... for storage, segmentation, analysis, and statistical parametrization of live cell imaging datasets. A great deal of relevant biological information is lost when phenomena are observed with techniques that measure static snapshots of cellular processes without actively tracking them over time and space. Many...

... 2021 AML12 cells iron metabolism lipid metabolism live-cell imaging liver synchrotron infrared spectroscopy The objective of this study was to investigate the extent to which iron overload would perturb lipid metabolism leading to a steatosis phenotype. To achieve this, we utilised...

... with the second transmembrane segments being in spatial proximity [ 10 ]. cell junctions claudin claudin strand model FRET live-cell imaging tight junction architecture The TJ (tight junction) regulates the paracellular passage of solutes and water in a size- and charge-selective manner...

... tested this hypothesis by the use of cells, intact mitochondria and live-cell imaging of acute tissue slices isolated from mice in which Nrf2 is either genetically deleted or constitutively activated, and comparing them with their WT counterparts. WT, Nrf2-KO and Keap1-KD C57BL/6 mice were...

...Joshua Z. Rappoport Investigations into the mechanisms which regulate entry of integral membrane proteins, and associated ligands, into the cell through vesicular carriers (endocytosis) have greatly benefited from the application of live-cell imaging. Several excellent recent reviews have detailed...

.... In addition, nuclear apoE localization in ovarian cancer was recently shown to be correlated with patient survival. In order to better understand the factors associated with apoE nuclear localization, we examined intracellular apoE trafficking using live-cell imaging of CHO (Chinese-hamster ovary) cells...