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Keywords: glycosylation
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Biochem J (2022) 479 (17): 1743–1758.
Published: 06 September 2022
...Samuel M. Duncan; Michael A.J. Ferguson Eukaryotic protein glycosylation is mediated by glycosyl- and oligosaccharyl-transferases. Here, we describe how African trypanosomes exhibit both evolutionary conservation and significant divergence compared with other eukaryotes in how they synthesise...
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Biochem J (2017) 474 (14): 2333–2347.
Published: 03 July 2017
...Nisha Grandhi Jayaprakash; Avadhesha Surolia Glycosylation constitutes one of the most common, ubiquitous and complex forms of post-translational modification. It commences with the synthesis of the protein and plays a significant role in deciding its folded state, oligomerization and thus its...
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Biochem J (2017) 474 (9): 1481–1493.
Published: 19 April 2017
...; François Foulquier TMEM165 deficiencies lead to one of the congenital disorders of glycosylation (CDG), a group of inherited diseases where the glycosylation process is altered. We recently demonstrated that the Golgi glycosylation defect due to TMEM165 deficiency resulted from a Golgi manganese...
Includes: Supplementary data
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Biochem J (2016) 473 (20): 3451–3462.
Published: 11 October 2016
... of a glycosyltransferase capable of forming α and β linkages underlines the peculiarity of mimivirus and enforces the concept of a host-independent glycosylation machinery in mimivirus. Correspondence: Thierry Hennet ( [email protected] ) 1 4 2016 4 7 2016 18 7 2016 18 7 2016 © 2016...
Includes: Supplementary data
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Biochem J (2016) 473 (13): 1953–1965.
Published: 28 June 2016
... in serotonergic neurons and involved in the transport of small neutral amino acids across the plasma membrane. Co-expression of ASCT2 with SERT in HEK (human embryonic kidney)-293 cells affects glycosylation and cell-surface localization of SERT with a concomitant reduction in its 5-HT uptake activity. Conversely...
Includes: Supplementary data
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Biochem J (2016) 473 (11): 1471–1482.
Published: 27 May 2016
... of signalling events, zymogen activation, post-translational modifications and extracellular inhibition. Slight imbalances may result in the initiation or progression of specific disease states, such as cancer and pathological inflammation. As glycosylation modifies the structures and functions of glycoproteins...
Includes: Supplementary data
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Biochem J (2015) 472 (2): e25–e26.
Published: 13 November 2015
... of Mariappa et al. advances the field. glycosylation mass spectrometry OGA O-linked N -acetylglucosamine (O-GlcNAc) Cells employ a large selection of post-translational modifications (PTMs) to dynamically alter the activity of proteins. One of the most common is the addition of a single N...
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Biochem J (2012) 444 (3): 429–435.
Published: 29 May 2012
... membrane to the host nuclear membrane during viral replication. Given the abundance of HA on the viral surface, this protein is also the primary target for host innate and adaptive immune responses. Although addition of glycosylation sites on HA are a part of viral evolution to evade the host immune...
Includes: Supplementary data
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Biochem J (2012) 443 (2): 427–437.
Published: 27 March 2012
...-acetyltransferase (GNA) glycosylation N -acetylglucosamine biosynthesis nucleotide sugar GlcNAc ( N -acetylglucosamine or 2-acetamido-2-deoxy-β- D -glucopyranose) is an acetylated amino sugar that is ubiquitous in all clades of life. GlcNAc occurs in structural polymers such as chitin, which is one...
Includes: Supplementary data
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Biochem J (2011) 436 (2): 263–269.
Published: 13 May 2011
... inhibited maturation of LRP6 by controlling the glycosylation of LRP6. Knockdown of Mest/Peg1, which might enhance Wnt signalling, blocked adipogenic differentiation of 3T3-L1 cells. Overall, our results suggest that Mest/Peg1 is a novel regulator of Wnt/β-catenin signalling during adipogenic...
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Biochem J (2011) 435 (3): 609–618.
Published: 13 April 2011
... growth factor), and to identify the signalling mechanisms involved. NRP1 is highly expressed in HAoSMCs (human aortic smooth muscle cells) and HCASMCs, and modified in VSMCs by CS (chondroitin sulfate)-rich O-linked glycosylation at Ser 612 . HCASMC migration induced by PDGF-BB and PDGF-AA was inhibited...
Includes: Supplementary data
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Biochem J (2011) 434 (1): 61–72.
Published: 27 January 2011
... with intracellular pH, with increased activity at low intracellular pH. Maximal pendrin activity was also stimulated at low extracellular pH, suggesting the presence of both intracellular and extracellular proton regulatory sites. We identified five putative pendrin glycosylation sites, only two of which are used...
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Biochem J (2010) 429 (2): 359–367.
Published: 28 June 2010
... corresponding to the sequence NeuAcα2–3Galβ1–3(NeuAcα2–3Galβ1–3/4GlcNAcβ1–6)GalNAc. It was also found that a small proportion of the core 2 oligosaccharides carried sulfate. The ability of lubricin to present complex glycosylation reflecting the state of the joint tissue makes lubricin a candidate as a carrier...
Includes: Supplementary data
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Biochem J (2009) 417 (1): 77–83.
Published: 12 December 2008
... compilation © 2009 Biochemical Society 2009 fragment crystallizable (Fc) glycosylation immunoglobulin G (IgG) rabbit ( Oryctolagus cuniculus ) The rabbit ( Oryctolagus cuniculus ) immunoglobulin gamma, IgG, was one of the first molecules of the immune system to be investigated...
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Biochem J (2008) 416 (2): e11–e13.
Published: 12 November 2008
... of the authors (email [email protected] or [email protected] ). 15 10 2008 17 10 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 glucose sensing glycosylation hexosamine pathway metabolism Wnt signalling Coupling nutrient availability, prioritization...
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Biochem J (2007) 403 (1): 97–108.
Published: 13 March 2007
..., the electron transferase of the phagocyte Nox. In vitro Nox3 cDNA expressed an ∼50 kDa primary translation product that underwent N-linked glycosylation in the presence of canine microsomes. RNAi (RNA interference)-mediated reduction of endogenous p22 phox , a subunit essential for stabilization of Nox2...
Includes: Supplementary data
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Biochem J (2007) 402 (3): 515–523.
Published: 26 February 2007
... palmitoylation of Wnt-5a. These results indicate that palmitoylation of Wnt-5a is important for the triggering of signalling at the cell surface level and, therefore, that the lipid-unmodified form of Wnt-5a cannot activate intracellular signal cascades. In contrast, glycosylation was necessary for the secretion...
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Biochem J (2007) 401 (2): 447–457.
Published: 21 December 2006
... nervous system). The domain structure of MEGF9 consists of an N-terminal region with several potential O-glycosylation sites followed by five EGF-like domains, which are highly homologous with the short arms of laminins. Following one single pass transmembrane domain, a highly conserved short...
Includes: Supplementary data
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Biochem J (2006) 396 (2): 265–275.
Published: 15 May 2006
... associated with the endoplasmic reticulum and with Rab5-positive vesicles. However, this mutant is complex-glycosylated like the wt protein. D157G and G323V mutants have a defective iron export capacity as judged by their inability to deplete the intracellular ferritin content, whereas Q182H and delV162 have...
Includes: Supplementary data
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Biochem J (2006) 395 (2): 295–301.
Published: 28 March 2006
... rhEPO coding sequence was cloned into the pPIC9 vector in frame with the yeast α-Factor secretion signal under the transcriptional control of the AOX (acyl-CoA oxidase) promoter, and transformed into P. pastoris strain GS115. Evidence for the production of rhEPO by P. pastoris as a glycosylated dimer...
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Biochem J (2005) 388 (2): 555–562.
Published: 24 May 2005
...Kazuhiro FUKUTA; Kunio MATSUMOTO; Toshikazu NAKAMURA HGF (hepatocyte growth factor), a heterodimeric glycoprotein composed of α- and β-chains, exerts biological activities through the c-Met receptor tyrosine kinase. The α-chain has three glycosylation sites, while the β-chain has two; however...
Includes: Supplementary data
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Biochem J (2005) 388 (1): 371–378.
Published: 10 May 2005
...-linked glycosylation sites and binds copper under physiological conditions. In contrast with PrP C , PrP Sc is insoluble in non-ionic detergents and does not bind to Cu 2+ ions. Hence, we utilized copper binding to separate and characterize both PrP isoforms. Infected and uninfected murine brain...
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Biochem J (2005) 387 (1): 93–100.
Published: 22 March 2005
... revealed that most of the PrP C molecules in porcine brain extracts exist in the form of high-molecular-mass complexes (probably with other proteins). The heterogeneity of porcine PrP C , resulting from proteolytic modification and glycosylation, influences its ability to assemble into these complexes. N...
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Biochem J (2005) 386 (1): 153–160.
Published: 08 February 2005
...Jun WU; Gert H. HANSEN; Åke NILSSON; Rui-Dong DUAN Intestinal alk-SMase (alkaline sphingomyelinase) is an ectoenzyme related to the NPP (nucleotide phosphodiesterase) family. It has five potential N-glycosylation sites and predicated transmembrane domains at both the N- and C-termini. The amino...
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Biochem J (2004) 384 (2): 307–316.
Published: 23 November 2004
... of the oligosaccharide repertoire, with respect to size, diversity and expression of glycans and terminal epitopes, showed a high level of mucin-specific glycosylation: highly fucosylated glycans, found specifically in the small intestine, were mainly based on core 4 structures, GlcNAc-(β1-3)[GlcNAc(β1-6)]GalNAc...
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Biochem J (2004) 379 (3): 653–663.
Published: 01 May 2004
... of cGMP. GC-C is expressed as differentially glycosylated forms in HEK-293 cells (human embryonic kidney-293 cells). In the present study, we show that the 145 kDa form of GC-C contains sialic acid and galactose residues and is present on the PM (plasma membrane) of cells, whereas the 130 kDa form...
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