... a nucleophilic water molecule for hydrolysis. The D139A, D143A and E555A mutants significantly decreased hydrolytic activity, indicating their essential role. Of these mutants, D139A exclusively exhibited glycosynthase activity using α-GlcN-F (α- D -glucosaminyl fluoride) and GlcN as substrates, to produce (GlcN...

... activity of their cognate catalytic domains by increasing enzyme substrate proximity. Mimicking their role in Nature, we, in the present study, propose that CBMs may assist in vitro glycosynthase-catalysed polymerization reactions to produce artificial polysaccharides. Glycosynthases are GHs that have been...

...Eduardo Andrés; Hugo Aragunde; Antoni Planas Glycosynthases have become efficient tools for the enzymatic synthesis of oligosaccharides, glycoconjugates and polysaccharides. Enzyme-directed evolution approaches are applied to improve the performance of current glycosynthases and engineer...

... BcChi-A into a glycosynthase, we employed the strategy reported by Honda et al. [(2006) J. Biol. Chem. 281 , 1426–1431; (2008) Glycobiology 18 , 325–330] of mutating Ser 102 , which holds a nucleophilic water molecule, and Glu 70 , which acts as a catalytic base, producing S102A, S102C, S102D, S102G...

... that such syntheses are possible using genetically modified glycoside hydrolases, i.e. glycosynthases. The ability of the endoglycosynthase derived from Bacillus (1→3,1→4)-β- d -glucanase to catalyse self-condensation of sugar donors was exploited for the in vitro synthesis of a regular polysaccharide...

...Andrew G. WATTS; Stephen G. WITHERS Glycosynthases are mutant glycosidases, genetically engineered to catalyse the synthesis of oligosaccharides. A study by Faijes et al. in this issue of the Biochemical Journal has expanded the ability of glycosynthases to catalyse the polymerization...

... 17 1 2001 The Biochemical Society, London © 2001 2001 chemical rescue covalent intermediate glycosidase mechanism glycosynthase 1,3-1,4-β-glucanase Biochem. J. (2001) 355, 79 86 (Printed in Great Britain) 79 Long-lived glycosyl-enzyme intermediate mimic produced by formate re...