... in the GT-27 family of glycosyltransferases. Substrate preference across different isoforms of this enzyme is influenced by isoform-specific amino acid sequences at the site of glycosylation, which we have exploited to engineer production of Core 1 glycan structures in bacteria on human therapeutic proteins...

... 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 enzyme fucosylation FUT8 glycosyltransferase substrate specificity The α1,6-fucosyltransferase, FUT8, is the sole enzyme responsible for the core-fucosylation of N -glycans...

... with five recordings. glycosyltransferase structural reshuffling T6P Tps1 trehalose Trehalose, a non-reducing disaccharide, has been found in prokaryotes, low eukaryotes, plants, invertebrates, but not vertebrates [ 1 ]. Trehalose can be used to counteract environmental stresses...

... linked to UDP, inhibiting glycosyltransfer on to protein and peptide substrates. bisubstrate analogue inhibitor glycosyltransferase O-GlcNAc rational drug design Reversible post-translational modification of nuclear and cytoplasmic proteins with β-linked O-GlcNAc in metazoa is involved...

... carbohydrate antigenic factor, the β-mannans. 1 These authors contributed equally to this work. 2 To whom correspondence should be addressed (email [email protected] ). 31 7 2013 4 10 2013 21 10 2013 21 10 2013 cell wall glycosyltransferase β-mannose...

...Jakub Kopycki; Elizabeth Wieduwild; Janine Kohlschmidt; Wolfgang Brandt; Anna N. Stepanova; Jose M. Alonso; M. Soledade C. Pedras; Steffen Abel; C. Douglas Grubb Plant genomes encode numerous small molecule glycosyltransferases which modulate the solubility, activity, immunogenicity...

... be addressed (email [email protected] ). 11 8 2011 19 9 2011 27 9 2011 27 9 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 chondroitin sulfate glycosaminoglycan glycosyltransferase proteoglycan sulfotransferase CSs (chondroitin...

[email protected] ). 10 9 2010 29 11 2010 8 12 2010 8 12 2010 © The Authors Journal compilation © 2011 Biochemical Society 2011 chondroitin sulfate glycosaminoglycan glycosyltransferase proteoglycan sulfotransferase CS (chondroitin sulfate) is a class of GAG...

... therefore investigated whether SOX9 transduction of cultured human articular chondrocytes had any effect on the gene expression of the glycosyltransferases and sulfotransferases necessary for GAG biosynthesis. Retroviral SOX9 transduction of passaged chondrocytes increased the endogenous rate of GAG...

... and localization of this and other glycosyltransferases are still poorly characterized. Considering the possibility that interactions with other proteins may influence these processes, in the present study we carried out a yeast two-hybrid screening using elements of the N-terminal domain of GalT2 as bait...

...Tomomi Izumikawa; Toru Uyama; Yuka Okuura; Kazuyuki Sugahara; Hiroshi Kitagawa Previously, we have demonstrated that co-expression of ChSy-1 (chondroitin synthase-1), with ChPF (chondroitin-polymerizing factor) resulted in a marked augmentation of glycosyltransferase activities and the expression...

...Christiane Goedl; Richard Griessler; Alexandra Schwarz; Bernd Nidetzky The cDNA encoding trehalose phosphorylase, a family GT-4 glycosyltransferase from the fungus Schizophyllum commune , was isolated and expressed in Escherichia coli to yield functional recombinant protein in its full length...

... the appropriate sugar donor to an activated lipid carrier by committed GTs (glycosyltransferases). While the nucleotide sequence of many cps loci is already known, the real substrate specificity of the hypothetical GTs, as well as the sequence of sugar addition is unclear. In the present paper, we report...

...Peter BENCÚR; Herta STEINKELLNER; Barbara SVOBODA; Jan MUCHA; Richard STRASSER; Daniel KOLARICH; Stephan HANN; Gunda KÖLLENSPERGER; Josef GLÖSSL; Friedrich ALTMANN; Lukas MACH XylT (β1,2-xylosyltransferase) is a unique Golgi-bound glycosyltransferase that is involved in the biosynthesis...

... 2004 The Biochemical Society, London 2005 Corynebacterium callunae glycogen phosphorylase glycosyltransferase maltodextrin retaining mechanism site-directed mutagenesis Glycogen phosphorylases (GPs; EC 2.4.1.1) constitute a large family of GTs (glycosyltransferases) and occur...

... cloned a cDNA (CG17223) from Drosophila melanogaster encoding a protein with homology to mammalian α1,4-glycosyltransferases and expressed it in the yeast Pichia pastoris . Culture supernatants from the transformed yeast were found to display a novel UDP-GalNAc:GalNAcβ1,4GlcNAcβ1-R α- N...

... the two organelles. Using exogenous acceptors coated on silica gel, we demonstrate the presence of ceramide glucosyltransferase (Cer-Glc-T), glucosylceramide galactosyltransferase and sialyltransferase (SAT) activities in the MAM. Estimation of the marker-enzyme activities showed that glycosyltransferase...

... AF401657. 30 10 2001 11 2 2002 15 4 2002 The Biochemical Society, London ©2002 2002 glycosyltransferase glucuronidation steroid metabolism relative activity genomic structure Abbreviations used: UDPGA, uridine diphosphoglucuronic acid; UGT, uridine...

... phosphate; GT, glycosyltransferase; S N i, internal return. family 4 glycosyltransferase intermediate retaining mechanism Biochem. J. (2001) 360, 727 736 (Printed in Great Britain) 727 a-Retaining glucosyl transfer catalysed by trehalose phosphorylase from Schizophyllum commune : mechanistic...

... endothelial cell interactions during inflammation, tumour cell endothelial cell inter- actions during metastasis [2], and adhesion of pathogenic agents to their host cells [3]. In cancer, fucosyltransferases, as well as other glycosyltransferases, are highly expressed, resulting in the overexpression...

[email protected] ). 14 8 2000 16 10 2000 6 11 2000 The Biochemical Society, London © 2001 2001 glycosyltransferase insect cell Lewis determinants Biochem. J. (2001) 353, 719 725 (Printed in Great Britain) 719 Expression and characterization of recombinant human a-3/4...

... of polymorphism between specimens. This intra-specific variability was restricted to the terminal substitution of O-linked oligosaccharides. The differential expression of two glycosyltransferase [an α-(1 → 4) galactosyltransferase and an α-(1 → 3) fucosyltransferase] activities resulted in the characterization...

... family of nucleotide-sugar-utilizing glycosyltransferases. To establish whether Gpi3p is the UDP-GlcNAc-binding subunit of the yeast GlcNAc-PI synthetic complex, we tested its ability to become cross-linked to the photoactivatable substrate analogue P 3 -(4-azidoanilido)-uridine 5´-triphosphate (AAUTP...

... submitted to the EMBL, GenBank ® and DDBJ Nucleotide Sequence Databases under the accession number AF047707. 5 3 1999 19 4 1999 11 5 1999 The Biochemical Society, London © 1999 1999 diethyl pyrocarbonate glycosphingolipids glycosyltransferase Golgi apparatus, D -threo-1...

... glucuronidation steroid catabolism glycosyltransferase Biochem. J. (1999) 337, 567 574 (Printed in Great Britain) 567 Cloning and characterization of a simian UDP-glucuronosyltransferase enzyme UGT2B20, a novel C19 steroid-conjugating protein Olivier BARBIER*, Alain BE; LANGER*‹ and Dean W. HUM*1...