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Articles

Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG–SecDF–YajC–YidC

Joanna Komar, Sara Alvira, Ryan J. Schulze, Remy Martin, Jelger A. Lycklama a Nijeholt, Sarah C. Lee, Tim R. Dafforn, Gabriele Deckers-Hebestreit, Imre Berger, Christiane Schaffitzel, Ian Collinson

Biochem J (2016) 473 (19): 3341–3354.

https://doi.org/10.1042/BCJ20160545

Published: 27 September 2016

... membrane insertion factors. In bacteria, the membrane protein ‘insertase’ YidC ushers TMHs through a lateral gate of SecY to the bilayer. YidC is also thought to incorporate proteins into the membrane independently of SecYEG. Here, we show the bacterial holo-translocon (HTL) — a supercomplex of SecYEG...

View articletitled, Membrane protein insertion and assembly by the bacterial holo-translocon SecYEG–SecDF–YajC–YidC

Includes: Supplementary data

Articles

The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme

Zhenbo Cao, Marcel van Lith, Lorna J. Mitchell, Marie Anne Pringle, Kenji Inaba, Neil J. Bulleid

Biochem J (2016) 473 (7): 851–858.

https://doi.org/10.1042/BJ20151223

Published: 29 March 2016

..., it is possible that proteins that undergo oxidative folding donate electrons required for the catalysis of bacterial VKOR, as is the case with the DsbA–DsbB disulfide bond formation system in Escherichia coli [ 7 ]. endoplasmic reticulum membrane protein redox-sensitive green fluorescent protein (GFP...

View articletitled, The membrane topology of vitamin K epoxide reductase is conserved between human isoforms and the bacterial enzyme

Articles

Molecular and structural transmembrane determinants critical for embedding claudin-5 into tight junctions reveal a distinct four-helix bundle arrangement

Jan Rossa, Jonas Protze, Christian Kern, Anna Piontek, Dorothee Günzel, Gerd Krause, Jörg Piontek

Biochem J (2014) 464 (1): 49–60.

https://doi.org/10.1042/BJ20140431

Published: 23 October 2014

.... claudin homology modelling membrane protein mutagenesis tight junction 2 These authors share last authorship and correspondence may be addressed to either of them (email [email protected] or [email protected] ). All manual manipulations, optimizations of ECL2 and TM models...

View articletitled, Molecular and structural transmembrane determinants critical for embedding claudin-5 into tight junctions reveal a distinct four-helix bundle arrangement

Includes: Supplementary data

Articles

New insights into the functions of PIGF, a protein involved in the ethanolamine phosphate transfer steps of glycosylphosphatidylinositol biosynthesis

Matthew J. Stokes, Yoshiko Murakami, Yusuke Maeda, Taroh Kinoshita, Yasu S. Morita

Biochem J (2014) 463 (2): 249–256.

https://doi.org/10.1042/BJ20140541

Published: 22 September 2014

...-phosphatidylinositol membrane protein metabolism PIGF Trypanosoma brucei Phosphatidylinositol-anchored proteins and glycans are found in evolutionarily diverse organisms including bacteria, protozoa, fungi, plants and animals. The structure of eukaryotic glycosylphosphatidylinositol (GPI) was first...

View articletitled, New insights into the functions of PIGF, a protein involved in the ethanolamine phosphate transfer steps of glycosylphosphatidylinositol biosynthesis

Articles

Conserved structure and domain organization among bacterial Slc26 transporters

Emma L. R. Compton, Kimberly Page, Heather E. Findlay, Michael Haertlein, Martine Moulin, Ulrich Zachariae, David G. Norman, Frank Gabel, Arnaud Javelle

Biochem J (2014) 463 (2): 297–307.

https://doi.org/10.1042/BJ20130619

Published: 22 September 2014

.... membrane protein protein purification protein structure Slc26/SulP small-angle neutron scattering transporter The Slc26A proteins are a ubiquitous family of secondary anion transporters conserved from bacteria to man [ 1 ] which belong to the amino acid–polyamine–organocation (APC) superfamily...

View articletitled, Conserved structure and domain organization among bacterial Slc26 transporters

Includes: Supplementary data

Articles

Early and late HIV-1 membrane fusion events are impaired by sphinganine lipidated peptides that target the fusion site

Yoel A. Klug, Avraham Ashkenazi, Mathias Viard, Ziv Porat, Robert Blumenthal, Yechiel Shai

Biochem J (2014) 461 (2): 213–222.

https://doi.org/10.1042/BJ20140189

Published: 26 June 2014

...Yoel A. Klug; Avraham Ashkenazi; Mathias Viard; Ziv Porat; Robert Blumenthal; Yechiel Shai Lipid-conjugated peptides have advanced the understanding of membrane protein functions and the roles of lipids in the membrane milieu. These lipopeptides modulate various biological systems such as viral...

View articletitled, Early and late HIV-1 membrane fusion events are impaired by sphinganine lipidated peptides that target the fusion site

Includes: Supplementary data

Articles

Detergent-free purification of ABC (ATP-binding-cassette) transporters

Sonali Gulati, Mohammed Jamshad, Timothy J. Knowles, Kerrie A. Morrison, Rebecca Downing, Natasha Cant, Richard Collins, Jan B. Koenderink, Robert C. Ford, Michael Overduin, Ian D. Kerr, Timothy R. Dafforn, Alice J. Rothnie

Biochem J (2014) 461 (2): 269–278.

https://doi.org/10.1042/BJ20131477

Published: 26 June 2014

... and are involved in numerous diseases, but study of the structure and function of these proteins is often hampered by their large size and membrane location. Membrane protein purification usually utilizes detergents to solubilize the protein from the membrane, effectively removing it from its native lipid...

View articletitled, Detergent-free purification of ABC (ATP-binding-cassette) transporters

Includes: Supplementary data

Articles

Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cells

Andrew T. Hutchinson, Ansha Malik, Mark B. Berkahn, Mark Agostino, Joyce To, Jessica L. Tacchi, Steven P. Djordjevic, Lynne Turnbull, Cynthia B. Whitchurch, Allen B. Edmundson, Darren R. Jones, Robert L. Raison, Paul A. Ramsland

Biochem J (2013) 454 (3): 479–489.

https://doi.org/10.1042/BJ20130575

Published: 29 August 2013

... ). 2 Retired. 24 4 2013 13 6 2013 4 7 2013 4 7 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 free immunoglobulin light chain membrane protein multiple myeloma protein aggregation MM (multiple myeloma) is a haematological...

View articletitled, Formation of assemblies on cell membranes by secreted proteins: molecular studies of free λ light chain aggregates found on the surface of myeloma cells

Includes: Supplementary data

Articles

Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co 2+ /Mg 2+ transport

Nurhuda Nordin, Albert Guskov, Terri Phua, Newsha Sahaf, Yu Xia, Siyan Lu, Hojjat Eshaghi, Said Eshaghi

Biochem J (2013) 451 (3): 365–374.

https://doi.org/10.1042/BJ20121745

Published: 12 April 2013

... equilibrated with GF buffer. The desalted eluate underwent a final purification step with Superdex 200 16/60 (GE Healthcare) equilibrated with GF buffer. channel Co 2+ transport gating mechanism membrane protein metal ion homoeostasis Mg 2+ transport Mg 2+ and Co 2+ are essential ions...

View articletitled, Exploring the structure and function of Thermotoga maritima CorA reveals the mechanism of gating and ion selectivity in Co 2+ /Mg 2+ transport

Includes: Supplementary data

Articles

Regulation of Bacillus subtilis DesK thermosensor by lipids

Mariana Martín, Diego de Mendoza

Biochem J (2013) 451 (2): 269–275.

https://doi.org/10.1042/BJ20121825

Published: 28 March 2013

... 29 1 2013 © The Authors Journal compilation © 2013 Biochemical Society 2013 DesK histidine kinase lipid regulation membrane protein Cell membranes are composed of a lipid bilayer, containing proteins that either span the bilayer or interact with the lipids on either side...

View articletitled, Regulation of Bacillus subtilis DesK thermosensor by lipids

Articles

Protein–protein interactions between the bilirubin-conjugating UDPglucuronosyltransferase UGT1A1 and its shorter isoform 2 regulatory partner derived from alternative splicing

Mélanie Rouleau, Pierre Collin, Judith Bellemare, Mario Harvey, Chantal Guillemette

Biochem J (2013) 450 (1): 107–114.

https://doi.org/10.1042/BJ20121594

Published: 24 January 2013

... The Authors Journal compilation © 2013 Biochemical Society 2013 alternative splice product dominant-negative regulation membrane protein protein–protein interaction uridine diphosphate-glucuronosyltransferase (UGT) The functions of most proteins depend on continuous interactions with other...

View articletitled, Protein–protein interactions between the bilirubin-conjugating UDPglucuronosyltransferase UGT1A1 and its shorter isoform 2 regulatory partner derived from alternative splicing

Articles

Functional analysis of membranous F o - a subunit of F 1 F o -ATP synthase by in vitro protein synthesis

Yutetsu Kuruma, Toshiharu Suzuki, Sakurako Ono, Masasuke Yoshida, Takuya Ueda

Biochem J (2012) 442 (3): 631–638.

https://doi.org/10.1042/BJ20111284

Published: 24 February 2012

... Biochemical Society 2012 a subunit cell-free protein synthesis F 1 F o -ATP synthase (F 1 F o ) H + -pump membrane protein proteoliposome F 1 F o (F 1 F o -ATP synthase) is ubiquitously found in the membranes of bacteria, chloroplasts and mitochondria, and synthesizes most...

View articletitled, Functional analysis of membranous F o - a subunit of F 1 F o -ATP synthase by in vitro protein synthesis

Articles

Determinants of the enzymatic activity and the subcellular localization of aspartate N-acetyltransferase

Gaëlle Tahay, Elsa Wiame, Donatienne Tyteca, Pierre J. Courtoy, Emile Van Schaftingen

Biochem J (2012) 441 (1): 105–112.

https://doi.org/10.1042/BJ20111179

Published: 14 December 2011

... should be addressed (email [email protected] ). 1 7 2011 20 9 2011 21 9 2011 21 9 2011 © The Authors Journal compilation © 2012 Biochemical Society 2012 N -acetylaspartate N-acetyltransferase brain metabolism endoplasmic reticulum membrane protein...

View articletitled, Determinants of the enzymatic activity and the subcellular localization of aspartate N-acetyltransferase

Includes: Supplementary data

Articles

A combined action of pulmonary surfactant proteins SP-B and SP-C modulates permeability and dynamics of phospholipid membranes

Elisa Parra, Lara H. Moleiro, Ivan López-Montero, Antonio Cruz, Francisco Monroy, Jesús Pérez-Gil

Biochem J (2011) 438 (3): 555–564.

https://doi.org/10.1042/BJ20110681

Published: 26 August 2011

... pore membrane protein 1 To whom correspondence should be addressed (email [email protected] ). Pulmonary surfactant, a membrane-based lipid–protein complex, is strictly required for breathing in pulmonated organisms. The presence of a surfactant layer at the respiratory surface...

View articletitled, A combined action of pulmonary surfactant proteins SP-B and SP-C modulates permeability and dynamics of phospholipid membranes

Articles

Why does mitochondrial complex I have so many subunits?

Judy Hirst

Biochem J (2011) 437 (2): e1–e3.

https://doi.org/10.1042/BJ20110918

Published: 28 June 2011

... are important for enzyme stability. This commentary aims to put this suggestion into context. 1 email [email protected] 24 5 2011 31 5 2011 © The Authors Journal compilation © 2011 Biochemical Society 2011 accessory subunit membrane protein mitochondrion NADH dehydrogenase...

View articletitled, Why does mitochondrial complex I have so many subunits?

Articles

A scaffold of accessory subunits links the peripheral arm and the distal proton-pumping module of mitochondrial complex I

Heike Angerer, Klaus Zwicker, Zibiernisha Wumaier, Lucie Sokolova, Heinrich Heide, Mirco Steger, Silke Kaiser, Esther Nübel, Bernhard Brutschy, Michael Radermacher, Ulrich Brandt, Volker Zickermann

Biochem J (2011) 437 (2): 279–288.

https://doi.org/10.1042/BJ20110359

Published: 28 June 2011

...Heike Angerer; Klaus Zwicker; Zibiernisha Wumaier; Lucie Sokolova; Heinrich Heide; Mirco Steger; Silke Kaiser; Esther Nübel; Bernhard Brutschy; Michael Radermacher; Ulrich Brandt; Volker Zickermann Mitochondrial NADH:ubiquinone oxidoreductase (complex I) is a very large membrane protein complex...

View articletitled, A scaffold of accessory subunits links the peripheral arm and the distal proton-pumping module of mitochondrial complex I

Includes: Supplementary data

Articles

Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the β-domain pore

Yujia Zhai, Kai Zhang, Yanwu Huo, Yanshi Zhu, Qiangjun Zhou, Jiuwei Lu, Isobel Black, Xiaoyun Pang, Aleksander W. Roszak, Xujia Zhang, Neil W. Isaacs, Fei Sun

Biochem J (2011) 435 (3): 577–587.

https://doi.org/10.1042/BJ20101548

Published: 13 April 2011

... design. autotransporter Bordetella pertussis membrane protein outer membrane translocation passenger domain secretion trypsin-accessibility assay Whooping cough (pertussis) is a highly contagious acute respiratory illness of humans caused by the Gram-negative bacterial pathogen...

View articletitled, Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the β-domain pore

Includes: Supplementary data

Articles

Characterization of a novel peroxisome membrane protein essential for conversion of isopenicillin N into cephalosporin C

Ricardo V. Ullán, Fernando Teijeira, Susana M. Guerra, Inmaculada Vaca, Juan F. Martín

Biochem J (2010) 432 (2): 227–236.

https://doi.org/10.1042/BJ20100827

Published: 12 November 2010

...-fluorescence-labelled CefP protein co-localized in the peroxisomes with the control peroxisomal protein. In summary, CefP is a peroxisomal membrane protein probably involved in the import of IPN into the peroxisomes where it is converted into PenN by the two-component CefD1/CefD2 protein system. Recently...

View articletitled, Characterization of a novel peroxisome membrane protein essential for conversion of isopenicillin N into cephalosporin C

Includes: Supplementary data

Articles

Similarities and differences in the structure and function of 4.1G and 4.1R 135 , two protein 4.1 paralogues expressed in erythroid cells

Wataru Nunomura, Kengo Kinoshita, Marilyn Parra, Philippe Gascard, Xiuli An, Narla Mohandas, Yuichi Takakuwa

Biochem J (2010) 432 (2): 407–416.

https://doi.org/10.1042/BJ20100041

Published: 12 November 2010

.... In the present study, we performed detailed characterization of the interaction of 4.1G with various erythroid membrane proteins and the regulation of these interactions by calcium-saturated calmodulin. Like both isoforms of 4.1R, 4.1G bound to band 3, glycophorin C, CD44, p55 and calmodulin. While both 4.1G...

View articletitled, Similarities and differences in the structure and function of 4.1G and 4.1R 135 , two protein 4.1 paralogues expressed in erythroid cells

Includes: Supplementary data

Articles

Band 3 Edmonton I, a novel mutant of the anion exchanger 1 causing spherocytosis and distal renal tubular acidosis

Carmen Chu, Naomi Woods, Nunghathai Sawasdee, Helene Guizouarn, Bernard Pellissier, Franck Borgese, Pa-thai Yenchitsomanus, Manjula Gowrishankar, Emmanuelle Cordat

Biochem J (2010) 426 (3): 379–388.

https://doi.org/10.1042/BJ20091525

Published: 24 February 2010

... G701D mutation. To our knowledge, this case is the first reported instance of G701D in a non-Asian family. A total of 40 μg of total RBC membrane protein per lane was loaded on to an 8% (w/v) polyacrylamide gel and separated by SDS/PAGE, followed by Coomassie Blue staining. For Western blot...

View articletitled, Band 3 Edmonton I, a novel mutant of the anion exchanger 1 causing spherocytosis and distal renal tubular acidosis

Articles

Molecular identification of aspartate N-acetyltransferase and its mutation in hypoacetylaspartia

Elsa Wiame, Donatienne Tyteca, Nathalie Pierrot, François Collard, Mustapha Amyere, Gaëtane Noel, Jonathan Desmedt, Marie-Cécile Nassogne, Miikka Vikkula, Jean-Noël Octave, Marie-Françoise Vincent, Pierre J. Courtoy, Eugen Boltshauser, Emile van Schaftingen

Biochem J (2010) 425 (1): 127–139.

https://doi.org/10.1042/BJ20091024

Published: 14 December 2009

... should be addressed (email [email protected] ). 6 7 2009 28 8 2009 6 10 2009 6 10 2009 © The Authors Journal compilation © 2010 Biochemical Society 2010 endoplasmic reticulum (ER) hypoacetylaspartia membrane protein N -acetylaspartate (NAA) N...

View articletitled, Molecular identification of aspartate N-acetyltransferase and its mutation in hypoacetylaspartia

Includes: Supplementary data

Articles

EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the ethylene receptor ETR1

Melanie M. A. Bisson, Andrea Bleckmann, Silke Allekotte, Georg Groth

Biochem J (2009) 424 (1): 1–6.

https://doi.org/10.1042/BJ20091102

Published: 23 October 2009

...Melanie M. A. Bisson; Andrea Bleckmann; Silke Allekotte; Georg Groth Genetic studies have identified the membrane protein EIN2 (ethylene insensitive 2) as a central component of ethylene signalling in Arabidopsis . In addition, EIN2 might take part in multiple hormone signalling pathways...

View articletitled, EIN2, the central regulator of ethylene signalling, is localized at the ER membrane where it interacts with the ethylene receptor ETR1

Includes: Supplementary data

Articles

Effects of phosphatidylethanolamine glycation on lipid–protein interactions and membrane protein thermal stability

Valeria Levi, Ana M. Villamil Giraldo, Pablo R. Castello, Juan P. F. C. Rossi, F. Luis González Flecha

Biochem J (2008) 416 (1): 145–152.

https://doi.org/10.1042/BJ20080618

Published: 28 October 2008

... by a complex organization of lipids and proteins interacting and forming domains of different size and stability. In the present study, we analyse the effects of glycation on the interactions between membrane proteins and lipids. The phospholipid affinity for the transmembrane surface of the PMCA (plasma...

View articletitled, Effects of phosphatidylethanolamine glycation on lipid–protein interactions and membrane protein thermal stability

Includes: Supplementary data

Articles

MARCKS-like protein, a membrane protein identified for its expression in developing neural retina, plays a role in regulating retinal cell proliferation

Jing Zhao, Tomonori Izumi, Kazuto Nunomura, Shinya Satoh, Sumiko Watanabe

Biochem J (2007) 408 (1): 51–59.

https://doi.org/10.1042/BJ20070826

Published: 29 October 2007

...Jing Zhao; Tomonori Izumi; Kazuto Nunomura; Shinya Satoh; Sumiko Watanabe Membrane proteins are expressed in a specific manner in developing tissues, and characterization of these proteins is valuable because it allows them to be used as cell surface markers. Furthermore, they are potentially...

View articletitled, MARCKS-like protein, a membrane protein identified for its expression in developing neural retina, plays a role in regulating retinal cell proliferation

Articles

Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis

Kyu-Ho Park, Catherine Berrier, Florence Lebaupain, Bernard Pucci, Jean-Luc Popot, Alexandre Ghazi, Francesca Zito

Biochem J (2007) 403 (1): 183–187.

https://doi.org/10.1042/BJ20061473

Published: 13 March 2007

... solubilization, substitute for detergents to keep membrane proteins soluble, which generally improves their stability [Breyton, Chabaud, Chaudier, Pucci and Popot (2004) FEBS Lett. 564 , 312–318]. In the present study, we show that (hemi)fluorinated surfactants can be used for in vitro synthesis of membrane...

View articletitled, Fluorinated and hemifluorinated surfactants as alternatives to detergents for membrane protein cell-free synthesis

Articles

Structural and functional analysis of the Na + /H + exchanger

Emily R. Slepkov, Jan K. Rainey, Brian D. Sykes, Larry Fliegel

Biochem J (2007) 401 (3): 623–633.

https://doi.org/10.1042/BJ20061062

Published: 12 January 2007

...Emily R. Slepkov; Jan K. Rainey; Brian D. Sykes; Larry Fliegel The mammalian NHE (Na + /H + exchanger) is a ubiquitously expressed integral membrane protein that regulates intracellular pH by removing a proton in exchange for an extracellular sodium ion. Of the nine known isoforms of the mammalian...

View articletitled, Structural and functional analysis of the Na + /H + exchanger

Includes: Supplementary data

Articles

Biosynthesis of the dystonia-associated AAA + ATPase torsinA at the endoplasmic reticulum

Anna C. Callan, Sandra Bunning, Owen T. Jones, Stephen High, Eileithyia Swanton

Biochem J (2007) 401 (2): 607–612.

https://doi.org/10.1042/BJ20061313

Published: 21 December 2006

..., a single membrane spanning domain, and its C-terminus located in the ER (endoplasmic reticulum) lumen. However, in the present study we show that torsinA is not in fact an integral membrane protein. Instead we find that the mature protein associates peripherally with the ER membrane, most likely through...

View articletitled, Biosynthesis of the dystonia-associated AAA + ATPase torsinA at the endoplasmic reticulum

Articles

Two glucose/xylose transporter genes from the yeast Candida intermedia : first molecular characterization of a yeast xylose–H + symporter

Maria José Leandro, Paula Gonçalves, Isabel Spencer-Martins

Biochem J (2006) 395 (3): 543–549.

https://doi.org/10.1042/BJ20051465

Published: 11 April 2006

.... intermedia unveiled a group of putative, previously uncharacterized, fungal monosaccharide–H + symporters, comprising proteins from several different yeasts and filamentous fungi. Candida intermedia membrane protein sugar transport xylose/glucose–H + symport Ethanol that is to be used...

View articletitled, Two glucose/xylose transporter genes from the yeast Candida intermedia : first molecular characterization of a yeast xylose–H + symporter

Articles

Computational evidence for protein-mediated fatty acid transport across the sarcolemma

Mark W. J. M. Musters, James B. Bassingthwaighte, Natal A. W. van Riel, Ger J. van der Vusse

Biochem J (2006) 393 (3): 669–678.

https://doi.org/10.1042/BJ20050869

Published: 13 January 2006

... investigated the effects of a sarcolemma-associated albumin receptor and a hypothetical FA-TFPC on the kinetics of trans-sarcolemmal FA transport rates. The Biochemical Society, London 2006 extracellular matrix fatty acid membrane protein modelling plasma albumin To meet the energy...

View articletitled, Computational evidence for protein-mediated fatty acid transport across the sarcolemma

Articles

Trafficking defects of the Southeast Asian ovalocytosis deletion mutant of anion exchanger 1 membrane proteins

Joanne C. Cheung, Emmanuelle Cordat, Reinhart A. F. Reithmeier

Biochem J (2005) 392 (3): 425–434.

https://doi.org/10.1042/BJ20051076

Published: 06 December 2005

... Society, London 2005 anion exchanger 1 (AE1) distal renal tubular acidosis (dRTA) kidney membrane protein Southeast Asian ovalocytosis (SAO) The SLC4 gene family encodes membrane proteins that mediate bicarbonate transport in mammalian cells, and is important for pH homoeostasis...

View articletitled, Trafficking defects of the Southeast Asian ovalocytosis deletion mutant of anion exchanger 1 membrane proteins

Articles

Topology of transmembrane segments 1–4 in the human chloride/bicarbonate anion exchanger 1 (AE1) by scanning N-glycosylation mutagenesis

Joanne C. Cheung, Jing Li, Reinhart A. F. Reithmeier

Biochem J (2005) 390 (1): 137–144.

https://doi.org/10.1042/BJ20050315

Published: 09 August 2005

... form a re-entrant loop in human AE1. 1 To whom correspondence should be addressed (email [email protected] ). The Biochemical Society, London 2005 anion exchanger Band 3 membrane protein N-glycosylation ovalocytosis topology Human AE1 (anion exchanger 1...

View articletitled, Topology of transmembrane segments 1–4 in the human chloride/bicarbonate anion exchanger 1 (AE1) by scanning N-glycosylation mutagenesis

Articles

A misassembled transmembrane domain of a polytopic protein associates with signal peptide peptidase

Samuel G. CRAWSHAW, Bruno MARTOGLIO, Suzanna L. MEACOCK, Stephen HIGH

Biochem J (2004) 384 (1): 9–17.

https://doi.org/10.1042/BJ20041216

Published: 09 November 2004

... is mediated by its second transmembrane domain, and propose that this interaction may contribute to the recognition of misassembled transmembrane domains during membrane protein quality control at the ER. 1 Present address: European Patent Office, Landsbergerstrasse 30, 80339 München, Germany...

View articletitled, A misassembled transmembrane domain of a polytopic protein associates with signal peptide peptidase

Articles

Palmitoylation is not required for trafficking of human anion exchanger 1 to the cell surface

Joanne C. CHEUNG, Reinhart A. F. REITHMEIER

Biochem J (2004) 378 (3): 1015–1021.

https://doi.org/10.1042/bj20030847

Published: 15 March 2004

... transfected HEK-293 cells or COS7 cells with [ 3 H]palmitic acid failed to produce any detectable AE1 palmitoylation. These results suggest that AE1 is not palmitoylated in HEK-293 or COS7 cells and can traffic to the plasma membrane. anion exchanger 1 (AE1) biosynthesis cysteine membrane protein...

View articletitled, Palmitoylation is not required for trafficking of human anion exchanger 1 to the cell surface

Articles

Topology of the Erwinia chrysanthemi oligogalacturonate porin KdgM

Teijo PELLINEN, Helena AHLFORS, Nicolas BLOT, Guy CONDEMINE

Biochem J (2003) 372 (2): 329–334.

https://doi.org/10.1042/bj20030027

Published: 01 June 2003

... 2 2003 The Biochemical Society, London ©2003 2003 β-barrel membrane protein pectin degradation Abbreviations used: LB, Luria–Bertani; PEO-MAB; EZ-Link TM PEO maleimide-activated biotin. Biochem. J. (2003) 372, 329 334 (Printed in Great Britain) 329 Topology of the Erwinia...

View articletitled, Topology of the Erwinia chrysanthemi oligogalacturonate porin KdgM

Articles

Impaired trafficking of human kidney anion exchanger (kAE1) caused by hetero-oligomer formation with a truncated mutant associated with distal renal tubular acidosis

Janne A. QUILTY, Emmanuelle CORDAT, Reinhart A.F. REITHMEIER

Biochem J (2002) 368 (3): 895–903.

https://doi.org/10.1042/bj20020574

Published: 15 December 2002

..., oligosaccharide processing and pulse—chase experiments. The 901 stop mutants were able to bind to an inhibitor affinity resin, suggesting that these mutant membrane proteins were not grossly misfolded. Co-expression of wild-type and mutant kAE1 or AE1 resulted in intracellular retention of the wild-type proteins...

View articletitled, Impaired trafficking of human kidney anion exchanger (kAE1) caused by hetero-oligomer formation with a truncated mutant associated with distal renal tubular acidosis

Articles

Thermal denaturation of influenza virus and its relationship to membrane fusion

Richard M. EPAND, Raquel F. EPAND

Biochem J (2002) 365 (3): 841–848.

https://doi.org/10.1042/bj20020290

Published: 01 August 2002

... of a destabilization of the trimeric form of HA. This temperature is lower than that required for the major endothermic peak observed in DSC experiments. The results demonstrate that there is no kinetic- ally trapped high-energy form of HA at neutral pH. Key words: fusion energy, membrane protein, protein denatur...

View articletitled, Thermal denaturation of influenza virus and its relationship to membrane fusion

Articles

Lysosome-associated protein transmembrane 4α (LAPTM4α) requires two tandemly arranged tyrosine-based signals for sorting to lysosomes

Douglas L. HOGUE, Colin NASH, Victor LING, Tom C. HOBMAN

Biochem J (2002) 365 (3): 721–730.

https://doi.org/10.1042/bj20020205

Published: 01 August 2002

... marker lysosomal glycoprotein 120. Although a number of membrane proteins that localize to endosomes/lysosomes contain more than one independently functioning sorting signal, to our knowledge, LAPTM4α is the first example of a membrane protein that requires two tandemly arranged tyrosine-based sorting...

View articletitled, Lysosome-associated protein transmembrane 4α (LAPTM4α) requires two tandemly arranged tyrosine-based signals for sorting to lysosomes

Articles

Cytoplasmic domains of the reduced folate carrier are essential for trafficking, but not function

Heather SADLISH, Frederick M.R. WILLIAMS, Wayne F. FLINTOFF

Biochem J (2002) 364 (3): 777–786.

https://doi.org/10.1042/bj20011361

Published: 15 June 2002

... be addressed (e-mail [email protected] ). 20 9 2001 7 12 2001 9 4 2002 The Biochemical Society, London ©2002 2002 biogenesis intracellular domain membrane protein polytopic topogenesis Abbreviations used: BODIPY ® , 4,4-difluoro-4-bora-3a,4a-diaza- s -indacene; (E)GFP...

View articletitled, Cytoplasmic domains of the reduced folate carrier are essential for trafficking, but not function

Articles

Expression and purification of functional recombinant meningococcal transferrin-binding protein A

Jonathan S. OAKHILL, Christopher L. JOANNOU, Susan K. BUCHANAN, Andrew R. GORRINGE, Robert W. EVANS

Biochem J (2002) 364 (3): 613–616.

https://doi.org/10.1042/bj20020500

Published: 15 June 2002

... Institutes of Health, 50 South Drive, Bethesda, MD 20892-8030, U.S.A. 2 To whom correspondence should be addressed (e-mail [email protected] ). 28 3 2002 23 4 2002 25 4 2002 The Biochemical Society, London ©2002 2002 iron membrane protein Neisseria meningitidis...

View articletitled, Expression and purification of functional recombinant meningococcal transferrin-binding protein A

Articles

Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry

Dan BLAKEY, Andrew LEECH, Gavin H. THOMAS, Graham COUTTS, Kim FINDLAY, Mike MERRICK

Biochem J (2002) 364 (2): 527–535.

https://doi.org/10.1042/bj20011761

Published: 01 June 2002

...Dan BLAKEY; Andrew LEECH; Gavin H. THOMAS; Graham COUTTS; Kim FINDLAY; Mike MERRICK The Amt family of high-affinity ammonium transporters is a family of integral membrane proteins that are found in archaea, bacteria, fungi, plants and animals. Furthermore, the family has recently been extended...

View articletitled, Purification of the Escherichia coli ammonium transporter AmtB reveals a trimeric stoichiometry

Articles

Ecto-protein kinase substrate p120 revealed as the cell-surface-expressed nucleolar phosphoprotein Nopp140: a candidate protein for extracellular Ca2+-sensing

Dieter KÜBLER

Biochem J (2001) 360 (3): 579–587.

https://doi.org/10.1042/bj3600579

Published: 10 December 2001

...Dieter KÜBLER A variety of cell membrane proteins become phosphorylated in their ecto-domains by cell-surface protein kinase (ecto-PK) activities, as detected in a broad spectrum of cell types. This study reports the isolation and identification of a frequent ecto-PK substrate, ecto-p120, using...

View articletitled, Ecto-protein kinase substrate p120 revealed as the cell-surface-expressed nucleolar phosphoprotein Nopp140: a candidate protein for extracellular Ca2+-sensing

Articles

Superficial disposition of the N-terminal region of the surfactant protein SP-C and the absence of specific SP-B–SP-C interactions in phospholipid bilayers

Inés PLASENCIA, Antonio CRUZ, Cristina CASALS, Jesús PÉREZ-GIL

Biochem J (2001) 359 (3): 651–659.

https://doi.org/10.1042/bj3590651

Published: 25 October 2001

... of the dependence of RET on the protein density excluded specific SP-B Dns-SP-C associations. Key words: pulmonary surfactant, lipid protein interactions, membrane protein, resonance energy transfer. during compression which leads to an enrichment in DPPC [8,9]. Furthermore, SP-C exhibits the ability to form...

View articletitled, Superficial disposition of the N-terminal region of the surfactant protein SP-C and the absence of specific SP-B–SP-C interactions in phospholipid bilayers

Articles

Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein

Dong CHENG, Rupalie L. MEEGALLA, Bokang HE, Debra A. CROMLEY, Jeffery T. BILLHEIMER, Peter R. YOUNG

Biochem J (2001) 359 (3): 707–714.

https://doi.org/10.1042/bj3590707

Published: 25 October 2001

.... Key words: differential splicing, membrane protein, protein complex, triacylglycerol. gene encoding DGAT [Dgat were created by Farese and co- workers [10]. Dgat mice seem to have a fully differentiated fat mass and normal serum triacylglycerol levels, indicating that lipoprotein assembly is normal...

View articletitled, Human acyl-CoA:diacylglycerol acyltransferase is a tetrameric protein

Articles

Functional role of polar amino acid residues in Na+/H+ exchangers

Christine A. WIEBE, Emily R. DiBATTISTA, Larry FLIEGEL

Biochem J (2001) 357 (1): 1–10.

https://doi.org/10.1042/bj3570001

Published: 25 June 2001

...Christine A. WIEBE; Emily R. DiBATTISTA; Larry FLIEGEL Na + /H + exchangers are a family of ubiquitous membrane proteins. In higher eukaryotes they regulate cytosolic pH by removing an intracellular H + in exchange for an extracellular Na + . In yeast and Escherichia coli , Na + /H + exchangers...

View articletitled, Functional role of polar amino acid residues in Na+/H+ exchangers

Articles

Structural similarities of Na,K-ATPase and SERCA, the Ca2+-ATPase of the sarcoplasmic reticulum

Kathleen J. SWEADNER, Claudia DONNET

Biochem J (2001) 356 (3): 685–704.

https://doi.org/10.1042/bj3560685

Published: 08 June 2001

... proteins. On the whole, the similarity supports a shared fold, with some particular exceptions. Key words: cross-linking, crystal structure, membrane protein, protein folding, P-type ATPases. of about 100 kDa with usually ten predicted transmembrane spans and four intracellular loops that differ greatly...

View articletitled, Structural similarities of Na,K-ATPase and SERCA, the Ca2+-ATPase of the sarcoplasmic reticulum

Articles

An examination of how structural changes can affect the rate of electron transfer in a mutated bacterial photoreaction centre

Justin P. RIDGE, Paul K. FYFE, Katherine E. McAULEY, Marion E. VAN BREDERODE, Bruno ROBERT, Rienk VAN GRONDELLE, Neil W. ISAACS, Richard J. COGDELL, Michael R. JONES

Biochem J (2000) 351 (3): 567–578.

https://doi.org/10.1042/bj3510567

Published: 24 October 2000

... The Biochemical Society, London © 2000 2000 membrane protein mutagenesis photosynthesis reaction centre X-ray crystallography Biochem. J. (2000) 351, 567 578 (Printed in Great Britain) 567 An examination of how structural changes can affect the rate of electron transfer in a mutated bacterial...

View articletitled, An examination of how structural changes can affect the rate of electron transfer in a mutated bacterial photoreaction centre

Articles

Mutations in the reduced-folate carrier affect protein localization and stability

Heather SADLISH, Richard C. MURRAY, Frederick M. R. WILLIAMS, Wayne F. FLINTOFF

Biochem J (2000) 346 (2): 509–518.

https://doi.org/10.1042/bj3460509

Published: 22 February 2000

..., London © 2000 2000 methotrexate resistance membrane protein N-glycosylation protein degradation Biochem. J. (2000) 346, 509 518 (Printed in Great Britain) 509 Mutations in the reduced-folate carrier affect protein localization and stability Heather SADLISH, Richard C. MURRAY, Frederick M. R...

View articletitled, Mutations in the reduced-folate carrier affect protein localization and stability

Articles

Large-scale production and purification of functional recombinant bovine rhodopsin with the use of the baculovirus expression system

Corné H. W. KLAASSEN, Petra H. M. BOVEE-GEURTS, Godelieve L. J. DECALUWÉ, Willem J. DEGRIP

Biochem J (1999) 342 (2): 293–300.

https://doi.org/10.1042/bj3420293

Published: 24 August 1999

...Corné H. W. KLAASSEN; Petra H. M. BOVEE-GEURTS; Godelieve L. J. DECALUWÉ; Willem J. DEGRIP Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine...

View articletitled, Large-scale production and purification of functional recombinant bovine rhodopsin with the use of the baculovirus expression system

Articles

A shift in the equilibrium constant at the catalytic site of proton-translocating transhydrogenase: significance for a ‘binding-change’ mechanism

Jamie D. VENNING, J. B JACKSON

Biochem J (1999) 341 (2): 329–337.

https://doi.org/10.1042/bj3410329

Published: 08 July 1999

..., London © 1999 1999 membrane protein nicotinamide nucleotide proton pump Rhodospirillum rubrum stopped-flow kinetics Biochem. J. (1999) 341, 329 337 (Printed in Great Britain) 329 A shift in the equilibrium constant at the catalytic site of proton-translocating transhydrogenase : significance...

View articletitled, A shift in the equilibrium constant at the catalytic site of proton-translocating transhydrogenase: significance for a ‘binding-change’ mechanism

Articles

Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium

Gert ZIMMER, Frank OEFFNER, V. V MESSLING, Thomas TSCHERNIG, H GRÖNE, H KLENK, Georg HERRLER

Biochem J (1999) 341 (2): 277–284.

https://doi.org/10.1042/bj3410277

Published: 08 July 1999

... the corresponding human protein, a type-I integral membrane protein of 162 amino acids. Madin-Darby canine kidney cells transfected with the cDNA clone directed the cell-surface expression of a 36-kDa O-glycosylated sialoglycoprotein, gp36, and two minor isoforms of 28 and 70 kDa. gp36 has a broad tissue...

View articletitled, Cloning and characterization of gp36, a human mucin-type glycoprotein preferentially expressed in vascular endothelium

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