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1-7 of 7
Keywords: metalloenzyme
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Articles
Mechanism and catalytic strategy of the prokaryotic-specific GTP cyclohydrolase-IB
Available to PurchaseNaduni Paranagama, Shilah A. Bonnett, Jonathan Alvarez, Amit Luthra, Boguslaw Stec, Andrew Gustafson, Dirk Iwata-Reuyl, Manal A. Swairjo
Journal:
Biochemical Journal
Biochem J (2017) 474 (6): 1017–1039.
Published: 07 March 2017
... with the Econo-Safe scintillation cocktail (RPI), and counted on an LS6000 Series Scintillation Counter (Beckman). 7-deazaguanosine bacterial metabolism metalloenzyme modified nucleoside post-translational modification (PTM) transfer RNA (tRNA) GTP cyclohydrolase-I (GCYH-I; FolE) is a Zn 2...
Includes: Supplementary data
Articles
Structure and function of carbonic anhydrases
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2016) 473 (14): 2023–2032.
Published: 12 July 2016
... metalloenzyme X-ray crystallography The carbonic anhydrases (CAs, EC 4.2.1.1) belong to a superfamily of metalloenzymes which use as substrates some unusually chemically simple molecules/ions, as they catalyse the interconversion between CO 2 and bicarbonate [ 1 – 13 ]. Although this reaction may...
Articles
Metallophosphoesterases: structural fidelity with functional promiscuity
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2015) 467 (2): 201–216.
Published: 02 April 2015
... with a highly conserved structural core that allows embellishment to result in dramatic and niche-specific diversification of function. GpdQ metalloenzyme metallophosphoesterase Mre11 phosphoester Rv0805 Phosphoesters occupy a central role in biology. Their presence in diverse biomolecules...
Articles
Mechanistic studies of FosB: a divalent-metal-dependent bacillithiol-S-transferase that mediates fosfomycin resistance in Staphylococcus aureus
Available to PurchaseAlexandra A. Roberts, Sunil V. Sharma, Andrew W. Strankman, Shayla R. Duran, Mamta Rawat, Chris J. Hamilton
Journal:
Biochemical Journal
Biochem J (2013) 451 (1): 69–79.
Published: 14 March 2013
... should be addressed (email [email protected] ). 8 10 2012 14 12 2012 21 12 2012 21 12 2012 © The Authors Journal compilation © 2013 Biochemical Society 2013 antibiotic detoxification bacillithiol metalloenzyme substrate mimic The antibiotic fosfomycin [(1...
Includes: Supplementary data
Articles
Bacillus subtilis 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase revisited: resolution of two long-standing enigmas
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2005) 390 (2): 583–590.
Published: 23 August 2005
.... Metal analysis showed that B. subtilis DAHPS as isolated contained iron and zinc and is inactivated by dipicolinic acid; the inactive apoenzyme can be reactivated by bivalent metal ions, indicating that the enzyme is a metalloenzyme. The enzyme-bound metal is insensitive to EDTA treatment, leading...
Includes: Supplementary data
Articles
Distinct classes of glyoxalase I: metal specificity of the Yersinia pestis , Pseudomonas aeruginosa and Neisseria meningitidis enzymes
Available to Purchase
Journal:
Biochemical Journal
Biochem J (2004) 384 (1): 111–117.
Published: 09 November 2004
... establish the existence of a non-Zn 2+ -dependent grouping within the general category of GlxI enzymes. This second, established class of metal activation was formerly unidentified for this metalloenzyme. Amino acid sequence comparisons indicate a more extended peptide chain in the Zn 2+ -dependent forms...
Articles
Contribution of the active-site metal cation to the catalytic activity and to the conformational stability of phosphotriesterase: temperature- and pH-dependence
Available to PurchaseDaniel ROCHU, Nathalie VIGUIÉ, Frédérique RENAULT, David CROUZIER, Marie-Thérèse FROMENT, Patrick MASSON
Journal:
Biochemical Journal
Biochem J (2004) 380 (3): 627–633.
Published: 15 June 2004
... electrophoresis metalloenzyme phosphotriesterase protein stability thermal unfolding Abbreviations used: BCA, bicinchoninic acid; CE, capillary electrophoresis; CHES, 2-( N -cyclohexylamino)ethanesulphonic acid; IEF, isoelectric focusing; OP, organophosphorus compounds; PTE, phosphotriesterase; RMSD...
