The rate of Lp(a) biosynthesis correlates significantly...

... used extensively to remove carbohydrate from glycoproteins, while leaving the protein backbone intact. Glycosylated proteins from animals, plants, fungi and bacteria have been deglycosylated with TFMS, and the most extensively studied types of carbohydrate chains in mammals, the N-linked, O-linked...

...Lami OYEWUMI; Feige KAPLAN; Neil B. SWEEZEY Secreted glycoproteins serve a variety of functions related to cell–cell communication in developmental systems. We cloned LGL1 , a novel glucocorticoid-inducible gene in foetal lung, and described its temporal and spatial localization in the rat...

...-specific glycoprotein (OGP), has been identified in oviductal fluid from various mammalian species, including humans. Although OGP is widely believed to be involved in the process of mammalian fertilization, including spermatozoon function and gamete interactions, based on experimental results obtained...

...-regulated protein 94), two chaperones whose binding is not carbohydrate-dependent. Our results suggest that calnexin and calreticulin, acting in ternary complexes with a large glycoprotein substrate such as Tg, might be engaged in the folding of distinct domains, and indicate that lumenal Ca 2+ strongly...

..., healthy domestic cats ( Felis catus ) excrete proteins at high concentrations (about 0.5mg/ml) in their urine. We investigated the possible cause of proteinuria in healthy cats, and discovered a 70kDa glycoprotein, which was excreted as a major urinary protein in cat urine, irrespective of gender...

...Shari A. PRICE-SCHIAVI; Aymee PEREZ; Roy BARCO; Kermit L. CARRAWAY Sialomucin complex (SMC/Muc4) is a heterodimeric glycoprotein complex consisting of a mucin subunit ascites sialoglycoprotein-1 (ASGP-1) and a transmembrane subunit (ASGP-2), which is aberrantly expressed on the surfaces...

... the cytosolic and nuclear fractions, although much more (approx. 3-fold) is found in the latter fraction. We propose that this bifunctional enzyme may be involved in both apoptotic DNA digestion and metabolism of cyclobutyl pyrimidine dimers in UV-irradiated human cells. Key words: apoptosis, glycoprotein...

...Yen-Hua HUANG; Sin-Tak CHU; Yee-Hsiung CHEN Seminal vesicle autoantigen (SVA) is a 19 kDa glycoprotein purified from mouse seminal vesicle secretion. It was quantified to be 0.9% (w/v) in the seminal vesicle fluid. We examined its distribution in the accessory sexual gland, characterized its...

... biosynthesis glycoprotein membrane protein processing topology Biochem. J. (1999) 339, 269 279 (Printed in Great Britain) 269 Transmembrane folding of the human erythrocyte anion exchanger (AE1, Band 3) determined by scanning and insertional N-glycosylation mutagenesis Milka POPOV, Jing LI and Reinhart...