..., perspectives on how fluorescent biosensors will continue enhancing cell signaling research are discussed. cellular localization fluorescent biosensors intracellular signaling kinases organelles Cell signaling broadly describes how cells process and transmit information originating from...

...Eliza Störmer; Jui-Hung Weng; Jian Wu; Daniela Bertinetti; Pallavi Kaila Sharma; Wen Ma; Friedrich W. Herberg; Susan S. Taylor LRR-kinase 2 (LRRK2) is a multi-domain protein with three catalytically inert N-terminal domains (NtDs) and four C-terminal domains, including a kinase and a GTPase domain...

... pluripotency as observed in the prototypical Protein Kinase A (PKA) as well as in other signaling systems, from typical multidomain signaling proteins to bacterial enzymes. We identify key drivers of pluripotent allostery and illustrate how hypothesizing allosteric pluripotency may solve apparent discrepancies...

... proteolysis and kinase-mediated phosphorylation, enabling integration of signals from multiple pathways into the decision to commit to apoptosis. A new study from Maluch et al. examines how phosphorylation within caspase cleavage sites impacts the efficiency of substrate cleavage. The results demonstrate...

...Ravi Jain; Sakshi Gupta; Manoj Munde; Soumya Pati; Shailja Singh Upon Plasmodium falciparum merozoites exposure to low [K + ] environment in blood plasma, there is escalation of cytosolic [Ca 2+ ] which activates Ca 2+ -Dependent Protein Kinase 1 (CDPK1), a signaling hub of intra-erythrocytic...

...Matthew J. Byrne; Nazia Nasir; Christine Basmadjian; Chitra Bhatia; Rory F. Cunnison; Katherine H. Carr; Corine Mas-Droux; Sharon Yeoh; Céline Cano; Richard Bayliss Nek7 is a serine/threonine-protein kinase required for proper spindle formation and cytokinesis. Elevated Nek7 levels have been...

... variety of disease states. It is regulated through the activity of specific kinases, in particular Unc-51 like autophagy activating kinase 1 (ULK1) and Phosphatidylinositol 3-kinase vacuolar protein sorting 34 (VPS34), which have both been suggested as potential targets for drug development. To identify...

...Alexia F. Kalogeropulou; Jing Zhao; Marc F. Bolliger; Anna Memou; Shreya Narasimha; Tyler P. Molitor; William H. Wilson; Hardy J. Rideout; R. Jeremy Nichols Autosomal-dominant, missense mutations in the leucine-rich repeat protein kinase 2 ( LRRK2 ) gene are the most common genetic predisposition...

...Alexander S. Moffett; Diwakar Shukla Eukaryotic protein kinases (PKs) are a large family of proteins critical for cellular response to external signals, acting as molecular switches. PKs propagate biochemical signals by catalyzing phosphorylation of other proteins, including other PKs, which can...

...Nazma Malik; Thomas Macartney; Annika Hornberger; Karen E. Anderson; Hannah Tovell; Alan R. Prescott; Dario R. Alessi Derailment of the PI3K-AGC protein kinase signalling network contributes to many human diseases including cancer. Recent work has revealed that the poorly studied AGC kinase family...

...-inducing kinase (NIK), IκB kinase (IKK)-1 and IKK-2 in the BJAB B-cell line. The overexpression of TRAF-6 or NIK was sufficient to activate NF-κB and the Cε promoter, whereas their dominant-negative counterparts decreased the ability of CD40 to activate NF-κB and the Cε promoter. The overexpression of wild...

...Hongwei QIAN; Luisa PIPOLO; Walter G. THOMAS Protein kinase C (PKC) phosphorylates the C-terminus of the type 1 angiotensin II receptor (AT 1 ), although the exact site(s) of phosphorylation are unidentified. In the present study, we examined the phosphorylation of epitope-tagged wild-type AT 1A...

...Yang FENG; David R. HODGE; Giuseppe PALMIERI; Dan L. CHASE; Dan L. LONGO; Douglas K. FERRIS The polo-like kinase (Plk) family has been shown to have an important role in the regulation of the cell-division cycle, especially in organization of the spindle structure, in species from fungi to humans...