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Elevated hypertrophy, growth plate maturation, glycosaminoglycan deposition, and exostosis formation in the Hspg2 exon 3 null mouse intervertebral disc

Cindy C. Shu, Susan M. Smith, Christopher B. Little, James Melrose

Biochem J (2019) 476 (2): 225–243.

https://doi.org/10.1042/BCJ20180695

Published: 18 January 2019

..., and thus, removal of perlecan HS/CS should affect extracellular matrix function and homeostasis. Hspg2 exon 3 null intervertebral discs accumulated significantly greater glycosaminoglycan in the nucleus pulposus, annulus fibrosus, and vertebral growth plates than C57BL/6 wild-type (WT) I intervertebral...

View articletitled, Elevated hypertrophy, growth plate maturation, glycosaminoglycan deposition, and exostosis formation in the Hspg2 exon 3 null mouse intervertebral disc

Articles

Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes

Satomi Nadanaka, Shoji Kagiyama, Hiroshi Kitagawa

Biochem J (2013) 454 (1): 133–145.

https://doi.org/10.1042/BJ20130323

Published: 26 July 2013

... to EXT1 and EXT2 , encodes an N-acetylhexosaminyltransferase. We have demonstrated that EXTL2 terminates chain elongation of GAGs (glycosaminoglycans), and thereby regulates GAG biosynthesis. The abnormal GAG biosynthesis caused by loss of EXTL2 had no effect on normal development or normal adult...

View articletitled, Roles of EXTL2, a member of the EXT family of tumour suppressors, in liver injury and regeneration processes

Includes: Supplementary data

Articles

Pentosan polysulfate increases affinity between ADAMTS-5 and TIMP-3 through formation of an electrostatically driven trimolecular complex

Linda Troeberg, Barbara Mulloy, Peter Ghosh, Meng-Huee Lee, Gillian Murphy, Hideaki Nagase

Biochem J (2012) 443 (1): 307–315.

https://doi.org/10.1042/BJ20112159

Published: 14 March 2012

... unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. adamalysin with thrombospondin motifs 5 (ADAMTS-5) aggrecanase extracellular matrix glycosaminoglycan osteoarthritis tissue inhibitor of metalloproteinases 3 (TIMP-3...

View articletitled, Pentosan polysulfate increases affinity between ADAMTS-5 and TIMP-3 through formation of an electrostatically driven trimolecular complex

Articles

Chondroitin 4- O -sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N -acetylgalactosaminyltransferase-1

Tomomi Izumikawa, Toshiyasu Koike, Hiroshi Kitagawa

Biochem J (2012) 441 (2): 697–705.

https://doi.org/10.1042/BJ20111472

Published: 21 December 2011

... be addressed (email [email protected] ). 11 8 2011 19 9 2011 27 9 2011 27 9 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 chondroitin sulfate glycosaminoglycan glycosyltransferase proteoglycan sulfotransferase CSs (chondroitin...

View articletitled, Chondroitin 4- O -sulfotransferase-2 regulates the number of chondroitin sulfate chains initiated by chondroitin N -acetylgalactosaminyltransferase-1

Articles

Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in co-operation with chondroitin N-acetylgalactosaminyltransferase-2

Tomomi Izumikawa, Yuka Okuura, Toshiyasu Koike, Naoki Sakoda, Hiroshi Kitagawa

Biochem J (2011) 434 (2): 321–331.

https://doi.org/10.1042/BJ20101456

Published: 11 February 2011

[email protected] ). 10 9 2010 29 11 2010 8 12 2010 8 12 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 chondroitin sulfate glycosaminoglycan glycosyltransferase proteoglycan sulfotransferase CS (chondroitin sulfate) is a class of GAG...

View articletitled, Chondroitin 4-O-sulfotransferase-1 regulates the chain length of chondroitin sulfate in co-operation with chondroitin N-acetylgalactosaminyltransferase-2

Includes: Supplementary data

Articles

Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development

Yumi Watanabe, Kosei Takeuchi, Susumu Higa Onaga, Michiko Sato, Mika Tsujita, Manabu Abe, Rie Natsume, Minqi Li, Tatsuya Furuichi, Mika Saeki, Tomomi Izumikawa, Ayumi Hasegawa, Minesuke Yokoyama, Shiro Ikegawa, Kenji Sakimura, Norio Amizuka, Hiroshi Kitagawa, Michihiro Igarashi

Biochem J (2010) 432 (1): 47–55.

https://doi.org/10.1042/BJ20100847

Published: 25 October 2010

... sulfate) is a glycosaminoglycan species that is widely distributed in the extracellular matrix. To understand the physiological roles of enzymes involved in CS synthesis, we produced CSGalNAcT1 (CS N-acetylgalactosaminyltransferase 1)-null mice. CS production was reduced by approximately half...

View articletitled, Chondroitin sulfate N-acetylgalactosaminyltransferase-1 is required for normal cartilage development

Articles

Biosynthesis of heparan sulfate in EXT1 -deficient cells

Megumi Okada, Satomi Nadanaka, Naoko Shoji, Jun-ichi Tamura, Hiroshi Kitagawa

Biochem J (2010) 428 (3): 463–471.

https://doi.org/10.1042/BJ20100101

Published: 27 May 2010

... 2 (EXT2) exostosin-like 2 (EXTL2) glycosaminoglycan heparan sulfate (HS) proteoglycan HS (heparan sulfate) proteoglycans are ubiquitously found at the cell surface and in the extracellular matrix, affecting a variety of biological processes, including specific signalling pathways [ 1 – 4...

View articletitled, Biosynthesis of heparan sulfate in EXT1 -deficient cells

Includes: Supplementary data

Articles

Chondroitin 4- O -sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish

Shuji Mizumoto, Tadahisa Mikami, Daiki Yasunaga, Naoki Kobayashi, Hajime Yamauchi, Ayumi Miyake, Nobuyuki Itoh, Hiroshi Kitagawa, Kazuyuki Sugahara

Biochem J (2009) 419 (2): 387–399.

https://doi.org/10.1042/BJ20081639

Published: 27 March 2009

... AB097217, AB122017 and AB089140 for zebrafish C4ST-1, C4ST-2 and D4ST-1 respectively. 11 8 2008 12 12 2008 6 1 2009 6 1 2009 © The Authors Journal compilation © 2009 Biochemical Society 2009 chondroitin sulfate embryogenesis glycosaminoglycan sulfation...

View articletitled, Chondroitin 4- O -sulfotransferase-1 is required for somitic muscle development and motor axon guidance in zebrafish

Articles

SOX9 transduction increases chondroitin sulfate synthesis in cultured human articular chondrocytes without altering glycosyltransferase and sulfotransferase transcription

Simon R. Tew, Peraphan Pothacharoen, Theoni Katopodi, Timothy E. Hardingham

Biochem J (2008) 414 (2): 231–236.

https://doi.org/10.1042/BJ20080262

Published: 12 August 2008

... weight of CS (chondroitin sulfate) and is secreted by chondrocytes to form the characteristic GAG (glycosaminoglycan)-rich ECM (extracellular matrix) of cartilage. SOX9 expression rapidly falls during monolayer culture of isolated chondrocytes and this turns off aggrecan and associated CS synthesis. We...

View articletitled, SOX9 transduction increases chondroitin sulfate synthesis in cultured human articular chondrocytes without altering glycosyltransferase and sulfotransferase transcription

Articles

Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor

Tomomi Izumikawa, Toru Uyama, Yuka Okuura, Kazuyuki Sugahara, Hiroshi Kitagawa

Biochem J (2007) 403 (3): 545–552.

https://doi.org/10.1042/BJ20061876

Published: 12 April 2007

... ]. chondroitin sulfate chondroitin polymerization glycosyltransferase glycosaminoglycan protein interaction proteoglycan CS (chondroitin sulfate) is a class of GAG (glycosaminoglycan) that is distributed on the surface of virtually all cells and in the extracellular matrix. CS is covalently linked...

View articletitled, Involvement of chondroitin sulfate synthase-3 (chondroitin synthase-2) in chondroitin polymerization through its interaction with chondroitin synthase-1 or chondroitin-polymerizing factor

Articles

Degradation of extracellular matrix and its components by hypobromous acid

Martin D. Rees, Tane N. McNiven, Michael J. Davies

Biochem J (2007) 401 (2): 587–596.

https://doi.org/10.1042/BJ20061236

Published: 21 December 2006

...-minoglycan components of extracellular matrix (proteoglycans and hyaluronan) may localize the production of HOBr to these materials. It is shown in the present paper that the reaction of HOBr with glycosaminoglycans (heparan sulfate, heparin, chondroitin sulfate and hyaluronan) generates polymer-derived N...

View articletitled, Degradation of extracellular matrix and its components by hypobromous acid

Articles

Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan

Stephan Reitinger, Gerhard Thomas Laschober, Christine Fehrer, Brigitte Greiderer, Günter Lepperdinger

Biochem J (2007) 401 (1): 79–85.

https://doi.org/10.1042/BJ20060598

Published: 11 December 2006

... glycosaminoglycan hyaluronan hyaluronidase sperm adhesion molecule 1 (SPAM1) A small lobe of ovary was surgically removed from an anaesthetized adult Xenopus laevis female. The ovarian tissue was rinsed in O-R2 buffer (82.5 mM NaCl, 2.5 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 1 mM Na 2 HPO 4 and 5 mM Hepes...

View articletitled, Mouse testicular hyaluronidase-like proteins SPAM1 and HYAL5 but not HYALP1 degrade hyaluronan

Articles

Primary attachment of murine leukaemia virus vector mediated by particle-associated heparan sulfate proteoglycan

Nina Kureishy, Daisy Faruque, Colin D. Porter

Biochem J (2006) 400 (3): 421–430.

https://doi.org/10.1042/BJ20060825

Published: 28 November 2006

... sought to determine the nature of this interaction. Following an initial screen of GAGs (glycosaminoglycans) for their ability to inhibit virus binding and transduction, we have shown that production of virus from cells in which GAG sulfation is inhibited, or treatment of virus with heparinase III...

View articletitled, Primary attachment of murine leukaemia virus vector mediated by particle-associated heparan sulfate proteoglycan

Articles

Serase-1B, a new splice variant of polyserase-1/TMPRSS9, activates urokinase-type plasminogen activator and the proteolytic activation is negatively regulated by glycosaminoglycans

Yuushi Okumura, Masaki Hayama, Etsuhisa Takahashi, Mieko Fujiuchi, Aki Shimabukuro, Mihiro Yano, Hiroshi Kido

Biochem J (2006) 400 (3): 551–561.

https://doi.org/10.1042/BJ20060212

Published: 28 November 2006

... inhibitors. Furthermore, this activation was also negatively regulated by glycosaminoglycans. Our results indicate that Serase-1B is a novel member of TTSPs that might be involved in uPA/plasmin-mediated proteolysis and possibly implicated in biological events such as fibrinolysis and tumour progression...

View articletitled, Serase-1B, a new splice variant of polyserase-1/TMPRSS9, activates urokinase-type plasminogen activator and the proteolytic activation is negatively regulated by glycosaminoglycans

Articles

Heparan sulfate 6- O -endosulfatases: discrete in vivo activities and functional co-operativity

William C. Lamanna, Rebecca J. Baldwin, Michael Padva, Ina Kalus, Gerdy ten Dam, Toin H. van Kuppevelt, John T. Gallagher, Kurt von Figura, Thomas Dierks, Catherine L. R. Merry

Biochem J (2006) 400 (1): 63–73.

https://doi.org/10.1042/BJ20060848

Published: 27 October 2006

... changes in HS structure suggest a novel method for the in vivo detection of aberrant mSulf activity. endosulfatase glycosaminoglycan heparan sulfate knock-out mice signal transduction sulfatase Dulbecco's modified Eagle's medium and foetal calf serum were from Life Technologies...

View articletitled, Heparan sulfate 6- O -endosulfatases: discrete in vivo activities and functional co-operativity

Includes: Supplementary data

Articles

In vivo contribution of amino acid sulfur to cartilage proteoglycan sulfation

Fabio Pecora, Benedetta Gualeni, Antonella Forlino, Andrea Superti-Furga, Ruggero Tenni, Giuseppe Cetta, Antonio Rossi

Biochem J (2006) 398 (3): 509–514.

https://doi.org/10.1042/BJ20060566

Published: 29 August 2006

... cartilage diastrophic dysplasia sulfate transporter glycosaminoglycan N -acetyl- L -cysteine proteoglycan Sulfation occurs in all tissues and involves proteins, lipids, hormones and drugs. The control and degree of sulfation of such a wide range of substances suggest that this pathway...

View articletitled, In vivo contribution of amino acid sulfur to cartilage proteoglycan sulfation

Articles

Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis

James Beattie, Gordon J. Allan, Jennifer D. Lochrie, David J. Flint

Biochem J (2006) 395 (1): 1–19.

https://doi.org/10.1042/BJ20060086

Published: 15 March 2006

... components in this region. extracellular matrix (ECM) glycosaminoglycan insulin-like growth factor-I (IGF-I) insulin-like growth factor-binding protein 5 (IGFBP-5) mammary gland proteolysis The IGF (insulin-like growth factor) axis plays a crucial role in regulating cellular growth...

View articletitled, Insulin-like growth factor-binding protein-5 (IGFBP-5): a critical member of the IGF axis

Articles

Human xylosyltransferase I and N-terminal truncated forms: functional characterization of the core enzyme

Sandra Müller, Jennifer Disse, Manuela Schöttler, Sylvia Schön, Christian Prante, Thomas Brinkmann, Joachim Kuhn, Knut Kleesiek, Christian Götting

Biochem J (2006) 394 (1): 163–171.

https://doi.org/10.1042/BJ20051606

Published: 27 January 2006

...Sandra Müller; Jennifer Disse; Manuela Schöttler; Sylvia Schön; Christian Prante; Thomas Brinkmann; Joachim Kuhn; Knut Kleesiek; Christian Götting Human XT-I (xylosyltransferase I; EC 2.4.2.26) initiates the biosynthesis of the glycosaminoglycan linkage region and is a diagnostic marker...

View articletitled, Human xylosyltransferase I and N-terminal truncated forms: functional characterization of the core enzyme

Articles

Oxidation of heparan sulphate by hypochlorite: role of N-chloro derivatives and dichloramine-dependent fragmentation

Martin D. Rees, David I. Pattison, Michael J. Davies

Biochem J (2005) 391 (1): 125–134.

https://doi.org/10.1042/BJ20050630

Published: 26 September 2005

... at pH 7.4. As MPO binds to glycosaminoglycans, oxidation of extracellular matrix and cell surfaces by HOCl may be localized to these materials. However, the reactions of HOCl with glycosaminoglycans are poorly characterized. The GlcNAc ( N -acetylglucosamine), GlcNSO 3 (glucosamine- N -sulphate...

View articletitled, Oxidation of heparan sulphate by hypochlorite: role of N-chloro derivatives and dichloramine-dependent fragmentation

Includes: Supplementary data

Articles

Fibroblast growth factors share binding sites in heparan sulphate

Johan KREUGER, Per JEMTH, Emil SANDERS-LINDBERG, Liat ELIAHU, Dina RON, Claudio BASILICO, Markku SALMIVIRTA, Ulf LINDAHL

Biochem J (2005) 389 (1): 145–150.

https://doi.org/10.1042/BJ20042129

Published: 21 June 2005

... targeting of different FGFs. 1 To whom correspondence should be addressed (email [email protected] ). 22 12 2004 23 2 2005 16 3 2005 16 3 2005 The Biochemical Society, London 2005 co-receptor epitope fibroblast growth factor glycosaminoglycan heparan...

View articletitled, Fibroblast growth factors share binding sites in heparan sulphate

Includes: Supplementary data

Articles

Treatment of the mouse model of mucopolysaccharidosis type IIIB with lentiviral-NAGLU vector

Paola Di NATALE, Carmela Di DOMENICO, Nadia GARGIULO, Sigismondo CASTALDO, Enrico GONZALEZ Y REYERO, Pratibha MITHBAOKAR, Mario De FELICE, Antonia FOLLENZI, Luigi NALDINI, Guglielmo R. D. VILLANI

Biochem J (2005) 388 (2): 639–646.

https://doi.org/10.1042/BJ20041702

Published: 24 May 2005

... to mutations in the gene encoding NAGLU (α- N -acetylglucosaminidase), one of the enzymes required for the degradation of the GAG (glycosaminoglycan) heparan sulphate. No therapy exists for affected patients. We have shown previously the efficacy of lentiviral-NAGLU-mediated gene transfer in correcting...

View articletitled, Treatment of the mouse model of mucopolysaccharidosis type IIIB with lentiviral-NAGLU vector

Articles

Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity

Sandra MÜLLER, Manuela SCHÖTTLER, Sylvia SCHÖN, Christian PRANTE, Thomas BRINKMANN, Joachim KUHN, Christian GÖTTING, Knut KLEESIEK

Biochem J (2005) 386 (2): 227–236.

https://doi.org/10.1042/BJ20041206

Published: 22 February 2005

...Sandra MÜLLER; Manuela SCHÖTTLER; Sylvia SCHÖN; Christian PRANTE; Thomas BRINKMANN; Joachim KUHN; Christian GÖTTING; Knut KLEESIEK XT-I (xylosyltransferase I) is the initial enzyme in the post-translational biosynthesis of glycosaminoglycan chains in proteoglycans. To gain insight...

View articletitled, Human xylosyltransferase I: functional and biochemical characterization of cysteine residues required for enzymic activity

Articles

Chondroitinase ABC I from Proteus vulgaris : cloning, recombinant expression and active site identification

Vikas PRABHAKAR, Ishan CAPILA, Carlos J. BOSQUES, Kevin POJASEK, Ram SASISEKHARAN

Biochem J (2005) 386 (1): 103–112.

https://doi.org/10.1042/BJ20041222

Published: 08 February 2005

...Vikas PRABHAKAR; Ishan CAPILA; Carlos J. BOSQUES; Kevin POJASEK; Ram SASISEKHARAN GalAGs (galactosaminoglycans) are one subset of the GAG (glycosaminoglycan) family of chemically heterogeneous polysaccharides that are involved in a wide range of biological processes. These complex biomacromolecules...

View articletitled, Chondroitinase ABC I from Proteus vulgaris : cloning, recombinant expression and active site identification

Articles

Hypochlorite and superoxide radicals can act synergistically to induce fragmentation of hyaluronan and chondroitin sulphates

Martin D. REES, Clare L. HAWKINS, Michael J. DAVIES

Biochem J (2004) 381 (1): 175–184.

https://doi.org/10.1042/BJ20040148

Published: 22 June 2004

... (the physiological mixture of hypochlorous acid and its anion present at pH 7.4). Exposure of glycosaminoglycans to a MPO–H 2 O 2 –Cl − system or reagent HOCl generates long-lived chloramides [R-NCl-C(O)-R′] derived from the glycosamine N -acetyl functions. Decomposition of these species by transition metal ions...

View articletitled, Hypochlorite and superoxide radicals can act synergistically to induce fragmentation of hyaluronan and chondroitin sulphates

Articles

Effects of [3H]glucosamine concentration on [3H]chondroitin sulphate formation by cultured chondrocytes

Paula J. MROZ, Jeremiah E. SILBERT

Biochem J (2003) 376 (2): 511–515.

https://doi.org/10.1042/bj20030982

Published: 01 December 2003

... concentrations. Even at concentrations of [ 3 H]GlcN that were greater than could be achieved with standard doses of oral GlcN, there was significant dilution of exogenous GlcN. Furthermore, an artificial acceptor for glycosaminoglycan synthesis in cell culture, 4-methylumbelliferyl β- d -xyloside, did...

View articletitled, Effects of [3H]glucosamine concentration on [3H]chondroitin sulphate formation by cultured chondrocytes

Articles

Fragmentation of extracellular matrix by hypochlorous acid

Alan A. WOODS, Michael J. DAVIES

Biochem J (2003) 376 (1): 219–227.

https://doi.org/10.1042/bj20030715

Published: 15 November 2003

... should be addressed (e-mail [email protected] ). 15 5 2003 3 7 2003 11 8 2003 11 8 2003 The Biochemical Society, London ©2003 2003 chloramine extracellular matrix glycosaminoglycan hypochlorite myeloperoxidase protein oxidation Abbreviations used: ECM...

View articletitled, Fragmentation of extracellular matrix by hypochlorous acid

Articles

Overexpression of UDP-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of K5 polysaccharide

Elisabet ROMAN, Ian ROBERTS, Kerstin LIDHOLT, Marion KUSCHE-GULLBERG

Biochem J (2003) 374 (3): 767–772.

https://doi.org/10.1042/bj20030365

Published: 15 September 2003

...Elisabet ROMAN; Ian ROBERTS; Kerstin LIDHOLT; Marion KUSCHE-GULLBERG The Escherichia coli K5 capsular polysaccharide (glycosaminoglycan) chains are composed of the repeated disaccharide structure: -GlcAβ1,4-GlcNAcα1,4-(where GlcA is glucuronic acid and GlcNAc is N -acetyl- d -glucosamine). The GlcA...

View articletitled, Overexpression of UDP-glucose dehydrogenase in Escherichia coli results in decreased biosynthesis of K5 polysaccharide

Articles

Substrate specificities of mouse heparan sulphate glucosaminyl 6-O-sulphotransferases

Emanuel SMEDS, Hiroko HABUCHI, Anh-Tri DO, Eva HJERTSON, Helena GRUNDBERG, Koji KIMATA, Ulf LINDAHL, Marion KUSCHE-GULLBERG

Biochem J (2003) 372 (2): 371–380.

https://doi.org/10.1042/bj20021666

Published: 01 June 2003

...Emanuel SMEDS; Hiroko HABUCHI; Anh-Tri DO; Eva HJERTSON; Helena GRUNDBERG; Koji KIMATA; Ulf LINDAHL; Marion KUSCHE-GULLBERG Glycosaminoglycan heparan sulphate interacts with a variety of proteins, such as growth factors, cytokines, enzymes and inhibitors and, thus, influences cellular functions...

View articletitled, Substrate specificities of mouse heparan sulphate glucosaminyl 6-O-sulphotransferases

Articles

Purification and identification of a binding protein for pancreatic secretory trypsin inhibitor: a novel role of the inhibitor as an anti-granzyme A

Satoshi TSUZUKI, Yoshimasa KOKADO, Shigeki SATOMI, Yoshie YAMASAKI, Hirofumi HIRAYASU, Toshihiko IWANAGA, Tohru FUSHIKI

Biochem J (2003) 372 (1): 227–233.

https://doi.org/10.1042/bj20021891

Published: 15 May 2003

... thiobenzyl ester (BLT), and the BLT hydrolysis was inhibited by PSTI. Sulphated glycosaminoglycans, such as fucoidan or heparin, showed almost no effect on the inhibition of rGzmA by PSTI, whereas they decreased the inhibition by antithrombin III. In the present paper, we propose a novel role of PSTI...

View articletitled, Purification and identification of a binding protein for pancreatic secretory trypsin inhibitor: a novel role of the inhibitor as an anti-granzyme A

Articles

Biosynthesis of heparan sulphate with diverse structures and functions: two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties

Hiroko HABUCHI, Goichiro MIYAKE, Ken NOGAMI, Asato KUROIWA, Yoichi MATSUDA, Marion KUSCHE-GULLBERG, Osami HABUCHI, Masayuki TANAKA, Koji KIMATA

Biochem J (2003) 371 (1): 131–142.

https://doi.org/10.1042/bj20021259

Published: 01 April 2003

...). 9 8 2002 28 11 2002 19 12 2002 19 12 2002 The Biochemical Society, London ©2003 2003 cDNA cloning GlcNSO 3 -6-O-sulphation glycosaminoglycan Abbreviations used: CDSNS-heparin, completely desulphated and N-resulphated heparin; CHO, Chinese-hamster ovary; ΔDi-NS...

View articletitled, Biosynthesis of heparan sulphate with diverse structures and functions: two alternatively spliced forms of human heparan sulphate 6-O-sulphotransferase-2 having different expression patterns and properties

Articles

Interaction of heparin with internally quenched fluorogenic peptides derived from heparin-binding consensus sequences, kallistatin and anti-thrombin III

Daniel C. PIMENTA, Iseli L. NANTES, Eduardo S. de SOUZA, Bernard Le BONNIEC, Amando S. ITO, Ivarne L.S. TERSARIOL, Vitor OLIVEIRA, Maria A. JULIANO, Luiz JULIANO

Biochem J (2002) 366 (2): 435–446.

https://doi.org/10.1042/bj20020023

Published: 01 September 2002

...-mail [email protected] ). 4 1 2002 2 5 2002 8 5 2002 8 5 2002 The Biochemical Society, London ©2002 2002 binding assay glycosaminoglycan kallikrein time-resolved fluorescence Abbreviations used: Abz, o -aminobenzoic acid; EDDnp, N -(2,4-dinitrophenyl)ethylenediamine...

View articletitled, Interaction of heparin with internally quenched fluorogenic peptides derived from heparin-binding consensus sequences, kallistatin and anti-thrombin III

Articles

Molecular characterization of chicken syndecan-2 proteoglycan

Ligong CHEN, John R. COUCHMAN, Jacqueline SMITH, Anne WOODS

Biochem J (2002) 366 (2): 481–490.

https://doi.org/10.1042/bj20020711

Published: 01 September 2002

.... 6 5 2002 29 5 2002 31 5 2002 31 5 2002 The Biochemical Society, London ©2002 2002 embryonic development glycosaminoglycan heparan sulphate Abbreviations used: C1 and C2 regions, conserved regions 1 and 2; cSNP, coding single nucleotide polymorphism; GAG...

View articletitled, Molecular characterization of chicken syndecan-2 proteoglycan

Articles

The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies

Frank DIETZ, Sebastian FRANKEN, Kenya YOSHIDA, Hideji NAKAMURA, Joachim KAPPLER, Volkmar GIESELMANN

Biochem J (2002) 366 (2): 491–500.

https://doi.org/10.1042/bj20011811

Published: 01 September 2002

... proteins, bHRP-4–green fluorescent protein and mHDGF–green fluorescent protein, in HEK-293 cells demonstrates nuclear localization of the proteins. bHRP-4 and mHDGF bind to the glycosaminoglycans heparin and heparan sulphate, but not to chondroitin sulphate. Affinity constants determined...

View articletitled, The family of hepatoma-derived growth factor proteins: characterization of a new member HRP-4 and classification of its subfamilies

Articles

Fibroblast growth factor-2 binds to small heparin-derived oligosaccharides and stimulates a sustained phosphorylation of p42/44 mitogen-activated protein kinase and proliferation of rat mammary fibroblasts

Maryse DELEHEDDE, Malcolm LYON, John T. GALLAGHER, Philip S. RUDLAND, David G. FERNIG

Biochem J (2002) 366 (1): 235–244.

https://doi.org/10.1042/bj20011718

Published: 15 August 2002

..., glycosaminoglycan-deficient cells, FGF-2 alone or in the presence of disaccharides did not stimulate DNA synthesis and it only elicited an early transient dual phosphorylation of p42/44 mitogen-activated protein kinase (MAPK). In the same cells FGF-2 in the presence of tetrasaccharides and longer oligosaccharides...

View articletitled, Fibroblast growth factor-2 binds to small heparin-derived oligosaccharides and stimulates a sustained phosphorylation of p42/44 mitogen-activated protein kinase and proliferation of rat mammary fibroblasts

Articles

UDP-glucose pyrophosphorylase: up-regulation in hypertrophic cartilage and role in hyaluronan synthesis

Cordula MAGEE, Maria NURMINSKAYA, Thomas F. LINSENMAYER

Biochem J (2001) 360 (3): 667–674.

https://doi.org/10.1042/bj3600667

Published: 10 December 2001

... gene, UDPG-PPase, as well as the HAS genes. 1 To whom correspondence should be addressed (e-mail [email protected] ). 25 6 2001 7 9 2001 18 10 2001 The Biochemical Society, London ©2001 2001 chondrocyte extracellular matrix glycosaminoglycan hyaluronan...

View articletitled, UDP-glucose pyrophosphorylase: up-regulation in hypertrophic cartilage and role in hyaluronan synthesis

Articles

Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo

John D. SANDY, Christie VERSCHAREN

Biochem J (2001) 358 (3): 615–626.

https://doi.org/10.1042/bj3580615

Published: 10 September 2001

... activity in vivo is destructive to cartilage matrix, since the bulk of the glycosaminoglycan (GAG)-bearing products are released from the tissue into the synovial fluid following cleavage of the Glu 373 –Ala 374 bond. In contrast, the MMP-like activity appears to be essentially non-destructive, since much...

View articletitled, Analysis of aggrecan in human knee cartilage and synovial fluid indicates that aggrecanase (ADAMTS) activity is responsible for the catabolic turnover and loss of whole aggrecan whereas other protease activity is required for C-terminal processing in vivo

Articles

Age-related changes in the sulphation of the chondroitin sulphate linkage region from human articular cartilage aggrecan

Robert M. LAUDER, Thomas N. HUCKERBY, Gavin M. BROWN, Michael T. BAYLISS, Ian A. NIEDUSZYNSKI

Biochem J (2001) 358 (2): 523–528.

https://doi.org/10.1042/bj3580523

Published: 24 August 2001

... The Biochemical Society, London ©2001 2001 ABC lyase chondroitin sulphate endolyase glycosaminoglycan osteoarthritis Abbreviations used: CS, chondroitin sulphate; GAG, glycosaminoglycan; Gal, galactose; GalNAc, N -acetylgalactosamine; GlcA, glucuronic acid; HPAEC, high-pH anion-exchange...

View articletitled, Age-related changes in the sulphation of the chondroitin sulphate linkage region from human articular cartilage aggrecan

Articles

Ca2+/calmodulin-dependent protein kinase mediates the phosphorylation of CD44 required for cell migration on hyaluronan

Charlotte A. LEWIS, Paul A. TOWNSEND, Clare M. ISACKE

Biochem J (2001) 357 (3): 843–850.

https://doi.org/10.1042/bj3570843

Published: 25 July 2001

...Charlotte A. LEWIS; Paul A. TOWNSEND; Clare M. ISACKE CD44 is the principal cell surface receptor for the extracellular matrix glycosaminoglycan hyaluronan, and binding to this ligand underlies CD44-mediated cell attachment and migration. As would be expected for a widely expressed adhesion...

View articletitled, Ca2+/calmodulin-dependent protein kinase mediates the phosphorylation of CD44 required for cell migration on hyaluronan

Articles

The nematode Caenorhabditis elegans synthesizes unusual O-linked glycans: identification of glucose-substituted mucin-type O-glycans and short chondroitin-like oligosaccharides

Yann GUÉRARDEL, Luis BALANZINO, Emmanuel MAES, Yves LEROY, Bernadette CODDEVILLE, Rafael ORIOL, Gérard STRECKER

Biochem J (2001) 357 (1): 167–182.

https://doi.org/10.1042/bj3570167

Published: 25 June 2001

... for glycosyltransferases involved in the synthesis of N- and O-linked glycans have already been isolated, but, apart from repetitive dimers of glycosaminoglycans, no detailed structure of either type of component has been published so far. This study aimed to establish the structures of the major O-glycans synthesized...

View articletitled, The nematode Caenorhabditis elegans synthesizes unusual O-linked glycans: identification of glucose-substituted mucin-type O-glycans and short chondroitin-like oligosaccharides

Articles

Differential regulation and expression of hyaluronan synthases in human articular chondrocytes, synovial cells and osteosarcoma cells

Anneliese D. RECKLIES, Chantal WHITE, Lee MELCHING, Peter J. ROUGHLEY

Biochem J (2001) 354 (1): 17–24.

https://doi.org/10.1042/bj3540017

Published: 08 February 2001

[email protected] ). 19 7 2000 1 11 2000 20 11 2000 The Biochemical Society, London © 2001 2001 cartilage cytokines extracellular matrix glycosaminoglycan Biochem. J. (2001) 354, 17 24 (Printed in Great Britain) 17 Differential regulation and expression of hyaluronan synthases...

View articletitled, Differential regulation and expression of hyaluronan synthases in human articular chondrocytes, synovial cells and osteosarcoma cells

Articles

Roles of aggrecan domains in biosynthesis, modification by glycosaminoglycans and product secretion

Chris KIANI, Vivian LEE, Liu CAO, Liwen CHEN, Yaojiong WU, Yaou ZHANG, Mark E. ADAMS, Burton B. YANG

Biochem J (2001) 354 (1): 199–207.

https://doi.org/10.1042/bj3540199

Published: 08 February 2001

... cloned and expressed these domains in various combinations in COS-7 cells. The results indicated that the G3 domain enhanced product secretion, alone or in combination with the KS or CS domain, and promoted glycosaminoglycan (GAG) chain attachment. Constructs containing the G1 domain were not secreted...

View articletitled, Roles of aggrecan domains in biosynthesis, modification by glycosaminoglycans and product secretion

Articles

Catabolism of aggrecan, decorin and biglycan in tendon

Sarah G. REES, Carl R. FLANNERY, Chris B. LITTLE, Clare E. HUGHES, Bruce CATERSON, Colin M. DENT

Biochem J (2000) 350 (1): 181–188.

https://doi.org/10.1042/bj3500181

Published: 09 August 2000

... and mature bovine tendons. A panel of well-characterized antibodies that recognize glycosaminoglycan or protein (linear or neoepitope) sequences was used to detect proteoglycans and proteoglycan degradation products that were both retained within the tissue and released into the culture medium. In addition...

View articletitled, Catabolism of aggrecan, decorin and biglycan in tendon

Articles

Purification and characterization of heparan sulphate proteoglycan from bovine brain

Youmie PARK, Guyong YU, Nur Sibel GUNAY, Robert J. LINHARDT

Biochem J (1999) 344 (3): 723–730.

https://doi.org/10.1042/bj3440723

Published: 08 December 1999

... and a core protein size of 66 kDa following the removal of glycosaminoglycan chains. Fractionation on DEAE-Sephacel showed that this proteoglycan consisted of three major forms having high, intermediate and low overall charge. All core proteins were identical in size and reacted with heparan sulphate...

View articletitled, Purification and characterization of heparan sulphate proteoglycan from bovine brain

Articles

Protective role for proteoglycans against cationic lipid cytotoxicity allowing optimal transfection efficiency in vitro

Mattias BELTING, Per PETERSSON

Biochem J (1999) 342 (2): 281–286.

https://doi.org/10.1042/bj3420281

Published: 24 August 1999

... cell lines. The growth-inhibitory effect of cationic lipids was abrogated by the addition of exogenous sulphated glycosaminoglycans. We conclude that the glycosaminoglycan part of proteoglycans serves a protective role against cationic lipid cytotoxicity, allowing optimal transfection efficiency...

View articletitled, Protective role for proteoglycans against cationic lipid cytotoxicity allowing optimal transfection efficiency in vitro

Articles

Involvement of the core protein in the first β-N-acetylgalactosamine transfer to the glycosaminoglycan–protein linkage-region tetrasaccharide and in the subsequent polymerization: the critical determining step for chondroitin sulphate biosynthesis

Satomi NADANAKA, Hiroshi KITAGAWA, Fumitaka GOTO, Jun-ichi TAMURA, Klaus W. NEUMANN, Tomoya OGAWA, Kazuyuki SUGAHARA

Biochem J (1999) 340 (2): 353–357.

https://doi.org/10.1042/bj3400353

Published: 25 May 1999

...Satomi NADANAKA; Hiroshi KITAGAWA; Fumitaka GOTO; Jun-ichi TAMURA; Klaus W. NEUMANN; Tomoya OGAWA; Kazuyuki SUGAHARA α-Thrombomodulin (α-TM) with a truncated glycosaminoglycan-protein linkage tetrasaccharide, GlcAβ1-3Galβ1-3Galβ1-4Xyl, was tested as an acceptor together with a sugar donor, UDP- N...

View articletitled, Involvement of the core protein in the first β-N-acetylgalactosamine transfer to the glycosaminoglycan–protein linkage-region tetrasaccharide and in the subsequent polymerization: the critical determining step for chondroitin sulphate biosynthesis

Articles

Sequence analysis of heparan sulphate and heparin oligosaccharides

Romain R. VIVÈS, David A. PYE, Markku SALMIVIRTA, John J. HOPWOOD, Ulf LINDAHL, John T. GALLAGHER

Biochem J (1999) 339 (3): 767–773.

https://doi.org/10.1042/bj3390767

Published: 26 April 1999

... of such structures is crucial for the complete understanding of glycosaminoglycan (GAG)-protein interactions. We describe here a simple method of sequence analysis relying on the specific tagging of the sugar reducing end by 3 H radiolabelling, the combination of chemical scission and specific enzymic digestion...

View articletitled, Sequence analysis of heparan sulphate and heparin oligosaccharides

Articles

Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation

Nicholas N. NISSEN, Ravi SHANKAR, Richard L. GAMELLI, Ashok SINGH, Luisa A. DiPIETRO

Biochem J (1999) 338 (3): 637–642.

https://doi.org/10.1042/bj3380637

Published: 08 March 1999

...). This study primarily focused on the capacity of non-enzymic glycosylation to influence intracellular bFGF. However, significant quantities of bFGF are also bound to the glycosaminoglycan (GAG), heparan sulphate, in the extracellular matrix (ECM) [6 8]. The capacity of non-enzymic glycosylation to alter...

View articletitled, Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation

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