...Tyler B.J. Pinter; Martin J. Stillman Mammalian metallothioneins (MTs) bind up to seven Zn 2+ using a large number of cysteine residues relative to their small size and can act as zinc-chaperones. In metal-saturated Zn 7 –MTs, the seven zinc ions are co-ordinated tetrahedrally into two distinct...

... report in the present paper that PTEN (phosphatase and tensin homologue deleted on chromosome 10) associates with MTF-1 in the cells. These two proteins interact via the acidic domain of MTF-1 and the phosphatase/C2 domain of PTEN. Depletion of PTEN reduced MT (metallothionein) gene expression...

...Tomoki Kimura; Yong Li; Fumika Okumura; Norio Itoh; Tsuyoshi Nakanishi; Tomomichi Sone; Masakazu Isobe; Glen K. Andrews Mouse MT - I (metallothionein-I) transcription is regulated by MTF-1 (metal-response-element-binding transcription factor-1) which is recruited to the promoter in response to zinc...

... was developed. This MRE (metal-responsive element)–luciferase reporter contained four MREs from the mouse metallothionein 1A promoter upstream of the firefly luciferase open reading frame. Thus the MRE–luciferase reporter measured bioavailable cytosolic copper. Expression of hCTR1 resulted in strong activation...

...Fimmie Reinecke; Oksana Levanets; Yolanda Olivier; Roan Louw; Boitumelo Semete; Anne Grobler; Juan Hidalgo; Jan Smeitink; Antonel Olckers; Francois H. Van Der Westhuizen The role of MT (metallothionein) gene expression was investigated in rotenone-treated HeLa cells to induce a deficiency...

... iron, thus preventing Fenton-type reactions and ensuing peroxidation of lysosomal membranes. Because up-regulation of MTs (metallothioneins) also generates enhanced cellular resistance to oxidative stress, including X-irradiation, and MTs were found to be capable of iron binding in an acidic...

...Clemente Capasso; Vincenzo Carginale; Orlando Crescenzi; Daniela Di Maro; Roberta Spadaccini; Piero Andrea Temussi; Elio Parisi In previous studies, we showed that the chemical and dynamic properties of fish and mouse MTs (metallothioneins) present a number of distinctive differences linked...

...David NURY; Hervé CHABANON; Marilyne LEVADOUX-MARTIN; John HESKETH Localization of mRNAs provides a novel mechanism for synthesis of proteins close to their site of function. MT1 (metallothionein-1) is a small, metal-binding protein that is largely cytoplasmic but which can be found in the nucleus...

... metallothionein-I and zinc transporter-1 genes, whereas protein kinase C inhibitors exerted gene- and cell-type-specific effects on the metal regulation and basal expression of these two genes. A chromatin immunoprecipitation assay was used to demonstrate that none of these inhibitors prevent the metal-dependent...

...Yongyi BI; Richard D. PALMITER; Kristi M. WOOD; Qiang MA Phenolic antioxidants, such as tBHQ [2,5-di-(t-butyl)-1,4-hydroquinone], induce Mt1 (metallothionein 1) gene expression and accumulation of MT protein. Induction of Mt1 mRNA does not depend on protein synthesis, and correlates with oxidation...

...Lucía TAPIA; Mauricio GONZÁLEZ-AGÜERO; Mónica F. CISTERNAS; Miriam SUAZO; Verónica CAMBIAZO; Ricardo UAUY; Mauricio GONZÁLEZ MTs (metallothioneins) increase the resistance of cells to exposure to high Cu (copper) levels. Characterization of the MT–Cu complex suggests that MT has an important role...

...Klaus ZANGGER; Gong SHEN; Gülin ÖZ; James D. OTVOS; Ian M. ARMITAGE Upon storage under aerobic conditions metallothioneins (MTs) form a new species, which is characterized by a molecular mass approximately twice the size of monomeric MT and shifted 113/111 Cd- and 1 H-NMR resonances...

... gene sets could be discerned: signal transducer and activator of transciption-3 (STAT-3), suppressor of cytokine signalling-3 (SOCS-3), MT-II (metallothionein-II), the serine/threonine kinase fibroblast-growth-factor-inducible kinase (Fnk) and modulator recognition factor (MRF-1), which are immediate...