... ChaC1 glutathione high-throughput screening molecular dynamics virtual screening ChaC1 is a cytosolic glutathione-degrading enzyme found in higher eukaryotes including humans. It belongs to the γ-glutamylcyclotransferase family, and exclusively degrades reduced glutathione (and not oxidized...

...). Published by Portland Press Limited on behalf of the Biochemical Society 2020 high-throughput screening kinases Plasmodium falciparum Plasmodium falciparum is the most prevalent malaria parasite which accounted for a majority of the total estimated malaria cases in WHO African Region...

... Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) . autophagy chemical biology high-throughput screening kinases Macroautophagy (referred to as autophagy here for simplicity) is a lysosome-dependent degradative pathway...

... 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 autophagy biotechnology high-throughput screening mitochondrial function Basal respiration was recorded for 30 min, at ∼5 min intervals until system stabilization. Glucose was injected...

[email protected] ) © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 cathepsin K collagen exosite inhibitor high-throughput screening osteoporosis protease inhibitor In this study, we employed a high-throughput fluorescence polarization (FP...

... of the bacterial cell wall. Inhibition results in a loss of cell viability. GlmU is therefore considered a potential target for novel antibacterial agents. A HTS (high-throughput screen) identified a series of aminoquinazolines with submicromolar potency against the uridyltransferase reaction. Biochemical...

... correspondence should be addressed (email [email protected] ). 21 10 2009 8 5 2010 27 5 2010 27 5 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 anti-retroviral therapy high-diversity chemical library high-throughput screening HIV-1 kinetics non...

... are difficult to perform, particularly in HTS (high-throughput screening) mode. In the present study, we stably co-expressed c-Myc and eGFP [enhanced GFP (green fluorescent protein)] dual-tagged recombinant GLUT4 with recombinant IRS1 (insulin receptor substrate 1) in HEK-293 cells (human embryonic kidney cells...

...., in this issue of the Biochemical Journal . 1 email [email protected] 19 3 2008 4 4 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 CXCR2 chemokine drug design G-protein-coupled receptor dimerization high-throughput screening imaging-based...

... successfully miniaturized to a sufficiently low volume to allow it to be utilized in a high-throughput screen (384-well format; 20 μl reaction volume), enabling the assay to be used in screening campaigns against libraries of compounds to discover leads for further drug development. 1 To whom...

... recycling, 5,5«-dithio-bis-(2-nitrobenzoic acid), high-throughput screening. This is a stopped assay that requires additional chemical ma- nipulation and, despite the increase in assay sensitivity, un- reasonable quantities of T[S] # are still required for the screening of large compound libraries. Use...

...Heike ROSS; Christopher G. ARMSTRONG; Philip COHEN The generation of drugs that modulate the activities of particular protein kinases has become a prime focus of the pharmaceutical and biotechnology industry. Consequently, improved methods for the development of high-throughput screening formats...

.... These assays, which can be used directly on tissue homogenates, are the most sensitive and selective described to date for ECE, and are easily automated for a high-throughput screening of inhibitors. 1 To whom correspondence should be addressed (e-mail [email protected] ). 2 1 2001...

... of signal transduction. 1 To whom correspondence should be addressed (e-mail [email protected] ). 11 4 2000 2 6 2000 26 6 2000 The Biochemical Society, London © 2000 2000 CREB granulocyte high-throughput screening macrophage prostaglandin Biochem. J...