..., Stockholm University, 106 91 Stockholm, Sweden. 21 6 2005 10 10 2005 13 10 2005 13 10 2005 The Biochemical Society, London 2006 fibroblast growth factor (FGF) focal complex heparin heparan sulphate receptor clustering Members of the FGF (fibroblast growth...

...) and GlcNH 2 [(N-unsubstituted) glucosamine] residues of heparan sulphate are potential targets for HOCl. It is shown here that HOCl reacts with each of these residues to generate N-chloro derivatives, and the absolute rate constants for these reactions have been determined. Reaction at GlcNH 2 residues...

...Giancarlo Ghiselli; Amit Agrawal Heparan sulphate (HS) is a ubiquitous constituent of the extracellular matrix that is required for the biological activity of circulating soluble and insoluble extracellular ligands. GLCE ( D -glucuronyl C5-epimerase), an enzyme responsible for the epimerization...

... ). 1 11 2004 1 2 2005 3 3 2005 3 3 2005 The Biochemical Society, London 2005 chemical modification heparan sulphate MS modification degree stable isotope sulphotransferase Nitrous acid digestion was performed at pH 1.5 to cleave exclusively at GlcNS (N-sulphated...

...Ding XU; Vaibhav TIWARI; Guoqing XIA; Christian CLEMENT; Deepak SHUKLA; Jian LIU Heparan sulphate (HS) 3-O-sulphotransferase transfers sulphate to the 3-OH position of the glucosamine residue of HS to form 3-O-sulphated HS. The HS modified by 3-O-sulphotransferase isoform 3 binds to HSV-1 (herpes...

... (heparan sulphate)-binding sequences. To investigate the functional role of these domains, we have generated both wild-type and mutant recombinant anosmin-1 proteins using a Drosophila S2 cell expression system. Here we present the first biochemical evidence demonstrating the high-binding affinity between...

...-lymphocytes by way of interaction with two types of binding sites. The first site corresponds to a signalling receptor; the second site has been identified as heparan sulphate (HS) and appears crucial to induce cell adhesion. Characterization of the HS-binding unit is critical to understand the requirement...

...Anna M. VOGT; Gerhard WINTER; Mats WAHLGREN; Dorothe SPILLMANN HS (heparan sulphate) has hitherto not been found on human red blood cells (RBCs, erythrocytes). However, malarial-parasite ( Plasmodium falciparum )-infected RBCs adhere to uninfected RBCs via HS-like receptors. In the present paper we...

...Chia Lin CHU; J. Ann BUCZEK-THOMAS; Matthew A. NUGENT We investigated how lipid raft association of HSPG (heparan sulphate proteoglycans) modulates FGF-2 (fibroblast growth factor-2/basic fibroblast growth factor) interactions with vascular smooth-muscle cells. When lipid rafts were disrupted...

...-regulated by this inflammatory stimulus. By immunoprecipitation using an anti-syndecan-2 antibody on TNF-α-stimulated HUVEC lysates, inflammation-induced interleukin-8 was found to be an interaction partner of this HS (heparan sulphate) proteoglycan, but not of any other syndecan on these cells...

... The mammalian endoglycosidase heparanase (Hpa1) is primarily responsible for cleaving heparan sulphate proteoglycans (HSPGs) present on the basement membrane of cells and its potential for remodelling the extracellular matrix (ECM) could be important in embryonic development and tumour metastasis. Elevated...

...Emanuel SMEDS; Hiroko HABUCHI; Anh-Tri DO; Eva HJERTSON; Helena GRUNDBERG; Koji KIMATA; Ulf LINDAHL; Marion KUSCHE-GULLBERG Glycosaminoglycan heparan sulphate interacts with a variety of proteins, such as growth factors, cytokines, enzymes and inhibitors and, thus, influences cellular functions...

... transcription and translation yielded a product of 30kDa. Western blotting of chicken embryonic fibroblast cell lysates with species-specific monoclonal antibody mAb 8.1 showed that chicken syndecan-2 is substituted with heparan sulphate, and that the major form of chicken syndecan-2 isolated from chicken...

... parameters and the potency of the oligosaccharides in DNA synthesis assays. These results demonstrate that tetrasaccharides are able to bind FGF-2 and enable FGF-2 to stimulate cell proliferation, which sets important boundary conditions for models of the FGF-2—heparan sulphate—FGF receptor complex. 1...

...Simi ALI; Adrian C. V. PALMER; Sarah J. FRITCHLEY; Yvonne MALEY; John A. KIRBY Chemokines interact with specific G-protein-coupled cell-surface receptors and with glycosaminoglycans (GAGs), such as heparan sulphate. Although chemokines often form multimers in solution, this process may be enhanced...

... mutation-encoded amino acid substitutions, N267K, E514K and F517L, were mapped to structurally defined positions on the GFCC′ β-sheet face of the first and third FnIII domains. Electrostatic maps demonstrated large basic surfaces containing clusters of conserved predicted heparan sulphate-binding residues...

... transformed counterpart (RT101). Expression of perlecan in tumorigenic cells was significantly increased in both mRNA and protein levels. JB6 perlecan was exclusively substituted with heparan sulphate, whereas that of RT101 contained some additional chondroitin sulphate. Detailed structural analysis...

...Michal SAFRAN; Miriam EISENSTEIN; David AVIEZER; Avner YAYON The biological response of cells to fibroblast growth factors (FGFs) depends on heparan sulphate glycosaminoglycans sharing particular structural motifs. Heparin induced FGF dimerization has been suggested to mediate receptor dimerization...

...Karen P. SCHOFIELD; John T. GALLAGHER; Guido DAVID Heparan sulphate proteoglycans (HSPGs) present on the surface of bone marrow stromal cells and in the extracellular matrix (ECM) have important roles in the control of adhesion and growth of haemopoietic stem and progenitor cells. The two main...

... heparanase, which cleaves heparan sulphate (HS), an important structural component of the vascular BL. Previously, tumour-derived heparanase activity (which has been shown to be related to the metastatic potential of murine and human melanoma cell lines) was reported to cleave HS and be inhibited by heparin...

... a molecular mass of 1.1×10 6 Da, and EM demonstrated that it had a globular-protein core structure. The core protein, which showed immunological reactivity with perlecan antibodies, was substituted with approximately seven heparan sulphate (HS) and CS chains of similar size (50-55 kDa), the CS disaccharides...

...Mattias BELTING; Susanne PERSSON; Lars-Åke FRANSSON We have evaluated the possible role of proteoglycans in the uptake of spermine by human lung fibroblasts. Exogenous glycosaminoglycans behaved as competitive inhibitors of spermine uptake, the most efficient being heparan sulphate ( K i = 0.16...