... and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . Open access for this article was enabled by the participation of University of Minnesota in an all-inclusive Read & Publish pilot with Portland Press and the Biochemical Society. apoptosis lectin molecular dynamics...

... have emerged to account for their specific biological meaning. Acting as ‘readers’ of glycan-encoded information, carbohydrate-specific receptors (lectins) are a means to turn the glycans’ potential to serve as signals into a multitude of (patho)physiologically relevant responses. Once the far-reaching...

...Antonio Eufrásio Vieira Neto; Felipe Domingos de Sousa; Humberto D'Muniz Pereira; Frederico Bruno Mendes Batista Moreno; Marcos Roberto Lourenzoni; Thalles Barbosa Grangeiro; Ana Cristina de Oliveira Monteiro Moreira; Renato de Azevedo Moreira Frutalin (FTL) is a multiple-binding lectin belonging...

... structure lectin X-ray crystallography The human genome possesses two genes coding for active chitinases, hCHT (chitotriosidase) and the hAMCase (acidic mammalian chitinase), both from the CAZy GH18 (glycoside hydrolase 18) family [ 1 ]. hCHT is a phagocyte-specific chitinase that exists in two...

... compilation © 2009 Biochemical Society 2009 carbohydrate hydration lectin molecular recognition structural energetics surface area model Heat capacity effects have long been recognized as key to understanding the molecular principles that underpin non-covalent driven processes [ 1 – 4...

...Tesfaldet Tecle; Mitchell R. White; Grith Sorensen; Donald Gantz; Nilgun Kacak; Uffe Holmskov; Kelly Smith; Erika C. Crouch; Kevan L. Hartshorn Collectins are multimeric host defence lectins with trimeric CRDs (carbohydrate-recognition domains) and collagen and N-terminal domains that form higher...

... form {C-16 or CLL-I [16 kDa; chicken lactose lectin, later named CG-16 (chicken galectin-16)]} and the embryonic skin/intestine form (CLL-II or C-14; later named CG-14). In the present study, we report on the cloning and expression of a third prototype CG. It has deceptively similar electrophoretic...

... of two-domain GNA-related lectins. Glycan microarray analyses, frontal affinity chromatography and surface plasmon resonance measurements demonstrated that the two-domain GNA-related lectins acquired a marked diversity in carbohydrate-binding specificity that strikingly contrasts the canonical exclusive...

...Tun-Hsun Kuo; Shiao-Cheng Chuang; Sing-Yang Chang; Po-Huang Liang TPL ( Tachypleus plasma lectin)-1 was purified by using a Sepharose column and TPL-2 was purified from an LPS–Sepharose (LPS coupled to Sepharose matrix) affinity column, as described previously [Chiou, Chen, Y.-W., Chen, S.-C., Chao...

...Tanuja Singh; June H. Wu; Willy J. Peumans; Pierre Rougé; Els J. M. Van Damme; Richard A. Alvarez; Ola Blixt; Albert M. Wu Preliminary studies indicated that the potent insecticidal lectin, Gleheda, from the leaves of Glechoma hederacea (ground ivy) preferentially agglutinates human erythrocytes...

... binding to laminin-1 and carbohydrate-dependent mAbs (monoclonal antibodies) IIH6 and VIA4 1 . We digested highly purified skeletal muscle α-dystroglycan with an array of linkage-specific endo- and exoglycosidases, which were verified for action on α-dystroglycan by loss/gain of reactivity for lectins...

.... In the present study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas aeruginosa , by milk oligosaccharides. Two fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with dissociation constants of 2.2×10 −7 M...

... module (CBM) cellulose-binding domain lectin protein–carbohydrate recognition protein structure The non-catalytic polysaccharide-recognizing modules of glycoside hydrolases were originally defined as CBDs (cellulose-binding domains), because the first examples of these protein domains bound...

...Hiroaki TATENO; Harry C. WINTER; Irwin J. GOLDSTEIN Lectin from the mushroom Polyporus squamosus (PSL) has a unique carbohydrate-binding specificity for sialylated glycoconjugates containing Neu5Acα2,6Galβ1,4Glc/GlcNAc trisaccharide sequences of asparagine-linked glycoproteins. In the present study...

...Willy J. PEUMANS; Pierre ROUGÉ; Els J. M. van DAMME A cDNA encoding a putative lectin expressed in tomato leaves was identified and analysed. The lectin consists of two homologous chitin-binding modules interconnected by a short proline-rich domain containing a single Ser[Pro] n repetitive motif...

... investigated using our collection of glycans by enzyme-linked lectinosorbent assay and lectin–glycan inhibition assay. The results indicate that polyvalent Galβ1-related glycotopes, GalNAcα1-Ser/Thr ( Tn ), and their cryptoforms, are the most potent factor for ABA binding. They were up to 5.5×10 5 and 4.7×10 6...

.... 2 To whom correspondence should be addressed (e-mail [email protected] ). 12 11 2001 27 2 2002 22 4 2002 The Biochemical Society, London ©2002 2002 lectin lipoarabinomannan tuberculosis Abbreviations used: AG, mycobacterial arabinogalactan; BALF...

... of the closely related and naturally occurring loss-of-activity mutant called S. nigra agglutinin lectin-related protein. In the first mutant SNA-If-M1 the high-affinity site 2 of SNA-If was disrupted by replacing the presumed critical residue Asp 231 with Glu 231 . In the double mutant SNA-If-M2, site 1 of SNA...

...Albert M. WU; June H. WU; Ming-Sung TSAI; Herbert KALTNER; Hans-J. GABIUS Owing to the expression of more than one type of galectin in animal tissues, the delineation of the functions of individual members of this lectin family requires the precise definition of their carbohydrate specificities...

.... This suggests that the inhibitory effect of AGP on the infection of human primary macrophages by R5 HIV-1 may be related to specific binding of AGP to a macrophage membrane lectin or lectin-like component and to CCR5. 1 To whom correspondence should be addressed (e-mail [email protected]...

...James MUNDAY; Sheena KERR; Jian NI; Ann L. CORNISH; Jiquan Q. ZHANG; Gavin NICOLL; Helen FLOYD; Marie-Genevieve MATTEI; Paul MOORE; Ding LIU; Paul R. CROCKER Here we characterize Siglec-10 as a new member of the Siglec family of sialic acid-binding Ig-like lectins. A full-length cDNA was isolated...

...Snehasikta SWARNAKAR; Rengasamy ASOKAN; James P. QUIGLEY; Peter B. ARMSTRONG The mediator of haemolysis in the plasma of the horseshoe crab, Limulus polyphemus , is limulin, a sialic acid-binding lectin. The haemolytic activity of limulin is inhibited by thiol ester-reacted forms of Limulus α 2...