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Articles

Resveratrol promotes lysosomal function via ER calcium-dependent TFEB activation to ameliorate lipid accumulation

Rong Shao, Jiahui Shi, Kaili Du, Na Wang, Weijie Cai, Siyu Liu, Zongxian Ding, Yihan Wang, Dan Li

Biochem J (2021) 478 (5): 1159–1173.

https://doi.org/10.1042/BCJ20200676

Published: 12 March 2021

... reticulum (ER)- Ca 2+ signaling that activates transcriptional factor EB (TFEB), a master transcriptional regulator of autophagic and lysosomal biogenesis. Moreover, RSV activates protein phosphatase 2A (PP2A), which binds and dephosphorylates TFEB, promoting its nuclear translocation and the expression...

View article titled, Resveratrol promotes lysosomal function via ER calcium-dependent TFEB activation to ameliorate lipid accumulation

Articles

Establishment and characterization of Neu1-knockout zebrafish and its abnormal clinical phenotypes

Keiji Okada, Ryo Takase, Yurie Hamaoka, Akinobu Honda, Asami Ikeda, Yoichiro Hokazono, Yutaro Maeda, Oki Hayasaka, Tomonari Kotani, Masaharu Komatsu, Kazuhiro Shiozaki

Biochem J (2020) 477 (15): 2841–2857.

https://doi.org/10.1042/BCJ20200348

Published: 12 August 2020

... metastasis, and diabetes through lysosomal catabolism and desialylation of glycoproteins at the plasma membrane. Various animal models have been established to further explore the functions of vertebrate Neu1. The present study focused on zebrafish ( Danio rerio ) belonging to Cypriniformes...

View article titled, Establishment and characterization of Neu1-knockout zebrafish and its abnormal clinical phenotypes

Includes: Supplementary data

Articles

PAS domain-containing phosphoglycerate kinase deficiency in Leishmania major results in increased autophagosome formation and cell death

Ayan Adhikari, Saroj Biswas, Aditi Mukherjee, Sumit Das, Subrata Adak

Biochem J (2019) 476 (8): 1303–1321.

https://doi.org/10.1042/BCJ20190041

Published: 30 April 2019

... stimulate PGK activity. Localization studies demonstrate that the protein is highly enriched in the glycosome and its presence can also be seen in the lysosome. Gene knockout, overexpression and complement studies suggest that LmPAS-PGK plays a fundamental role in cell survival through autophagy...

View article titled, PAS domain-containing phosphoglycerate kinase deficiency in Leishmania major results in increased autophagosome formation and cell death

Includes: Supplementary data

Articles

Proteasome regulates turnover of toxic human amylin in pancreatic cells

Sanghamitra Singh, Saurabh Trikha, Anjali Sarkar, Aleksandar M. Jeremic

Biochem J (2016) 473 (17): 2655–2670.

https://doi.org/10.1042/BCJ20160026

Published: 30 August 2016

... followed by its translocation into nucleus, and to a lesser extent lysosomes, keeping the net cytosolic amylin content low. An increase in hA accumulation in the nucleus of pancreatic cells correlated with its cytotoxicity, suggesting that its excessive accumulation in the nucleus is detrimental. hA...

View article titled, Proteasome regulates turnover of toxic human amylin in pancreatic cells

Includes: Supplementary data

Articles

A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I

Michael D.J. Parkinson, Siân C. Piper, Nicholas A. Bright, Jennifer L. Evans, Jessica M. Boname, Katherine Bowers, Paul J. Lehner, J. Paul Luzio

Biochem J (2015) 471 (1): 79–88.

https://doi.org/10.1042/BJ20150336

Published: 21 September 2015

... ligase that promotes Lys 63 -linked polyubiquitination of MHC class I, providing the signal for clathrin-mediated endocytosis. Endocytosis is followed by sorting into the intralumenal vesicles (ILVs) of multivesicular bodies (MVBs) and eventual delivery to lysosomes. The sorting of MHC class I into MVBs...

View article titled, A non-canonical ESCRT pathway, including histidine domain phosphotyrosine phosphatase (HD-PTP), is used for down-regulation of virally ubiquitinated MHC class I

Articles

Role of the N-terminal transmembrane domain in the endo-lysosomal targeting and function of the human ABCB6 protein

Katalin Kiss, Nora Kucsma, Anna Brozik, Gabor E. Tusnady, Ptissam Bergam, Guillaume van Niel, Gergely Szakacs

Biochem J (2015) 467 (1): 127–139.

https://doi.org/10.1042/BJ20141085

Published: 20 March 2015

... localization of ABCB6 has been a matter of debate, as it has been suggested to reside in the mitochondria and the endo-lysosomal system. Using a variety of imaging modalities, including confocal microscopy and EM, we confirm the endo-lysosomal localization of ABCB6 and show that the protein is internalized...

View article titled, Role of the N-terminal transmembrane domain in the endo-lysosomal targeting and function of the human ABCB6 protein

Articles

PI4KIIα phosphorylation by GSK3 directs vesicular trafficking to lysosomes

James W. Robinson, Iryna Leshchyns’ka, Hovik Farghaian, William E. Hughes, Vladimir Sytnyk, Graham G. Neely, Adam R. Cole

Biochem J (2014) 464 (1): 145–156.

https://doi.org/10.1042/BJ20140497

Published: 23 October 2014

... phosphorylates two distinct sites in the N-terminus of PI4KIIα (Ser 5 and Ser 47 ), promoting binding to the adaptor protein 3 (AP-3) complex for trafficking to the lysosome to be degraded. Blocking phosphorylation reduces trafficking to the lysosome, stabilizing PI4KIIα and its cargo proteins for redistribution...

View article titled, PI4KIIα phosphorylation by GSK3 directs vesicular trafficking to lysosomes

Articles

The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP

Dev Churamani, Robert Hooper, Taufiq Rahman, Eugen Brailoiu, Sandip Patel

Biochem J (2013) 453 (1): 147–151.

https://doi.org/10.1042/BJ20130474

Published: 13 June 2013

... These authors contributed equally to this work. 2 To whom correspondence should be addressed (email [email protected] ). 12 4 2013 1 5 2013 2 5 2013 2 5 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 calcium endosome lysosome nicotinic acid...

View article titled, The N-terminal region of two-pore channel 1 regulates trafficking and activation by NAADP

Articles

The mannose 6-phosphate-binding sites of M6P/IGF2R determine its capacity to suppress matrix invasion by squamous cell carcinoma cells

Olivia C. Probst, Evren Karayel, Nicole Schida, Elisabeth Nimmerfall, Elisabeth Hehenberger, Verena Puxbaum, Lukas Mach

Biochem J (2013) 451 (1): 91–99.

https://doi.org/10.1042/BJ20121422

Published: 14 March 2013

... is sufficient to impair all cellular functions of the receptor tested. These findings highlight that the interaction between M6P/IGF2R and M6P-modified ligands is not only important for intracellular accumulation of lysosomal enzymes and formation of dense lysosomes, but is also crucial for the ability...

View article titled, The mannose 6-phosphate-binding sites of M6P/IGF2R determine its capacity to suppress matrix invasion by squamous cell carcinoma cells

Includes: Supplementary data

Articles

Generation and characterization of a lysosomally targeted, genetically encoded Ca 2+ -sensor

Hannah V. McCue, Joanna D. Wardyn, Robert D. Burgoyne, Lee P. Haynes

Biochem J (2013) 449 (2): 449–457.

https://doi.org/10.1042/BJ20120898

Published: 14 December 2012

... (endoplasmic reticulum) or through the opening of Ca 2+ -permeable channels in the plasma membrane and influx of extracellular Ca 2+ . Late endocytic pathway compartments including late-endosomes and lysosomes have recently been observed to sequester Ca 2+ to levels comparable with those found within the ER...

View article titled, Generation and characterization of a lysosomally targeted, genetically encoded Ca 2+ -sensor

Includes: Multimedia, Supplementary data

Articles

Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells

Blanka Halamoda Kenzaoui, Catherine Chapuis Bernasconi, Seher Guney-Ayra, Lucienne Juillerat-Jeanneret

Biochem J (2012) 441 (3): 813–821.

https://doi.org/10.1042/BJ20111252

Published: 16 January 2012

...; however, the extent of their intracellular uptake is dependent on the characteristics of the NPs. After their uptake by human brain-derived endothelial cells NPs are transported into the lysosomes of these cells, where they enhance the activation of lysosomal proteases. In brain-derived endothelial cells...

View article titled, Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells

Articles

Visualization of co-localization in Aβ42-administered neuroblastoma cells reveals lysosome damage and autophagosome accumulation related to cell death

Violetta Soura, Maris Stewart-Parker, Thomas L. Williams, Arjuna Ratnayaka, Joe Atherton, Kirsti Gorringe, Jack Tuffin, Elisabeth Darwent, Roma Rambaran, William Klein, Pascale Lacor, Kevin Staras, Julian Thorpe, Louise C. Serpell

Biochem J (2012) 441 (2): 579–590.

https://doi.org/10.1042/BJ20110749

Published: 21 December 2011

... enters the cells prior to cell death. Immunoconfocal and immunogold electron microscopy reveal the path of the Aβ42 with time through the endosomal system and shows that it accumulates in lysosomes. A 24 h incubation with Aβ results in cells that have damaged lysosomes showing signs of enzyme leakage...

View article titled, Visualization of co-localization in Aβ42-administered neuroblastoma cells reveals lysosome damage and autophagosome accumulation related to cell death

Includes: Supplementary data

Articles

Proteins containing oxidized amino acids induce apoptosis in human monocytes

Rachael A. Dunlop, Ulf T. Brunk, Kenneth J. Rodgers

Biochem J (2011) 435 (1): 207–216.

https://doi.org/10.1042/BJ20100682

Published: 15 March 2011

.../ceroid-like bodies with a lysosomal-type distribution and up-regulate the transcription and translation of proteolytic lysosomal enzymes in cultured J774 mouse macrophages. Given the recently identified role of lysosomes in the induction of apoptosis, we have extended our studies to explore a role...

View article titled, Proteins containing oxidized amino acids induce apoptosis in human monocytes

Includes: Supplementary data

Articles

Two dileucine motifs mediate late endosomal/lysosomal targeting of transmembrane protein 192 (TMEM192) and a C-terminal cysteine residue is responsible for disulfide bond formation in TMEM192 homodimers

Jörg Behnke, Eeva-Liisa Eskelinen, Paul Saftig, Bernd Schröder

Biochem J (2011) 434 (2): 219–231.

https://doi.org/10.1042/BJ20101396

Published: 11 February 2011

...Jörg Behnke; Eeva-Liisa Eskelinen; Paul Saftig; Bernd Schröder TMEM192 (transmembrane protein 192) is a novel constituent of late endosomal/lysosomal membranes with four potential transmembrane segments and an unknown function that was initially discovered by organellar proteomics. Subsequently...

View article titled, Two dileucine motifs mediate late endosomal/lysosomal targeting of transmembrane protein 192 (TMEM192) and a C-terminal cysteine residue is responsible for disulfide bond formation in TMEM192 homodimers

Articles

Chelation of lysosomal iron protects against ionizing radiation

Carsten Berndt, Tino Kurz, Markus Selenius, Aristi P. Fernandes, Margareta R. Edgren, Ulf T. Brunk

Biochem J (2010) 432 (2): 295–301.

https://doi.org/10.1042/BJ20100996

Published: 12 November 2010

... 2 O 2 also form and induce oxidative stress with resulting LMP (lysosomal membrane permeabilization) arising from iron-catalysed oxidative events. The latter will contribute significantly to radiation-induced cell death and its degree largely depends on the quantities of lysosomal redox-active iron...

View article titled, Chelation of lysosomal iron protects against ionizing radiation

Articles

Endocytosis of hyaluronidase-1 by the liver

Marie-Christine Gasingirwa, Jacqueline Thirion, Jeannine Mertens-Strijthagen, Simone Wattiaux-De Coninck, Bruno Flamion, Robert Wattiaux, Michel Jadot

Biochem J (2010) 430 (2): 305–313.

https://doi.org/10.1042/BJ20100711

Published: 13 August 2010

...Marie-Christine Gasingirwa; Jacqueline Thirion; Jeannine Mertens-Strijthagen; Simone Wattiaux-De Coninck; Bruno Flamion; Robert Wattiaux; Michel Jadot It has been suggested that intracellular Hyal-1 (hyaluronidase-1), which is considered a lysosomal enzyme, originates via endocytosis of the serum...

View article titled, Endocytosis of hyaluronidase-1 by the liver

Articles

Glial fibrillary acidic protein is elevated in the lysosomal storage disease classical late-infantile neuronal ceroid lipofuscinosis, but is not a component of the storage material

Su Xu, David E. Sleat, Michel Jadot, Peter Lobel

Biochem J (2010) 428 (3): 355–362.

https://doi.org/10.1042/BJ20100128

Published: 27 May 2010

...Su Xu; David E. Sleat; Michel Jadot; Peter Lobel Classical late-infantile neuronal ceroid lipofuscinosis (LINCL) is a fatal neurodegenerative disease of children caused by mutations in TPP1 , the gene encoding the lysosomal protease tripeptidyl peptidase 1. LINCL is characterized by lysosomal...

View article titled, Glial fibrillary acidic protein is elevated in the lysosomal storage disease classical late-infantile neuronal ceroid lipofuscinosis, but is not a component of the storage material

Includes: Supplementary data

Articles

What does the commonly used DCF test for oxidative stress really show?

Markus Karlsson, Tino Kurz, Ulf T. Brunk, Sven E. Nilsson, Christina I. Frennesson

Biochem J (2010) 428 (2): 183–190.

https://doi.org/10.1042/BJ20100208

Published: 13 May 2010

... of H 2 DCF requires the presence of either cytochrome c or of both redox-active transition metals and H 2 O 2 . Redox-active metals exist mainly within lysosomes, whereas cytochrome c resides bound to the outer side of the inner mitochondrial membrane. Following exposure to H 2 DCF-DA, weak...

View article titled, What does the commonly used DCF test for oxidative stress really show?

Articles

Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia

Maria R. Rivero, Cecilia V. Vranych, Mariano Bisbal, Belkys A. Maletto, Andrea S. Ropolo, Maria C. Touz

Biochem J (2010) 428 (1): 33–45.

https://doi.org/10.1042/BJ20100096

Published: 28 April 2010

...Maria R. Rivero; Cecilia V. Vranych; Mariano Bisbal; Belkys A. Maletto; Andrea S. Ropolo; Maria C. Touz The parasite Giardia lamblia possesses PVs (peripheral vacuoles) that function as both endosomes and lysosomes and are implicated in the adaptation, differentiation and survival of the parasite...

View article titled, Adaptor protein 2 regulates receptor-mediated endocytosis and cyst formation in Giardia lamblia

Includes: Supplementary data

Articles

Recruitment of NAADP-sensitive acidic Ca 2+ stores by glutamate

Vinita Pandey, Chia-Chen Chuang, Alexander M. Lewis, Parvinder K. Aley, Eugen Brailoiu, Nae J. Dun, Grant C. Churchill, Sandip Patel

Biochem J (2009) 422 (3): 503–512.

https://doi.org/10.1042/BJ20090194

Published: 27 August 2009

...Vinita Pandey; Chia-Chen Chuang; Alexander M. Lewis; Parvinder K. Aley; Eugen Brailoiu; Nae J. Dun; Grant C. Churchill; Sandip Patel NAADP (nicotinic acid–adenine dinucleotide phosphate) is an unusual second messenger thought to mobilize acidic Ca 2+ stores, such as lysosomes or lysosome-like...

View article titled, Recruitment of NAADP-sensitive acidic Ca 2+ stores by glutamate

Articles

NCU-G1 is a highly glycosylated integral membrane protein of the lysosome

Oliver Schieweck, Markus Damme, Bernd Schröder, Andrej Hasilik, Bernhard Schmidt, Torben Lübke

Biochem J (2009) 422 (1): 83–90.

https://doi.org/10.1042/BJ20090567

Published: 29 July 2009

...Oliver Schieweck; Markus Damme; Bernd Schröder; Andrej Hasilik; Bernhard Schmidt; Torben Lübke Until recently, a modest number of approx. 40 lysosomal membrane proteins had been identified and even fewer were characterized in their function. In a proteomic study, using lysosomal membranes from...

View article titled, NCU-G1 is a highly glycosylated integral membrane protein of the lysosome

Articles

Clioquinol targets zinc to lysosomes in human cancer cells

Haijun Yu, Yunfeng Zhou, Stuart E. Lind, Wei-Qun Ding

Biochem J (2009) 417 (1): 133–139.

https://doi.org/10.1042/BJ20081421

Published: 12 December 2008

... was confirmed using fluorescence microscopy. Intracellular free zinc was found to be concentrated in lysosomes, indicating that lysosomes are the primary target of zinc ionophores. Furthermore, lysosomal integrity was disrupted after addition of clioquinol and zinc to the cells, as shown by redistribution...

View article titled, Clioquinol targets zinc to lysosomes in human cancer cells

Articles

A dileucine signal situated in the C-terminal tail of the lysosomal membrane protein p40 is responsible for its targeting to lysosomes

Marielle Boonen, Roberta Rezende de Castro, Gaëlle Cuvelier, Isabelle Hamer, Michel Jadot

Biochem J (2008) 414 (3): 431–440.

https://doi.org/10.1042/BJ20071626

Published: 27 August 2008

...Marielle Boonen; Roberta Rezende de Castro; Gaëlle Cuvelier; Isabelle Hamer; Michel Jadot Transport of newly synthesized lysosomal membrane proteins from the TGN ( trans -Golgi network) to the lysosomes is due to the presence of specific signals in their cytoplasmic domains that are recognized...

View article titled, A dileucine signal situated in the C-terminal tail of the lysosomal membrane protein p40 is responsible for its targeting to lysosomes

Articles

Mucolipin 1 channel activity is regulated by protein kinase A-mediated phosphorylation

Silvia Vergarajauregui, Ross Oberdick, Kirill Kiselyov, Rosa Puertollano

Biochem J (2008) 410 (2): 417–425.

https://doi.org/10.1042/BJ20070713

Published: 12 February 2008

...Silvia Vergarajauregui; Ross Oberdick; Kirill Kiselyov; Rosa Puertollano Mucolipins constitute a family of cation channels with homology with the transient receptor potential family. Mutations in MCOLN1 (mucolipin 1) have been linked to mucolipidosis type IV, a recessive lysosomal storage disease...

View article titled, Mucolipin 1 channel activity is regulated by protein kinase A-mediated phosphorylation

Includes: Supplementary data

Articles

The impact of specific oxidized amino acids on protein turnover in J774 cells

Rachael A. Dunlop, Roger T. Dean, Kenneth J. Rodgers

Biochem J (2008) 410 (1): 131–140.

https://doi.org/10.1042/BJ20070161

Published: 29 January 2008

... dopa incorporation generated proteins that were inefficiently degraded by cells. Incorporation of higher levels of L -dopa into proteins resulted in an increase in the activity of lysosomal cathepsins, increased autofluorescence and the generation of high-molecular-mass SDS-stable complexes, indicative...

View article titled, The impact of specific oxidized amino acids on protein turnover in J774 cells

Articles

Lysosomes and Fas-mediated liver cell death

Robert Wattiaux, Simone Wattiaux-De Coninck, Jacqueline Thirion, Mańe-Christine Gasingirwa, Michel Jadot

Biochem J (2007) 403 (1): 89–95.

https://doi.org/10.1042/BJ20061738

Published: 13 March 2007

...Robert Wattiaux; Simone Wattiaux-De Coninck; Jacqueline Thirion; Mańe-Christine Gasingirwa; Michel Jadot A number of studies, mostly performed ex vivo , suggest that lysosomes are involved in apoptosis as a result of a release of their cathepsins into the cytosol. These enzymes could...

View article titled, Lysosomes and Fas-mediated liver cell death

Articles

Intracellular localization of p40, a protein identified in a preparation of lysosomal membranes

Marielle Boonen, Isabelle Hamer, Muriel Boussac, Anne-Françoise Delsaute, Bruno Flamion, Jérôme Garin, Michel Jadot

Biochem J (2006) 395 (1): 39–47.

https://doi.org/10.1042/BJ20051647

Published: 15 March 2006

...Marielle Boonen; Isabelle Hamer; Muriel Boussac; Anne-Françoise Delsaute; Bruno Flamion; Jérôme Garin; Michel Jadot Unlike lysosomal soluble proteins, few lysosomal membrane proteins have been identified. Rat liver lysosomes were purified by centrifugation on a Nycodenz density gradient. The most...

View article titled, Intracellular localization of p40, a protein identified in a preparation of lysosomal membranes

Includes: Supplementary data

Articles

Metallothionein protects against oxidative stress-induced lysosomal destabilization

Sarah K. Baird, Tino Kurz, Ulf T. Brunk

Biochem J (2006) 394 (1): 275–283.

https://doi.org/10.1042/BJ20051143

Published: 27 January 2006

...Sarah K. Baird; Tino Kurz; Ulf T. Brunk The introduction of apo-ferritin or the iron chelator DFO (desferrioxamine) conjugated to starch into the lysosomal compartment protects cells against oxidative stress, lysosomal rupture and ensuing apoptosis/necrosis by binding intralysosomal redox-active...

View article titled, Metallothionein protects against oxidative stress-induced lysosomal destabilization

Articles

Sphingomyelins suppress the targeted disruption of lysosomes/endosomes by the photosensitizer NPe6 during photodynamic therapy

Joseph A. Caruso, Patricia A. Mathieu, John J. Reiners, Jr

Biochem J (2005) 392 (2): 325–334.

https://doi.org/10.1042/BJ20050313

Published: 22 November 2005

...Joseph A. Caruso; Patricia A. Mathieu; John J. Reiners, Jr Recent studies have described a biochemical pathway whereby lysosome disruption and the released proteases initiate the intrinsic apoptotic pathway. Irradiation of murine hepatoma 1c1c7 cells preloaded with the lysosomal photosensitizer...

View article titled, Sphingomyelins suppress the targeted disruption of lysosomes/endosomes by the photosensitizer NPe6 during photodynamic therapy

Articles

Cancer cell injury by cytotoxins from cobra venom is mediated through lysosomal damage

Alexei V. Feofanov, George V. Sharonov, Maria V. Astapova, Dmitriy I. Rodionov, Yuriy N. Utkin, Alexander S. Arseniev

Biochem J (2005) 390 (1): 11–18.

https://doi.org/10.1042/BJ20041892

Published: 09 August 2005

... of their action remains obscure. Using the confocal spectral imaging technique, we show for the first time that cytotoxins from cobra venom penetrate readily into living cancer cells and accumulate markedly in lysosomes. Cytotoxins CT1 and CT2 from Naja oxiana , CT3 from Naja kaouthia and CT1 from Naja haje...

View article titled, Cancer cell injury by cytotoxins from cobra venom is mediated through lysosomal damage

Articles

Radiation-induced cell death: importance of lysosomal destabilization

H. Lennart Persson, Tino Kurz, John W. Eaton, Ulf T. Brunk

Biochem J (2005) 389 (3): 877–884.

https://doi.org/10.1042/BJ20050271

Published: 26 July 2005

... in the cytoplasm due to formation of H 2 O 2 may also be important. Since the major pool of low-mass redox-active intracellular iron seems to reside within lysosomes, arising from the continuous intralysosomal autophagocytotic degradation of ferruginous materials, formation of H 2 O 2 inside and outside...

View article titled, Radiation-induced cell death: importance of lysosomal destabilization

Articles

Role of compartmentalized redox-active iron in hydrogen peroxide-induced DNA damage and apoptosis

Margarita TENOPOULOU, Paschalis-Thomas DOULIAS, Alexandra BARBOUTI, Ulf BRUNK, Dimitrios GALARIS

Biochem J (2005) 387 (3): 703–710.

https://doi.org/10.1042/BJ20041650

Published: 26 April 2005

... a rapid, dose-dependent increase in the ICIP (intracellular calcein-chelatable iron pool). Early destabilization of lysosomal membranes and subsequent nuclear DNA strand breaks were also observed, as evaluated by the Acridine Orange relocation test and the comet assay respectively. Somewhat later...

View article titled, Role of compartmentalized redox-active iron in hydrogen peroxide-induced DNA damage and apoptosis

Articles

Intracellular transport of human lysosomal α-mannosidase and α-mannosidosis-related mutants

Gaute HANSEN, Thomas BERG, Hilde M. F. RIISE STENSLAND, Pirkko HEIKINHEIMO, Helle KLENOW, Gry EVJEN, Øivind NILSSEN, Ole K. TOLLERSRUD

Biochem J (2004) 381 (2): 537–546.

https://doi.org/10.1042/BJ20031499

Published: 06 July 2004

...Gaute HANSEN; Thomas BERG; Hilde M. F. RIISE STENSLAND; Pirkko HEIKINHEIMO; Helle KLENOW; Gry EVJEN; Øivind NILSSEN; Ole K. TOLLERSRUD Human LAMAN (lysosomal α-mannosidase) was synthesized as a 120 kDa precursor in transfected COS cells [African-green-monkey kidney cells], which was partly secreted...

View article titled, Intracellular transport of human lysosomal α-mannosidase and α-mannosidosis-related mutants

Articles

Relocalized redox-active lysosomal iron is an important mediator of oxidative-stress-induced DNA damage

Tino KURZ, Alan LEAKE, Thomas von ZGLINICKI, Ulf T. BRUNK

Biochem J (2004) 378 (3): 1039–1045.

https://doi.org/10.1042/bj20031029

Published: 15 March 2004

...]. Consequently, rupture of secondary lysosomes, as well as subsequent relocation of labile iron to the nucleus, could be an important intermediary step in the generation of oxidative damage to DNA. To test this concept we employed the potent iron chelator DFO (desferrioxamine) conjugated with starch to form...

View article titled, Relocalized redox-active lysosomal iron is an important mediator of oxidative-stress-induced DNA damage

Articles

Changes in the proteolytic activities of proteasomes and lysosomes in human fibroblasts produced by serum withdrawal, amino-acid deprivation and confluent conditions

Graciela FUERTES, José Javier MARTÍN DE LLANO, Adoración VILLARROYA, A. Jennifer RIVETT, Erwin KNECHT

Biochem J (2003) 375 (1): 75–86.

https://doi.org/10.1042/bj20030282

Published: 01 October 2003

... used pharmacological inhibitors of protein degradation were first analysed in detail. By choosing specific inhibitors of lysosomes and proteasomes, it was observed that together both pathways accounted for 80% or more of the degradation of cell proteins. With lysosomal inhibitors, it was found...

View article titled, Changes in the proteolytic activities of proteasomes and lysosomes in human fibroblasts produced by serum withdrawal, amino-acid deprivation and confluent conditions

Articles

Evidence for the interaction of the hereditary haemochromatosis protein, HFE, with the transferrin receptor in endocytic compartments

Paige S. DAVIES, An-Sheng ZHANG, Emily L. ANDERSON, Cindy N. ROY, Michael A. LAMPSON, Timothy E. McGRAW, Caroline A. ENNS

Biochem J (2003) 373 (1): 145–153.

https://doi.org/10.1042/bj20030202

Published: 01 July 2003

... interact in endosome compartments. Cells expressing a chimaera of HFE protein with the cytoplasmic domain of lysosomal-associated membrane protein 1 (LAMP1) in place of its own (HFE–LAMP) show a decrease in the half-life of the TfR. This implies that the interaction between HFE and TfR in endosomes targets...

View article titled, Evidence for the interaction of the hereditary haemochromatosis protein, HFE, with the transferrin receptor in endocytic compartments

Articles

3-Aminopropanal, formed during cerebral ischaemia, is a potent lysosomotropic neurotoxin

Wei LI, Xi-Ming YUAN, Svetlana IVANOVA, Kevin J. TRACEY, John W. EATON, Ulf T. BRUNK

Biochem J (2003) 371 (2): 429–436.

https://doi.org/10.1042/bj20021520

Published: 15 April 2003

... that 3-AP, having the structure of a weak lysosomotropic base, might concentrate within lysosomes, making these organelles a probable focus of initial toxicity. Indeed, 3-AP leads to lysosomal rupture of D384 glioma cells, a process which clearly precedes caspase activation and apoptotic cell death...

View article titled, 3-Aminopropanal, formed during cerebral ischaemia, is a potent lysosomotropic neurotoxin

Articles

Inhibitors of vacuolar H+-ATPase impair the preferential accumulation of daunomycin in lysosomes and reverse the resistance to anthracyclines in drug-resistant renal epithelial cells

Zahia OUAR, Marcelle BENS, Caroline VIGNES, Marc PAULAIS, Claudine PRINGEL, Jocelyne FLEURY, Françoise CLUZEAUD, Roger LACAVE, Alain VANDEWALLE

Biochem J (2003) 370 (1): 185–193.

https://doi.org/10.1042/bj20021411

Published: 15 February 2003

... contributes to the mechanisms of drug resistance. The function of the acidic lysosomes can be altered in MDR cells, and so we investigated the effects of lysosomotropic agents on the secretion of lysosomal enzymes and on the intracellular distribution of the weak-base anthracycline daunomycin in drug...

View article titled, Inhibitors of vacuolar H+-ATPase impair the preferential accumulation of daunomycin in lysosomes and reverse the resistance to anthracyclines in drug-resistant renal epithelial cells

Articles

Endolysosomal proteolysis and its regulation

Ché S. PILLAY, Edith ELLIOTT, Clive DENNISON

Biochem J (2002) 363 (3): 417–429.

https://doi.org/10.1042/bj3630417

Published: 24 April 2002

... endocytosed material and redundant cellular components are hydrolysed. Endocytosed material tends to flow vectorially through the system, proceeding through the early endosome, the endosome carrier vesicle, the late endosome and the lysosome. Phagocytosis and autophagy provide alternative entry points...

View article titled, Endolysosomal proteolysis and its regulation

Articles

Identification of cathepsin L as a differentially expressed message associated with skeletal muscle wasting

Christiane DEVAL, Sylvie MORDIER, Christiane OBLED, Daniel BECHET, Lydie COMBARET, Didier ATTAIX, Marc FERRARA

Biochem J (2001) 360 (1): 143–150.

https://doi.org/10.1042/bj3600143

Published: 08 November 2001

... was isolated, confirmed as being overexpressed in septic skeletal muscle and identified as encoding the lysosomal cysteine endopeptidase cathepsin L. Northern- and Western-blot analysis of cathepsin L in gastrocnemius or tibialis anterior muscles of septic rats confirmed an elevation (up to 3-fold) of both...

View article titled, Identification of cathepsin L as a differentially expressed message associated with skeletal muscle wasting

Articles

Sphingosine-induced apoptosis is dependent on lysosomal proteases

Katarina KÅGEDAL, Ming ZHAO, Iréne SVENSSON, Ulf T. BRUNK

Biochem J (2001) 359 (2): 335–343.

https://doi.org/10.1042/bj3590335

Published: 08 October 2001

...Katarina KÅGEDAL; Ming ZHAO; Iréne SVENSSON; Ulf T. BRUNK We propose a new mechanism for sphingosine-induced apoptosis, involving relocation of lysosomal hydrolases to the cytosol. Owing to its lysosomotropic properties, sphingosine, which is also a detergent, especially when protonated...

View article titled, Sphingosine-induced apoptosis is dependent on lysosomal proteases

Articles

Cloning of a lymphatic peptide/histidine transporter

Kazuko SAKATA, Toshihide YAMASHITA, Mitsuyo MAEDA, Yoshinori MORIYAMA, Shoichi SHIMADA, Masaya TOHYAMA

Biochem J (2001) 356 (1): 53–60.

https://doi.org/10.1042/bj3560053

Published: 08 May 2001

... in situ hybridization. Ectopic expression of rPHT2 protein in HEK-293T cells and BHK cells was not found on the cell surface, but was found on the lysosomal membrane using light- and electron-microscopic analysis. Recombinant rPHT2 protein reconstituted into liposomes showed proton-dependent transport...

View article titled, Cloning of a lymphatic peptide/histidine transporter

Articles

Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation

Paula E. ROW, Barbara J. REAVES, Jan DOMIN, J. Paul LUZIO, Howard W. DAVIDSON

Biochem J (2001) 353 (3): 655–661.

https://doi.org/10.1042/bj3530655

Published: 25 January 2001

...-kinase (PI-3K), Vps34p, which is required for correct targeting of newly synthesized carboxypeptidase Y to the yeast vacuole. A probable mammalian Vps34p orthologue has been previously identified, but its function in the trafficking of lysosomal enzymes has not been resolved. To investigate the possible...

View article titled, Overexpression of a rat kinase-deficient phosphoinositide 3-kinase, Vps34p, inhibits cathepsin D maturation

Articles

Autophagosome-associated variant isoforms of cytosolic enzymes

Monica FENGSRUD, Camilla RAIBORG, Trond Olav BERG, Per Eivind STRØMHAUG, Takashi UENO, Egil S. ERICHSEN, Per O. SEGLEN

Biochem J (2000) 352 (3): 773–781.

https://doi.org/10.1042/bj3520773

Published: 08 December 2000

...Monica FENGSRUD; Camilla RAIBORG; Trond Olav BERG; Per Eivind STRØMHAUG; Takashi UENO; Egil S. ERICHSEN; Per O. SEGLEN In a search for autophagosome-associated proteins, two-dimensional gel separations of proteins from purified autophagosomes, postnuclear supernatant, cytosol, lysosomes...

View article titled, Autophagosome-associated variant isoforms of cytosolic enzymes

Articles

Glucose persistence on high-mannose oligosaccharides selectively inhibits the macroautophagic sequestration of N-linked glycoproteins

Eric OGIER-DENIS, Chantal BAUVY, Françoise CLUZEAUD, Alain VANDEWALLE, Patrice CODOGNO

Biochem J (2000) 345 (3): 459–466.

https://doi.org/10.1042/bj3450459

Published: 25 January 2000

...Eric OGIER-DENIS; Chantal BAUVY; Françoise CLUZEAUD; Alain VANDEWALLE; Patrice CODOGNO The macroautophagic-lysosomal pathway is a bulk degradative process for cytosolic proteins and organelles including the endoplasmic reticulum (ER). We have previously shown that the human colonic carcinoma HT-29...

View article titled, Glucose persistence on high-mannose oligosaccharides selectively inhibits the macroautophagic sequestration of N-linked glycoproteins

Articles

Subcellular localization of the Gαi3 protein and G alpha interacting protein, two proteins involved in the control of macroautophagy in human colon cancer HT-29 cells

Anne PETIOT, Eric OGIER-DENIS, Chantal BAUVY, Françoise CLUZEAUD, Alain VANDEWALLE, Patrice CODOGNO

Biochem J (1999) 337 (2): 289–295.

https://doi.org/10.1042/bj3370289

Published: 08 January 1999

...-29 cells. 27 5 1998 8 10 1998 4 11 1998 The Biochemical Society, London © 1999 1999 degradation endoplasmic reticulum GTPase lysosome regulator of G-protein signalling Biochem. J. (1999) 337, 289 295 (Printed in Great Britain) 289 Subcellular localization...

View article titled, Subcellular localization of the Gαi3 protein and G alpha interacting protein, two proteins involved in the control of macroautophagy in human colon cancer HT-29 cells

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