... compressive load. Proteolysis of aggrecan by members of the proteinase family ADAMTS (A disintegrin-like and metalloproteinase domain with thrombospondin type 1 motif), was identified as an early step in the inexorable destruction of cartilage in osteoarthritis (OA). Of the investigated proteinases, ADAMTS5...

...Ngee H. Lim; Masahide Kashiwagi; Robert Visse; Jonathan Jones; Jan J. Enghild; Keith Brew; Hideaki Nagase We have reported previously that reactive-site mutants of N-TIMP-3 [N-terminal inhibitory domain of TIMP-3 (tissue inhibitor of metalloproteinases 3)] modified at the N-terminus, selectively...

...Sofie H. Willems; Christopher J. Tape; Peter L. Stanley; Neil A. Taylor; Ian G. Mills; David E. Neal; John McCafferty; Gillian Murphy ADAM17 (where ADAM is ‘a disintegrin and metalloproteinase’) can rapidly modulate cell-surface signalling events by the proteolytic release of soluble forms...

...Rosemary Bass; Dylan R. Edwards The ADAM disintegrin metalloproteinases (where ADAM is ‘a disintegrin and metalloproteinase’) are a family of transmembrane cell-surface proteins with essential roles in adhesion and proteolytic processing in all animals. The archetypal family member is ADAM17 {also...

...Cristina I. Caescu; Grace R. Jeschke; Benjamin E. Turk The metalloproteinases TACE [tumour necrosis factor α-converting enzyme; also known as ADAM17 (a disintegrin and metalloproteinase 17)] and ADAM10 are the primary enzymes responsible for catalysing release of membrane-anchored proteins from...

... with the inner and outer subdomain respectively [ 10 ]. CD9 CD81 CD82 CD151 metalloproteinase tetraspanin Another example of a potential tetraspanin ligand is DARC (Duffy antigen receptor for chemokines), identified as a CD82-binding molecule after screening a human normal prostate library...

... metalloproteinase and the furin-like proprotein convertases. Few of these processing interactions could have been predicted by analysing the presence of conserved consensus sequences in the proα1(V) chain. In the present study we opted for a cell approach that allows a straightforward identification of processing...

...), that are delivered into host cells by a third bacterial protein, PA (protective antigen). The two toxins act on a variety of cell types, disabling the immune system and inevitably killing the host. LF is an extraordinarily selective metalloproteinase that site-specifically cleaves MKKs (mitogen-activated protein...

...Wei-Man Wang; Gaoxiang Ge; N. H. Lim; Hideaki Nagase; Daniel S. Greenspan ADAMTS-2 is an extracellular metalloproteinase responsible for cleaving the N-propeptides of procollagens I–III; an activity necessary for the formation of collagenous ECM (extracellular matrix). The four TIMPs (tissue...

..., London 2006 anticoagulant C-type lectin metalloproteinase phospholipase A 2 snake venom three-finger toxin Snake venoms are complex mixtures of pharmacologically active proteins and polypeptides. They play an important role in incapacitating and immobilizing, as well as in digesting...

... (email [email protected] ). 15 3 2004 18 11 2004 19 11 2004 19 11 2004 The Biochemical Society, London 2005 aggrecanase angiogenesis extracellular matrix metalloproteinase proteoglycan tissue inhibitor of metalloproteinase (TIMP) Kuno et al. [ 1...

...-coupled receptors, primarily through metalloproteinase-dependent cleavage of a membrane-anchored EGF precursor. In the murine inner medullary collecting duct (mIMCD3) cell line, urea (200mM) induced prompt (1—5min) tyrosine phosphorylation of the EGF receptor. Pharmacological inhibition of EGF receptor...

... protein causes release of IGF. Pregnancy-associated plasma protein-A (PAPP-A) has recently been found to cleave IGFBP-4 in an IGF-dependent manner. To experimentally support the hypothesis that PAPP-A belongs to the metzincin superfamily of metalloproteinases, all containing the elongated zinc-binding...

... by a metalloproteinase and by protein kinase C (PKC) isoenzymes that do not require Ca 2+ for their activation. We show that XG-6 cells express PKC-δ, −η and −∊ isoenzymes. However, after stimulation with PMA, only PKC-δ and PKC-η are activated, as shown by their translocation to the membrane. Treatment with PMA induces...

... 2001 2001 collagen fibrils metalloproteinase propeptides spermatogonia Biochem. J. (2001) 355, 271 278 (Printed in Great Britain) 271 Transgenic mice with inactive alleles for procollagen N-proteinase (ADAMTS-2) develop fragile skin and male sterility Shi-Wu LI*, Machiko ARITA*1, Andrzej...