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Articles

Common and unique features of glycosylation and glycosyltransferases in African trypanosomes

Samuel M. Duncan, Michael A.J. Ferguson

Biochem J (2022) 479 (17): 1743–1758.

https://doi.org/10.1042/BCJ20210778

Published: 06 September 2022

...Samuel M. Duncan; Michael A.J. Ferguson Eukaryotic protein glycosylation is mediated by glycosyl- and oligosaccharyl-transferases. Here, we describe how African trypanosomes exhibit both evolutionary conservation and significant divergence compared with other eukaryotes in how they synthesise...

View articletitled, Common and unique features of glycosylation and glycosyltransferases in African trypanosomes

Articles

Sialylation-dependent pharmacokinetics and differential complement pathway inhibition are hallmarks of CR1 activity in vivo

Biochem J (2022) 479 (9): 1007–1030.

https://doi.org/10.1042/BCJ20220054

Published: 13 May 2022

..., and more recently CSL040, a truncated version lacking the C-terminal long homologous repeat domain D (LHR-D). However, the role of N-linked glycosylation in determining its pharmacokinetic (PK) and pharmacodynamic (PD) properties is only partly understood. We demonstrated a relationship between the asialo...

View articletitled, Sialylation-dependent pharmacokinetics and differential complement pathway inhibition are hallmarks of CR1 activity in vivo

Includes: Supplementary data

Articles

Identification of a glycan cluster in gp120 essential for irreversible HIV-1 lytic inactivation by a lectin-based recombinantly engineered protein conjugate

Bibek Parajuli, Kriti Acharya, Aakansha Nangarlia, Shiyu Zhang, Bijay Parajuli, Alexej Dick, Brendon Ngo, Cameron F. Abrams, Irwin Chaiken

Biochem J (2020) 477 (21): 4263–4280.

https://doi.org/10.1042/BCJ20200495

Published: 13 November 2020

... if the reported four glycosylation sites are conserved in YU2 as well ( Figure 1 and Supplementary material S1 ). Since YU2 is a laboratory-adapted strain more suited for biophysical and virological assessment, this was important for comparative assessment. Sequence analysis showed that three of the four...

View articletitled, Identification of a glycan cluster in gp120 essential for irreversible HIV-1 lytic inactivation by a lectin-based recombinantly engineered protein conjugate

Includes: Supplementary data

Articles

The sugar code: letters and vocabulary, writers, editors and readers and biosignificance of functional glycan–lectin pairing

In Collection

Editors' pick of review article highlights published in the Biochemical Journal in 2018 & 2019

Herbert Kaltner, José Abad-Rodríguez, Anthony P. Corfield, Jürgen Kopitz, Hans-Joachim Gabius

Biochem J (2019) 476 (18): 2623–2655.

https://doi.org/10.1042/BCJ20170853

Published: 24 September 2019

... of the Biochemical Society 2019 adhesion gangliosides glycolipid glycoproteins glycosylation lectin Clearly, molecular languages of life govern cellular communication. The long and winding road that the concept of information coding by glycans has taken to gain acceptance is first surveyed...

View articletitled, The sugar code: letters and vocabulary, writers, editors and readers and biosignificance of functional glycan–lectin pairing

Includes: Supplementary data

Articles

The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated

Inderjeet Kaur, Salla Ruskamo, Jarkko Koivunen, Ritva Heljasvaara, Jarkko J. Lackman, Valerio Izzi, Ulla E. Petäjä-Repo, Petri Kursula, Taina Pihlajaniemi

Biochem J (2018) 475 (22): 3577–3593.

https://doi.org/10.1042/BCJ20180405

Published: 20 November 2018

... kDa. Circular dichroism spectroscopy confirmed the disordered character of DUF959, and static light scattering indicated a monomeric state for glycosylated DUF959 in solution. Small-angle X-ray scattering showed DUF959 to be a highly extended, flexible molecule with a maximum dimension of ∼23 nm...

View articletitled, The N-terminal domain of unknown function (DUF959) in collagen XVIII is intrinsically disordered and highly O-glycosylated

Articles

Role of glycosylation in nucleating protein folding and stability

Nisha Grandhi Jayaprakash, Avadhesha Surolia

Biochem J (2017) 474 (14): 2333–2347.

https://doi.org/10.1042/BCJ20170111

Published: 03 July 2017

...Nisha Grandhi Jayaprakash; Avadhesha Surolia Glycosylation constitutes one of the most common, ubiquitous and complex forms of post-translational modification. It commences with the synthesis of the protein and plays a significant role in deciding its folded state, oligomerization and thus its...

View articletitled, Role of glycosylation in nucleating protein folding and stability

Articles

Manganese-induced turnover of TMEM165

Sven Potelle, Eudoxie Dulary, Leslie Climer, Sandrine Duvet, Willy Morelle, Dorothée Vicogne, Elodie Lebredonchel, Marine Houdou, Corentin Spriet, Marie-Ange Krzewinski-Recchi, Romain Peanne, André Klein, Geoffroy de Bettignies, Pierre Morsomme, Gert Matthijs, Thorsten Marquardt, Vladimir Lupashin, François Foulquier

Biochem J (2017) 474 (9): 1481–1493.

https://doi.org/10.1042/BCJ20160910

Published: 19 April 2017

...; François Foulquier TMEM165 deficiencies lead to one of the congenital disorders of glycosylation (CDG), a group of inherited diseases where the glycosylation process is altered. We recently demonstrated that the Golgi glycosylation defect due to TMEM165 deficiency resulted from a Golgi manganese...

View articletitled, Manganese-induced turnover of TMEM165

Includes: Supplementary data

Articles

N-glycosylation of human sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is essential for stability, secretion and activity

Mathew Traini, Raani Kumaran, Morten Thaysen-Andersen, Maaike Kockx, Wendy Jessup, Leonard Kritharides

Biochem J (2017) 474 (7): 1071–1092.

https://doi.org/10.1042/BCJ20160735

Published: 08 March 2017

... function in vivo , remain obscure. Here, we investigate the roles of N-glycosylation in the expression, secretion and activity of human SMPDL3A, using inhibitors of N-glycosylation and site-directed mutagenesis, with either THP-1 macrophages or CHO cells expressing human SMPDL3A. Tunicamycin (TM) treatment...

View articletitled, N-glycosylation of human sphingomyelin phosphodiesterase acid-like 3A (SMPDL3A) is essential for stability, secretion and activity

Includes: Supplementary data

Articles

Giant mimivirus R707 encodes a glycogenin paralogue polymerizing glucose through α- and β-glycosidic linkages

Anna J. Rommel, Andreas J. Hülsmeier, Simon Jurt, Thierry Hennet

Biochem J (2016) 473 (20): 3451–3462.

https://doi.org/10.1042/BCJ20160280

Published: 11 October 2016

... of a glycosyltransferase capable of forming α and β linkages underlines the peculiarity of mimivirus and enforces the concept of a host-independent glycosylation machinery in mimivirus. Correspondence: Thierry Hennet ( [email protected] ) 1 4 2016 4 7 2016 18 7 2016 18 7 2016 © 2016...

View articletitled, Giant mimivirus R707 encodes a glycogenin paralogue polymerizing glucose through α- and β-glycosidic linkages

Includes: Supplementary data

Articles

Physical and functional interactions between the serotonin transporter and the neutral amino acid transporter ASCT2

Pascal Seyer, Franck Vandermoere, Elisabeth Cassier, Joël Bockaert, Philippe Marin

Biochem J (2016) 473 (13): 1953–1965.

https://doi.org/10.1042/BCJ20160315

Published: 28 June 2016

... in serotonergic neurons and involved in the transport of small neutral amino acids across the plasma membrane. Co-expression of ASCT2 with SERT in HEK (human embryonic kidney)-293 cells affects glycosylation and cell-surface localization of SERT with a concomitant reduction in its 5-HT uptake activity. Conversely...

View articletitled, Physical and functional interactions between the serotonin transporter and the neutral amino acid transporter ASCT2

Includes: Supplementary data

Articles

Glycosylation of matrix metalloproteases and tissue inhibitors: present state, challenges and opportunities

Lise Boon, Estefania Ugarte-Berzal, Jennifer Vandooren, Ghislain Opdenakker

Biochem J (2016) 473 (11): 1471–1482.

https://doi.org/10.1042/BJ20151154

Published: 27 May 2016

... of signalling events, zymogen activation, post-translational modifications and extracellular inhibition. Slight imbalances may result in the initiation or progression of specific disease states, such as cancer and pathological inflammation. As glycosylation modifies the structures and functions of glycoproteins...

View articletitled, Glycosylation of matrix metalloproteases and tissue inhibitors: present state, challenges and opportunities

Includes: Supplementary data

Articles

Use of a mutant OGA for detecting O-GlcNAc modified proteins

Robert J. Chalkley

Biochem J (2015) 472 (2): e25–e26.

https://doi.org/10.1042/BJ20150970

Published: 13 November 2015

... of Mariappa et al. advances the field. glycosylation mass spectrometry OGA O-linked N -acetylglucosamine (O-GlcNAc) Cells employ a large selection of post-translational modifications (PTMs) to dynamically alter the activity of proteins. One of the most common is the addition of a single N...

View articletitled, Use of a mutant OGA for detecting O-GlcNAc modified proteins

Articles

The dimeric crystal structure of the human fertility lipocalin glycodelin reveals a protein scaffold for the presentation of complex glycans

André Schiefner, Fabian Rodewald, Irmgard Neumaier, Arne Skerra

Biochem J (2015) 466 (1): 95–104.

https://doi.org/10.1042/BJ20141003

Published: 06 February 2015

... and S–that arise from different N-linked oligosaccharide side chains at amino acid residues Asn28 and Asn63. We have expressed Gd (carrying two amino acid substitutions to improve solubility) as a non-glycosylated protein in Escherichia coli via periplasmic secretion and determined its X-ray structure...

View articletitled, The dimeric crystal structure of the human fertility lipocalin glycodelin reveals a protein scaffold for the presentation of complex glycans

Articles

A context-independent N -glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation

Silvia Hüttner, Christiane Veit, Ulrike Vavra, Jennifer Schoberer, Martina Dicker, Daniel Maresch, Friedrich Altmann, Richard Strasser

Biochem J (2014) 464 (3): 401–411.

https://doi.org/10.1042/BJ20141057

Published: 05 December 2014

...Silvia Hüttner; Christiane Veit; Ulrike Vavra; Jennifer Schoberer; Martina Dicker; Daniel Maresch; Friedrich Altmann; Richard Strasser N -glycosylation of proteins plays an important role in the determination of the fate of newly synthesized glycoproteins in the endoplasmic reticulum (ER). Specific...

View articletitled, A context-independent N -glycan signal targets the misfolded extracellular domain of Arabidopsis STRUBBELIG to endoplasmic-reticulum-associated degradation

Articles

Glycosylation at Asn 91 of H1N1 haemagglutinin affects binding to glycan receptors

Akila Jayaraman, Xiaoying Koh, Jing Li, Rahul Raman, Karthik Viswanathan, Zachary Shriver, Ram Sasisekharan

Biochem J (2012) 444 (3): 429–435.

https://doi.org/10.1042/BJ20112101

Published: 29 May 2012

... membrane to the host nuclear membrane during viral replication. Given the abundance of HA on the viral surface, this protein is also the primary target for host innate and adaptive immune responses. Although addition of glycosylation sites on HA are a part of viral evolution to evade the host immune...

View articletitled, Glycosylation at Asn 91 of H1N1 haemagglutinin affects binding to glycan receptors

Includes: Supplementary data

Articles

Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

Heike Riegler, Thomas Herter, Irina Grishkovskaya, Anja Lude, Malgorzata Ryngajllo, Marie E. Bolger, Bernd Essigmann, Björn Usadel

Biochem J (2012) 443 (2): 427–437.

https://doi.org/10.1042/BJ20112071

Published: 27 March 2012

... are above or to the right of a reaction arrow. Prokaryotic enzymes are indicated below or left of a reaction arrow. Arabidopsis thaliana glucosamine-6-phosphate N-acetyltransferase (GNA) glycosylation N -acetylglucosamine biosynthesis nucleotide sugar GlcNAc ( N -acetylglucosamine...

View articletitled, Crystal structure and functional characterization of a glucosamine-6-phosphate N-acetyltransferase from Arabidopsis thaliana

Includes: Supplementary data

Articles

A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability

Peng Huang, Chongguang Chen, Stephen D. Mague, Julie A. Blendy, Lee-Yuan Liu-Chen

Biochem J (2012) 441 (1): 379–386.

https://doi.org/10.1042/BJ20111050

Published: 14 December 2011

... the equivalent nucleotide/amino acid substitution (A112G; N38D) of the A118G SNP in the hMOPR gene, we investigated the N-linked glycosylation status of thalamic and striatal MOPR in G/G mice compared with A/A mice (wild-type mice homozygous for the 112A allele of MOPR). The molecular mass of MOPR determined...

View articletitled, A common single nucleotide polymorphism A118G of the μ opioid receptor alters its N-glycosylation and protein stability

Articles

Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Hwajin Jung, Suk Kyung Lee, Eek-hoon Jho

Biochem J (2011) 436 (2): 263–269.

https://doi.org/10.1042/BJ20101512

Published: 13 May 2011

... inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic...

View articletitled, Mest/Peg1 inhibits Wnt signalling through regulation of LRP6 glycosylation

Articles

Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130 Cas

Caroline Pellet-Many, Paul Frankel, Ian M. Evans, Birger Herzog, Manfred Jünemann-Ramírez, Ian C. Zachary

Biochem J (2011) 435 (3): 609–618.

https://doi.org/10.1042/BJ20100580

Published: 13 April 2011

... growth factor), and to identify the signalling mechanisms involved. NRP1 is highly expressed in HAoSMCs (human aortic smooth muscle cells) and HCASMCs, and modified in VSMCs by CS (chondroitin sulfate)-rich O-linked glycosylation at Ser 612 . HCASMC migration induced by PDGF-BB and PDGF-AA was inhibited...

View articletitled, Neuropilin-1 mediates PDGF stimulation of vascular smooth muscle cell migration and signalling via p130 Cas

Includes: Supplementary data

Articles

Regulation of pendrin by pH: dependence on glycosylation

Anie Azroyan, Kamel Laghmani, Gilles Crambert, David Mordasini, Alain Doucet, Aurélie Edwards

Biochem J (2011) 434 (1): 61–72.

https://doi.org/10.1042/BJ20101411

Published: 27 January 2011

... with intracellular pH, with increased activity at low intracellular pH. Maximal pendrin activity was also stimulated at low extracellular pH, suggesting the presence of both intracellular and extracellular proton regulatory sites. We identified five putative pendrin glycosylation sites, only two of which are used...

View articletitled, Regulation of pendrin by pH: dependence on glycosylation

Articles

The glycosylation of human synovial lubricin: implications for its role in inflammation

Ruby P. Estrella, John M. Whitelock, Nicolle H. Packer, Niclas G. Karlsson

Biochem J (2010) 429 (2): 359–367.

https://doi.org/10.1042/BJ20100360

Published: 28 June 2010

... corresponding to the sequence NeuAcα2–3Galβ1–3(NeuAcα2–3Galβ1–3/4GlcNAcβ1–6)GalNAc. It was also found that a small proportion of the core 2 oligosaccharides carried sulfate. The ability of lubricin to present complex glycosylation reflecting the state of the joint tissue makes lubricin a candidate as a carrier...

View articletitled, The glycosylation of human synovial lubricin: implications for its role in inflammation

Includes: Supplementary data

Articles

The presence of monoglucosylated N196-glycan is important for the structural stability of storage protein, arylphorin

Kyoung-Seok Ryu, Jie-Oh Lee, Taek Hun Kwon, Han-Ho Choi, Hong-Seog Park, Soo Kyung Hwang, Zee-Won Lee, Kyung-Bok Lee, Young Hyun Han, Yun-Seok Choi, Young Ho Jeon, Chaejoon Cheong, Soohyun Kim

Biochem J (2009) 421 (1): 87–96.

https://doi.org/10.1042/BJ20082170

Published: 12 June 2009

...Kyoung-Seok Ryu; Jie-Oh Lee; Taek Hun Kwon; Han-Ho Choi; Hong-Seog Park; Soo Kyung Hwang; Zee-Won Lee; Kyung-Bok Lee; Young Hyun Han; Yun-Seok Choi; Young Ho Jeon; Chaejoon Cheong; Soohyun Kim Although N-glycosylation has been known to increase the stability of glycoproteins, it is difficult...

View articletitled, The presence of monoglucosylated N196-glycan is important for the structural stability of storage protein, arylphorin

Includes: Supplementary data

Articles

The Nrf1 CNC/bZIP protein is a nuclear envelope-bound transcription factor that is activated by t-butyl hydroquinone but not by endoplasmic reticulum stressors

Yiguo Zhang, John M. Lucocq, John D. Hayes

Biochem J (2009) 418 (2): 293–310.

https://doi.org/10.1042/BJ20081575

Published: 11 February 2009

...Yiguo Zhang; John M. Lucocq; John D. Hayes In rat liver RL-34 cells, endogenous Nrf1 (nuclear factor-erythroid 2 p45 subunit-related factor 1) is localized in the ER (endoplasmic reticulum) where it exists as a glycosylated protein. Electron microscopy has demonstrated that ectopic Nrf1 in COS-1...

View articletitled, The Nrf1 CNC/bZIP protein is a nuclear envelope-bound transcription factor that is activated by t-butyl hydroquinone but not by endoplasmic reticulum stressors

Includes: Supplementary data

Articles

A novel role of the C-terminus of b 0,+ AT in the ER–Golgi trafficking of the rBAT–b 0,+ AT heterodimeric amino acid transporter

Shinichi Sakamoto, Arthit Chairoungdua, Shushi Nagamori, Pattama Wiriyasermkul, Kanyarat Promchan, Hidekazu Tanaka, Toru Kimura, Takeshi Ueda, Masaaki Fujimura, Yasuhiro Shigeta, Yukio Naya, Koichiro Akakura, Haruo Ito, Hitoshi Endou, Tomohiko Ichikawa, Yoshikatsu Kanai

Biochem J (2009) 417 (2): 441–448.

https://doi.org/10.1042/BJ20081798

Published: 23 December 2008

... composed of rBAT (related to b 0,+ amino acid transporter), a single-membrane-spanning glycosylated heavy chain, and b 0,+ AT, a putative 12-membrane-spanning non-glycosylated light chain, is an amino acid transporter that mediates the activity of system b 0,+ , a major apical transport system for cystine...

View articletitled, A novel role of the C-terminus of b 0,+ AT in the ER–Golgi trafficking of the rBAT–b 0,+ AT heterodimeric amino acid transporter

Includes: Supplementary data

Articles

The crystal structure of rabbit IgG-Fc

Enrico Girardi, Mary D. Holdom, Anna M. Davies, Brian J. Sutton, Andrew J. Beavil

Biochem J (2009) 417 (1): 77–83.

https://doi.org/10.1042/BJ20081355

Published: 12 December 2008

... fragment crystallizable (Fc) glycosylation immunoglobulin G (IgG) rabbit ( Oryctolagus cuniculus ) The rabbit ( Oryctolagus cuniculus ) immunoglobulin gamma, IgG, was one of the first molecules of the immune system to be investigated, and the pioneering studies of Porter [ 1 ] produced the very...

View articletitled, The crystal structure of rabbit IgG-Fc

Articles

Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective α 1b -adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers

Meritxell Canals, Juan F. Lopez-Gimenez, Graeme Milligan

Biochem J (2009) 417 (1): 161–172.

https://doi.org/10.1042/BJ20081227

Published: 12 December 2008

... -adrenoceptor antagonists that acted at the level of the endoplasmic reticulum. This was accompanied by maturation of the mutant receptor to a terminally N-glycosylated form, and only this mature form was trafficked to the cell surface. Co-expression of the mutant receptor with an otherwise wild-type form...

View articletitled, Cell surface delivery and structural re-organization by pharmacological chaperones of an oligomerization-defective α 1b -adrenoceptor mutant demonstrates membrane targeting of GPCR oligomers

Articles

Wnt signalling at the crossroads of nutritional regulation

Jaswinder K. Sethi, Antonio J. Vidal-Puig

Biochem J (2008) 416 (2): e11–e13.

https://doi.org/10.1042/BJ20082074

Published: 12 November 2008

... promoting N-linked glycosylation of Wnt-related proteins. The HBP is responsible for shuttling glucose to cellular glycosylation events. At the biochemical level, the enzyme GFAT (glutamine:fructose-6-phosphate amidotransferase) catalyses the conversion of fructose 6-phosphate (from glycolysis...

View articletitled, Wnt signalling at the crossroads of nutritional regulation

Articles

Assembly of a membrane receptor complex: roles of the uroplakin II prosequence in regulating uroplakin bacterial receptor oligomerization

Chih-Chi Andrew Hu, Thomas Bachmann, Ge Zhou, Feng-Xia Liang, Jorge Ghiso, Gert Kreibich, Tung-Tien Sun

Biochem J (2008) 414 (2): 195–203.

https://doi.org/10.1042/BJ20080550

Published: 12 August 2008

... formation in the prosequence domain of proUPII (the immature form of UPII still containing its prosequence); (ii) differentiation-dependent N-glycosylation of the prosequence leads to UP stabilization; (iii) a failure to form tetramers in cultured urothelial cells, in part due to altered glycosylation...

View articletitled, Assembly of a membrane receptor complex: roles of the uroplakin II prosequence in regulating uroplakin bacterial receptor oligomerization

Articles

IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa

Sophie Groux-Degroote, Marie-Ange Krzewinski-Recchi, Aurélie Cazet, Audrey Vincent, Sylvain Lehoux, Jean-Jacques Lafitte, Isabelle van Seuningen, Philippe Delannoy

Biochem J (2008) 410 (1): 213–223.

https://doi.org/10.1042/BJ20070958

Published: 29 January 2008

...Sophie Groux-Degroote; Marie-Ange Krzewinski-Recchi; Aurélie Cazet; Audrey Vincent; Sylvain Lehoux; Jean-Jacques Lafitte; Isabelle van Seuningen; Philippe Delannoy Bronchial mucins from patients suffering from CF (cystic fibrosis) exhibit glycosylation alterations, especially increased amounts...

View articletitled, IL-6 and IL-8 increase the expression of glycosyltransferases and sulfotransferases involved in the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa

Includes: Supplementary data

Articles

The NHB1 (N-terminal homology box 1) sequence in transcription factor Nrf1 is required to anchor it to the endoplasmic reticulum and also to enable its asparagine-glycosylation

Yiguo Zhang, John M. Lucocq, Masayuki Yamamoto, John D. Hayes

Biochem J (2007) 408 (2): 161–172.

https://doi.org/10.1042/BJ20070761

Published: 14 November 2007

... homology box 1; residues 11–30), which tethers Nrf1 to the ER (endoplasmic reticulum). Electrophoresis resolved Nrf1 into two major bands of approx. 95 and 120 kDa. The 120-kDa Nrf1 form represents a glycosylated protein that was present exclusively in the ER and was converted into a substantially smaller...

View articletitled, The NHB1 (N-terminal homology box 1) sequence in transcription factor Nrf1 is required to anchor it to the endoplasmic reticulum and also to enable its asparagine-glycosylation

Includes: Supplementary data

Articles

Identification of a novel 82 kDa proMMP-9 species associated with the surface of leukaemic cells: (auto-)catalytic activation and resistance to inhibition by TIMP-1

Christian Ries, Thomas Pitsch, Reinhard Mentele, Stefan Zahler, Virginia Egea, Hideaki Nagase, Marianne Jochum

Biochem J (2007) 405 (3): 547–558.

https://doi.org/10.1042/BJ20070191

Published: 13 July 2007

...) were used. The developed films were scanned and analysed using the ImageMaster-1D Elite software. acute myeloid leukaemia gelatinase B glycosylation matrix metalloproteinase (MMP) tissue inhibitor of metalloproteinase (TIMP) tumour cell invasion MMPs (matrix metalloproteinases...

View articletitled, Identification of a novel 82 kDa proMMP-9 species associated with the surface of leukaemic cells: (auto-)catalytic activation and resistance to inhibition by TIMP-1

Articles

Critical roles for p22 phox in the structural maturation and subcellular targeting of Nox3

Yoko Nakano, Botond Banfi, Algirdas J. Jesaitis, Mary C. Dinauer, Lee-Ann H. Allen, William M. Nauseef

Biochem J (2007) 403 (1): 97–108.

https://doi.org/10.1042/BJ20060819

Published: 13 March 2007

..., the electron transferase of the phagocyte Nox. In vitro Nox3 cDNA expressed an ∼50 kDa primary translation product that underwent N-linked glycosylation in the presence of canine microsomes. RNAi (RNA interference)-mediated reduction of endogenous p22 phox , a subunit essential for stabilization of Nox2...

View articletitled, Critical roles for p22 phox in the structural maturation and subcellular targeting of Nox3

Includes: Supplementary data

Articles

Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling

Manabu Kurayoshi, Hideki Yamamoto, Shunsuke Izumi, Akira Kikuchi

Biochem J (2007) 402 (3): 515–523.

https://doi.org/10.1042/BJ20061476

Published: 26 February 2007

... palmitoylation of Wnt-5a. These results indicate that palmitoylation of Wnt-5a is important for the triggering of signalling at the cell surface level and, therefore, that the lipid-unmodified form of Wnt-5a cannot activate intracellular signal cascades. In contrast, glycosylation was necessary for the secretion...

View articletitled, Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling

Articles

MEGF9: a novel transmembrane protein with a strong and developmentally regulated expression in the nervous system

Ulrike Brandt-Bohne, Douglas R. Keene, Fletcher A. White, Manuel Koch

Biochem J (2007) 401 (2): 447–457.

https://doi.org/10.1042/BJ20060691

Published: 21 December 2006

... nervous system). The domain structure of MEGF9 consists of an N-terminal region with several potential O-glycosylation sites followed by five EGF-like domains, which are highly homologous with the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short...

View articletitled, MEGF9: a novel transmembrane protein with a strong and developmentally regulated expression in the nervous system

Includes: Supplementary data

Articles

Wild-type and mutant ferroportins do not form oligomers in transfected cells

Ana Sofia Gonçalves, Françoise Muzeau, Rand Blaybel, Gilles Hetet, Fathi Driss, Constance Delaby, François Canonne-Hergaux, Carole Beaumont

Biochem J (2006) 396 (2): 265–275.

https://doi.org/10.1042/BJ20051682

Published: 15 May 2006

... associated with the endoplasmic reticulum and with Rab5-positive vesicles. However, this mutant is complex-glycosylated like the wt protein. D157G and G323V mutants have a defective iron export capacity as judged by their inability to deplete the intracellular ferritin content, whereas Q182H and delV162 have...

View articletitled, Wild-type and mutant ferroportins do not form oligomers in transfected cells

Includes: Supplementary data

Articles

rhEPO (recombinant human eosinophil peroxidase): expression in Pichia pastoris and biochemical characterization

Chiara Ciaccio, Alessandra Gambacurta, Giampiero DE Sanctis, Domenico Spagnolo, Christina Sakarikou, Giovanni Petrella, Massimo Coletta

Biochem J (2006) 395 (2): 295–301.

https://doi.org/10.1042/BJ20051385

Published: 28 March 2006

... rhEPO coding sequence was cloned into the pPIC9 vector in frame with the yeast α-Factor secretion signal under the transcriptional control of the AOX (acyl-CoA oxidase) promoter, and transformed into P. pastoris strain GS115. Evidence for the production of rhEPO by P. pastoris as a glycosylated dimer...

View articletitled, rhEPO (recombinant human eosinophil peroxidase): expression in Pichia pastoris and biochemical characterization

Articles

Physical and biochemical properties of mammalian DNase X proteins: non-AUG translation initiation of porcine and bovine mRNAs for DNase X

Daisuke Shiokawa, Yukari Shika, Kazuki Saito, Kosuke Yamazaki, Sei-ichi Tanuma

Biochem J (2005) 392 (3): 511–517.

https://doi.org/10.1042/BJ20051114

Published: 06 December 2005

... proteins. A sequence comparison reveals some conserved characteristics: all the mammalian DNase X proteins have an N-terminal signal peptide, a potential N -linked glycosylation site and a C-terminal hydrophobic domain. Human DNase X, ectopically expressed in HeLa S3 cells, is located in the ER...

View articletitled, Physical and biochemical properties of mammalian DNase X proteins: non-AUG translation initiation of porcine and bovine mRNAs for DNase X

Articles

NPC1 late endosomes contain elevated levels of non-esterified (‘free’) fatty acids and an abnormally glycosylated form of the NPC2 protein

Fannie W. Chen, Ronald E. Gordon, Yiannis A. Ioannou

Biochem J (2005) 390 (2): 549–561.

https://doi.org/10.1042/BJ20050236

Published: 23 August 2005

... glycosylation/deglycosylation or trafficking, since NPC2 secreted from NPC1 cells is indistinguishable from that secreted from Wt cells. Also, a greater proportion of the normally soluble cellular NPC2 protein partitions with detergent-insoluble late endosomal internal membrane domains in NPC1 vesicles...

View articletitled, NPC1 late endosomes contain elevated levels of non-esterified (‘free’) fatty acids and an abnormally glycosylated form of the NPC2 protein

Articles

Multiple biological responses are induced by glycosylation-deficient hepatocyte growth factor

Kazuhiro FUKUTA, Kunio MATSUMOTO, Toshikazu NAKAMURA

Biochem J (2005) 388 (2): 555–562.

https://doi.org/10.1042/BJ20041698

Published: 24 May 2005

...Kazuhiro FUKUTA; Kunio MATSUMOTO; Toshikazu NAKAMURA HGF (hepatocyte growth factor), a heterodimeric glycoprotein composed of α- and β-chains, exerts biological activities through the c-Met receptor tyrosine kinase. The α-chain has three glycosylation sites, while the β-chain has two; however...

View articletitled, Multiple biological responses are induced by glycosylation-deficient hepatocyte growth factor

Includes: Supplementary data

Articles

Separation of native prion protein (PrP) glycoforms by copper-binding using immobilized metal affinity chromatography (IMAC)

Henrik MÜLLER, Alexander STROM, Gerhard HUNSMANN, Andreas W. STUKE

Biochem J (2005) 388 (1): 371–378.

https://doi.org/10.1042/BJ20041291

Published: 10 May 2005

...-linked glycosylation sites and binds copper under physiological conditions. In contrast with PrP C , PrP Sc is insoluble in non-ionic detergents and does not bind to Cu 2+ ions. Hence, we utilized copper binding to separate and characterize both PrP isoforms. Infected and uninfected murine brain...

View articletitled, Separation of native prion protein (PrP) glycoforms by copper-binding using immobilized metal affinity chromatography (IMAC)

Articles

Mucin glycosylation changes in cystic fibrosis lung disease are not manifest in submucosal gland secretions

Benjamin L. SCHULZ, Andrew J. SLOANE, Leanne J. ROBINSON, Lucille T. SEBASTIAN, Allan R. GLANVILLE, Yuanlin SONG, Alan S. VERKMAN, Jenny L. HARRY, Nicolle H. PACKER, Niclas G. KARLSSON

Biochem J (2005) 387 (3): 911–919.

https://doi.org/10.1042/BJ20041641

Published: 26 April 2005

... immunity in the lung, and have been reported to be altered in lung disease. Changes in lung mucosal glycosylation have been reported in CF (cystic fibrosis), which may be responsible for differential bacterial binding to glycosylated components in the lung mucosa and hence increased pre-disposition...

View articletitled, Mucin glycosylation changes in cystic fibrosis lung disease are not manifest in submucosal gland secretions

Includes: Supplementary data

Articles

Proteolytic processing and glycosylation influence formation of porcine prion protein complexes

Krzysztof NIEZNANSKI, Marcin RUTKOWSKI, Magdalena DOMINIK, Dariusz STEPKOWSKI

Biochem J (2005) 387 (1): 93–100.

https://doi.org/10.1042/BJ20041344

Published: 22 March 2005

... revealed that most of the PrP C molecules in porcine brain extracts exist in the form of high-molecular-mass complexes (probably with other proteins). The heterogeneity of porcine PrP C , resulting from proteolytic modification and glycosylation, influences its ability to assemble into these complexes. N...

View articletitled, Proteolytic processing and glycosylation influence formation of porcine prion protein complexes

Articles

Functional studies of human intestinal alkaline sphingomyelinase by deglycosylation and mutagenesis

Jun WU, Gert H. HANSEN, Åke NILSSON, Rui-Dong DUAN

Biochem J (2005) 386 (1): 153–160.

https://doi.org/10.1042/BJ20041455

Published: 08 February 2005

...Jun WU; Gert H. HANSEN; Åke NILSSON; Rui-Dong DUAN Intestinal alk-SMase (alkaline sphingomyelinase) is an ectoenzyme related to the NPP (nucleotide phosphodiesterase) family. It has five potential N-glycosylation sites and predicated transmembrane domains at both the N- and C-termini. The amino...

View articletitled, Functional studies of human intestinal alkaline sphingomyelinase by deglycosylation and mutagenesis

Articles

Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract

Catherine ROBBE, Calliope CAPON, Bernadette CODDEVILLE, Jean-Claude MICHALSKI

Biochem J (2004) 384 (2): 307–316.

https://doi.org/10.1042/BJ20040605

Published: 23 November 2004

... of the oligosaccharide repertoire, with respect to size, diversity and expression of glycans and terminal epitopes, showed a high level of mucin-specific glycosylation: highly fucosylated glycans, found specifically in the small intestine, were mainly based on core 4 structures, GlcNAc-(β1-3)[GlcNAc(β1-6)]GalNAc...

View articletitled, Structural diversity and specific distribution of O-glycans in normal human mucins along the intestinal tract

Articles

Effects of pH on Helicobacter pylori binding to human gastric mucins: identification of binding to non-MUC5AC mucins

Sara LINDÉN, Jafar MAHDAVI, Jan HEDENBRO, Thomas BORÉN, Ingemar CARLSTEDT

Biochem J (2004) 384 (2): 263–270.

https://doi.org/10.1042/BJ20040402

Published: 23 November 2004

... 3 2004 9 7 2004 20 7 2004 20 7 2004 The Biochemical Society, London 2004 gastric mucin glycosylation Helicobacter pylori host–pathogen interaction mucosal protection pH-dependence The stomach is lined by a viscous mucus layer that acts as a barrier...

View articletitled, Effects of pH on Helicobacter pylori binding to human gastric mucins: identification of binding to non-MUC5AC mucins

Articles

Incomplete glycosylation and defective intracellular targeting of mutant solute carrier family 11 member 1 (Slc11a1)

Jacqueline K. WHITE, Abigail STEWART, Jean-Francois POPOFF, Shona WILSON, Jenefer M. BLACKWELL

Biochem J (2004) 382 (3): 811–819.

https://doi.org/10.1042/BJ20040808

Published: 07 September 2004

...Jacqueline K. WHITE; Abigail STEWART; Jean-Francois POPOFF; Shona WILSON; Jenefer M. BLACKWELL Solute carrier family 11 member 1 (Slc11a1, formerly Nramp1) is a highly glycosylated, 12 transmembrane domain protein expressed in macrophages. It resides in the membrane of late endosomes and lysosomes...

View articletitled, Incomplete glycosylation and defective intracellular targeting of mutant solute carrier family 11 member 1 (Slc11a1)

Articles

Increased levels of insulin and insulin-like growth factor-1 hybrid receptors and decreased glycosylation of the insulin receptor alpha- and beta-subunits in scrapie-infected neuroblastoma N2a cells

Daniel NIELSEN, Hanna GYLLBERG, Pernilla ÖSTLUND, Tomas BERGMAN, Katarina BEDECS

Biochem J (2004) 380 (2): 571–579.

https://doi.org/10.1042/bj20040010

Published: 01 June 2004

.... Furthermore, the decreased molecular mass of IR subunits in ScN2a cells is not caused by altered phosphorylation or proteolytic processing, but rather by altered glycosylation. Enzymic deglycosylation of immunoprecipitated IR from N2a and ScN2a cells with endoglycosidase H, peptide N-glycosidase F...

View articletitled, Increased levels of insulin and insulin-like growth factor-1 hybrid receptors and decreased glycosylation of the insulin receptor alpha- and beta-subunits in scrapie-infected neuroblastoma N2a cells

Articles

Glycosylation of the receptor guanylate cyclase C: role in ligand binding and catalytic activity

Yashoda GHANEKAR, Akhila CHANDRASHAKER, Utpal TATU, Sandhya S. VISWESWARIAH

Biochem J (2004) 379 (3): 653–663.

https://doi.org/10.1042/bj20040001

Published: 01 May 2004

... of cGMP. GC-C is expressed as differentially glycosylated forms in HEK-293 cells (human embryonic kidney-293 cells). In the present study, we show that the 145 kDa form of GC-C contains sialic acid and galactose residues and is present on the PM (plasma membrane) of cells, whereas the 130 kDa form...

View articletitled, Glycosylation of the receptor guanylate cyclase C: role in ligand binding and catalytic activity

Articles

Recombinant MUC1 mucin with a breast cancer-like O-glycosylation produced in large amounts in Chinese-hamster ovary cells

Malin BÄCKSTRÖM, Thomas LINK, Fredrik J. OLSON, Hasse KARLSSON, Rosalind GRAHAM, Gianfranco PICCO, Joy BURCHELL, Joyce TAYLOR-PAPADIMITRIOU, Thomas NOLL, Gunnar C. HANSSON

Biochem J (2003) 376 (3): 677–686.

https://doi.org/10.1042/bj20031130

Published: 15 December 2003

...-glycosylation sites in the tandem repeats, as determined by nano-liquid chromatography MS of partially deglycosylated Clostripain-digested protein. A very similar O-glycan profile and site occupancy was found in MUC1–IgG produced in the breast carcinoma cell line T47D, which has O-glycosylation typical...

View articletitled, Recombinant MUC1 mucin with a breast cancer-like O-glycosylation produced in large amounts in Chinese-hamster ovary cells

Articles

Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore

Mohabir RAMJEESINGH, Francisca UGWU, Canhui LI, Sonja DHANI, Ling Jun HUAN, Yanchun WANG, Christine E. BEAR

Biochem J (2003) 375 (3): 633–641.

https://doi.org/10.1042/bj20030774

Published: 01 November 2003

... cysteine modification dimer glycosylation second membrane-spanning domain Abbreviations used: ABC, ATP-binding cassette; CFTR, cystic fibrosis transmembrane conductance regulator; DIDS, 4,4′-di-isothiocyanostilbene-2,2′-disulphonate; MPB, N α (3-maleimidylpropionyl)biocytin; MSD, membrane...

View articletitled, Stable dimeric assembly of the second membrane-spanning domain of CFTR (cystic fibrosis transmembrane conductance regulator) reconstitutes a chloride-selective pore

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