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Articles

Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach

Halina Wojtowicz, Ada Prochnicka-Chalufour, Gisele Cardoso de Amorim, Olga Roudenko, Catherine Simenel, Idir Malki, Gérard Pehau-Arnaudet, Francesca Gubellini, Alexandros Koutsioubas, Javier Pérez, Philippe Delepelaire, Muriel Delepierre, Rémi Fronzes, Nadia Izadi-Pruneyre

Biochem J (2016) 473 (14): 2239–2248.

https://doi.org/10.1042/BCJ20160131

Published: 12 July 2016

.... Although proteins involved in this process have been identified, signal transduction through this family of transporters is not well understood. In the present study, using an integrative approach (EM, SAXS, X-ray crystallography and NMR), we have studied the structure of the haem transporter HasR captured...

View articletitled, Structural basis of the signalling through a bacterial membrane receptor HasR deciphered by an integrative approach

Includes: Supplementary data

Articles

Dissecting structural and electronic effects in inducible nitric oxide synthase

Luciana Hannibal, Richard C. Page, Mohammad Mahfuzul Haque, Karthik Bolisetty, Zhihao Yu, Saurav Misra, Dennis J. Stuehr

Biochem J (2015) 467 (1): 153–165.

https://doi.org/10.1042/BJ20141319

Published: 20 March 2015

... B have been deposited in the PDB under code 4JS9. 29 10 2014 23 12 2014 22 1 2015 22 1 2015 © The Authors Journal compilation © 2015 Biochemical Society 2015 Nitric oxide synthases (NOSs) are haem-thiolate enzymes that catalyse the conversion of L -arginine ( L...

View articletitled, Dissecting structural and electronic effects in inducible nitric oxide synthase

Includes: Supplementary data

Articles

Mutation of Trp 93 of MauG to tyrosine causes loss of bound Ca 2+ and alters the kinetic mechanism of tryptophan tryptophylquinone cofactor biosynthesis

Sooim Shin, Manliang Feng, Victor L. Davidson

Biochem J (2013) 456 (1): 129–137.

https://doi.org/10.1042/BJ20130981

Published: 24 October 2013

... is positioned midway between its two haems, and in close proximity to a Ca 2+ that is critical for MauG function. Mutation of Trp 93 to tyrosine caused loss of bound Ca 2+ and changes in spectral features similar to those observed after removal of Ca 2+ from WT (wild-type) MauG. However, whereas Ca 2+ -depleted...

View articletitled, Mutation of Trp 93 of MauG to tyrosine causes loss of bound Ca 2+ and alters the kinetic mechanism of tryptophan tryptophylquinone cofactor biosynthesis

Articles

Unexpected weak magnetic exchange coupling between haem and non-haem iron in the catalytic site of nitric oxide reductase (NorBC) from Paracoccus denitrificans

Jessica H. Van Wonderen, Vasily S. Oganesyan, Nicholas J. Watmough, David J. Richardson, Andrew J. Thomson, Myles R. Cheesman

Biochem J (2013) 451 (3): 389–394.

https://doi.org/10.1042/BJ20121406

Published: 12 April 2013

...Jessica H. Van Wonderen; Vasily S. Oganesyan; Nicholas J. Watmough; David J. Richardson; Andrew J. Thomson; Myles R. Cheesman Bacterial NOR (nitric oxide reductase) is a major source of the powerful greenhouse gas N 2 O. NorBC from Paracoccus denitrificans is a heterodimeric multi-haem...

View articletitled, Unexpected weak magnetic exchange coupling between haem and non-haem iron in the catalytic site of nitric oxide reductase (NorBC) from Paracoccus denitrificans

Includes: Supplementary data

Articles

Ferroportin expression in haem oxygenase 1-deficient mice

Rafał R. Starzyński, François Canonne-Hergaux, Małgorzata Lenartowicz, Wojciech Krzeptowski, Alexandra Willemetz, Agnieszka Styś, Joanna Bierła, Piotr Pietrzak, Tomasz Dziaman, Paweł Lipiński

Biochem J (2013) 449 (1): 69–78.

https://doi.org/10.1042/BJ20121139

Published: 07 December 2012

...Rafał R. Starzyński; François Canonne-Hergaux; Małgorzata Lenartowicz; Wojciech Krzeptowski; Alexandra Willemetz; Agnieszka Styś; Joanna Bierła; Piotr Pietrzak; Tomasz Dziaman; Paweł Lipiński HO1 (haem oxygenase 1) and Fpn (ferroportin) are key proteins for iron recycling from senescent red blood...

View articletitled, Ferroportin expression in haem oxygenase 1-deficient mice

Includes: Supplementary data

Articles

Probing why trypanosomes assemble atypical cytochrome c with an AxxCH haem-binding motif instead of CxxCH

Michael L. Ginger, Katharine A. Sam, James W. A. Allen

Biochem J (2012) 448 (2): 253–260.

https://doi.org/10.1042/BJ20120757

Published: 07 November 2012

...Michael L. Ginger; Katharine A. Sam; James W. A. Allen Mitochondrial cytochromes c and c 1 are core components of the respiratory chain of all oxygen-respiring eukaryotes. These proteins contain haem, covalently bound to the polypeptide in a catalysed post-translational modification. In all...

View articletitled, Probing why trypanosomes assemble atypical cytochrome c with an AxxCH haem-binding motif instead of CxxCH

Includes: Supplementary data

Articles

The crystal structure of human α 1 -microglobulin reveals a potential haem-binding site

Winfried Meining, Arne Skerra

Biochem J (2012) 445 (2): 175–182.

https://doi.org/10.1042/BJ20120448

Published: 27 June 2012

...’. However, the three side chains of Lys 92 , Lys 118 and Lys 130 that were reported to be involved in covalent chromophore binding appear to be freely accessible to ligands accommodated in the hydrophobic pocket. A structural feature similar to the well-known Cys–Pro haem-binding motif indicates...

View articletitled, The crystal structure of human α 1 -microglobulin reveals a potential haem-binding site

Includes: Supplementary data

Articles

Lactococcus lactis HemW (HemN) is a haem-binding protein with a putative role in haem trafficking

Helge K. Abicht, Jacobo Martinez, Gunhild Layer, Dieter Jahn, Marc Solioz

Biochem J (2012) 442 (2): 335–343.

https://doi.org/10.1042/BJ20111618

Published: 13 February 2012

...Helge K. Abicht; Jacobo Martinez; Gunhild Layer; Dieter Jahn; Marc Solioz Lactococcus lactis cannot synthesize haem, but when supplied with haem, expresses a cytochrome bd oxidase. Apart from the cydAB structural genes for this oxidase, L. lactis features two additional genes, hemH and hemW ( hemN...

View articletitled, Lactococcus lactis HemW (HemN) is a haem-binding protein with a putative role in haem trafficking

Includes: Supplementary data

Articles

Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Heeyong Yoon, Ramesh Golla, Emmanuel Lesuisse, Jayashree Pain, Jason E. Donald, Elise R. Lyver, Debkumar Pain, Andrew Dancis

Biochem J (2012) 441 (1): 473–480.

https://doi.org/10.1042/BJ20111637

Published: 14 December 2011

... were returned to normal and haem synthesis was restored. In mitochondria carrying the mutant Isu1 and no frataxin, Fe–S cluster enzyme activities were improved. The efficiency of new Fe–S cluster synthesis in isolated mitochondria was markedly increased compared with frataxin-negative cells, although...

View articletitled, Mutation in the Fe–S scaffold protein Isu bypasses frataxin deletion

Articles

Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Heeyong Yoon, Yan Zhang, Jayashree Pain, Elise R. Lyver, Emmanuel Lesuisse, Debkumar Pain, Andrew Dancis

Biochem J (2011) 440 (1): 137–146.

https://doi.org/10.1042/BJ20111036

Published: 27 October 2011

...Heeyong Yoon; Yan Zhang; Jayashree Pain; Elise R. Lyver; Emmanuel Lesuisse; Debkumar Pain; Andrew Dancis Mitochondria transport and utilize iron for the synthesis of haem and Fe–S clusters. Although many proteins are known to be involved in these processes, additional proteins are likely...

View articletitled, Rim2, a pyrimidine nucleotide exchanger, is needed for iron utilization in mitochondria

Articles

Entamoeba histolytica secretes two haem-binding proteins to scavenge haem

Areli Cruz-Castañeda, Mavil López-Casamichana, José J. Olivares-Trejo

Biochem J (2011) 434 (1): 105–111.

https://doi.org/10.1042/BJ20100897

Published: 27 January 2011

... of supporting its growth in the presence of haem as the sole iron supply. In addition, when trophozoites were maintained in cultures supplemented with haemoglobin as the only iron source, the haem was released and thus it was introduced into cells. Interestingly, the Ehhmbp26 and Ehhmbp45 proteins could...

View articletitled, Entamoeba histolytica secretes two haem-binding proteins to scavenge haem

Articles

Role of the cysteine protease interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin

Dominic P. Byrne, Katarzyna Wawrzonek, Anna Jaworska, Andrew J. Birss, Jan Potempa, John W. Smalley

Biochem J (2010) 425 (1): 257–264.

https://doi.org/10.1042/BJ20090343

Published: 14 December 2009

... A), a homologue of Streptococcus pyogenes streptopain (SpeB). The role of InpA in haemoglobin breakdown and haem release was investigated. At pH 7.5, InpA mediated oxidation of oxyhaemoglobin to hydroxymethaemoglobin [in which the haem iron is oxidized to the Fe(III) state and which carries OH − as the sixth co...

View articletitled, Role of the cysteine protease interpain A of Prevotella intermedia in breakdown and release of haem from haemoglobin

Articles

The steady-state mechanism of cytochrome c oxidase: redox interactions between metal centres

Maria G. Mason, Peter Nicholls, Chris E. Cooper

Biochem J (2009) 422 (2): 237–246.

https://doi.org/10.1042/BJ20082220

Published: 13 August 2009

...Maria G. Mason; Peter Nicholls; Chris E. Cooper The steady-state behaviour of isolated mammalian cytochrome c oxidase was examined by increasing the rate of reduction of cytochrome c . Under these conditions the enzyme's 605 (haem a ), 655 (haem a 3 /Cu B ) and 830 (Cu A ) nm spectral features...

View articletitled, The steady-state mechanism of cytochrome c oxidase: redox interactions between metal centres

Includes: Supplementary data

Articles

Structure of human porphobilinogen deaminase at 2.8 Å: the molecular basis of acute intermittent porphyria

Raj Gill, Simon E. Kolstoe, Fiyaz Mohammed, Abeer Al d-Bass, Julie E. Mosely, Mohammed Sarwar, Jonathan B. Cooper, Stephen P. Wood, Peter M. Shoolingin-Jordan

Biochem J (2009) 420 (1): 17–25.

https://doi.org/10.1042/BJ20082077

Published: 28 April 2009

... Figure S1 at http://www.BiochemJ.org/bj/420/bj420017add.htm ). For example, there is 60% similarity and 43% identity between the deaminases from Escherichia coli and humans [ 6 ] (see Figure 1 ). PBGD from E. coli was the first haem biosynthesis enzyme structure to be solved, initially at 1.90 Å (1...

View articletitled, Structure of human porphobilinogen deaminase at 2.8 Å: the molecular basis of acute intermittent porphyria

Includes: Supplementary data

Articles

Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins

Simon Messner, Stephan Leitner, Christian Bommassar, Georg Golderer, Peter Gröbner, Ernst R. Werner, Gabriele Werner-Felmayer

Biochem J (2009) 418 (3): 691–700.

https://doi.org/10.1042/BJ20080192

Published: 25 February 2009

... is comparable with various animal NOSs, none of the exon–intron boundaries are conserved. Recombinant expression of clones with various N-termini identified N-terminal amino acids essential for enzyme activity, but not required for haem binding or dimerization, and suggests the usage of non-AUG start codons...

View articletitled, Physarum nitric oxide synthases: genomic structures and enzymology of recombinant proteins

Articles

New insights into the role of Fur proteins: FurB ( All2473 ) from Anabaena protects DNA and increases cell survival under oxidative stress

Sara López-Gomollón, Emma Sevilla, M. Teresa Bes, M. Luisa Peleato, María F. Fillat

Biochem J (2009) 418 (1): 201–207.

https://doi.org/10.1042/BJ20081066

Published: 28 January 2009

... are frequently found in the same organism. The genome of the filamentous cyanobacterium Anabaena ( Nostoc ) sp. PCC 7120 codes for three different fur genes. FurA is an essential protein involved in iron homoeostasis that also modulates dinitrogen fixation. FurA interacts with haem, impairing its DNA-binding...

View articletitled, New insights into the role of Fur proteins: FurB ( All2473 ) from Anabaena protects DNA and increases cell survival under oxidative stress

Includes: Supplementary data

Articles

Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae

Robert N. Campbell, Michael K. Leverentz, Louise A. Ryan, Richard J. Reece

Biochem J (2008) 414 (2): 177–187.

https://doi.org/10.1042/BJ20080923

Published: 12 August 2008

... an oxidoreductase enzyme. 1 To whom correspondence should be addressed (email [email protected] ). 13 5 2008 11 6 2008 12 6 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 galactose glucose haem meiosis oxygen The Zn(II) 2 Cys 6...

View articletitled, Metabolic control of transcription: paradigms and lessons from Saccharomyces cerevisiae

Articles

The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection

Hans-Petter Hersleth, Ya-Wen Hsiao, Ulf Ryde, Carl Henrik Görbitz, K. Kristoffer Andersson

Biochem J (2008) 412 (2): 257–264.

https://doi.org/10.1042/BJ20070921

Published: 14 May 2008

... be addressed (email [email protected] ). 12 7 2007 22 1 2008 23 1 2008 23 1 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 cryoradiolytic haem iron myoglobin oxygen binding peroxide complex Mb (myoglobin) is a haem protein...

View articletitled, The crystal structure of peroxymyoglobin generated through cryoradiolytic reduction of myoglobin compound III during data collection

Articles

Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

Constance Delaby, Nathalie Pilard, Hervé Puy, François Canonne-Hergaux

Biochem J (2008) 411 (1): 123–131.

https://doi.org/10.1042/BJ20071474

Published: 13 March 2008

...Constance Delaby; Nathalie Pilard; Hervé Puy; François Canonne-Hergaux Tissue macrophages play an essential role in iron recycling through the phagocytosis of senescent RBCs (red blood cells). Following haem catabolism by HO1 (haem oxygenase 1), they recycle iron back into the plasma through...

View articletitled, Sequential regulation of ferroportin expression after erythrophagocytosis in murine macrophages: early mRNA induction by haem, followed by iron-dependent protein expression

Includes: Supplementary data

Articles

Voltammetric characterization of the aerobic energy-dissipating nitrate reductase of Paracoccus pantotrophus : exploring the activity of a redox-balancing enzyme as a function of electrochemical potential

Andrew J. Gates, David J. Richardson, Julea N. Butt

Biochem J (2008) 409 (1): 159–168.

https://doi.org/10.1042/BJ20071088

Published: 11 December 2007

... Society 2008 but they are distinguished by their cellular location, biochemistry and pattern of expression ( Figure 1 ). Nar is expressed during anaerobic growth in the presence of nitrate. NarI, the membrane spanning di-haem subunit, acquires the electrons required for nitrate reduction from...

View articletitled, Voltammetric characterization of the aerobic energy-dissipating nitrate reductase of Paracoccus pantotrophus : exploring the activity of a redox-balancing enzyme as a function of electrochemical potential

Includes: Supplementary data

Articles

Reactivity and endogenous modification by nitrite and hydrogen peroxide: does human neuroglobin act only as a scavenger?

Stefania Nicolis, Enrico Monzani, Chiara Ciaccio, Paolo Ascenzi, Luc Moens, Luigi Casella

Biochem J (2007) 407 (1): 89–99.

https://doi.org/10.1042/BJ20070372

Published: 12 September 2007

...Stefania Nicolis; Enrico Monzani; Chiara Ciaccio; Paolo Ascenzi; Luc Moens; Luigi Casella NGB (human neuroglobin), a recently discovered haem protein of the globin family containing a six-co-ordinated haem, is expressed in nervous tissue, but the physiological function of NGB is currently unknown...

View articletitled, Reactivity and endogenous modification by nitrite and hydrogen peroxide: does human neuroglobin act only as a scavenger?

Includes: Supplementary data

Articles

Interactions between substrates and the haem-bound nitric oxide of ferric and ferrous bacterial nitric oxide synthases

François J. M. Chartier, Manon Couture

Biochem J (2007) 401 (1): 235–245.

https://doi.org/10.1042/BJ20060913

Published: 11 December 2006

...François J. M. Chartier; Manon Couture We report here the resonance Raman spectra of the FeIII–NO and FeII–NO complexes of the bacterial NOSs (nitric oxide synthases) from Staphylococcus aureus and Bacillus subtilis . The haem–NO complexes of these bacterial NOSs displayed Fe–N–O frequencies...

View articletitled, Interactions between substrates and the haem-bound nitric oxide of ferric and ferrous bacterial nitric oxide synthases

Includes: Supplementary data

Articles

A new class of [2Fe-2S]-cluster-containing protoporphyrin (IX) ferrochelatases

Mark Shepherd, Tamara A. Dailey, Harry A. Dailey

Biochem J (2006) 397 (1): 47–52.

https://doi.org/10.1042/BJ20051967

Published: 14 June 2006

...Mark Shepherd; Tamara A. Dailey; Harry A. Dailey Protoporphyrin (IX) ferrochelatase catalyses the insertion of ferrous iron into protoporphyrin IX to form haem. These ferrochelatases exist as monomers and dimers, both with and without [2Fe-2S] clusters. The motifs for [2Fe-2S] cluster co-ordination...

View articletitled, A new class of [2Fe-2S]-cluster-containing protoporphyrin (IX) ferrochelatases

Articles

Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer–micelle association

Richard K. Hughes, Eric J. Belfield, Mylrajan Muthusamay, Anuja Khan, Arthur Rowe, Stephen E. Harding, Shirley A. Fairhurst, Stephen Bornemann, Ruth Ashton, Roger N. F. Thorneley, Rod Casey

Biochem J (2006) 395 (3): 641–652.

https://doi.org/10.1042/BJ20051667

Published: 11 April 2006

...), a recombinant plant cytochrome P450 enzyme with HPL (hydroperoxide lyase) activity from Medicago truncatula (barrel medic). Steady-state kinetic parameters, substrate and product specificities, RZ (Reinheitszahl or purity index), molar absorption coefficient, haem content, and new ligands for an HPL...

View articletitled, Characterization of Medicago truncatula (barrel medic) hydroperoxide lyase (CYP74C3), a water-soluble detergent-free cytochrome P450 monomer whose biological activity is defined by monomer–micelle association

Includes: Supplementary data

Articles

Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism

Daisuke Suto, Kazuaki Sato, Yoshihiro Ohba, Tetsuhiko Yoshimura, Junichi Fujii

Biochem J (2005) 392 (2): 399–406.

https://doi.org/10.1042/BJ20050580

Published: 22 November 2005

...), caspase-9 and other cofactors. The issue of whether the redox state of the haem in cyt c affects the initiation of the apoptotic pathway is currently a subject of debate. In a cell-free reconstitution system, we found that only oxidized cyt c was capable of activating the caspase cascade. Oxidized cyt c...

View articletitled, Suppression of the pro-apoptotic function of cytochrome c by singlet oxygen via a haem redox state-independent mechanism

Includes: Supplementary data

Articles

Haem repression of the housekeeping 5-aminolaevulinic acid synthase gene in the hepatoma cell line LMH

Sridevi Kolluri, Timothy J. Sadlon, Brian K. May, Herbert L. Bonkovsky

Biochem J (2005) 392 (1): 173–180.

https://doi.org/10.1042/BJ20050354

Published: 08 November 2005

...Sridevi Kolluri; Timothy J. Sadlon; Brian K. May; Herbert L. Bonkovsky Haem is essential for the health and function of nearly all cells. 5-Aminolaevulinic acid synthase-1 (ALAS-1) catalyses the first and rate-controlling step of haem biosynthesis. ALAS-1 is repressed by haem and is induced...

View articletitled, Haem repression of the housekeeping 5-aminolaevulinic acid synthase gene in the hepatoma cell line LMH

Articles

Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase

Tamara A. DAILEY, John H. WOODRUFF, Harry A. DAILEY

Biochem J (2005) 386 (2): 381–386.

https://doi.org/10.1042/BJ20040570

Published: 22 February 2005

...Tamara A. DAILEY; John H. WOODRUFF; Harry A. DAILEY The initial and the terminal three enzymes of the mammalian haem biosynthetic pathway are nuclear encoded, cytoplasmically synthesized and post-translationally translocated into the mitochondrion. The first enzyme, ALAS (5-aminolaevulinate...

View articletitled, Examination of mitochondrial protein targeting of haem synthetic enzymes: in vivo identification of three functional haem-responsive motifs in 5-aminolaevulinate synthase

Articles

Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast

Emmanuel LESUISSE, Elise R. LYVER, Simon A. B. KNIGHT, Andrew DANCIS

Biochem J (2004) 378 (2): 599–607.

https://doi.org/10.1042/bj20031387

Published: 01 March 2004

... into rho°, consistent with secondary mitochondrial DNA damage from mitochondrial iron accumulation. In fact, in the Δ yhm1 mutant, iron was found to accumulate in mitochondria. The accumulated iron showed decreased availability for haem synthesis, measured in isolated mitochondria using endogenously...

View articletitled, Role of YHM1, encoding a mitochondrial carrier protein, in iron distribution of yeast

Articles

Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

Kiyohiko IGARASHI, Ikuo MOMOHARA, Takeshi NISHINO, Masahiro SAMEJIMA

Biochem J (2002) 365 (2): 521–526.

https://doi.org/10.1042/bj20011809

Published: 15 July 2002

.... Monitoring at the isosbestic point of each prosthetic group indicated that the reductive half-reactions of flavin and haem were biphasic and monophasic respectively. When the observed rates of the flavin and haem reactions were plotted against substrate concentration, the behaviour of the second phase...

View articletitled, Kinetics of inter-domain electron transfer in flavocytochrome cellobiose dehydrogenase from the white-rot fungus Phanerochaete chrysosporium

Articles

Inhibition of neuronal nitric oxide synthase by 6-nitrocatecholamines, putative reaction products of nitric oxide with catecholamines under oxidative stress conditions

Anna PALUMBO, Giuseppe ASTARITA, Marco d'ISCHIA

Biochem J (2001) 356 (1): 105–110.

https://doi.org/10.1042/bj3560105

Published: 08 May 2001

... than 10 μ M) and l -arginine (e.g. 100 μ M). Overall, these results suggest that nitrocatecholamines interfere with nNOS activity by binding to the enzyme in the proximity of the substrate and BH 4 -binding sites near the haem group. 1 To whom correspondence should be addressed (e-mail dischia...

View articletitled, Inhibition of neuronal nitric oxide synthase by 6-nitrocatecholamines, putative reaction products of nitric oxide with catecholamines under oxidative stress conditions

Articles

Physiological oxygen tensions modulate expression of the mdr1b multidrug-resistance gene in primary rat hepatocyte cultures

Karen I. HIRSCH-ERNST, Thomas KIETZMANN, Christina ZIEMANN, Kurt JUNGERMANN, Georg F. KAHL

Biochem J (2000) 350 (2): 443–451.

https://doi.org/10.1042/bj3500443

Published: 23 August 2000

... . Oxygen-dependent modulation of mdr1b mRNA expression was prevented by actinomycin D, indicating transcriptional regulation. Inhibition of haem synthesis by 25 µ M CoCl 2 blocked mdr1b mRNA expression under both oxygen tensions, whereas 80 µ M desferrioxamine abolished modulation by O 2 . Haem (10 µ M...

View articletitled, Physiological oxygen tensions modulate expression of the mdr1b multidrug-resistance gene in primary rat hepatocyte cultures

Articles

Characterization of a flavocytochrome that is induced during the anaerobic respiration of Fe3+ by Shewanella frigidimarina NCIMB400

Paul S. DOBBIN, Julea N. BUTT, Anne K. POWELL, Graeme A. REID, David J. RICHARDSON

Biochem J (1999) 342 (2): 439–448.

https://doi.org/10.1042/bj3420439

Published: 24 August 1999

... terminal electron acceptor, but not in anaerobic cultures of the bacterium with other respiratory substrates. Ifc 3 was purified to homogeneity and revealed by biochemical, spectroscopic and primary structure analyses to contain four low-spin bis-His-ligated c 3 -haems, with midpoint reduction potentials...

View articletitled, Characterization of a flavocytochrome that is induced during the anaerobic respiration of Fe3+ by Shewanella frigidimarina NCIMB400

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