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Articles

Non-cross-linking advanced glycation end products affect prohormone processing

Sebastian Brings, Walter Mier, Barbro Beijer, Elisabeth Kliemank, Stephan Herzig, Julia Szendroedi, Peter P. Nawroth, Thomas Fleming

Biochem J (2024) 481 (1): 33–44.

https://doi.org/10.1042/BCJ20230321

Published: 04 January 2024

... to generate unmodified as well as AGE-modified protease substrates. Activity of proteases towards these substrates was quantified. Second, we tested the effect of AGE-modified proinsulin on the processing to insulin. Proteases showed the expected activity towards the unmodified peptide substrates containing...

View article titled, Non-cross-linking advanced glycation end products affect prohormone processing

Includes: Supplementary data

Articles

Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

In Collection

Awards

Guy A. Rutter, Vaibhav Sidarala, Brett A. Kaufman, Scott A. Soleimanpour

Biochem J (2023) 480 (11): 773–789.

https://doi.org/10.1042/BCJ20230167

Published: 07 June 2023

...Guy A. Rutter; Vaibhav Sidarala; Brett A. Kaufman; Scott A. Soleimanpour Glucose-regulated insulin secretion becomes defective in all forms of diabetes. The signaling mechanisms through which the sugar acts on the ensemble of beta cells within the islet remain a vigorous area of research after more...

View article titled, Mitochondrial metabolism and dynamics in pancreatic beta cell glucose sensing

Articles

GLUT4 On the move

Daniel J. Fazakerley, Francoise Koumanov, Geoffrey D. Holman

Biochem J (2022) 479 (3): 445–462.

https://doi.org/10.1042/BCJ20210073

Published: 11 February 2022

...Daniel J. Fazakerley; Francoise Koumanov; Geoffrey D. Holman Insulin rapidly stimulates GLUT4 translocation and glucose transport in fat and muscle cells. Signals from the occupied insulin receptor are translated into downstream signalling changes in serine/threonine kinases within timescales...

View article titled, GLUT4 On the move

Includes: Supplementary data

Articles

Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

In Collection

Celebrating 100 years of insulin research

Sara M. Frangos, David J. Bishop, Graham P. Holloway

Biochem J (2021) 478 (21): 3809–3826.

https://doi.org/10.1042/BCJ20210145

Published: 09 November 2021

...Sara M. Frangos; David J. Bishop; Graham P. Holloway While the etiology of type 2 diabetes is multifaceted, the induction of insulin resistance in skeletal muscle is a key phenomenon, and impairments in insulin signaling in this tissue directly contribute to hyperglycemia. Despite the lack...

View article titled, Revisiting the contribution of mitochondrial biology to the pathophysiology of skeletal muscle insulin resistance

Articles

Interactions between insulin and exercise

In Collection

Celebrating 100 years of insulin research

Erik A. Richter, Lykke Sylow, Mark Hargreaves

Biochem J (2021) 478 (21): 3827–3846.

https://doi.org/10.1042/BCJ20210185

Published: 09 November 2021

...Erik A. Richter; Lykke Sylow; Mark Hargreaves The interaction between insulin and exercise is an example of balancing and modifying the effects of two opposing metabolic regulatory forces under varying conditions. While insulin is secreted after food intake and is the primary hormone increasing...

View article titled, Interactions between insulin and exercise

Articles

A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner

Franziska Kopietz, Yazeed Alshuweishi, Silvia Bijland, Fatmah Alghamdi, Eva Degerman, Kei Sakamoto, Ian P. Salt, Olga Göransson

Biochem J (2021) 478 (3): 633–646.

https://doi.org/10.1042/BCJ20200659

Published: 12 February 2021

... of the allosteric AMPK activators A-769662 and 991 on glucose uptake in adipocytes. For this purpose, primary rat or human adipocytes, and cultured 3T3-L1 adipocytes, were treated with either of the allosteric activators, or AICAR, and basal and insulin-stimulated glucose uptake was assessed. Additionally...

View article titled, A-769662 inhibits adipocyte glucose uptake in an AMPK-independent manner

Includes: Supplementary data

Articles

Effects of PKB/Akt inhibitors on insulin-stimulated lipogenesis and phosphorylation state of lipogenic enzymes in white adipose tissue

Nusrat Hussain, Sheng-Ju Chuang, Manuel Johanns, Didier Vertommen, Gregory R. Steinberg, Bruce E. Kemp, Mark H. Rider

Biochem J (2020) 477 (8): 1373–1389.

https://doi.org/10.1042/BCJ20190788

Published: 23 April 2020

...Nusrat Hussain; Sheng-Ju Chuang; Manuel Johanns; Didier Vertommen; Gregory R. Steinberg; Bruce E. Kemp; Mark H. Rider We investigated acute effects of two allosteric protein kinase B (PKB) inhibitors, MK-2206 and Akti-1/2, on insulin-stimulated lipogenesis in rat epididymal adipocytes incubated...

View article titled, Effects of PKB/Akt inhibitors on insulin-stimulated lipogenesis and phosphorylation state of lipogenic enzymes in white adipose tissue

Includes: Supplementary data

Articles

Scaling it down: new in vitro tools to get the balance right

Emma K. Biggs, Fiona M. Gribble, Frank Reimann

Biochem J (2017) 474 (1): 47–50.

https://doi.org/10.1042/BCJ20160874

Published: 22 December 2016

... © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 CRISPR incretin insulin Cas9 CRISPR associated protein 9 GIP glucose-dependent insulinotropic polypeptide GLP-1 glucagon-like peptide-1 indels insertions...

View article titled, Scaling it down: new in vitro tools to get the balance right

Articles

Two insulin-like peptides differentially regulate malaria parasite infection in the mosquito through effects on intermediary metabolism

Jose E. Pietri, Nazzy Pakpour, Eleonora Napoli, Gyu Song, Eduardo Pietri, Rashaun Potts, Kong W. Cheung, Gregory Walker, Michael A. Riehle, Hannah Starcevich, Cecilia Giulivi, Edwin E. Lewis, Shirley Luckhart

Biochem J (2016) 473 (20): 3487–3503.

https://doi.org/10.1042/BCJ20160271

Published: 11 October 2016

...Jose E. Pietri; Nazzy Pakpour; Eleonora Napoli; Gyu Song; Eduardo Pietri; Rashaun Potts; Kong W. Cheung; Gregory Walker; Michael A. Riehle; Hannah Starcevich; Cecilia Giulivi; Edwin E. Lewis; Shirley Luckhart Insulin-like peptides (ILPs) play important roles in growth and metabolic homeostasis...

View article titled, Two insulin-like peptides differentially regulate malaria parasite infection in the mosquito through effects on intermediary metabolism

Includes: Supplementary data

Articles

Rab14 limits the sorting of Glut4 from endosomes into insulin-sensitive regulated secretory compartments in adipocytes

Paul Duffield Brewer, Estifanos N. Habtemichael, Irina Romenskaia, Adelle C.F. Coster, Cynthia Corley Mastick

Biochem J (2016) 473 (10): 1315–1327.

https://doi.org/10.1042/BCJ20160020

Published: 11 May 2016

...Paul Duffield Brewer; Estifanos N. Habtemichael; Irina Romenskaia; Adelle C.F. Coster; Cynthia Corley Mastick Insulin increases glucose uptake by increasing the rate of exocytosis of the facilitative glucose transporter isoform 4 (Glut4) relative to its endocytosis. Insulin also releases Glut4 from...

View article titled, Rab14 limits the sorting of Glut4 from endosomes into insulin-sensitive regulated secretory compartments in adipocytes

Includes: Supplementary data

Articles

Protein kinase Cζ exhibits constitutive phosphorylation and phosphatidylinositol-3,4,5-triphosphate-independent regulation

Irene S. Tobias, Manuel Kaulich, Peter K. Kim, Nitya Simon, Estela Jacinto, Steven F. Dowdy, Charles C. King, Alexandra C. Newton

Biochem J (2016) 473 (4): 509–523.

https://doi.org/10.1042/BJ20151013

Published: 09 February 2016

...Irene S. Tobias; Manuel Kaulich; Peter K. Kim; Nitya Simon; Estela Jacinto; Steven F. Dowdy; Charles C. King; Alexandra C. Newton Atypical protein kinase C (aPKC) isoenzymes are key modulators of insulin signalling, and their dysfunction correlates with insulin-resistant states in both mice...

View article titled, Protein kinase Cζ exhibits constitutive phosphorylation and phosphatidylinositol-3,4,5-triphosphate-independent regulation

Articles

Adiponectin is released via a unique regulated exocytosis pathway from a pre-formed vesicle pool on insulin stimulation

Chun-Yan Lim, Wanjin Hong, Weiping Han

Biochem J (2015) 471 (3): 381–389.

https://doi.org/10.1042/BJ20150301

Published: 16 October 2015

...Chun-Yan Lim; Wanjin Hong; Weiping Han Adiponectin, a hormone secreted from adipocytes and released at a high rate into the circulation, plays a pivotal role in maintaining insulin sensitivity at the whole-body level. Despite the importance of this adipokine in metabolic homoeostasis, the mechanism...

View article titled, Adiponectin is released via a unique regulated exocytosis pathway from a pre-formed vesicle pool on insulin stimulation

Includes: Multimedia, Supplementary data

Articles

GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling

Clare Stretton, Thorsten M. Hoffmann, Michael J. Munson, Alan Prescott, Peter M. Taylor, Ian G. Ganley, Harinder S. Hundal

Biochem J (2015) 470 (2): 207–221.

https://doi.org/10.1042/BJ20150404

Published: 20 August 2015

...) signalling in response to amino acid availability. GSK3 inhibition is associated with reduced mTORC1 signalling that impacts negatively on cell growth, protein synthesis and promotes cellular autophagy. amino acid autophagy growth insulin L-type (leucine) amino acid transporter 1 (LAT1) leucine...

View article titled, GSK3-mediated raptor phosphorylation supports amino-acid-dependent mTORC1-directed signalling

Includes: Supplementary data

Articles

Role for RalA downstream of Rac1 in skeletal muscle insulin signalling

Nobuyuki Takenaka, Yukio Sumi, Keiko Matsuda, Junko Fujita, Tetsuya Hosooka, Tetsuya Noguchi, Atsu Aiba, Takaya Satoh

Biochem J (2015) 469 (3): 445–454.

https://doi.org/10.1042/BJ20150218

Published: 23 July 2015

...Nobuyuki Takenaka; Yukio Sumi; Keiko Matsuda; Junko Fujita; Tetsuya Hosooka; Tetsuya Noguchi; Atsu Aiba; Takaya Satoh Insulin-stimulated glucose uptake in skeletal muscle is mediated by the translocation of the glucose transporter GLUT4 from intracellular storage sites to the plasma membrane...

View article titled, Role for RalA downstream of Rac1 in skeletal muscle insulin signalling

Includes: Supplementary data

Articles

Metabolomic profiling in liver of adiponectin-knockout mice uncovers lysophospholipid metabolism as an important target of adiponectin action

Ying Liu, Sanjana Sen, Sivaporn Wannaiampikul, Rengasamy Palanivel, Ruby L. C. Hoo, Ruth Isserlin, Gary D. Bader, Rungsunn Tungtrongchitr, Yves Deshaies, Aimin Xu, Gary Sweeney

Biochem J (2015) 469 (1): 71–82.

https://doi.org/10.1042/BJ20141455

Published: 19 June 2015

...Ying Liu; Sanjana Sen; Sivaporn Wannaiampikul; Rengasamy Palanivel; Ruby L. C. Hoo; Ruth Isserlin; Gary D. Bader; Rungsunn Tungtrongchitr; Yves Deshaies; Aimin Xu; Gary Sweeney Adiponectin mediates anti-diabetic effects via increasing hepatic insulin sensitivity and direct metabolic effects...

View article titled, Metabolomic profiling in liver of adiponectin-knockout mice uncovers lysophospholipid metabolism as an important target of adiponectin action

Includes: Supplementary data

Articles

Inhibition of the malate–aspartate shuttle in mouse pancreatic islets abolishes glucagon secretion without affecting insulin secretion

Jelena A. Stamenkovic, Lotta E. Andersson, Alice E. Adriaenssens, Annika Bagge, Vladimir V. Sharoyko, Fiona Gribble, Frank Reimann, Claes B. Wollheim, Hindrik Mulder, Peter Spégel

Biochem J (2015) 468 (1): 49–63.

https://doi.org/10.1042/BJ20140697

Published: 05 May 2015

...Jelena A. Stamenkovic; Lotta E. Andersson; Alice E. Adriaenssens; Annika Bagge; Vladimir V. Sharoyko; Fiona Gribble; Frank Reimann; Claes B. Wollheim; Hindrik Mulder; Peter Spégel Altered secretion of insulin as well as glucagon has been implicated in the pathogenesis of Type 2 diabetes (T2D...

View article titled, Inhibition of the malate–aspartate shuttle in mouse pancreatic islets abolishes glucagon secretion without affecting insulin secretion

Includes: Supplementary data

Articles

The insulinotrophic effect of insulin-like peptide 5 in vitro and in vivo

Xiao Luo, Ting Li, Yue Zhu, Yunbin Dai, Jianwei Zhao, Zhan-Yun Guo, Ming-Wei Wang

Biochem J (2015) 466 (3): 467–473.

https://doi.org/10.1042/BJ20141113

Published: 06 March 2015

...Xiao Luo; Ting Li; Yue Zhu; Yunbin Dai; Jianwei Zhao; Zhan-Yun Guo; Ming-Wei Wang Insulin-like peptide 5 (INSL5), a member of the insulin/relaxin superfamily, can activate the G-protein-coupled receptor relaxin/insulin-like family peptide receptor 4 (RXFP4), but its precise biological functions...

View article titled, The insulinotrophic effect of insulin-like peptide 5 in vitro and in vivo

Articles

Pancreatic β-cell identity, glucose sensing and the control of insulin secretion

Guy A. Rutter, Timothy J. Pullen, David J. Hodson, Aida Martinez-Sanchez

Biochem J (2015) 466 (2): 203–218.

https://doi.org/10.1042/BJ20141384

Published: 20 February 2015

...Guy A. Rutter; Timothy J. Pullen; David J. Hodson; Aida Martinez-Sanchez Insulin release from pancreatic β-cells is required to maintain normal glucose homoeostasis in man and many other animals. Defective insulin secretion underlies all forms of diabetes mellitus, a disease currently reaching...

View article titled, Pancreatic β-cell identity, glucose sensing and the control of insulin secretion

Articles

Insulin-stimulated leptin secretion requires calcium and PI3K/Akt activation

Yue Wang, Yusuf Ali, Chun-Yan Lim, Wanjin Hong, Zhiping P. Pang, Weiping Han

Biochem J (2014) 458 (3): 491–498.

https://doi.org/10.1042/BJ20131176

Published: 28 February 2014

... leptin storage and secretion regulation in 3T3-L1 and primary adipocytes. Leptin is stored in membrane-bound vesicles that are localized predominantly in the ER (endoplasmic reticulum) and close to the plasma membrane of both 3T3-L1 and primary adipocytes. Insulin increases leptin secretion as early...

View article titled, Insulin-stimulated leptin secretion requires calcium and PI3K/Akt activation

Includes: Supplementary data

Articles

Down-regulation of cyclin G2 by insulin, IGF-I (insulin-like growth factor 1) and X10 (AspB10 insulin): role in mitogenesis

Angela M. Svendsen, Sofia B. Winge, Maike Zimmermann, Anne B. Lindvig, Caroline B. Warzecha, Waseem Sajid, Mary C. Horne, Pierre De Meyts

Biochem J (2014) 457 (1): 69–77.

https://doi.org/10.1042/BJ20130490

Published: 10 December 2013

...Angela M. Svendsen; Sofia B. Winge; Maike Zimmermann; Anne B. Lindvig; Caroline B. Warzecha; Waseem Sajid; Mary C. Horne; Pierre De Meyts The mechanisms whereby insulin analogues may cause enhanced mitogenicity through activation of either the IR (insulin receptor) or the IGF-IR (insulin-like...

View article titled, Down-regulation of cyclin G2 by insulin, IGF-I (insulin-like growth factor 1) and X10 (AspB10 insulin): role in mitogenesis

Includes: Supplementary data

Articles

FGT-1 is the major glucose transporter in C. elegans and is central to aging pathways

Ying Feng, Barnabas G. Williams, Françoise Koumanov, Adrian J. Wolstenholme, Geoffrey D. Holman

Biochem J (2013) 456 (2): 219–229.

https://doi.org/10.1042/BJ20131101

Published: 08 November 2013

...Ying Feng; Barnabas G. Williams; Françoise Koumanov; Adrian J. Wolstenholme; Geoffrey D. Holman Caenorhabditis elegans is widely used as a model for investigation of the relationships between aging, nutrient restriction and signalling via the DAF-2 (abnormal dauer formation 2) receptor for insulin...

View article titled, FGT-1 is the major glucose transporter in C. elegans and is central to aging pathways

Includes: Supplementary data

Articles

PIKfyve: a new fish in the growing pool of AMPK substrates

James S. V. Lally, Gregory R. Steinberg

Biochem J (2013) 455 (2): e1–e3.

https://doi.org/10.1042/BJ20131138

Published: 27 September 2013

...James S. V. Lally; Gregory R. Steinberg Skeletal muscle is critical for whole-body glucose homoeostasis. Insulin and muscle contractions induced by exercise can increase glucose uptake through distinct intracellular signalling pathways involving PKB (protein kinase B)/Akt and AMPK (AMP-activated...

View article titled, PIKfyve: a new fish in the growing pool of AMPK substrates

Articles

Phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) is an AMPK target participating in contraction-stimulated glucose uptake in skeletal muscle

Yang Liu, Yu-Chiang Lai, Elaine V. Hill, Donatienne Tyteca, Sarah Carpentier, Ada Ingvaldsen, Didier Vertommen, Louise Lantier, Marc Foretz, Franck Dequiedt, Pierre J. Courtoy, Christophe Erneux, Benoît Viollet, Peter R. Shepherd, Jeremy M. Tavaré, Jørgen Jensen, Mark H. Rider

Biochem J (2013) 455 (2): 195–206.

https://doi.org/10.1042/BJ20130644

Published: 27 September 2013

...-containing phosphatidylinositol 3-phosphate 5-kinase), the lipid kinase that phosphorylates PtdIns3 P to PtdIns(3,5) P 2 , has been implicated in insulin-stimulated glucose uptake. We investigated whether PIKfyve could also be involved in contraction/AMPK (AMP-activated protein kinase)-stimulated glucose...

View article titled, Phosphatidylinositol 3-phosphate 5-kinase (PIKfyve) is an AMPK target participating in contraction-stimulated glucose uptake in skeletal muscle

Includes: Supplementary data

Articles

Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Tetsuya Kitaguchi, Manami Oya, Yoshiko Wada, Takashi Tsuboi, Atsushi Miyawaki

Biochem J (2013) 450 (2): 365–373.

https://doi.org/10.1042/BJ20121022

Published: 15 February 2013

...Tetsuya Kitaguchi; Manami Oya; Yoshiko Wada; Takashi Tsuboi; Atsushi Miyawaki Intracellular cAMP and Ca 2+ are important second messengers that regulate insulin secretion in pancreatic β-cells; however, the molecular mechanism underlying their mutual interaction for exocytosis is not fully...

View article titled, Extracellular calcium influx activates adenylate cyclase 1 and potentiates insulin secretion in MIN6 cells

Includes: Supplementary data

Articles

The AMPK-related kinase SIK2 is regulated by cAMP via phosphorylation at Ser 358 in adipocytes

Emma Henriksson, Helena A. Jones, Kashyap Patel, Mark Peggie, Nicholas Morrice, Kei Sakamoto, Olga Göransson

Biochem J (2012) 444 (3): 503–514.

https://doi.org/10.1042/BJ20111932

Published: 29 May 2012

... in adipocytes in response to cellular stimuli with relevance for adipocyte function and/or AMPK signalling. None of the treatments, including insulin, cAMP inducers or AICAR (5-amino-4-imidazolecarboxamide riboside), affected SIK2 activity towards peptide or protein substrates in vitro . However, stimulation...

View article titled, The AMPK-related kinase SIK2 is regulated by cAMP via phosphorylation at Ser 358 in adipocytes

Articles

The anti-neurodegenerative agent clioquinol regulates the transcription factor FOXO1a

Amy R. Cameron, Katherine Wallace, Lisa Logie, Alan R. Prescott, Terry G. Unterman, Jean Harthill, Graham Rena

Biochem J (2012) 443 (1): 57–64.

https://doi.org/10.1042/BJ20112124

Published: 14 March 2012

... underlying these diseases, with the scope to identify common cellular targets for therapy. In the present study we have examined the insulin-like signalling properties of an experimental AD 8-hydroxyquinoline drug known as CQ (clioquinol). The IIS [insulin/IGF-1 (insulin-like growth factor-1) signalling...

View article titled, The anti-neurodegenerative agent clioquinol regulates the transcription factor FOXO1a

Articles

Reprogramming of pancreatic exocrine cells towards a beta (β) cell character using Pdx1 , Ngn3 and MafA

Ersin Akinci, Anannya Banga, Lucas V. Greder, James R. Dutton, Jonathan M. W. Slack

Biochem J (2012) 442 (3): 539–550.

https://doi.org/10.1042/BJ20111678

Published: 24 February 2012

...). When introduced into AR42j-B13 cells, Ad-PNM caused a rapid change to a flattened morphology and a cessation of cell division. The expression of exocrine markers is suppressed. Both insulin genes are up-regulated as well as a number of transcription factors normally characteristic of beta cells...

View article titled, Reprogramming of pancreatic exocrine cells towards a beta (β) cell character using Pdx1 , Ngn3 and MafA

Includes: Supplementary data

Articles

IκB kinase β (IKKβ) does not mediate feedback inhibition of the insulin signalling cascade

Graeme I. Lancaster, Beata Skiba, Christine Yang, Hayley T. Nicholls, Katherine G. Langley, M. H. Stanley Chan, Clinton R. Bruce, Gordon W. Rewcastle, Peter R. Shepherd, Michael Karin, Mark A. Febbraio

Biochem J (2012) 442 (3): 723–732.

https://doi.org/10.1042/BJ20112037

Published: 24 February 2012

... β] plays a role in feedback inhibition of the insulin signalling cascade. Insulin induces the phosphorylation of IKKβ, in vitro and in vivo , and this effect is dependent on intact signalling via PI3K (phosphoinositide 3-kinase), but not PKB (protein kinase B). To test the hypothesis that insulin...

View article titled, IκB kinase β (IKKβ) does not mediate feedback inhibition of the insulin signalling cascade

Includes: Supplementary data

Articles

ALAS1 gene expression is down-regulated by Akt-mediated phosphorylation and nuclear exclusion of FOXO1 by vanadate in diabetic mice

Leda M. Oliveri, Carlos Davio, Alcira M. del C. Batlle, Esther N. Gerez

Biochem J (2012) 442 (2): 303–310.

https://doi.org/10.1042/BJ20111005

Published: 13 February 2012

...), the first and rate-limiting enzyme of the haem pathway. Acute porphyria attacks are usually treated by the administration of glucose; its effect is apparently related to its ability to inhibit ALAS1 by modulating insulin plasma levels. It has been shown that insulin blunts hepatocyte ALAS1 induction...

View article titled, ALAS1 gene expression is down-regulated by Akt-mediated phosphorylation and nuclear exclusion of FOXO1 by vanadate in diabetic mice

Articles

Epidermal growth factor induces tumour marker AKR1B10 expression through activator protein-1 signalling in hepatocellular carcinoma cells

Ziwen Liu, Ruilan Yan, Ahmed Al-Salman, Yi Shen, Yiwen Bu, Jun Ma, Di-Xian Luo, Chenfei Huang, Yuyang Jiang, Andrew Wilber, Yin-Yuan Mo, Mei Chris Huang, Yupei Zhao, Deliang Cao

Biochem J (2012) 442 (2): 273–282.

https://doi.org/10.1042/BJ20111322

Published: 13 February 2012

... growth factor) and insulin through the AP-1 (activator protein-1) signalling pathway. In human HCC (hepatocellular carcinoma) cells (HepG2 and Hep3B), EGF (50 ng/ml) and insulin (10 nM) stimulated endogenous AKR1B10 expression and promoter activity. In the AKR1B10 promoter, a putative AP-1 element...

View article titled, Epidermal growth factor induces tumour marker AKR1B10 expression through activator protein-1 signalling in hepatocellular carcinoma cells

Articles

Effects of acutely inhibiting PI3K isoforms and mTOR on regulation of glucose metabolism in vivo

Greg C. Smith, Wee Kiat Ong, Gordon W. Rewcastle, Jackie D. Kendall, Weiping Han, Peter R. Shepherd

Biochem J (2012) 442 (1): 161–169.

https://doi.org/10.1042/BJ20111913

Published: 27 January 2012

... and appetite. However, in these models the PI3K activity is chronically reduced. Therefore we analysed the effects of acutely inhibiting PI3K isoforms alone, or PI3K and mTOR, on glucose metabolism and food intake. In the present study impairments in glucose tolerance, insulin tolerance and increased hepatic...

View article titled, Effects of acutely inhibiting PI3K isoforms and mTOR on regulation of glucose metabolism in vivo

Includes: Supplementary data

Articles

Insight into the molecular basis for the kinetic differences between the two insulin receptor isoforms

Louise Knudsen, Pierre De Meyts, Vladislav V. Kiselyov

Biochem J (2011) 440 (3): 397–403.

https://doi.org/10.1042/BJ20110550

Published: 28 November 2011

...Louise Knudsen; Pierre De Meyts; Vladislav V. Kiselyov More than 20 years after the description of the two IR (insulin receptor) isoforms, designated IR-A (lacking exon 11) and IR-B (with exon 11), nearly every functional aspect of the alternative splicing both in vitro and in vivo remains...

View article titled, Insight into the molecular basis for the kinetic differences between the two insulin receptor isoforms

Articles

Counter-modulation of fatty acid-induced pro-inflammatory nuclear factor κB signalling in rat skeletal muscle cells by AMP-activated protein kinase

Charlotte J. Green, Katherine Macrae, Sarah Fogarty, D. Grahame Hardie, Kei Sakamoto, Harinder S. Hundal

Biochem J (2011) 435 (2): 463–474.

https://doi.org/10.1042/BJ20101517

Published: 29 March 2011

...Charlotte J. Green; Katherine Macrae; Sarah Fogarty; D. Grahame Hardie; Kei Sakamoto; Harinder S. Hundal Sustained over-supply of saturated non-esterified ‘free’ fatty acids has been shown to promote skeletal muscle insulin resistance, which may be driven, in part, by an increase in inflammatory...

View article titled, Counter-modulation of fatty acid-induced pro-inflammatory nuclear factor κB signalling in rat skeletal muscle cells by AMP-activated protein kinase

Includes: Supplementary data

Articles

Next-generation Akt inhibitors provide greater specificity: effects on glucose metabolism in adipocytes

Shixiong Tan, Yvonne Ng, David E. James

Biochem J (2011) 435 (2): 539–544.

https://doi.org/10.1042/BJ20110040

Published: 29 March 2011

... for processes including glucose metabolism. Therefore a potential complication of such anticancer drugs is insulin resistance and/or diabetes. In the process of characterizing the metabolic effects of early-phase Akt inhibitors, we discovered an off-target inhibitory effect on mammalian facilitative glucose...

View article titled, Next-generation Akt inhibitors provide greater specificity: effects on glucose metabolism in adipocytes

Articles

Metabolomic analyses reveal profound differences in glycolytic and tricarboxylic acid cycle metabolism in glucose-responsive and -unresponsive clonal β-cell lines

Peter Spégel, Siri Malmgren, Vladimir V. Sharoyko, Philip Newsholme, Thomas Koeck, Hindrik Mulder

Biochem J (2011) 435 (1): 277–284.

https://doi.org/10.1042/BJ20100655

Published: 15 March 2011

...Peter Spégel; Siri Malmgren; Vladimir V. Sharoyko; Philip Newsholme; Thomas Koeck; Hindrik Mulder Insulin secretion from pancreatic β-cells is controlled by complex metabolic and energetic changes provoked by exposure to metabolic fuels. Perturbations in these processes lead to impaired insulin...

View article titled, Metabolomic analyses reveal profound differences in glycolytic and tricarboxylic acid cycle metabolism in glucose-responsive and -unresponsive clonal β-cell lines

Includes: Supplementary data

Articles

Translocation of the Na + /H + exchanger 1 (NHE1) in cardiomyocyte responses to insulin and energy-status signalling

Scott P. Lawrence, Geoffrey D. Holman, Françoise Koumanov

Biochem J (2010) 432 (3): 515–525.

https://doi.org/10.1042/BJ20100717

Published: 25 November 2010

... in the activation process. We have determined the distribution of NHE1 by means of immunofluorescence microscopy and cell-surface biotinylation. We have discovered changes in the distribution of NHE1 that occur when cardiomyocytes are stimulated with insulin that are PI3K (phosphoinositide 3-kinase)-dependent...

View article titled, Translocation of the Na + /H + exchanger 1 (NHE1) in cardiomyocyte responses to insulin and energy-status signalling

Includes: Supplementary data

Articles

Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells

Kyota Aoyagi, Mica Ohara-Imaizumi, Chiyono Nishiwaki, Yoko Nakamichi, Shinya Nagamatsu

Biochem J (2010) 432 (2): 375–386.

https://doi.org/10.1042/BJ20100864

Published: 12 November 2010

...Kyota Aoyagi; Mica Ohara-Imaizumi; Chiyono Nishiwaki; Yoko Nakamichi; Shinya Nagamatsu Functional insulin receptor and its downstream effector PI3K (phosphoinositide 3-kinase) have been identified in pancreatic β-cells, but their involvement in the regulation of insulin secretion from β-cells...

View article titled, Insulin/phosphoinositide 3-kinase pathway accelerates the glucose-induced first-phase insulin secretion through TrpV2 recruitment in pancreatic β-cells

Articles

Akt inhibitors reduce glucose uptake independently of their effects on Akt

Shi-Xiong Tan, Yvonne Ng, David E. James

Biochem J (2010) 432 (1): 191–198.

https://doi.org/10.1042/BJ20100750

Published: 25 October 2010

..., Akt also plays an essential role in other physiological processes, such as the insulin-regulated transport of glucose into muscle and fat cells. This process, which is essential for whole-body glucose homoeostasis in mammals, is thought to be mediated via Akt-dependent movement of GLUT4 glucose...

View article titled, Akt inhibitors reduce glucose uptake independently of their effects on Akt

Includes: Supplementary data

Articles

Glucose promotion of GABA metabolism contributes to the stimulation of insulin secretion in β-cells

Javier Pizarro-Delgado, Matthias Braun, Inés Hernández-Fisac, Rafael Martín-Del-Río, Jorge Tamarit-Rodriguez

Biochem J (2010) 431 (3): 381–390.

https://doi.org/10.1042/BJ20100714

Published: 11 October 2010

...Javier Pizarro-Delgado; Matthias Braun; Inés Hernández-Fisac; Rafael Martín-Del-Río; Jorge Tamarit-Rodriguez We have demonstrated recently that branched-chain α-keto acid stimulation of insulin secretion is dependent on islet GABA (γ-aminobutyric acid) metabolism: GABA transamination to succinic...

View article titled, Glucose promotion of GABA metabolism contributes to the stimulation of insulin secretion in β-cells

Includes: Supplementary data

Articles

Zn(II) ions co-secreted with insulin suppress inherent amyloidogenic properties of monomeric insulin

Andra Noormägi, Julia Gavrilova, Julia Smirnova, Vello Tõugu, Peep Palumaa

Biochem J (2010) 430 (3): 511–518.

https://doi.org/10.1042/BJ20100627

Published: 27 August 2010

...Andra Noormägi; Julia Gavrilova; Julia Smirnova; Vello Tõugu; Peep Palumaa Insulin, a 51-residue peptide hormone, is an intrinsically amyloidogenic peptide, forming amyloid fibrils in vitro . In the secretory granules, insulin is densely packed together with Zn(II) into crystals of Zn 2 Insulin 6...

View article titled, Zn(II) ions co-secreted with insulin suppress inherent amyloidogenic properties of monomeric insulin

Articles

Sterol-regulatory-element-binding protein 1c mediates the effect of insulin on the expression of Cidea in mouse hepatocytes

Rui Wang, Xingxing Kong, Anfang Cui, Xiaojun Liu, Ruolan Xiang, Yanli Yang, Youfei Guan, Fude Fang, Yongsheng Chang

Biochem J (2010) 430 (2): 245–254.

https://doi.org/10.1042/BJ20100701

Published: 13 August 2010

... with the induction of Cidea expression by insulin and TO901317 in wild-type mouse hepatocytes, the stimulatory effects were lost in hepatocytes prepared from SREBP-1c -null mice. Adenovirus-mediated overexpression of Cidea in hepatocytes promoted lipid accumulation and triacylglycerol (triglyceride) storage; however...

View article titled, Sterol-regulatory-element-binding protein 1c mediates the effect of insulin on the expression of Cidea in mouse hepatocytes

Articles

Insulin-stimulated phosphorylation of endothelial nitric oxide synthase at serine-615 contributes to nitric oxide synthesis

Stuart A. Ritchie, Christine F. Kohlhaas, Alasdair R. Boyd, Krishna C. Yalla, Kenneth Walsh, John M. C. Connell, Ian P. Salt

Biochem J (2010) 426 (1): 85–90.

https://doi.org/10.1042/BJ20091580

Published: 27 January 2010

...Stuart A. Ritchie; Christine F. Kohlhaas; Alasdair R. Boyd; Krishna C. Yalla; Kenneth Walsh; John M. C. Connell; Ian P. Salt Insulin stimulates endothelial NO (nitric oxide) synthesis via PKB (protein kinase B)/Akt-mediated phosphorylation and activation of eNOS (endothelial NO synthase) at Ser...

View article titled, Insulin-stimulated phosphorylation of endothelial nitric oxide synthase at serine-615 contributes to nitric oxide synthesis

Articles

Transcriptional and post-translational regulation of adiponectin

Meilian Liu, Feng Liu

Biochem J (2010) 425 (1): 41–52.

https://doi.org/10.1042/BJ20091045

Published: 14 December 2009

... distinct roles in the regulation of energy homoeostasis. The serum levels of adiponectin are negatively correlated with obesity and insulin resistance, yet the underlying mechanisms remain elusive. In the present review, we summarize recent progress made on the mechanisms regulating adiponectin gene...

View article titled, Transcriptional and post-translational regulation of adiponectin

Includes: Multimedia, Supplementary data

Articles

Differential regulation of adipocyte PDE3B in distinct membrane compartments by insulin and the β 3 -adrenergic receptor agonist CL316243: effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes

Faiyaz Ahmad, Rebecka Lindh, Yan Tang, Iida Ruishalme, Anita Öst, Bobby Sahachartsiri, Peter Strålfors, Eva Degerman, Vincent C. Manganiello

Biochem J (2009) 424 (3): 399–410.

https://doi.org/10.1042/BJ20090842

Published: 10 December 2009

...Faiyaz Ahmad; Rebecka Lindh; Yan Tang; Iida Ruishalme; Anita Öst; Bobby Sahachartsiri; Peter Strålfors; Eva Degerman; Vincent C. Manganiello In adipocytes, PDE3B (phosphodiesterase 3B) is an important regulatory effector in signalling pathways controlled by insulin and cAMP-increasing hormones...

View article titled, Differential regulation of adipocyte PDE3B in distinct membrane compartments by insulin and the β 3 -adrenergic receptor agonist CL316243: effects of caveolin-1 knockdown on formation/maintenance of macromolecular signalling complexes

Includes: Supplementary data

Articles

Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion

Lynley D. Pound, Suparna A. Sarkar, Richard K. P. Benninger, Yingda Wang, Adisak Suwanichkul, Melanie K. Shadoan, Richard L. Printz, James K. Oeser, Catherine E. Lee, David W. Piston, Owen P. McGuinness, John C. Hutton, David R. Powell, Richard M. O'Brien

Biochem J (2009) 421 (3): 371–376.

https://doi.org/10.1042/BJ20090530

Published: 15 July 2009

... zinc transporter ZnT-8, which provides zinc for insulin-hexamer formation. Polymorphic variants in amino acid residue 325 of human ZnT-8 are associated with altered susceptibility to Type 2 diabetes and ZnT-8 autoantibody epitope specificity changes in Type 1 diabetes. To assess the physiological...

View article titled, Deletion of the mouse Slc30a8 gene encoding zinc transporter-8 results in impaired insulin secretion

Includes: Supplementary data

Articles

Retinoids synergize with insulin to induce hepatic Gck expression

Guoxun Chen, Yan Zhang, Danhong Lu, Nan-qian Li, A. Catharine Ross

Biochem J (2009) 419 (3): 645–653.

https://doi.org/10.1042/BJ20082368

Published: 14 April 2009

... expression. Lipophilic molecules were extracted from rat livers, saponified and re-constituted as an LE (lipophilic extract). LE synergized with insulin to induce primary hepatocyte, but not β-cell, Gck expression in an SREBP-1c (sterol-regulatory-element-binding protein-1c)-independent manner. The dramatic...

View article titled, Retinoids synergize with insulin to induce hepatic Gck expression

Articles

Branched-chain 2-oxoacid transamination increases GABA-shunt metabolism and insulin secretion in isolated islets

Javier Pizarro-Delgado, Inés Hernández-Fisac, Rafael Martín-Del-Río, Jorge Tamarit-Rodriguez

Biochem J (2009) 419 (2): 359–368.

https://doi.org/10.1042/BJ20081731

Published: 27 March 2009

...Javier Pizarro-Delgado; Inés Hernández-Fisac; Rafael Martín-Del-Río; Jorge Tamarit-Rodriguez We have previously shown that oxo-4-methylpentanoate promotes islet GABA (γ-aminobutyric acid) metabolism and stimulates insulin secretion. The main aim of this work was to explore the participation...

View article titled, Branched-chain 2-oxoacid transamination increases GABA-shunt metabolism and insulin secretion in isolated islets

Articles

GAPDH binds GLUT4 reciprocally to hexokinase-II and regulates glucose transport activity

Hilal Zaid, Ilana Talior-Volodarsky, Costin Antonescu, Zhi Liu, Amira Klip

Biochem J (2009) 419 (2): 475–484.

https://doi.org/10.1042/BJ20081319

Published: 27 March 2009

...Hilal Zaid; Ilana Talior-Volodarsky; Costin Antonescu; Zhi Liu; Amira Klip Dietary glucose is taken up by skeletal muscle through GLUT4 (glucose transporter 4). We recently identified by MS proteins displaying insulin-dependent co-precipitation with Myc-tagged GLUT4 from L6 myotubes, including...

View article titled, GAPDH binds GLUT4 reciprocally to hexokinase-II and regulates glucose transport activity

Includes: Supplementary data

Articles

Insulin and epidermal growth factor suppress basal glucose-6-phosphatase catalytic subunit gene transcription through overlapping but distinct mechanisms

Hiroshi Onuma, James K. Oeser, Bryce A. Nelson, Yingda Wang, Brian P. Flemming, Lawrence A. Scheving, William E. Russell, Richard M. O'brien

Biochem J (2009) 417 (2): 611–620.

https://doi.org/10.1042/BJ20080999

Published: 23 December 2008

...-phosphate to glucose. We show here that, in HepG2 hepatoma cells, EGF (epidermal growth factor) inhibits basal mouse G6Pase fusion gene transcription. Several studies have shown that insulin represses basal mouse G6Pase fusion gene transcription through FOXO1 (forkhead box O1), but Stoffel and colleagues...

View article titled, Insulin and epidermal growth factor suppress basal glucose-6-phosphatase catalytic subunit gene transcription through overlapping but distinct mechanisms

Articles

Cytoplasmic polyadenylation-element-binding protein (CPEB)1 and 2 bind to the HIF-1 α mRNA 3′-UTR and modulate HIF-1α protein expression

Sonja Hägele, Uwe Kühn, Melanie Böning, Dörthe M. Katschinski

Biochem J (2009) 417 (1): 235–246.

https://doi.org/10.1042/BJ20081353

Published: 12 December 2008

...) of HIF-1 α mRNA. Overexpression of CPEB1 and CPEB2 affected HIF-1α protein levels mediated by the 3′-UTR of HIF-1 α mRNA. Stimulation of neuroblastoma SK-N-MC cells with insulin and thus activation of endogenous CPEBs increased the expression of a luciferase reporter gene fused to the 3′-UTR of HIF-1 α...

View article titled, Cytoplasmic polyadenylation-element-binding protein (CPEB)1 and 2 bind to the HIF-1 α mRNA 3′-UTR and modulate HIF-1α protein expression

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