... in the mode of oligonucleotide binding by the two Zα domains originating from different proteins. Site-directed mutagenesis was employed together with isothermal titration calorimetry to tease apart finer details of the binding thermodynamics. Our work advances the understanding on binding thermodynamics...

... domain to antagonize host interferon responses. We examined the NS5 PBM motif interactions with all Scribble PDZ domains using isothermal titration calorimetry, which revealed that the proposed internal PBM did not interact with any Scribble PDZ domains. Instead, the C-terminal PBM of NS5 interacted...

... 2021 25 6 2021 25 6 2021 © 2021 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2021 Isothermal titration calorimetry (ITC) assays were used to quantify the binding of MLH1-R472K, MLH1-E475A, MLH1-S467A and MLH1-R470A NLS peptides...

... resonance and isothermal titration calorimetry, and to immobilized heparin and heparan sulfate using surface plasmon resonance. These data, in conjunction with molecular docking, indicate that the binding characteristics such as geometry and stoichiometry of the heterodimer are different between free...

... Society 2020 Biacore epitope glycosylation isothermal titration calorimetry protein–protein interactions surface plasmon resonance The envelope glycoprotein (Env) of HIV-1 is the important for recognition of virus by the receptor of host T-cells (CD4, CCR5 and CXCR4) [ 1 ]. The initial...

... of the C. tropicalis cell wall was damaged. The interaction between CGA-N12 and KRE9 was analyzed by isothermal titration calorimetry (ITC). The results showed that their interaction process was involved an endothermic reaction, and the interaction force was mainly hydrophobic with a few electrostatic...

... , whereas the ‘linchpin’ arginine is found at the ‘+1’ position ( Figure 1B ). © 2019 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2019 E2 enzymes isothermal titration calorimetry oligomerization protein–protein interactions ubiquitin ring E3 ligases...

... License 4.0 (CC BY) . Isothermal titration calorimetries (ITC) were performed on a VP-ITC microcalorimeter (MicroCal, Inc., Northampton, U.S.A.) or AffinityITC LV (TA Instruments, U.S.A.) at 25°C. All protein samples used in ITC experiments were subjected to a final purification step over...

...-Ho Lee ( [email protected] ) 8 7 2016 15 8 2016 22 8 2016 22 8 2016 © 2016 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2016 electron transfer complex enzyme activity ferredoxin isothermal titration calorimetry...

.... Although CUTr1 and the homeodomain, but not CUTr2, are known to contribute to DNA binding, different research groups have not reached a consensus on which DBD is responsible for recognition of the target sequence in MAR, 5′-TAATA-3′. Here, we used isothermal titration calorimetry to demonstrate that CUTr1...

... resin COSMOGEL His-Accept was purchased from Nacalai Tesque. Sephacryl S-100 HR was from GE Healthcare. All other reagents were of analytical grade. CBM32 chitosan crystal structure NMR spectroscopy isothermal titration calorimetry site-directed mutagenesis Interactions between soil...

... hydrogen–deuterium exchange mass spectrometry isothermal titration calorimetry nuclear magnetic resonance polycystin-2 ADPKD (autosomal dominant polycystic kidney disease) is one of the most common genetic kidney disorders, characterized by the progressive development of renal cysts, ultimately...

...-CBM structure, suggesting that it does not form a specific interaction. The orientation of gBCD bound to β1- and β2-CBM is supported by thermodynamic and kinetic data obtained through isothermal titration calorimetry (ITC) and NMR. These results suggest that AMPK containing the muscle-specific β2...

..., G430C and G476R) on the structural and target recognition properties of Keap1 by using nuclear magnetic resonance (NMR) spectroscopy, circular dichroism (CD) and isothermal titration calorimetry (ITC). Depending on their locations in the protein, these mutations are found to exert differential effects...

... of cognitive disorders, e.g. Alzheimer's disease. These modulators bind within the dimer interface of the LBD (ligand-binding domain) and stabilize the agonist-bound conformation slowing receptor desensitization and/or deactivation. In the present study, we employ isothermal titration calorimetry to determine...

... deletion mutants of 4E-BPn ( n =1–3) subtypes were investigated by SPR (surface plasmon resonance) analysis and ITC (isothermal titration calorimetry). Consequently, the conserved PGVTS/T motif within the C-terminal region was shown to act as the second binding region and to play an important role...

...-chloro-1,1,1-trifluoroethane and propofol, to wtHSAd3 and Y411Wd3 was determined using isothermal titration calorimetry, HX and intrinsic tryptophan fluorescence quenching. Although the expressed fragments are less stable than intact wtHSA as indicated by both CD and HX, they retain the secondary...

... ferrochelatase and mesoporphyrin were determined using isothermal titration calorimetry, which revealed a stoichiometry of one molecule of mesoporphyrin bound per protein monomer. The binding is strongly exothermic, with a large intrinsic enthalpy (Δ H =−97.1 kJ · mol −1 ), and is associated with the uptake...

..., determined by isothermal titration calorimetry are similar for zinc binding to E. coli ZiPD ( K d1 =2.2±0.2 μM and K d2 =23.0±0.6 μM) and to the E. coli FlRd metallo-β-lactamase domain ( K d1 =0.7±0.1 μM and K d2 =26.0±0.1 μM). In good correspondence, apo-ZiPD requires incubation with 10 μM zinc for full...

...-1 (human class Alpha GST with two type 1 subunits) and a non-substrate anionic ligand, BSP (bromosulphophthalein), was studied by isothermal titration calorimetry and inhibition kinetics. The binding isotherm is biphasic, best described by a set of two independent sites: a high-affinity site...

... in E. coli GlxI, several approaches were undertaken. Kinetic studies indicate that the catalytic metal ion affects the k cat without significantly affecting the K m for the substrate. Inductively coupled plasma analysis and isothermal titration calorimetry confirmed one metal ion bound to the enzyme...

... GST with two type-1 subunits (hGSTA1-1) and its ΔPhe-222 deletion mutant were studied by isothermal titration calorimetry. The stoichiometry of binding to both proteins is one ANS molecule per GST subunit with a greater affinity for the wild-type ( K d =65 μ M) than for the ΔPhe-222 mutant ( K d =105...

... the non-native polypeptide to Hsp70. 1 To whom correspondence should be addressed (e-mail [email protected] ). 8 8 2001 1 10 2001 25 10 2001 The Biochemical Society, London ©2002 2002 AFM atomic force microscopy isothermal titration calorimetry ITC protein folding...

... The Biochemical Society, London © 1999 1999 heat capacity isothermal titration calorimetry peptide fragments stopped-flow kinetics Biochem. J. (1999) 339, 721 727 (Printed in Great Britain) 721 Thermodynamic and kinetic analysis of the Escherichia coli thioredoxin-C« fragment complementation system...

... suggests that two lactose molecules bind to one molecule of abrin II at pH 7.2. The presence of two binding sites for lactose on the abrin II molecule is also indicated by isothermal titration calorimetry. Plotting Δ H m (the molar transition enthalpy at T m ) against T m yielded values for Δ C p (change...