... to dimers; and a tightly bound sulfoquinovosyl diacylglycerol (SQDG) lipid that interacts with two PufX arginine residues. This lipid lies at the dimer interface alongside weak density for a second molecule, previously proposed to be an ornithine lipid. In this work we have generated strains of Rba...

... by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY-NC-ND) . contact sites lipids membrane proteins phosphorylation plasma membrane protein kinases For a eukaryotic cell to proliferate, it must be able...

... to power transport. Here, using the model DASS co-transporter, VcINDY from Vibrio cholerae , we have examined the interplay of the three major interactions that occur during transport: the coupling ion, the substrate, and the lipid environment. Using a series of high-throughput thermostability-based...

... greatly expanded these last twenty years. Much progress has been made in understanding the roles and regulations of class I PI3Ks which produce the lipid second messenger phosphatidylinositol 3,4,5 trisphosphate (PtdIns(3,4,5)P 3 ). Selective pharmacological inhibitors and genetic approaches have allowed...

... and infectivity is largely due to its unique waxy cell envelope, 40% of which comprises complex lipids. Therefore, an understanding of the structure and function of the cell wall lipids is of huge indirect clinical significance. This review provides a synopsis of the cell envelope and the major lipids contained...

... and binds long-chain polyunsaturated fatty acids in its groove formed from the α1 and α2 domains. To identify the molecular basis of its lipid-binding function, we determined the first crystal structure at 2.49 Å resolution for fatty acid-bound ZAG, where the ligand was the fluorescent 11-(dansylamino...

..., PlpD-like proteins are present in many bacterial species. We characterized the enzymatic activity, specific lipid binding and oligomeric status of PlpD homologs from Aeromonas hydrophila (a fish pathogen), Burkholderia pseudomallei (a human pathogen) and Ralstonia solanacearum (a plant pathogen...

... metabolism lipids membrane transporter Abbreviations used: BMIPP, 15-( p -iodophenyl)-3( R , S )-methyl pentadecanoic acid; FA, long-chain fatty acids; FABP, fatty acid binding protein; FABP c , cytoplasmic FABP; H-FABP c , heart-type FABP c ; FABP PM , plasma membrane FABP; FAT, fatty acid...

...% was PtdIns3 P and the rest was PtdIns4 P . In seedlings of Vicia faba and suspension-cultured tomato cells, the level of PtdIns5 P was about 18%, indicating that PtdIns5 P is a general plant lipid that represents a significant proportion of the PtdIns P pool. Activating phospholipase C (PLC) signalling...

... and lipids, all classes of biomolecules can be analysed, and much excellent work has been done in the fields of carbohydrate and nucleic acid biochemistry. Biochem. J. (2001) 355, 545 561 (Printed in Great Britain) 545 REVIEW ARTICLE Electrospray and tandem mass spectrometry in biochemistry William J...