... the contribution of the repression of lipogenesis by AMPK in these processes, we compared the effect of pharmacological inhibition of acetyl-CoA carboxylase (ACC). We show that AMPK activation inhibits adipogenesis in a time- and concentration-dependent manner. Transient AMPK activation during adipogenic...

... stress hepatic physiology insulin resistance insulin signalling lipogenesis sterol regulatory element-binding proteins In 2002, we wrote a review in the Biochemical Journal on the importance of the transcription factor Sterol Regulatory Element Binding Protein-1c (SREBP-1c...

... in multiple cell types, including hepatocytes, skeletal muscle cells and adipocytes. We observed SBI-0206965 dose dependently attenuated AMPK activator (991)-stimulated ACC phosphorylation and inhibition of lipogenesis in hepatocytes. SBI-0206965 (≥25 μM) modestly inhibited AMPK signalling in C2C12 myotubes...

...Nusrat Hussain; Sheng-Ju Chuang; Manuel Johanns; Didier Vertommen; Gregory R. Steinberg; Bruce E. Kemp; Mark H. Rider We investigated acute effects of two allosteric protein kinase B (PKB) inhibitors, MK-2206 and Akti-1/2, on insulin-stimulated lipogenesis in rat epididymal adipocytes incubated...

... fatty acid synthesis diacylglycerol acyltransferase heart hypertriglyceridaemia intestine lipogenesis liver muscle TAGs (triacylglycerols; also known as triglycerides) perform multiple functions in mammalian cells and in the traffic of substrates between tissues. They are a major source...

...Guohui Wang; Xuebao Zhang; Jin-Sook Lee; Xiaogang Wang; Zeng-Quan Yang; Kezhong Zhang Increased de novo lipogenesis is a hallmark of aggressive cancers. Lipid droplets, the major form of cytosolic lipid storage, have been implicated in cancer cell proliferation and tumorigenesis. Recently, we...

... hepatocytes (5%) and for lipogenesis in rat adipocytes (4%). In contrast, the INS-B analogue, which has an increased affinity for human IR-B, had higher relative potencies (compared with HI) for inducing glycogen accumulation (75%) and lipogenesis (130%) than for affecting muscle (45%). For the same blood...

.../EBPα (CCAAT/enhancer-binding protein α) and accelerate lipogenesis accompanied with increased GLUT (glucose transporter) 1 and GLUT4. CREBL2 knockdown restrained adipogenic conversion and lipogenesis. Additionally, depletion of CREB could completely block the effects of overexpressed CREBL2, whereas...

... of lipogenesis stimulated by glucose and insulin. Moreover, CPT1A sensitivity to malonyl-CoA inhibition varies markedly depending on the physiopathological state of the animal. In the present study, we asked whether an increase in CPT1A activity solely or in association with a decreased malonyl-CoA sensitivity...

... and fatty acid synthesis by binding to SREs or to palindromic sequences called E-boxes within their promoter regions [ 15 , 16 ]. Moreover, it has been reported that PUFA regulate the nuclear abundance of SREBP-1 [ 17 – 19 ]. Given the importance of CiC as a key step in lipogenesis and due to the poor...

..., which is also induced in lactation. This indicates that SREBP-1 is a major developmental regulator of the programme of lipid synthesis de novo in the lactating mammary gland. Key words: basic helix loop helix, chromatin immunoprecipita- tion, lactation, lipogenesis, STAT5. INTRODUCTION ACC (acetyl-CoA...

...Mary C. SUGDEN; Karen BULMER; Geoffrey F. GIBBONS; Brian L. KNIGHT; Mark J. HOLNESS The aim of the present study was to determine whether peroxisome-proliferator-activated receptor-α (PPARα) deficiency disrupts the normal regulation of triacylglycerol (TAG) accumulation, hepatic lipogenesis...

... 7 2001 22 10 2001 13 11 2001 The Biochemical Society, London ©2002 2002 enhanceosome fatty acid synthesis lipogenesis nuclear T3 receptor orphan receptor Abbreviations used: CEH, chick embryo hepatocytes; CEF, chick embryo fibroblasts; CAT, chloramphenicol...

.... 9 3 2001 2 7 2001 7 8 2001 The Biochemical Society, London ©2001 2001 adipose tissue HepG2 cells insulin signalling lipogenesis preadipocyte Abbreviations used: ACC-α, acetyl-CoA carboxylase-α; BAC, bacterial artificial chromosome; bHLH, basic helix-loop-helix; C...

[email protected] ). 20 3 2001 17 5 2001 11 6 2001 The Biochemical Society, London ©2001 2001 glucose glycolysis hepatocytes lipogenesis Abbreviations used: ACC, acetyl-CoA carboxylase; COUP-TF1, chicken ovalbumin upstream promoter-transcription factor 1; hCOUP-TF1...

... transcription glucose DNA regulatory sequences lipogenesis reporter gene Abbreviations used: Ang II, angiotensin II; ADD1, adipocyte determination and differentiation factor 1; CAT, chloramphenicol acetyltransferase; E box, insulin-responsive region; FAS, fatty acid synthase; IRE, insulin...

... cells were exposed to lipolytic and anti-lipogenic agents, forskolin and isoproterenol. Taken together, our data show that Id products are functionally involved in modulating ADD1/SREBP-1c transcriptional activity, and thus lipogenesis in adipocytes. 1 To whom correspondence should be addressed...

... of antioxidants must be interpretated cautiously and (ii) that in vivo , the inhibitory effect of PUFA on lipogenesis-related genes could be indirect through hormonal or metabolic changes or that their effect on gene expression is somehow linked to peroxidative mechanisms. 1 To whom correspondence should...

... in gluconeogenesis and lipogenesis, in the biosynthesis of neurotransmitter substances, and in glucose-induced insulin secretion by pancreatic islets. The reaction catalysed by PC and the physical properties of the enzyme have been studied extensively. Although no high-resolution three-dimensional structure has yet...