... powerful data from Drosophila in 2005 that extended the earlier studies in mammalian cells into the world of the fly [ 7 ]. Novel fly G-proteins were discerned, including a fly G o protein homologue of the mammalian counterpart identified by knockdown experiments in F9 cells [ 2 ]. Overexpression...

... in the membrane of living cells. To address this question, we stably transfected two mammalian cell lines (HEK-293 and CHO-K1) with mouse NAPE-PLD cDNA, and investigated the endogenous levels and compositions of NAPEs and NAEs in these cells, compared with mock-transfected cells, with the aid of GC-MS...

... of the parasite were obtained by monolayer culture of murine muscle L-6 cells grown in RPMI medium containing 10% fetal calf serum at 37 °C in 5% CO 2 and then purified as described previously [ 10 , 11 ]. mammalian cell prolyl oligopeptidase structural modelling Tc80 proteinase Trypanosoma cruzi...