..., the mechanism of action of CGA-N9 was investigated. We found that CGA-N9 induced the depolarization of the cell membrane and uptake of calcium ions into the cytosol and mitochondria. With the disruption of the mitochondrial membrane potential, the generation of intracellular reactive oxygen species (ROS...

... Biochemical Society 2013 Ca 2+ binding chimaeric protein Cl − channel membrane potential TMEM16 TMEM16A and TMEM16B, also known as ANO (anoctamin) 1 and ANO2 respectively, are major components of CaCCs (Ca 2+ -activated Cl − channels), a class of ion channels involved in various...

... of cell respiratory control, which reports the rate of ATP production, the proton leak rate, the coupling efficiency, the maximum respiratory rate, the respiratory control ratio and the spare respiratory capacity. Measurements of membrane potential provide useful additional information. Measurement...

...-binding sites and undergo modifications, its precise function is not known. In the present study we generated a cell line from RAW 264.7 murine macrophages that has a more than 80% reduced level of Vb. Functional analysis of these cells showed a loss of CcO activity, membrane potential and less ability...

...) p.p.m. 31 P NMR: δ 25.57 p.p.m. (+)ESI-MS (positive ion electrospray ionization MS) found m/z =529.153 (C 28 H 35 PI + requires m/z =529.152). lipophilic cation membrane potential mitochondria mitochondrial targeting mitoquinone (MitoQ) Mitochondria are central to many aspects...

... and mitochondrial membrane potential in rat islet cells and clonal pancreatic β-cells (INS-1). Saturation of the Ca 2+ signal occurred between 5 and 6 mM MeS, while secretion reached its maximum at 15 mM, suggesting operation of a K ATP -channel-independent pathway. Additional responses to MeS and glucose included...

... compartments such as the mitochondrial matrix, driven by the membrane potential. This property is used to visualize mitochondria, to deliver therapeutic molecules to mitochondria and to measure the membrane potential. In theory, lipophilic dications have a number of advantages over monocations for these tasks...

...) are associated with disorders involving tissues that are characterized by a high-energy demand, including the central nervous system, as well as skeletal and cardiac muscles [ 8 ]. ATPase 6 subunit F 1 F 0 ATPase membrane potential mitochondria mitochondrial DNA (mtDNA) point mutation proton transport...

... mitochondrial activity. A model is proposed using the known passive transport of pyruvate by UCP1. 1 To whom correspondence should be addressed (email [email protected] ). glycolysis membrane potential mitochondria pyruvate reactive oxygen species uncoupling protein (UCP) The Biochemical...

...-dimensional isoelectric focusing/SDS/PAGE; (ii) JNK-induced cytochrome c release, in a process independent of the permeability transition of the inner mitochondrial membrane (imPT) and insensitive to cyclosporin A; (iii) JNK mediated a partial collapse of the mitochondrial inner-membrane potential (Δψm...

...Feng-Yi WAN; Yi-Nan WANG; Guo-Jiang ZHANG The influence of oxidation of membrane thiol groups on lysosomal proton permeability was studied by measuring lysosomal pH with FITC-conjugated dextran, determining the membrane potential with 3,3′-dipropylthiadicarbocyanine iodide and monitoring...

... µ M diazoxide, but the continuous spiking activity was suppressed by 500 µ M diazoxide. Pyruvate failed to initiate insulin release (5–20mM) or to depolarize the membrane potential. ATP production in isolated β-cell mitochondria was detected as accumulation of ATP in the medium during incubation...

... at very high levels in mitochondria of white adipocytes and is strongly induced in the course of 3T3-L1 adipogenesis. To determine the consequences of the presence of mDIC on the mitochondrial membrane potential, we transiently expressed mDIC in 293-T cells. Overexpression of mDIC leads to significant...

...]). 2 To whom correspondence should be addressed ( [email protected] ). 15 3 1999 17 5 1999 22 6 1999 The Biochemical Society, London © 1999 1999 erythrocyte enzymes glycolysis intracellular pH membrane potential nuclear magnetic resonance Biochem. J...