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Keywords: lectin
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Biochem J (2020) 477 (17): 3147–3165.
Published: 04 September 2020
.... apoptosis lectin molecular dynamics NMR relaxation NMR spectroscopy site-directed mutagenesis Screening for up- or down-regulation of expression of distinct genes upon changing a specific parameter is a robust approach to identify key aspects of the cascade leading to a triggered cellular...
Includes: Supplementary data
Biochem J (2019) 476 (18): 2623–2655.
Published: 24 September 2019
... have emerged to account for their specific biological meaning. Acting as ‘readers’ of glycan-encoded information, carbohydrate-specific receptors (lectins) are a means to turn the glycans’ potential to serve as signals into a multitude of (patho)physiologically relevant responses. Once the far-reaching...
Includes: Supplementary data
Biochem J (2019) 476 (1): 101–113.
Published: 11 January 2019
...Antonio Eufrásio Vieira Neto; Felipe Domingos de Sousa; Humberto D'Muniz Pereira; Frederico Bruno Mendes Batista Moreno; Marcos Roberto Lourenzoni; Thalles Barbosa Grangeiro; Ana Cristina de Oliveira Monteiro Moreira; Renato de Azevedo Moreira Frutalin (FTL) is a multiple-binding lectin belonging...
Includes: Supplementary data
Biochem J (2012) 446 (1): 149–157.
Published: 27 July 2012
.... chitinase chitinase-like proteins glycan glycan array glycobiology protein structure lectin X-ray crystallography The co-ordinates and structure factors have been deposited in the PDB under accession code 4AY1. 1 These authors contributed equally to this work. 2 To whom...
Biochem J (2009) 420 (2): 239–247.
Published: 13 May 2009
... © The Authors Journal compilation © 2009 Biochemical Society 2009 carbohydrate hydration lectin molecular recognition structural energetics surface area model Heat capacity effects have long been recognized as key to understanding the molecular principles that underpin non-covalent...
Biochem J (2008) 412 (2): 323–329.
Published: 14 May 2008
...Tesfaldet Tecle; Mitchell R. White; Grith Sorensen; Donald Gantz; Nilgun Kacak; Uffe Holmskov; Kelly Smith; Erika C. Crouch; Kevan L. Hartshorn Collectins are multimeric host defence lectins with trimeric CRDs (carbohydrate-recognition domains) and collagen and N-terminal domains that form higher...
Biochem J (2008) 409 (2): 591–599.
Published: 21 December 2007
... form {C-16 or CLL-I [16 kDa; chicken lactose lectin, later named CG-16 (chicken galectin-16)]} and the embryonic skin/intestine form (CLL-II or C-14; later named CG-14). In the present study, we report on the cloning and expression of a third prototype CG. It has deceptively similar electrophoretic...
Includes: Supplementary data
Biochem J (2007) 404 (1): 51–61.
Published: 26 April 2007
... of two-domain GNA-related lectins. Glycan microarray analyses, frontal affinity chromatography and surface plasmon resonance measurements demonstrated that the two-domain GNA-related lectins acquired a marked diversity in carbohydrate-binding specificity that strikingly contrasts the canonical...
Biochem J (2006) 393 (3): 757–766.
Published: 13 January 2006
...Tun-Hsun Kuo; Shiao-Cheng Chuang; Sing-Yang Chang; Po-Huang Liang TPL ( Tachypleus plasma lectin)-1 was purified by using a Sepharose column and TPL-2 was purified from an LPS–Sepharose (LPS coupled to Sepharose matrix) affinity column, as described previously [Chiou, Chen, Y.-W., Chen, S.-C., Chao...
Biochem J (2006) 393 (1): 331–341.
Published: 12 December 2005
...Tanuja Singh; June H. Wu; Willy J. Peumans; Pierre Rougé; Els J. M. Van Damme; Richard A. Alvarez; Ola Blixt; Albert M. Wu Preliminary studies indicated that the potent insecticidal lectin, Gleheda, from the leaves of Glechoma hederacea (ground ivy) preferentially agglutinates human erythrocytes...
Includes: Supplementary data
Biochem J (2005) 390 (1): 303–309.
Published: 09 August 2005
... binding to laminin-1 and carbohydrate-dependent mAbs (monoclonal antibodies) IIH6 and VIA4 1 . We digested highly purified skeletal muscle α-dystroglycan with an array of linkage-specific endo- and exoglycosidases, which were verified for action on α-dystroglycan by loss/gain of reactivity for lectins...
Includes: Supplementary data
Biochem J (2005) 389 (2): 325–332.
Published: 05 July 2005
... present study, we demonstrate the specific blocking of PA-IIL, a fucose-binding lectin of the human pathogen Pseudomonas aeruginosa , by milk oligosaccharides. Two fucosylated epitopes, Lewis a and 3-fucosyl-lactose (Lewis x glucose analogue) bind to the lectin with dissociation constants of 2.2×10 −7 M...
Biochem J (2004) 382 (3): 769–781.
Published: 07 September 2004
... module (CBM) cellulose-binding domain lectin protein–carbohydrate recognition protein structure The non-catalytic polysaccharide-recognizing modules of glycoside hydrolases were originally defined as CBDs (cellulose-binding domains), because the first examples of these protein domains bound...
Biochem J (2004) 382 (2): 667–675.
Published: 24 August 2004
...Hiroaki TATENO; Harry C. WINTER; Irwin J. GOLDSTEIN Lectin from the mushroom Polyporus squamosus (PSL) has a unique carbohydrate-binding specificity for sialylated glycoconjugates containing Neu5Acα2,6Galβ1,4Glc/GlcNAc trisaccharide sequences of asparagine-linked glycoproteins. In the present study...
Biochem J (2003) 376 (3): 717–724.
Published: 15 December 2003
...Willy J. PEUMANS; Pierre ROUGÉ; Els J. M. van DAMME A cDNA encoding a putative lectin expressed in tomato leaves was identified and analysed. The lectin consists of two homologous chitin-binding modules interconnected by a short proline-rich domain containing a single Ser[Pro] n repetitive motif...
Biochem J (2003) 371 (2): 311–320.
Published: 15 April 2003
... investigated using our collection of glycans by enzyme-linked lectinosorbent assay and lectin–glycan inhibition assay. The results indicate that polyvalent Galβ1-related glycotopes, GalNAcα1-Ser/Thr ( Tn ), and their cryptoforms, are the most potent factor for ABA binding. They were up to 5.5×10 5 and 4.7×10 6...
Biochem J (2002) 365 (1): 89–97.
Published: 01 July 2002
.... 2 To whom correspondence should be addressed (e-mail Michel.Riviere@ipbs.fr ). 12 11 2001 27 2 2002 22 4 2002 The Biochemical Society, London ©2002 2002 lectin lipoarabinomannan tuberculosis Abbreviations used: AG, mycobacterial arabinogalactan; BALF...
Biochem J (2002) 364 (2): 587–592.
Published: 01 June 2002
... the closely related and naturally occurring loss-of-activity mutant called S. nigra agglutinin lectin-related protein. In the first mutant SNA-If-M1 the high-affinity site 2 of SNA-If was disrupted by replacing the presumed critical residue Asp 231 with Glu 231 . In the double mutant SNA-If-M2, site 1...
Biochem J (2001) 358 (3): 529–538.
Published: 10 September 2001
...Albert M. WU; June H. WU; Ming-Sung TSAI; Herbert KALTNER; Hans-J. GABIUS Owing to the expression of more than one type of galectin in animal tissues, the delineation of the functions of individual members of this lectin family requires the precise definition of their carbohydrate specificities...
Biochem J (2001) 356 (1): 121–128.
Published: 08 May 2001
... binding of AGP to MDM. This suggests that the inhibitory effect of AGP on the infection of human primary macrophages by R5 HIV-1 may be related to specific binding of AGP to a macrophage membrane lectin or lectin-like component and to CCR5. 1 To whom correspondence should be addressed (e-mail...
Biochem J (2001) 355 (2): 489–497.
Published: 06 April 2001
...James MUNDAY; Sheena KERR; Jian NI; Ann L. CORNISH; Jiquan Q. ZHANG; Gavin NICOLL; Helen FLOYD; Marie-Genevieve MATTEI; Paul MOORE; Ding LIU; Paul R. CROCKER Here we characterize Siglec-10 as a new member of the Siglec family of sialic acid-binding Ig-like lectins. A full-length cDNA was isolated...
Biochem J (2000) 347 (3): 679–685.
Published: 25 April 2000
...Snehasikta SWARNAKAR; Rengasamy ASOKAN; James P. QUIGLEY; Peter B. ARMSTRONG The mediator of haemolysis in the plasma of the horseshoe crab, Limulus polyphemus , is limulin, a sialic acid-binding lectin. The haemolytic activity of limulin is inhibited by thiol ester-reacted forms of Limulus α 2...