...André Struglics; Maria Hansson Although it has been shown that aggrecanases are involved in aggrecan degradation, the role of MMP (matrix metalloproteinase) aggrecanolysis is less well studied. To investigate MMP proteolysis of human aggrecan, in the present study we used neoepitope antibodies...

... on the activity as an ionizable group. 1 To whom correspondence should be addressed (email [email protected] ). 10 6 2004 22 9 2004 15 10 2004 15 10 2004 The Biochemical Society, London 2005 enzyme activity matrix metalloproteinase matrilysin nitrotyrosine pH...

...Sara A. ILLMAN; Jorma KESKI-OJA; William C. PARKS; Jouko LOHI Epilysin (MMP-28) is a recently identified member of the matrix metalloproteinase (MMP) family. To explore the expression of epilysin in vivo and to gain insight into its biological functions, we have cloned the mouse epilysin cDNA...

...M. Margarida BERNARDO; Rafael FRIDMAN The matrix metalloproteinase (MMP)-2 has a crucial role in extracellular matrix degradation associated with cancer metastasis and angiogenesis. The latent form, pro-MMP-2, is activated on the cell surface by the membrane-tethered membrane type 1 (MT1)-MMP...

... metalloproteinase nucleoside diphosphate kinase Abbreviations used: AP, activator protein; DTT, dithiothreitol; EMSA, electrophoretic mobility-shift assay; GST, glutathione S-transferase; MC, mesangial cell; MMP, matrix metalloproteinase; MT1-MMP, membrane type 1-MMP; NF-κB, nuclear factor κB; ORF, open...

... secreted matrix metalloproteinases and high-density lipoprotein (HDL) components, we tested the above cell types by culturing them for 24h. HDL 3 (HDL subfractions with average sizes of between 8.44nm for HDL 3A and 7.62nm for HDL 3C ) were then incubated in their cell-free conditioned media. Proteolytic...

.... Bovine nasal cartilage cultured with either IL-1β or retinoic acid exhibited significant release of glycosaminoglycan (GAG). For both factors, aggrecan proteolysis occurred predominantly at the ‘aggrecanase’ site, with no evidence for the action of matrix metalloproteinases, and resulted...