...) immunoprecipitation protein N-terminal acetylation N α -terminal protein acetylation is a widespread modification in eukaryotes. Despite this, its biological function and importance are largely unknown [ 1 ]. N α -terminal acetylation is believed to be a co-translational process, where nascent polypeptides...

... to neurotransmitter receptors describes molecular mechanisms of synaptic signal transduction. To identify interacting proteins of GABA C (where GABA is γ-aminobutyric acid) receptors in the retina, we used antibodies specific for GABA C receptor ρ1–3 subunits. Analysis of immunoprecipitated proteins by MALDI–TOF MS...

... with presenilins. 1 To whom correspondence should be addressed (email [email protected] ). 23 3 2006 28 6 2006 3 7 2006 3 7 2006 The Biochemical Society, London 2006 dimerization immunoprecipitation oligomerization presenilin ubiquilin yeast two-hydrid assay...

... and immunoprecipitation analyses indicated that the protein is subunit IV of the cytochrome c oxidase complex (COIV). In vitro phosphorylation of COIV was attenuated by PKC pseudosubstrate peptides. Introduction of an PKCε-selective translocation inhibitor [Johnson, Gray, Chen and Mochly-Rosen (1996) J. Biol. Chem. 271...

... ventricular myocardium. Endogenous phosphorylation of RyR2 was verified using phosphorylation site-specific antibodies. Co-immunoprecipitation studies established that RyR2 was physically associated with CaMKIIδ. Quantitative assessment of RyR2 protein was performed by [ 3 H]ryanodine binding to RyR2...

...–UbcH7 thioester, but not free UbcH7, which enabled the measurement of the respective cellular levels separately. The immunoprecipitable ubiquitin–UbcH7 thioester was found only when the cells were treated with heat-shock. These results suggest the usefulness of the immunoaffinity techniques...

... are expressed in the central nervous system, but their functions and mechanisms of coupling are still poorly understood. We show here that complexes immunoprecipitated from solubilized rat brain membranes with antibodies against DHPR of the Ca v 1.2 or Ca v 1.3 subtypes contain RyR. Only type-1 RyR is co...

... for cofilin, an actin-binding protein. Cofilin was co-immunoprecipitated with the α subunit of Na,K-ATPase from extracts of COS-7 cells transiently transfected with haemagglutinin-epitope-tagged cofilin cDNA as well as from yeast extracts. By means of deletion analysis we showed that the segment of cofilin...

...- μ to control levels in cells devoid of OP 3 . This characterizes β-CNA as an inverse OP 3 agonist. Immunoprecipitation of solubilized G-proteins with G i3 α antisera demonstrated that basal GTP[ 35 S] binding to G i3 α was also substantially elevated in HEK- μ membranes over the control, whereas G...

..., exposure of collagen type II-cultured chondrocytes to IGF-I leads to co-immunoprecipitation of Shc protein with the IGF-IR and with β1, α1 and α5 integrins, but not with α3 integrin. Shc then associates with growth factor receptor-bound protein 2 (Grb2), an adaptor protein and extracellular signal...

... untreated wild-type mice; expression increased when mice were treated with bacterial lipopolysaccharide (LPS). No NOS II was found in any tissue of untreated or LPS-treated NOS II-deficient mice. Immunoprecipitation experiments were performed with homogenates of gastrocnemius muscle from untreated or LPS...