... complex. In contrast with what was known from studies in mammalian phagocytes, in Pichia pastoris we succeeded in producing an active catalytic subunit gp91 phox in absence of its membrane partner. heterologous expression NADPH oxidase Pichia pastoris post-translational modification...

.... chimaeric membrane protein green fluorescent protein heterologous expression proton-translocating inorganic pyrophosphatase Saccharomyces cerevisiae vacuolar-type ATPase © The Authors Journal compilation © 2011 Biochemical Society 2011 10 3 2011 3 5 2011 5 5 2011 26 5...

...) heterologous expression N-terminal signal sequence proton-translocating inorganic pyrophosphatase (H + -PPase) Saccharomyces cerevisiae Heterologous expression may pose important difficulties, especially in the case of multispanning membrane proteins, whose overexpression can be toxic to the host...

... tissues, such as contribution to seed germination and fruit ripening [ 5 ]. heterologous expression thaumatin-like protein (TLP) thaumatin-like xylanase inhibitor (TLXI) slow tight binding wheat ( Triticum aestivum ) xylanase inhibitor At the same time, some plants produce proteins which...

... the Δ gef1 phenotypes were analysed further by DNA subcloning and sequencing. ClC-2 GEF1 heterologous expression Kha1 exchanger Kha1p Saccharomyces cerevisiae The mammalian voltage-gated chloride channel ClC-2 is activated by membrane hyperpolarization, cell swelling and acidic...

... 2004 1 3 2004 1 3 2004 The Biochemical Society, London ©2004 2004 biosynthesis cellular localization endoplasmic reticulum endothelin-converting enzyme-like 1 (ECEL1) heterologous expression monoclonal antibody Abbreviations used: DMEM, Dulbecco's modified Eagle's...

... previously reported in lactic acid-grown cells of native S. cerevisiae strains. Jen1p is the only S. cerevisiae secondary porter characterized so far by heterologous expression in P. pastoris at both the cell and the membrane-vesicle levels. Biochem. J. (2003) 376, 781 787 (Printed in Great Britain) 781...

... and (ii) crystallographic studies of the calcium-channel β 4 subunit. 1 To whom correspondence should be addressed (e-mail [email protected] ). 15 8 2001 5 2 2002 1 3 2002 The Biochemical Society, London ©2002 2002 auxiliary subunit heterologous expression Xenopus...

... Nucleotide Sequence Databases under the accession number AJ133820. 12 7 1999 17 8 1999 15 9 1999 The Biochemical Society, London © 1999 1999 Escherichia coli heterologous expression iron oxygenase Biochem. J. (1999) 344, 397 402 (Printed in Great Britain) 397 2,4...

... reticulum fission yeast heterologous expression protein secretion Biochem. J. (1999) 340, 813 819 (Printed in Great Britain) 813 Interaction of mammalian neprilysin with binding protein and calnexin in Schizosaccharomyces pombe Hugues BEAULIEU, Aram ELAGO$ Z, Philippe CRINE and Luis A. ROKEACH1 De...