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Articles

SNARE proteins: zip codes in vesicle targeting?

Seiichi Koike, Reinhard Jahn

Biochem J (2022) 479 (3): 273–288.

https://doi.org/10.1042/BCJ20210719

Published: 04 February 2022

... signals such as Rab GTPases and polyphosphoinositides that are recognized by tethering factors in the cytoplasm and that connect the vesicles with their respective destination compartment. The final step, membrane fusion, is mediated by SNARE proteins. SNAREs are connected to targeting signals...

View article titled, SNARE proteins: zip codes in vesicle targeting?

Articles

SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?

Herre Jelger Risselada, Andreas Mayer

Biochem J (2020) 477 (1): 243–258.

https://doi.org/10.1042/BCJ20190050

Published: 17 January 2020

..., which originates from substantial energetic barriers to membrane fusion [ 1 ]. To facilitate intracellular membrane fusion reactions in a controlled manner, proteinaceous fusion machineries have evolved. An important open question is whether protein fusion machineries actively pull the fusion reaction...

View article titled, SNAREs, tethers and SM proteins: how to overcome the final barriers to membrane fusion?

Articles

Munc18a clusters SNARE-bearing liposomes prior to trans -SNARE zippering

Matthew Grant Arnold, Pratikshya Adhikari, Baobin Kang, Hao Xu (徐昊)

Biochem J (2017) 474 (19): 3339–3354.

https://doi.org/10.1042/BCJ20170494

Published: 25 September 2017

...Matthew Grant Arnold; Pratikshya Adhikari; Baobin Kang; Hao Xu (徐昊) Sec1–Munc18 (SM) proteins co-operate with SNAREs {SNAP [soluble NSF ( N -ethylmaleimide-sensitive factor) attachment protein] receptors} to mediate membrane fusion in eukaryotic cells. Studies of Munc18a/Munc18-1/Stxbp1...

View article titled, Munc18a clusters SNARE-bearing liposomes prior to trans -SNARE zippering

Includes: Supplementary data

Articles

α-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking

Xiaochu Lou, Jaewook Kim, Brenden J. Hawk, Yeon-Kyun Shin

Biochem J (2017) 474 (12): 2039–2049.

https://doi.org/10.1042/BCJ20170200

Published: 06 June 2017

.... Correspondence: Yeon-Kyun Shin ( [email protected] ) 12 3 2017 8 5 2017 10 5 2017 11 5 2017 © 2017 The Author(s); published by Portland Press Limited on behalf of the Biochemical Society 2017 α-synuclein exocytosis membrane fusion single molecule SNARE proteins α...

View article titled, α-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking

Includes: Supplementary data

Articles

Role of Munc13-4 as a Ca 2+ -dependent tether during platelet secretion

Michael C. Chicka, Qiansheng Ren, David Richards, Lance M. Hellman, Jinchao Zhang, Michael G. Fried, Sidney W. Whiteheart

Biochem J (2016) 473 (5): 627–639.

https://doi.org/10.1042/BJ20151150

Published: 24 February 2016

...Michael C. Chicka; Qiansheng Ren; David Richards; Lance M. Hellman; Jinchao Zhang; Michael G. Fried; Sidney W. Whiteheart The Munc13 family of exocytosis regulators has multiple Ca 2+ -binding, C2 domains. Here, we probed the mechanism by which Munc13-4 regulates in vitro membrane fusion...

View article titled, Role of Munc13-4 as a Ca 2+ -dependent tether during platelet secretion

Articles

The proteins of exocytosis: lessons from the sperm model

Claudia Nora Tomes

Biochem J (2015) 465 (3): 359–370.

https://doi.org/10.1042/BJ20141169

Published: 22 January 2015

...-triggered membrane fusion. After fusion, the membrane around the secretory vesicle is incorporated into the plasma membrane and the granule releases its contents. The proteins involved in these processes belong to several highly conserved families: Rab GTPases, SNAREs (soluble NSF-attachment protein...

View article titled, The proteins of exocytosis: lessons from the sperm model

Articles

Early and late HIV-1 membrane fusion events are impaired by sphinganine lipidated peptides that target the fusion site

Yoel A. Klug, Avraham Ashkenazi, Mathias Viard, Ziv Porat, Robert Blumenthal, Yechiel Shai

Biochem J (2014) 461 (2): 213–222.

https://doi.org/10.1042/BJ20140189

Published: 26 June 2014

..., the lipid backbone of DHSM (dihydrosphingomyelin), and an HIV-1 envelope-derived peptide. Surprisingly, we discovered a partnership between the lipid and the peptide that impaired early membrane fusion events by reducing CD4 receptor lateral diffusion and HIV-1 fusion peptide-mediated lipid mixing. Moreover...

View article titled, Early and late HIV-1 membrane fusion events are impaired by sphinganine lipidated peptides that target the fusion site

Includes: Supplementary data

Articles

Singlet oxygen effects on lipid membranes: implications for the mechanism of action of broad-spectrum viral fusion inhibitors

Axel Hollmann, Miguel A. R. B. Castanho, Benhur Lee, Nuno C. Santos

Biochem J (2014) 459 (1): 161–170.

https://doi.org/10.1042/BJ20131058

Published: 14 March 2014

... and fluidity of membranes were assessed by fluorescence anisotropy and generalized polarization measurements. Finally, the ability to inhibit membrane fusion was evaluated using FRET. Our results indicate that 1 O 2 production by LJ001 and JL103 is able to induce several changes on membrane properties...

View article titled, Singlet oxygen effects on lipid membranes: implications for the mechanism of action of broad-spectrum viral fusion inhibitors

Includes: Supplementary data

Articles

Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes

Yoosoo Yang, Jung-Mi Oh, Paul Heo, Jae Yoon Shin, Byoungjae Kong, Jonghyeok Shin, Ji-Chun Lee, Jeong Su Oh, Kye Won Park, Choong Hwan Lee, Yeon-Kyun Shin, Dae-Hyuk Kweon

Biochem J (2013) 450 (3): 537–546.

https://doi.org/10.1042/BJ20121256

Published: 28 February 2013

... stopping membrane fusion between granules and plasma membrane. This led to the differential effect of polyphenols on degranulation of three distinct subsets of granules. These results suggest the possibility that formation of a variety of SNARE complexes in numerous cell types is controlled by polyphenols...

View article titled, Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes

Includes: Supplementary data

Articles

The yeast vacuolar Rab GTPase Ypt7p has an activity beyond membrane recruitment of the homotypic fusion and protein sorting–Class C Vps complex

Christopher Stroupe

Biochem J (2012) 443 (1): 205–211.

https://doi.org/10.1042/BJ20110687

Published: 14 March 2012

... of HOPS complex in lipid-mixing reactions is adjusted such that cardiolipin-free proteoliposomes with or without Ypt7p bind to equal amounts of HOPS. Ypt7p therefore must stimulate membrane fusion by a mechanism that is in addition to recruitment of HOPS to the membrane. This is the first demonstration...

View article titled, The yeast vacuolar Rab GTPase Ypt7p has an activity beyond membrane recruitment of the homotypic fusion and protein sorting–Class C Vps complex

Articles

Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed-conformation C-terminus of syntaxin 3

Ren-Wang Peng, Claudio Guetg, Eric Abellan, Martin Fussenegger

Biochem J (2010) 431 (3): 353–361.

https://doi.org/10.1042/BJ20100145

Published: 11 October 2010

...Ren-Wang Peng; Claudio Guetg; Eric Abellan; Martin Fussenegger The interaction between SM (Sec1/Munc18) and SNARE (soluble N -ethylmaleimide-sensitive factor-attachment receptor) proteins constitutes the core eukaryotic membrane fusion machinery which manages exocytosis by mediating fusion...

View article titled, Munc18b regulates core SNARE complex assembly and constitutive exocytosis by interacting with the N-peptide and the closed-conformation C-terminus of syntaxin 3

Articles

The hepatitis C virus and its hepatic environment: a toxic but finely tuned partnership

Marie Perrault, Eve-Isabelle Pécheur

Biochem J (2009) 423 (3): 303–314.

https://doi.org/10.1042/BJ20091000

Published: 12 October 2009

... of HCV transport to intracellular compartments, and the ultimate step of its entry is fusion, which probably takes place within endosomes. The mechanisms of HCV membrane fusion are still unclear, notably the nature of the fusion proteins is unknown and the contribution of HCV-associated lipoproteins...

View article titled, The hepatitis C virus and its hepatic environment: a toxic but finely tuned partnership

Articles

Characterization of two distinct binding modes between syntaxin 4 and Munc18c

Veronica Aran, Fiona M. Brandie, Alasdair R. Boyd, Theodoros Kantidakis, Elizabeth J. Rideout, Sharon M. Kelly, Gwyn W. Gould, Nia J. Bryant

Biochem J (2009) 419 (3): 655–660.

https://doi.org/10.1042/BJ20082293

Published: 14 April 2009

...-sensitive fusion protein-attachment protein receptor)-mediated membrane fusion. Understanding the conserved mechanisms by which SM proteins function in this process has proved challenging, largely due to an apparent lack of conservation of binding mechanisms between different SM–syntaxin pairs...

View article titled, Characterization of two distinct binding modes between syntaxin 4 and Munc18c

Articles

Phosphatidylinositol metabolism and membrane fusion

Dominic Poccia, Banafshé Larijani

Biochem J (2009) 418 (2): 233–246.

https://doi.org/10.1042/BJ20082105

Published: 11 February 2009

...Dominic Poccia; Banafshé Larijani Membrane fusion underlies many cellular events, including secretion, exocytosis, endocytosis, organelle reconstitution, transport from endoplasmic reticulum to Golgi and nuclear envelope formation. A large number of investigations into membrane fusion indicate...

View article titled, Phosphatidylinositol metabolism and membrane fusion

Includes: Multimedia, Supplementary data

Articles

Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans

James R. Johnson, Pawel Ferdek, Lu-Yun Lian, Jeff W. Barclay, Robert D. Burgoyne, Alan Morgan

Biochem J (2009) 418 (1): 73–80.

https://doi.org/10.1042/BJ20081956

Published: 28 January 2009

...James R. Johnson; Pawel Ferdek; Lu-Yun Lian; Jeff W. Barclay; Robert D. Burgoyne; Alan Morgan SNAREs (soluble N -ethylmaleimide-sensitive fusion protein-attachment protein receptors) are widely accepted to drive all intracellular membrane fusion events. SM (Sec1/Munc18-like) proteins bind to SNAREs...

View article titled, Binding of UNC-18 to the N-terminus of syntaxin is essential for neurotransmission in Caenorhabditis elegans

Includes: Multimedia, Supplementary data

Articles

A role for the syntaxin N-terminus

Mary Munson, Nia J. Bryant

Biochem J (2009) 418 (1): e1–e3.

https://doi.org/10.1042/BJ20082389

Published: 28 January 2009

...Mary Munson; Nia J. Bryant Intracellular membrane fusion steps in eukaryotes require the syntaxin family of SNARE (soluble N -ethylmaleimide-sensitive fusion protein-attachment protein receptor) proteins. Syntaxins are regulated at several levels through interactions with regulatory proteins...

View article titled, A role for the syntaxin N-terminus

Articles

The dimeric form of Ca 2+ -ATPase is involved in Ca 2+ transport in the sarcoplasmic reticulum

Makoto Ushimaru, Yoshihiro Fukushima

Biochem J (2008) 414 (3): 357–361.

https://doi.org/10.1042/BJ20071701

Published: 27 August 2008

... were mixed immediately prior to spectrum analysis. Solid and broken lines represent the emission spectra of the fusion and the control vesicles respectively. Ca 2+ -ATPase dimer FITC functional unit membrane fusion sarcoplasmic reticulum (SR) The possibility that an interaction...

View article titled, The dimeric form of Ca 2+ -ATPase is involved in Ca 2+ transport in the sarcoplasmic reticulum

Includes: Supplementary data

Articles

S-nitrosylation of syntaxin 1 at Cys 145 is a regulatory switch controlling Munc18-1 binding

Zoë J. Palmer, Rory R. Duncan, James R. Johnson, Lu-Yun Lian, Luciane V. Mello, David Booth, Jeff W. Barclay, Margaret E. Graham, Robert D. Burgoyne, Ian A. Prior, Alan Morgan

Biochem J (2008) 413 (3): 479–491.

https://doi.org/10.1042/BJ20080069

Published: 15 July 2008

... to the closed conformation of syntaxin 1a, thereby facilitating its engagement with the membrane fusion machinery. 1 To whom correspondence should be addressed (email [email protected] ). 8 1 2008 22 4 2008 2 5 2008 2 5 2008 © The Authors Journal compilation © 2008...

View article titled, S-nitrosylation of syntaxin 1 at Cys 145 is a regulatory switch controlling Munc18-1 binding

Includes: Supplementary data

Articles

The mechanism by which molecules containing the HIV gp41 core-binding motif HXXNPF inhibit HIV-1 envelope glycoprotein-mediated syncytium formation

Jing-He Huang, Heng-Wen Yang, Shuwen Liu, Jing Li, Shibo Jiang, Ying-Hua Chen

Biochem J (2007) 403 (3): 565–571.

https://doi.org/10.1042/BJ20061275

Published: 12 April 2007

... core in the late stage of the membrane-fusion process. 1 To whom correspondence should be addressed (email [email protected] or [email protected] ). 22 8 2006 11 1 2007 16 1 2007 16 1 2007 The Biochemical Society, London 2007 gene 3 protein...

View article titled, The mechanism by which molecules containing the HIV gp41 core-binding motif HXXNPF inhibit HIV-1 envelope glycoprotein-mediated syncytium formation

Articles

Transmembrane proteins are not required for early stages of nuclear envelope assembly

Corinne Ramos, Elvira R. Rafikova, Kamran Melikov, Leonid V. Chernomordik

Biochem J (2006) 400 (3): 393–400.

https://doi.org/10.1042/BJ20061218

Published: 28 November 2006

...Corinne Ramos; Elvira R. Rafikova; Kamran Melikov; Leonid V. Chernomordik All identified membrane fusion proteins are transmembrane proteins. In the present study, we explored the post-mitotic reassembly of the NE (nuclear envelope). The proteins that drive membrane rearrangements in NE assembly...

View article titled, Transmembrane proteins are not required for early stages of nuclear envelope assembly

Articles

pH-dependence of intermediate steps of membrane fusion induced by the influenza fusion peptide

Ding-Kwo Chang, Shu-Fang Cheng

Biochem J (2006) 396 (3): 557–563.

https://doi.org/10.1042/BJ20051920

Published: 29 May 2006

...Ding-Kwo Chang; Shu-Fang Cheng Membrane fusion mediated by the influenza-virus fusion protein is activated by low pH via a cascade of reactions. Some processes among them are irreversible, such as helix hairpin formation of the ectodomain, whereas others are reversible, such as exposure...

View article titled, pH-dependence of intermediate steps of membrane fusion induced by the influenza fusion peptide

Includes: Supplementary data

Articles

Self-assembly of SNARE fusion proteins into star-shaped oligomers

Colin RICKMAN, Kuang HU, Joe CARROLL, Bazbek DAVLETOV

Biochem J (2005) 388 (1): 75–79.

https://doi.org/10.1042/BJ20041818

Published: 10 May 2005

... (synaptosome-associated protein of 25 kDa) reside on the plasma membrane, whereas synaptobrevin resides on synaptic vesicles prior to fusion. The SNARE motifs of the three proteins form a helical bundle which probably drives membrane fusion. Since studies in vivo suggested an importance for multiple SNARE...

View article titled, Self-assembly of SNARE fusion proteins into star-shaped oligomers

Includes: Supplementary data

Articles

Nuclear envelope assembly is promoted by phosphoinositide-specific phospholipase C with selective recruitment of phosphatidylinositol-enriched membranes

Richard D. BYRNE, Teresa M. BARONA, Marie GARNIER, Grielof KOSTER, Matilda KATAN, Dominic L. POCCIA, Banafshé LARIJANI

Biochem J (2005) 387 (2): 393–400.

https://doi.org/10.1042/BJ20040947

Published: 05 April 2005

... To whom correspondence should be addressed (email [email protected] ). 7 6 2004 10 11 2004 19 11 2004 19 11 2004 The Biochemical Society, London 2005 diacylglycerol electrospray ionization mass spectrometry membrane fusion nuclear envelope...

View article titled, Nuclear envelope assembly is promoted by phosphoinositide-specific phospholipase C with selective recruitment of phosphatidylinositol-enriched membranes

Articles

The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex

Crestina L. BEITES, Kristen A. CAMPBELL, William S. TRIMBLE

Biochem J (2005) 385 (2): 347–353.

https://doi.org/10.1042/BJ20041090

Published: 07 January 2005

.../PAGE followed by Western blotting. docking filament membrane fusion secretion septin SNARE complex During the exocytosis of synaptic vesicles at the nerve terminal a host of presynaptic proteins ultimately interact to allow release of neurotransmitters into the synaptic cleft...

View article titled, The septin Sept5/CDCrel-1 competes with α-SNAP for binding to the SNARE complex

Articles

TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Mica OHARA-IMAIZUMI, Chiyono NISHIWAKI, Toshiteru KIKUTA, Shintaro NAGAI, Yoko NAKAMICHI, Shinya NAGAMATSU

Biochem J (2004) 381 (1): 13–18.

https://doi.org/10.1042/BJ20040434

Published: 22 June 2004

... exocytosis insulin release membrane fusion pancreatic β-cell total internal reflection fluorescence microscopy (TIRFM) Imaging techniques are powerful tools for detecting vesicle trafficking in live cells and they have provided significant advances in understanding the mechanism of exocytosis [ 1...

View article titled, TIRF imaging of docking and fusion of single insulin granule motion in primary rat pancreatic β-cells: different behaviour of granule motion between normal and Goto–Kakizaki diabetic rat β-cells

Includes: Multimedia, Supplementary data

Articles

Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Nils J. FÆRGEMAN, Søren FEDDERSEN, Janne K. CHRISTIANSEN, Morten K. LARSEN, Roger SCHNEITER, Christian UNGERMANN, Kudzai MUTENDA, Peter ROEPSTORFF, Jens KNUDSEN

Biochem J (2004) 380 (3): 907–918.

https://doi.org/10.1042/bj20031949

Published: 15 June 2004

... electrospray ionization mass spectrometry (ESI—MS) membrane fusion soluble N -ethylmaleimide-sensitive fusion protein attachment protein receptor (SNARE) vacuole Abbreviations used: ACBP, acyl-CoA-binding protein; ALP, alkaline phosphatase; API, aminopeptidase I; CPY, carboxypeptidase Y; DHS...

View article titled, Acyl-CoA-binding protein, Acb1p, is required for normal vacuole function and ceramide synthesis in Saccharomyces cerevisiae

Articles

In vitro fusion between Saccharomyces cerevisiae secretory vesicles and cytoplasmic-side-out plasma membrane vesicles

Lorena ARRASTUA, Eider SAN SEBASTIAN, Ana F. QUINCOCES, Claude ANTONY, Unai UGALDE

Biochem J (2003) 370 (2): 641–649.

https://doi.org/10.1042/bj20021736

Published: 01 March 2003

... methods developed in this study. 1 To whom correspondence should be addressed (e-mail [email protected] ). 6 11 2002 15 11 2002 15 11 2002 The Biochemical Society, London ©2003 2003 mathematical analysis membrane fusion octadecylrhodamine B-chloride (R18) secretion...

View article titled, In vitro fusion between Saccharomyces cerevisiae secretory vesicles and cytoplasmic-side-out plasma membrane vesicles

Articles

Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum

Aleksandra BOGDANOVIC, Nelly BENNETT, Sylvie KIEFFER, Mathilde LOUWAGIE, Takahiro MORIO, Jérôme GARIN, Michel SATRE, Franz BRUCKERT

Biochem J (2002) 368 (1): 29–39.

https://doi.org/10.1042/bj20020845

Published: 15 November 2002

... syntaxin 8 t-SNARE complex is associated with incoming endocytic material. Key words: endocytosis, macropinocytosis, membrane fusion. the internal pH concomitantly returns to 6.5. Egestion of undigested material occurs from these post-lysosomal compart- ments through exocytosis [9,10]. Eukaryotic membrane...

View article titled, Syntaxin 7, syntaxin 8, Vti1 and VAMP7 (vesicle-associated membrane protein 7) form an active SNARE complex for early macropinocytic compartment fusion in Dictyostelium discoideum

Articles

Role of synaptotagmin in Ca2+-triggered exocytosis

Ward C. TUCKER, Edwin R. CHAPMAN

Biochem J (2002) 366 (1): 1–13.

https://doi.org/10.1042/bj20020776

Published: 15 August 2002

... in a variety of cell types. Here we provide an overview of the members of this gene family, with particular emphasis on the question of whether and how synaptotagmin I functions during the final stages of membrane fusion: does it regulate the Ca 2+ -triggered opening and dilation of fusion pores? Biochem. J...

View article titled, Role of synaptotagmin in Ca2+-triggered exocytosis

Articles

Palmitoylation of the 25-kDa synaptosomal protein (SNAP-25) in vitro occurs in the absence of an enzyme, but is stimulated by binding to syntaxin

Michael VEIT

Biochem J (2000) 345 (1): 145–151.

https://doi.org/10.1042/bj3450145

Published: 17 December 1999

... with a physiological binding partner. It suggests further that palmitoylation of SNAP-25 requires the a-helical conformation of the protein, which is induced by binding to syntaxin 1. Key words: membrane fusion, neurosecretion, palmitoyl- coenzyme A, SNARE complex, synaptobrevin. palmitoylation often is a dynamic...

View article titled, Palmitoylation of the 25-kDa synaptosomal protein (SNAP-25) in vitro occurs in the absence of an enzyme, but is stimulated by binding to syntaxin

Articles

GLUT4 trafficking in insulin-stimulated rat adipose cells: evidence that heterotrimeric GTP-binding proteins regulate the fusion of docked GLUT4-containing vesicles

Cynthia M. FERRARA, Samuel W. CUSHMAN

Biochem J (1999) 343 (3): 571–577.

https://doi.org/10.1042/bj3430571

Published: 25 October 1999

... 21201-1524, U.S.A. 30 4 1999 26 7 1999 17 8 1999 The Biochemical Society, London © 1999 1999 adenosine exocytosis G-proteins isopreterenol membrane fusion Biochem. J. (1999) 343, 571 577 (Printed in Great Britain) 571 GLUT4 trafficking in insulin-stimulated rat...

View article titled, GLUT4 trafficking in insulin-stimulated rat adipose cells: evidence that heterotrimeric GTP-binding proteins regulate the fusion of docked GLUT4-containing vesicles

Articles

Molecular aspects of the endocytic pathway

Michael J. CLAGUE

Biochem J (1998) 336 (2): 271–282.

https://doi.org/10.1042/bj3360271

Published: 01 December 1998

... at the molecular level, of which studies of cargo selection, vesicle budding and membrane fusion events comprise the major part. Progress in this area has been driven by two approaches, yeast genetics and in vitro or cell-free assays, which reconstitute particular transport steps and allow biochemical manipulation...

View article titled, Molecular aspects of the endocytic pathway

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