...Christine Y. Chuang; Georg Degendorfer; Astrid Hammer; John M. Whitelock; Ernst Malle; Michael J. Davies ECM (extracellular matrix) materials, such as laminin, perlecan, type IV collagen and fibronectin, play a key role in determining the structure of the arterial wall and the properties of cells...

...Emmanuel Collec; Marie-Christine Lecomte; Wassim El Nemer; Yves Colin; Caroline Le Van Kim Lu/BCAM (Lutheran/basal cell-adhesion molecule) is a laminin 511/521 receptor expressed in erythroid and endothelial cells, and in epithelial tissues. The RK573–574 (Arg 573 -Lys 574 ) motif of the Lu/BCAM...

...-integrin α3 mAb (3G8), anti-integrin α5 mAb (8F1) and normal mouse IgG. Immunoprecipitates were blotted with peroxidase-conjugated streptavidin (Zymed). basement membrane CD81 CD9 cell adhesion integrin α3β1 laminin The tetraspanin superfamily consists of at least 32 members in mammals...

.... We have observed previously that AChE is able to bind to mouse laminin-111 in vitro by an electrostatic mechanism. We have also observed that certain mAbs (monoclonal antibodies) recognizing AChE's PAS (peripheral anionic site) inhibit both laminin binding and cell adhesion in neuroblastoma cells...

... binding to laminin-1 and carbohydrate-dependent mAbs (monoclonal antibodies) IIH6 and VIA4 1 . We digested highly purified skeletal muscle α-dystroglycan with an array of linkage-specific endo- and exoglycosidases, which were verified for action on α-dystroglycan by loss/gain of reactivity for lectins...

... cell down-regulation EphB2 laminin Rap1 EphrinB1, laminin and the EphB2 specific antibodies were obtained from R & D Systems (Wiesbaden, Germany). The ROCK-inhibitor Y-27632 and cytochalasin D were obtained from Calbiochem (Bad Soden, Germany). Alexa-568-conjugated phalloidin...

... of cells on laminin. These findings suggest that Spry4 suppresses cell spreading by inhibiting the kinase activity of TESK1. Although tyrosine phosphorylation is required for the inhibitory activity of Spry4 on a Ras/MAP kinase pathway, mutation of the corresponding tyrosine residue (Tyr-75 in human Spry4...

... of integrin α7β1 has not been explored. In the present study, we show that ADP-ribosylation of integrin α7 takes place exclusively in differentiated myotubes and that this post-translational modification modulates the affinity of α7β1 dimer for its ligand, laminin. ADP-ribosylation in the 37-kDa ‘stalk...

...Hironobu YAMASHITA; Akira GOTO; Tatsuhiko KADOWAKI; Yasuo KITAGAWA We have previously shown that the LG4 (laminin G-like) domain of the laminin α4 chain is responsible for the significantly higher affinity of the α4 chain to heparin than found for other α chains [Yamaguchi, Yamashita, Mori, Okazaki...

... and collagen I was studied in greater detail and could be shown to occur at distinct sites on the collagen I molecule and to have a K D of about 3×10 -8 M. Interactions with some non-collagenous protein ligands were even stronger, with matrilin-2 binding to fibrillin-2, fibronectin and laminin-1—nidogen-1...

... proteins. Key words: cytoskeleton, a-dystroglycan, extracellular matrix, laminin, O-mannosylated protein. ed, e.g. UDP-Gal :GlcNAc-R b1,4-galactosyltransferases [11], UDP-Gal :GlcNAc-R b1,3-galactosyltransferases [11] and GDP- Fuc:Galb1-4GlcNAc-R (Fuc to GlcNAc) a1,3-fucosyltrans- ferases [12] (where R can...