...Alaa Shafie; Mingzhan Xue; Guy Barker; Daniel Zehnder; Paul J. Thornalley; Naila Rabbani Glyoxalase 1 (Glo1) is a cytoplasmic enzyme with a cytoprotective function linked to metabolism of the cytotoxic side product of glycolysis, methylglyoxal (MG). It prevents dicarbonyl stress — the abnormal...

...Marta Sousa Silva; Ricardo A. Gomes; Antonio E. N. Ferreira; Ana Ponces Freire; Carlos Cordeiro The discovery of the enzymatic formation of lactic acid from methylglyoxal dates back to 1913 and was believed to be associated with one enzyme termed ketonaldehydemutase or glyoxalase, the latter...

... damage is countered by glutathione-dependent metabolism by Glo1 (glyoxalase 1). It is not known, however, whether Glo1 has stress-responsive up-regulation to counter periods of high MG concentration or dicarbonyl stress. We identified a functional ARE (antioxidant-response element) in the 5′-untranslated...

...Neil Greig; Susan Wyllie; Tim J. Vickers; Alan H. Fairlamb The glyoxalase system, comprizing glyoxalase I and glyoxalase II, is a ubiquitous pathway that detoxifies highly reactive aldehydes, such as methylglyoxal, using glutathione as a cofactor. Recent studies of Leishmania major glyoxalase I...

...Nicole SUKDEO; Susan L. CLUGSTON; Elisabeth DAUB; John F. HONEK The metalloisomerase glyoxalase I (GlxI) catalyses the conversion of methylglyoxal-glutathione hemithioacetal and related derivatives into the corresponding thioesters. In contrast with the previously characterized GlxI enzymes of Homo...