...Alexander Yang; Emilio P. Mottillo Fatty acids (FAs) are stored safely in the form of triacylglycerol (TAG) in lipid droplet (LD) organelles by professional storage cells called adipocytes. These lipids are mobilized during adipocyte lipolysis, the fundamental process of hydrolyzing TAG to FAs...

... insulin and β-adrenergic stimulation in concert controls lipolysis in mature subcutaneous adipocytes obtained from non-diabetic and, in parallel, type 2 diabetic women. We report that, and show how, the anti-lipolytic effect of insulin can be fully explained by protein kinase B (PKB/Akt)-dependent...

... by an increased AMP/ATP ratio. Adrenaline incubation of isolated adipocytes also increased the AMP/ATP ratio and AMPK activities, an effect blocked by propranolol. Adrenaline incubation increased lipolysis in isolated adipocytes, and Compound C, an AMPK inhibitor, attenuated this effect. Finally, a potential role...

...Enhui WEI; Richard LEHNER; Dennis E. VANCE TGH (triacylglycerol hydrolase) catalyses the lipolysis of intracellular stored triacylglycerol. To explore the mechanisms that regulate TGH expression in adipose tissue, we studied the expression of TGH during the differentiation of 3T3-L1 adipocytes. TGH...

... metabolism hormone-sensitive lipase lipolysis macrophage perilipin Abbreviations used: ACAT, acyl-CoA:cholesterol acyltransferase; ADRP, adipocyte differentiation-related protein; ANP, atrial natriuretic peptide; DFP, di-isopropyl fluorophosphate; ERK, extracellular-signal-regulated kinase; FABP...

...-kinase (PFK-2) activity was eliminated when the liver isoform of PFK-2 was shown to be absent from β-cells. The possibility that cAMP enhanced pulsatile secretion, at least in part, by stimulating lipolysis was supported by the observations that added oleate had a similar effect on secretion...

... simultaneous recruitment of all major extra- and intra-muscular energy stores. 1 To whom correspondence should be addressed (e-mail [email protected] ). 10 2 2000 28 6 2000 3 8 2000 The Biochemical Society, London © 2000 2000 exercise glycogen phosphorylase lipolysis...

... the beneficial effect of diminishing fat-cell number and lipid content. Key words: differentiation, lipolysis, triacylglycerol storage. than 2-fold higher than those in older ones (3-months old; W. Guo and B. E. Corkey, unpublished work). This is most probably due to storage of MCFA acquired from milk...

... days after the initiation of differentiation of 3T3-L1 preadipocytes. Treatment of 3T3-L1 adipocytes with 10 ng/ml TNF-α for 24 h produced a maximal (50%) decrease in PDE3B activity, protein and mRNA, which was well correlated with both activation of protein kinase A (PKA) and stimulation of lipolysis...

..., lipolysis, okadaic acid. growth-hormone signalling in adipocytes [15]. The effects of insulin, growth hormone, IGF-I and IL-4 on PDE3B have been suggested to be important in antagonizing biological responses induced by cAMP, such as lipolysis in adipocytes [6,15,16], glycogenolysis in the liver [17...

.... 1 To whom correspondence should be addressed ( [email protected] ). 29 1 1999 13 4 1999 7 5 1999 The Biochemical Society, London © 1999 1999 cytosol endoplasmic reticulum lipid synthesis lipolysis secretion Biochem. J. (1999) 341, 453 460 (Printed in Great...