...). In the present study we characterize Alg11 from yeast as a mannosyltransferase catalysing the sequential transfer of two α1,2-linked mannose residues from GDP-mannose to Man 3 GlcNAc 2 -PP-Dol and subsequently to Man 4 GlcNAc 2 -PP-Dol forming the Man 5 GlcNAc 2 -PP-Dol intermediate at the cytosolic side...

... A mannan contained β-1,2-linked mannose residues attached to α-1,3-linked mannose residues and α-1,6-linked branching mannose residues. Using a β-1,2-mannosyltransferase, we synthesized a three-β-1,2-linkage-containing mannoheptaose and used it as a reference oligosaccharide for 1 H-NMR assignment...

..., except pPIC9- CaMNN5 D284A HIS4 This study Candida albicans endocytosis iron-binding protein iron uptake mannosyltransferase Iron is an essential nutrient for almost all organisms. The facile nature of iron to gain and lose electrons enables it to participate as a co...

...)mannosyltransferase. In the present study, we prepared a series of ten novel prenyl-related photoactivatable probes based on benzophenone with lipophilic spacers replacing several internal isoprene units. These probes were excellent substrates for the recombinant PPM synthase Mt -Ppm1/D2 and, on photoactivation...

...Christian THIEL; Markus SCHWARZ; Martin HASILIK; Ulrike GRIEBEN; Folker HANEFELD; Ludwig LEHLE; Kurt von FIGURA; Christian KÖRNER Deficiency of the endoplasmic reticulum enzyme dolichyl-phosphate mannose (Dol-P-Man):Man 7 GlcNAc 2 -PP-dolichyl mannosyltransferase leads to a new type of congenital...

... encodes a PPM synthase and that the GDP-Man:polyprenol phosphate mannosyltransferase activity is catalysed by the second domain of the protein. To evaluate the possible role of domain 1 of Mt-Ppm1, enzymic assays were performed with membrane fractions (5 lg each) prepared from M. smegmatis harbouring...