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Keywords: kinetics
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Articles
Biochem J (2020) 477 (8): 1443–1457.
Published: 27 April 2020
... 2019 16 3 2020 3 4 2020 3 4 2020 © 2020 The Author(s). Published by Portland Press Limited on behalf of the Biochemical Society 2020 ASCT2 docking electrophysiology kinetics neutral amino acid transporter substrate selectivity The alanine serine cysteine...
Includes: Supplementary data
Articles
Biochem J (2019) 476 (19): 2677–2703.
Published: 11 October 2019
... kinetics and mechanisms. This work attempts to provide the most comprehensive summary, to date, of the available experimental data on amyloid fibril elongation rate constants and the temperature and concentration dependence of amyloid fibril elongation rates. These data are compared with those from other...
Articles
Biochem J (2017) 474 (10): 1689–1703.
Published: 04 May 2017
... electron microscopy kinetics Nicotiana tabacum quaternary structure suspension cells Saccharomyces cerevisiae has been used as a fungal model to study PDR transporters, which are all localized to the plasma membrane. Some of them confer resistance to a large number of drugs and xenobiotics...
Includes: Supplementary data
Articles
Biochem J (2016) 473 (4): 411–421.
Published: 09 February 2016
... Prx3 due to slower dimerization of the sulfenic acid (S p OH) intermediate. Four variant amino acids near the C-terminus have been shown to contribute to this difference. We have performed kinetic analysis of the relationship between hyperoxidation and disulfide formation, using whole-protein MS...
Articles
Biochem J (2016) 473 (4): 383–396.
Published: 09 February 2016
... 23 11 2015 25 11 2015 25 11 2015 actin cross-linking kinetics nucleation polymerization © 2016 Authors; published by Portland Press Limited 2016 Our results indicate palladin stimulates actin polymerization by accelerating the nucleation step, while also enhancing...
Articles
Biochem J (2015) 472 (3): 329–338.
Published: 27 November 2015
... 2015 CLK1 (Cdc (cell division cycle)2-like kinase 1) uses an oligomerization mechanism to recognize its physiological protein substrates. docking kinase kinetics oligomerization phosphorylation splicing Protein phosphorylation regulates countless cellular processes including...
Articles
Biochem J (2015) 468 (2): 271–282.
Published: 22 May 2015
... CXXC active site motif involved in disulfide exchange reaction. In the human pathogenic Neisseria meningitidis species, no TlpA has been characterized so far. In the present study, using an in silico analysis, we identified a putative periplasmic TlpA, called TlpA2. Biochemical and kinetic...
Includes: Supplementary data
Articles
Biochem J (2015) 466 (2): 243–251.
Published: 20 February 2015
.... According to the kinetic data, the catalytic efficiency ( k cat /K M ) of mBChE against (−)-cocaine is comparable with that of hBChE, but the catalytic efficiency of mCocH against (−)-cocaine is remarkably lower than that of hCocH by ~250-fold. The remarkable difference in the catalytic activity between...
Articles
Biochem J (2014) 463 (1): 135–144.
Published: 08 September 2014
... of the escape. Perturbation of core promoter–polymerase contacts had opposing effects on the rates of melting and escape, demonstrating a direct role of core promoter elements sequence in setting not only the kinetics of promoter melting, but also the kinetics of promoter escape. The start of RNA synthesis...
Includes: Supplementary data
Articles
Biochem J (2014) 462 (1): 143–152.
Published: 24 July 2014
... hyperphosphorylation of SR proteins. Cdc2-like kinase 1 (CLK1) docking kinase kinetics phosphorylation splicing As essential regulators of cell function, protein kinases phosphorylate select substrate targets on serine, threonine or tyrosine. This post-translational modification may alter...
Includes: Supplementary data
Articles
Biochem J (2014) 460 (3): 447–457.
Published: 29 May 2014
..., for the first time, in comparison with those for cocaine. On the basis of the obtained kinetic data, wild-type human BChE has a lower catalytic activity for cocaethylene ( k cat =3.3 min −1 , K M =7.5 μM and k cat / K M =4.40×10 5 M −1 ·min −1 ) compared with its catalytic activity for (−)-cocaine. E14-3...
Articles
Biochem J (2014) 457 (1): 137–149.
Published: 10 December 2013
... CUB domains inhibit angiogenesis in vitro . affinity extracellular matrix kinetics network protein–protein interaction surface plasmon resonance PCPE-1 (procollagen C-proteinase enhancer-1) and PCPE-2 are two closely related ECM (extracellular matrix) glycoproteins that, although...
Includes: Supplementary data
Articles
Biochem J (2014) 457 (1): 197–206.
Published: 10 December 2013
.... On the basis of the obtained kinetic data, wild-type human BChE has a significantly lower catalytic activity for norcocaine ( k cat =2.8 min −1 , K m =15 μM and k cat / K m =1.87×10 5 M −1 ·min −1 ) compared with its catalytic activity for (−)-cocaine. The BChE mutants examined in the present study have...
Articles
Biochem J (2013) 453 (3): 337–344.
Published: 12 July 2013
... Biochemical Society 2013 activation aggregation dimer epidermal growth factor receptor (EGFR) fibril kinetics The kinase domain of the EGFR (epidermal growth factor receptor; also known as ErbB1) is activated by an allosteric mechanism upon receptor dimerization. The C-lobe (C-terminal lobe...
Includes: Supplementary data
Articles
Biochem J (2013) 453 (3): 427–434.
Published: 12 July 2013
...Heidi L. H. Malaby; William R. Kobertz Type I transmembrane peptides acquire N-linked glycans during and after protein synthesis to facilitate anterograde trafficking through the secretory pathway. Mutations in N-glycosylation consensus sites (NXT and NXS, where X≠P) that alter the kinetics...
Includes: Supplementary data
Articles
Biochem J (2013) 449 (2): 491–496.
Published: 14 December 2012
... of the large Fe(II)/2-oxoglutarate- dependent family of human histone demethylases. In the present study we report kinetic studies on the reaction of KDM4E with O 2 . Steady-state assays showed that KDM4E has a graded response to O 2 over a physiologically relevant range of O 2 concentrations. Pre-steady state...
Includes: Supplementary data
Articles
Biochem J (2012) 446 (3): 499–508.
Published: 28 August 2012
.... In the present study we tested the hypothesis that storage of leucodepleted RBCs results in cells that inhibit NO (nitric oxide) signalling more so than younger cells. Using competition kinetic analyses and protocols that minimized contributions from haemolysis or microparticles, our data indicate...
Includes: Supplementary data
Articles
Biochem J (2012) 445 (2): 219–228.
Published: 27 June 2012
... -acetyltransferase detoxification enzyme variant kinetics Legionella pneumophila is an intracellular bacterium that is ubiquitous in aquatic environments, where it is part of multi-organismal biofilms. The environmental life cycle of L. pneumophila includes growth, survival and spreading within...
Includes: Supplementary data
Articles
Biochem J (2012) 443 (2): 505–514.
Published: 27 March 2012
... in the PBD under code 3FC5. 1 To whom correspondence should be addressed (email Simon.Daff@ed.ac.uk ). 12 9 2011 17 1 2012 3 2 2012 3 2 2012 © The Authors Journal compilation © 2012 Biochemical Society 2012 electron transfer kinetics nitric oxide synthase (NOS...
Includes: Supplementary data
Articles
Biochem J (2012) 441 (1): 95–104.
Published: 14 December 2011
... 2012 accessory domain cellulase 5A carbohydrate-binding module kinetics structure thermal stability The production of ethanol from lignocellulosic biomass is well-placed among other possibilities to produce energy, owing to its potential sustainability and agro-economic benefits [ 1...
Includes: Supplementary data
Articles
Biochem J (2011) 440 (2): 263–271.
Published: 14 November 2011
... investigated the kinetics of interaction between native Rh and G t in different conditions by surface plasmon resonance and analysed the results in the general physiological context by employing a holistic systems modelling approach. The results from the present study point to a mechanism that is intermediate...
Articles
Biochem J (2011) 439 (3): 423–434.
Published: 13 October 2011
...-determined the kinetic constants of the reactions of tempol with MPO-I ( k =3.5×10 5 M −1 ·s −1 ) and MPO-II, the kinetics of which indicated a binding interaction ( K =2.0×10 −5 M; k =3.6×10 −2 s −1 ). Also, we showed that tempol reacts extremely slowly with hypochlorous acid ( k =0.29 and 0.054 M −1 ·s −1...
Includes: Supplementary data
Articles
Biochem J (2011) 435 (2): 411–420.
Published: 29 March 2011
... 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 aminopeptidase antigen kinetics peptide specificity site-directed mutagenesis substrate library Cytotoxic T-lymphocytes identify infected or transformed cells by recognizing small antigenic peptides bound...
Includes: Supplementary data
Articles
Biochem J (2010) 432 (3): 485–494.
Published: 25 November 2010
... 29 9 2010 29 9 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 artificial liposome cytochrome P450 cytochrome P450 3A4 (CYP3A4) electron transfer kinetics lateral diffusion reductase The cytochrome P450 mono-oxygenase system in the endoplasmic...
Includes: Supplementary data
Articles
Biochem J (2010) 429 (3): 527–532.
Published: 14 July 2010
... correspondence should be addressed (email betorbet@scripps.edu ). 21 10 2009 8 5 2010 27 5 2010 27 5 2010 © The Authors Journal compilation © 2010 Biochemical Society 2010 anti-retroviral therapy high-diversity chemical library high-throughput screening HIV-1 kinetics non...
Articles
Biochem J (2010) 425 (2): 353–360.
Published: 23 December 2009
... 10 2009 22 10 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 adaptation enzyme Equilibrium Model evolution kinetics temperature The way enzymes respond to temperature is fundamental to many areas of biology. Until recently, the effect...
Articles
Biochem J (2009) 422 (1): 111–117.
Published: 29 July 2009
.... Although it is established that HOSCN selectively targets thiols, absolute kinetic data for the reactions of thiols with HOSCN are absent from the literature. This study shows for the first time that the reactions of HOSCN with low-molecular-mass thiol residues occur with rate constants in the range from...
Articles
Biochem J (2009) 421 (1): 97–106.
Published: 12 June 2009
... displays a peculiar resistance to oxime-mediated reactivation. We investigated the basis of oxime resistance of phosphoramidyl–BChE conjugates by determining the kinetics of inhibition, reactivation (obidoxime {1,1′-(oxybis-methylene) bis[4-(hydroxyimino) methyl] pyridinium dichloride}, TMB-4 [1,3...
Articles
Biochem J (2009) 419 (1): 221–230.
Published: 13 March 2009
... quantitative DNA-binding study of this plant-specific family of proteins. Interactions of full-length proteins AtTRB1 and AtTRB3 with telomeric DNA were analysed by electrophoretic mobility-shift assay, fluorescence anisotropy and surface plasmon resonance to reveal their binding stoichiometry and kinetics...
Includes: Supplementary data
Articles
Biochem J (2009) 418 (3): 605–614.
Published: 25 February 2009
...) kinetics substrate recognition substrate specificity PGs (proteoglycans) represent one of the largest and most complex molecular structures in animals, plants and other kingdoms. They are composed of polysaccharide GAG (glycosaminoglycan) chains covalently bound to a protein core. The different...
Includes: Supplementary data
Articles
Biochem J (2009) 417 (1): 355–360.
Published: 12 December 2008
... of INCENP on the activation kinetics of AurB and also elucidated the kinetic mechanism of AurB-catalysed substrate phosphorylation. We have concluded that: (i) substoichoimetric concentrations of INCENP are sufficient for AurB autophosphorylation at the activation loop residue Thr 232 , and hence INCENP...
Articles
Biochem J (2008) 415 (2): 217–223.
Published: 25 September 2008
... structures of both human and A. fumigatus GNA1, as well as their kinetic characterization. The structures show significant differences in the sugar-binding site with, in particular, several non-conservative substitutions near the phosphate-binding pocket. Mutagenesis targeting these differences revealed...
Articles
Biochem J (2008) 413 (3): 369–387.
Published: 15 July 2008
... kinetics metabolism pyruvate carboxylase structure PC (pyruvate carboxylase; EC 6.4.1.1) was discovered by Utter and Keech [ 1 ] in the course of studies on the intracellular distribution of enzymes involved in the dicarboxylic acid shuttle and its relationship to gluconeogenesis in chicken...
Articles
Biochem J (2008) 409 (3): 731–740.
Published: 15 January 2008
... by promoting uptake at the cell surface. Kinetic analyses showed that hCtr2–GFP stimulated saturable copper uptake with a K m of 11.0±2.5 μM and a K 0.5 of 6.9±0.7 μM when data were fitted to a rectangular hyperbola or Hill equation respectively. Competition experiments revealed that silver completely...
Articles
Biochem J (2007) 402 (3): 439–447.
Published: 26 February 2007
... to function as Arf effectors and oncogenes. We have set out to characterize the kinetics of the GAP-induced GTP hydrolysis using a truncated form of ASAP1 [Arf GAP with SH3 (Src homology 3) domain, ankyrin repeats and PH (pleckstrin homology) domains 1] as a model. We found that ASAP1 used Arf1-GTP...
Includes: Supplementary data
Articles
Biochem J (2007) 402 (3): 503–513.
Published: 26 February 2007
... parameters without altering the main enzymatic mechanism. 1 To whom correspondence should be addressed (email coletta@seneca.uniroma2.it ). 13 7 2006 17 10 2006 3 11 2006 6 11 2006 The Biochemical Society, London 2007 fibrinogen fragmentation gelatinase A kinetics...
Articles
Biochem J (2007) 402 (2): 331–337.
Published: 12 February 2007
... for the application of the Equilibrium Model to non-ideal enzyme reactions. 1 To whom correspondence should be addressed (email r.daniel@waikato.ac.nz ). 27 7 2006 30 10 2006 3 11 2006 3 11 2006 The Biochemical Society, London 2007 enzyme activity Equilibrium Model kinetics...
Articles
Biochem J (2007) 401 (2): 485–495.
Published: 21 December 2006
... illustrate a simple kinetic model of G-protein signalling. This model shows that stable production of significant amounts of free Gα GTP (GTP-bound Gα subunit) and βγ is only one of multiple modes of behaviour of the G-protein system upon activation. Other modes, previously uncharacterized, are sustained...
Includes: Supplementary data
Articles
Biochem J (2006) 400 (1): 13–22.
Published: 27 October 2006
...) Journal of Biological Chemistry 276 , 18024–18030]. A detailed kinetic analysis of the H121A mutant enzyme shows that the decrease in turnover number is largely due to a corresponding decrease in the rate constant of flavin reduction, whilst the re-oxidation reaction is only marginally altered...
Articles
Biochem J (2006) 399 (3): 463–471.
Published: 13 October 2006
... . 1 To whom correspondence should be addressed (email kvandegriff@sangart.com ). 31 5 2006 29 6 2006 3 7 2006 3 7 2006 The Biochemical Society, London 2006 autoxidation haem dissociation kinetics poly-(ethylene glycol) (PEG) PEGylated haemoglobin...
Articles
Biochem J (2006) 396 (1): 117–126.
Published: 26 April 2006
... vector which had been digested with the same enzymes. Clones were isolated and positive clones were sequenced using BS47 as the primer. phosphorylation protein kinase A (PKA) pyruvate kinase 1 (Pyk1) substrate specificity yeast kinetics The intracellular transduction of signals from...
Articles
Biochem J (2006) 394 (1): 259–265.
Published: 27 January 2006
... through the measurement of the temperature dependence of the reaction rates and the kinetic isotope effects. Single turnover experiments at pH 7.0 revealed a strong dependence of the reaction rates on temperature. The observed relatively large difference in the activation energies for hydrogen...
Includes: Supplementary data
Articles
Biochem J (2005) 390 (3): 665–673.
Published: 05 September 2005
... 20 5 2005 27 5 2005 27 5 2005 The Biochemical Society, London 2005 CPD-N (isoform of carboxypeptidase D) cytokine-inducible haematopoietic tumour cell kinetics nuclear enzyme prolactin Rat metallocarboxypeptidase D [CPD (carboxypeptidase D)] is the only known CP...
Articles
Biochem J (2005) 386 (2): 315–324.
Published: 22 February 2005
... molecular and catalytic characterization, including substrate selectivity, kinetics and pH-dependence, has not been conducted. In the present study, DPPII was purified from human seminal plasma to apparent homogeneity with a high yield (40%) purification scheme, including an inhibitor-based affinity...
Articles
Biochem J (2004) 378 (2): 391–397.
Published: 01 March 2004
.... Key words: Ca2+/calmodulin-dependent protein kinase II (CaMKII), kinetics, N-methyl-D-aspartate receptor subunit 2B (NR2B), molecular modelling, phosphorylation, synapse. INTRODUCTION CaMKII (Ca2+/calmodulin-dependent protein kinase II) is a serine/threonine protein kinase involved in long-term...
Articles
Biochem J (2004) 377 (2): 309–316.
Published: 15 January 2004
... in E. coli GlxI, several approaches were undertaken. Kinetic studies indicate that the catalytic metal ion affects the k cat without significantly affecting the K m for the substrate. Inductively coupled plasma analysis and isothermal titration calorimetry confirmed one metal ion bound to the enzyme...
Articles
Biochem J (2003) 376 (3): 733–740.
Published: 15 December 2003
...Suzanne L. JACQUES; Athan KULIOPULOS Thrombin activation of human platelets is mediated by the high-affinity PAR1 (protease-activated receptor-1) and the low-affinity PAR4 receptor. PAR1 and PAR4 exhibit markedly disparate kinetics of activation that likely reflect differences in the macromolecular...
Articles
Biochem J (2003) 374 (2): 529–535.
Published: 01 September 2003
... temperature of DHFR from E. coli . The turnover and the hydride-transfer rates in the kinetic scheme of TmDHFR were derived from measurements of the steady-state and pre-steady-state kinetics using absorbance and stopped-flow fluorescence spectroscopy. The rate constant for hydride transfer was found...
Articles
Biochem J (2003) 370 (1): 245–253.
Published: 15 February 2003
... hydrogen bond, leading to a higher oxygen-dissociation rate. However, Yoldia haemoglobin has the usual distal and proximal histidines, so the primary structure alone does not provide an obvious explanation for the low affinity. The CO-binding kinetics are biphasic, with the fraction of slow phase...
Articles
Biochem J (2002) 367 (1): 157–162.
Published: 01 October 2002
... (IC &! fl 0.3 mM) and the nucleotides ATP, UTP and GTP. This study contributes to the delineation of PL biosynthesis in mycobacteria. Key words: kinetics, phospholipid biosynthesis, regulation, sub- strate specificity. teria, biosynthesis of PA has been studied in M. butyricum (now termed M. smegmatis...