1-50 of 66
Keywords: kinetics
Close
Follow your search
Access your saved searches in your account

Would you like to receive an alert when new items match your search?
Close Modal
Sort by
Articles
Biochem J (2020) 477 (8): 1443–1457.
Published: 27 April 2020
... binding could be inhibited competitively using previously published ASCT2 inhibitors [ 38 ]. We propose a mechanism for interaction of ASCT2 with various protonation states of L-DAP ( Figure 1D–G ), based on kinetic modeling as well as molecular docking. Overall, these findings extend the substrate...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (19): 2677–2703.
Published: 11 October 2019
... kinetics and mechanisms. This work attempts to provide the most comprehensive summary, to date, of the available experimental data on amyloid fibril elongation rate constants and the temperature and concentration dependence of amyloid fibril elongation rates. These data are compared with those from other...
Articles
Articles
Articles
Biochem J (2016) 473 (4): 383–396.
Published: 09 February 2016
... at 100K × g for 30 min. Supernatant and pellet fractions were separated by SDS/PAGE and quantified as stated for the binding assay. actin cross-linking kinetics nucleation polymerization Palladin is a F-actin [filamentous actin] binding protein that is involved in both normal...
Articles
Biochem J (2015) 472 (3): 329–338.
Published: 27 November 2015
... an oligomerization mechanism to recognize its physiological protein substrates. docking kinase kinetics oligomerization phosphorylation splicing Protein phosphorylation regulates countless cellular processes including the fundamental conversion of genetic information into functioning proteins...
Articles
Articles
Biochem J (2015) 466 (2): 243–251.
Published: 20 February 2015
.... According to the kinetic data, the catalytic efficiency ( k cat /K M ) of mBChE against (−)-cocaine is comparable with that of hBChE, but the catalytic efficiency of mCocH against (−)-cocaine is remarkably lower than that of hCocH by ~250-fold. The remarkable difference in the catalytic activity between...
Articles
Biochem J (2014) 463 (1): 135–144.
Published: 08 September 2014
... of the escape. Perturbation of core promoter–polymerase contacts had opposing effects on the rates of melting and escape, demonstrating a direct role of core promoter elements sequence in setting not only the kinetics of promoter melting, but also the kinetics of promoter escape. The start of RNA synthesis...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (1): 143–152.
Published: 24 July 2014
... kinase 1 (CLK1) docking kinase kinetics phosphorylation splicing As essential regulators of cell function, protein kinases phosphorylate select substrate targets on serine, threonine or tyrosine. This post-translational modification may alter the conformation and/or the biological activity...
Includes: Supplementary data
Articles
Biochem J (2014) 460 (3): 447–457.
Published: 29 May 2014
..., for the first time, in comparison with those for cocaine. On the basis of the obtained kinetic data, wild-type human BChE has a lower catalytic activity for cocaethylene ( k cat =3.3 min −1 , K M =7.5 μM and k cat / K M =4.40×10 5 M −1 ·min −1 ) compared with its catalytic activity for (−)-cocaine. E14-3...
Articles
Biochem J (2014) 457 (1): 197–206.
Published: 10 December 2013
.... On the basis of the obtained kinetic data, wild-type human BChE has a significantly lower catalytic activity for norcocaine ( k cat =2.8 min −1 , K m =15 μM and k cat / K m =1.87×10 5 M −1 ·min −1 ) compared with its catalytic activity for (−)-cocaine. The BChE mutants examined in the present study have...
Articles
Biochem J (2014) 457 (1): 137–149.
Published: 10 December 2013
... CUB domains inhibit angiogenesis in vitro . affinity extracellular matrix kinetics network protein–protein interaction surface plasmon resonance PCPE-1 (procollagen C-proteinase enhancer-1) and PCPE-2 are two closely related ECM (extracellular matrix) glycoproteins that, although...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 337–344.
Published: 12 July 2013
... address: Biological and Biomedical Sciences Graduate Program, Yale University, New Haven, CT 06520, U.S.A. activation aggregation dimer epidermal growth factor receptor (EGFR) fibril kinetics The kinase domain of the EGFR (epidermal growth factor receptor; also known as ErbB1...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 427–434.
Published: 12 July 2013
...Heidi L. H. Malaby; William R. Kobertz Type I transmembrane peptides acquire N-linked glycans during and after protein synthesis to facilitate anterograde trafficking through the secretory pathway. Mutations in N-glycosylation consensus sites (NXT and NXS, where X≠P) that alter the kinetics...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 491–496.
Published: 14 December 2012
... of the large Fe(II)/2-oxoglutarate- dependent family of human histone demethylases. In the present study we report kinetic studies on the reaction of KDM4E with O 2 . Steady-state assays showed that KDM4E has a graded response to O 2 over a physiologically relevant range of O 2 concentrations. Pre-steady state...
Includes: Supplementary data
Articles
Biochem J (2012) 446 (3): 499–508.
Published: 28 August 2012
.... In the present study we tested the hypothesis that storage of leucodepleted RBCs results in cells that inhibit NO (nitric oxide) signalling more so than younger cells. Using competition kinetic analyses and protocols that minimized contributions from haemolysis or microparticles, our data indicate...
Includes: Supplementary data
Articles
Articles
Biochem J (2012) 443 (2): 505–514.
Published: 27 March 2012
... in the PBD under code 3FC5. 1 To whom correspondence should be addressed (email [email protected] ). 12 9 2011 17 1 2012 3 2 2012 3 2 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 electron transfer kinetics nitric oxide synthase (NOS...
Includes: Supplementary data
Articles
Articles
Biochem J (2011) 440 (2): 263–271.
Published: 14 November 2011
... investigated the kinetics of interaction between native Rh and G t in different conditions by surface plasmon resonance and analysed the results in the general physiological context by employing a holistic systems modelling approach. The results from the present study point to a mechanism that is intermediate...
Articles
Biochem J (2011) 439 (3): 423–434.
Published: 13 October 2011
...-determined the kinetic constants of the reactions of tempol with MPO-I ( k =3.5×10 5 M −1 ·s −1 ) and MPO-II, the kinetics of which indicated a binding interaction ( K =2.0×10 −5 M; k =3.6×10 −2 s −1 ). Also, we showed that tempol reacts extremely slowly with hypochlorous acid ( k =0.29 and 0.054 M −1 ·s −1...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library © The Authors Journal compilation © 2011 Biochemical Society 2011 Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small antigenic peptides bound...
Includes: Supplementary data
Articles
Biochem J (2010) 432 (3): 485–494.
Published: 25 November 2010
... 3A4 (CYP3A4) electron transfer kinetics lateral diffusion reductase The cytochrome P450 mono-oxygenase system in the endoplasmic reticulum, particularly in the liver, lung and small intestine, plays a central role in the metabolism of drugs and other xenobiotics. The members...
Includes: Supplementary data
Articles
Articles
Biochem J (2010) 425 (2): 353–360.
Published: 23 December 2009
... 10 2009 22 10 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 adaptation enzyme Equilibrium Model evolution kinetics temperature The way enzymes respond to temperature is fundamental to many areas of biology. Until recently, the effect...
Articles
Biochem J (2009) 422 (1): 111–117.
Published: 29 July 2009
.... Although it is established that HOSCN selectively targets thiols, absolute kinetic data for the reactions of thiols with HOSCN are absent from the literature. This study shows for the first time that the reactions of HOSCN with low-molecular-mass thiol residues occur with rate constants in the range from...
Articles
Articles
Articles
Articles
Biochem J (2009) 417 (1): 355–360.
Published: 12 December 2008
... of INCENP on the activation kinetics of AurB and also elucidated the kinetic mechanism of AurB-catalysed substrate phosphorylation. We have concluded that: (i) substoichoimetric concentrations of INCENP are sufficient for AurB autophosphorylation at the activation loop residue Thr 232 , and hence INCENP...
Articles
Articles
Biochem J (2008) 413 (3): 369–387.
Published: 15 July 2008
... of lactating cows stimulates expression of hepatic PC, indicating an adaptive response to the demand of hepatic gluconeogenesis [ 20 ]. 7 4 2008 7 5 2008 7 5 2008 © The Authors Journal compilation © 2008 Biochemical Society 2008 acetyl-CoA biotin gene expression kinetics...
Articles
Biochem J (2008) 409 (3): 731–740.
Published: 15 January 2008
... by promoting uptake at the cell surface. Kinetic analyses showed that hCtr2–GFP stimulated saturable copper uptake with a K m of 11.0±2.5 μM and a K 0.5 of 6.9±0.7 μM when data were fitted to a rectangular hyperbola or Hill equation respectively. Competition experiments revealed that silver completely...
Articles
Biochem J (2007) 402 (3): 439–447.
Published: 26 February 2007
... to function as Arf effectors and oncogenes. We have set out to characterize the kinetics of the GAP-induced GTP hydrolysis using a truncated form of ASAP1 [Arf GAP with SH3 (Src homology 3) domain, ankyrin repeats and PH (pleckstrin homology) domains 1] as a model. We found that ASAP1 used Arf1-GTP...
Includes: Supplementary data
Articles
Biochem J (2007) 402 (3): 503–513.
Published: 26 February 2007
... fragmentation kinetics, activated whole MMP-2 or plasmin were added to fibrinogen solutions at a final concentration of 10 pM, whereas the catalytic domain of MMP-2 was added to fibrinogen solutions at a final concentration of 15–20 pM. The kinetics of MMP-2 were performed in 50 mM Tris/HCl, 0.1 M NaCl and 10...
Articles
Biochem J (2007) 402 (2): 331–337.
Published: 12 February 2007
... value in the pure and applied study of enzymes. enzyme activity Equilibrium Model kinetics stability thermal-dependence The effect of temperature on enzyme activity has been described by two well-established thermal parameters: the Arrhenius activation energy, which describes...
Articles
Biochem J (2007) 401 (2): 485–495.
Published: 21 December 2006
... illustrate a simple kinetic model of G-protein signalling. This model shows that stable production of significant amounts of free Gα GTP (GTP-bound Gα subunit) and βγ is only one of multiple modes of behaviour of the G-protein system upon activation. Other modes, previously uncharacterized, are sustained...
Includes: Supplementary data
Articles
Biochem J (2006) 400 (1): 13–22.
Published: 27 October 2006
...) Journal of Biological Chemistry 276 , 18024–18030]. A detailed kinetic analysis of the H121A mutant enzyme shows that the decrease in turnover number is largely due to a corresponding decrease in the rate constant of flavin reduction, whilst the re-oxidation reaction is only marginally altered...
Articles
Articles
Articles
Biochem J (2006) 394 (1): 259–265.
Published: 27 January 2006
... through the measurement of the temperature dependence of the reaction rates and the kinetic isotope effects. Single turnover experiments at pH 7.0 revealed a strong dependence of the reaction rates on temperature. The observed relatively large difference in the activation energies for hydrogen...
Includes: Supplementary data
Articles
Articles
Articles
Articles
Articles
Biochem J (2003) 376 (3): 733–740.
Published: 15 December 2003
...Suzanne L. JACQUES; Athan KULIOPULOS Thrombin activation of human platelets is mediated by the high-affinity PAR1 (protease-activated receptor-1) and the low-affinity PAR4 receptor. PAR1 and PAR4 exhibit markedly disparate kinetics of activation that likely reflect differences in the macromolecular...
Articles
Biochem J (2003) 374 (2): 529–535.
Published: 01 September 2003
... temperature of DHFR from E. coli . The turnover and the hydride-transfer rates in the kinetic scheme of TmDHFR were derived from measurements of the steady-state and pre-steady-state kinetics using absorbance and stopped-flow fluorescence spectroscopy. The rate constant for hydride transfer was found...
Articles
Biochem J (2003) 370 (1): 245–253.
Published: 15 February 2003
... hydrogen bond, leading to a higher oxygen-dissociation rate. However, Yoldia haemoglobin has the usual distal and proximal histidines, so the primary structure alone does not provide an obvious explanation for the low affinity. The CO-binding kinetics are biphasic, with the fraction of slow phase...
Articles
Biochem J (2002) 367 (1): 157–162.
Published: 01 October 2002
... 28 6 2002 10 7 2002 10 7 2002 The Biochemical Society, London ©2002 2002 kinetics phospholipid biosynthesis regulation substrate specificity Abbreviations used: C 16 , palmitic acid; C 19 , tuberculostearic acid; CDP-DAG, cytidine diphosphate-diacylglycerol; CDS...